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| 1 | Generation of RAG 1- and 2-deficient rabbits by embryo microinjection of TALENs显示文摘 | Jun Song Juan Zhong Xiaogang Guo Yongqiang Chen Qingjian zou Jiao Huang Xiaoping Li Quanjun Zhang Zhiwu Jiang Chengcheng Tang Huaqiang Yang Tao Liu Peng Li Duanqing Pei Liangxue Lai | 2013 | Cell Research2013，23，8： | 15 |
| 2 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 3 | Expression of voltage.gated sodium channel Nav1.5 in non.metastatic colon cancer and its associations with estrogen receptor(ER)-βexpression and clinical outcomes显示文摘Background: Voltage-gated sodium channel 1.5(Nav1.5) potentially promotes the migratory and invasive behaviors of colon cancer cells. Hitherto, the prognostic significance of Nav1.5 expression remains undