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| 1 | Key factors in developing the trinitrobenzene sulfonic acid-induced post-inflammatory irritable bowel syndrome model in rats显示文摘AIM:To investigate the key factors in developing the trinitrobenzene sulfonic acid(TNBS)-induced postinflammatory irritable bowel syndrome(PI-IBS)model in rats. METHODS:TNBS was administered to rats at the following conditions:(1)with different doses(20,10,5 mg/0.8 mL per rat);(2)with same dose in different concentrations(20 mg/rat,25,50 mg/mL);(3)in different ethanol percentage(25%,50%);and(4)at depth either 4 cm or 8 cm from anus.At 5 d and 4 wk after TNBS administration,inflammation severity and inflammation resolution were evaluated.At 4 and 8 wk after TNBS application,visceral hyperalgesia and enterochromaffin(EC)cell hyperplasia were assayed by abdominal withdrawal reflex test,silver staining and capillary electrophoresis. RESULTS:Our results showed that:(1)TNBS induced dose-dependent acute inflammation and inflammation resolution.At 5 d post TNBS,the pathological score and myeloperoxidase(MPO)activity in all TNBS treated rats were significantly elevated compared to that of the control(9.48±1.86,8.18±0.67,5.78± 0.77 vs 0,and 3.55±1.11,1.80±0.82,0.97±0.08 unit/mg vs 0.14±0.01 unit/mg,P<0.05).At 4 wk post TNBS,the pathological score in high and median dose TNBS-treated rats were still significantly higher than that of the control(1.52±0.38 and 0.80±0.35 vs 0,P<0.05);(2)Intracolonic TNBS administration position affected the persistence of visceral hyperalgesia.At 4 wk post TNBS,abdominal withdrawal reflex (AWR)threshold pressure in all TNBS-treated groups were decreased compared to that of the control(21.52 ±1.73 and 27.10±1.94 mmHg vs 34.44±1.89 mmHg,P<0.05).At 8 wk post TNBS,AWR threshold pressure in 8 cm administration group was still significantly decreased(23.33±1.33 mmHg vs 36.79±2.29 mmHg,P<0.05);(3)Ethanol percentage affected the TNBS-induced inflammation severity and visceral hyperalgesia.In TNBS-25%ethanol-treated group,the pathological score and MPO activity were significantly lowered compared to that of the TNBS-50%ethanoltreated group,while AWR threshold pressure were significantly elevated(36.33±0.61 mmHg vs 23.33±1.33 mmHg,P<0.05);and(4)TNBS(5 mg/0.8 mL per rat, in 50%ethanol,8 cm from anus)-treated rats recovered completely from the inflammation with acquired visceral hyperalgesia and EC cell hyperplasia at 4 wk after TNBS administration.CONCLUSION:TNBS dosage,concentration,intraco-lonic administration position,and ethanol percentage play important roles in developing visceral hyperalgesia and EC cell hyperplasia of TNBS-induced PI-IBS rats. | Hong-Yan Qin Hai-Tao Xiao Justin CY Wu Brian M Berman Joseph JY Sung Zhao-Xiang Bian | 2012 | World Journal of Gastroenterology2012，18，20： | 10 |
| 2 | Production of ACAT1 56-kDa isoform in human cells via trans-splicing involving the ampicillin resistance gene显示文摘拼接 Trans,包含劈开并且二个分开的抄本加入的一个过程,能在优核质扩展 transcriptome 和 proteome。拼接 trans 产生的妄想的 RNA 逐渐地在文学被描述。在自然环境和人的肠的抗菌素抵抗基因的普遍存在为公共健康正在成为重要挑战。某些抗菌素抵抗基因,例如氨比西林抵抗基因(安培 r ), 经常在 recombinant plasmids 被使用。直到现在,拼接 trans 包含 recombinant 导出 plasmid 的外长的抄本和内长的细胞的 RNA 没被报导。Acyl-CoA:cholesterol acyltransferase (ACAT1 ) 1 是涉及细胞的胆固醇动态平衡的关键酶。4.3-kb 人 ACAT1 妄想的 mRNA 能与不同酶的活动生产 50-kDa 和 56-kDa isoforms。这里,我们证明人的 ACAT1 56-kDa isoform 从通过安培 r ( asAmp )现在在普通安培 r -plasmids 和 4.3-kb 内长的 ACAT1 妄想的 mRNA ,它大概通过 interchromosomal 拼接trans 的一个优先的事件被处理。惊人地,与一个在上游的重新结合的神秘倡导者一起包含 asAmp 的 DNA 碎片和相应外长的 asAmp 抄本在人的房间被检测了。我们的调查结果使人的 ACAT1 56-kDa isoform 生产的机制清楚些,揭示一个外长内长的拼接 trans 系统,在 recombinant,导出 plasmid 的外长的抄本在人的房间与内长的细胞的 RNA 被连接,并且建议外长的 DNA 可能影响在 DNA 和 RNA 层次的人的基因表示。 | Guang-Jing Hu Jia Chen Xiao-Nan Zhao Jia-JiaXu Dong-Qing Guo Ming Lu Ming Zhu Ying Xiong Qin Li Catherine CY Chang Bao-Liang Song Ta-Yuan Chang Bo-Liang Li | 2013 | Cell Research2013，23，8： | 4 |
| 3 | Effect of angiotensin II type I receptor blocker losartan on bone deterioration in orchiectomized male hypertensive and normotensive rats显示文摘 | ZHANG Ya-feng QIN Ling Timothy CY Kwok Benson HY Yeung LI Guo-dong LIU Fan | 2013 | Chinese Medical Journal2013，，14： | 3 |
| 4 | ILK: a pseudokinase in the center stage of cell-matrix adhesion and signaling显示文摘 | QIN J WU CY | 2012 | Curr Opin Cell Biol2012，24，5： | 1 |
| 5 | The emerging role of microRNAs in immune cell development and differentiation显示文摘 | Liang TJ Qin CY | 2009 | APMIS2009，117，9： | 1 |
| 6 | The effect of TRAIL on the expression of multidrug resistant genes MDR1, LRP and GST-pi in drug-resistant gastric cancer cell SGC7901/VCR显示文摘 | Zhang KG Qin CY Wang H Q | 2012 | Hepatogastroenterology2012，59，120： | 1 |
| 7 | Chiral nanoporous metal-organic frameworks with high porosity as materials for drug delivery 显示文摘 | Sun CY Qin C Wang CG | 2011 | Adv Mater2011，23，47： | 1 |
| 8 | The effect of TRAIL on the expression of multidrug resistant genes MDR1, LRP and GST-~ in drug-resistant gastric cancer cell SGC7901/VCR 显示文摘 | Zhang KG Qin CY Wang HQ | 2012 | Hepatogastroenterology2012，59，120： | 1 |
| 9 | Influence of bone adaptation on tendon-to-bone healing in bone tunnel after anterior cruciate ligament reconstruction in a rabbit model显示文摘 | Wen CY Qin L Lee KM | | 0，，11： | 1 |
| 10 | The effect of TRAIL on theexpression of multidrug resistant genes MDR1,LRP and GST-π indrug-resistant gastric cancer cell SGC7901 /VCR 显示文摘 | Zhang KG Qin CY Wang HQ | 2012 | Hepatogastroenterology2012，59，120： | 1 |
| 11 | 显示文摘 | Hu CY Qin Z X Feng ZX | 2006 | Mat Sci and Eng B2006，128，： | 1 |
| 12 | Ginkgo biloba extract enhances chemotherapy sensitivity and reverses-chemoresistance through suppression of the KSRl-mediated ERK1/2 path- way in gastric cancer ceils 显示文摘 | Liu SQ Xu CY Qin MB | 2015 | Oncol Rep2015，33，6： | 1 |
| 13 | Grafted tendon healing in tibial tunnel is inferior to healing in femoral tunnel after anterior cruciate ligament reconstruction:a histomorphometric study in rabbits显示文摘 | Wen CY Qin L Lee KM | | 0，，： | 1 |
| 14 | Molecular characterization of the pL40 protein in Leptospira interrogans显示文摘 | Zhao W Chen CY Zhang XY Lai WQ Hu BY Zhao GP Qin JH Guo XK | 2009 | Can J Microbiol2009，55，6： | 1 |
| 15 | The effect of TRAIL on the expression of multidrug resistant genes MDR1, LRP and GST-π in drug-resistant gastric cancer cell SGC7901/VCR 显示文摘 | Zhang KG Qin CY Wang HQ | 2012 | Hepatogastro enterology2012，59，120： | 1 |
| 16 | Correlations of 3-eatenin, Ki67 and Her-2/neu with gastric cancer 显示文摘 | Wu HW Qin CY Huang JL | 2014 | Asian Pae J Trop Med2014，7，4： | 1 |
| 17 | Chiral nanoporous metal- organic frameworks with high porosity as materials for drug delivery显示文摘 | Sun CY Qin C Wang CG | 2011 | Adv Mater2011，23，47： | 1 |
| 18 | The effect of TRAIL on the expression of muhidrug resistant genes MDR1, LRP and GST-,u in drug-resistant gastric cancer cell SGC7901/VCR 显示文摘 | Zhang KG Qin CY Wang I-IQ | 2012 | Hepatogas- troenterology2012，59，120： | 1 |
| 19 | Metal - organic frameworks as potential drug delivery systems 显示文摘 | Sun CY Qin C Wang XL | 2012 | Expert Opin Drug Deliv2012，10，： | 1 |
| 20 | Synthesis and evaluation of the cell cycle arrest and CT DNA interaction properties of 413-amino4'- O-demethyl-4-deoxypodophyllotoxins显示文摘 | Liu JF Sang CY Qin WW | 2013 | Bioorg Med Chem2013，21，22： | 1 |
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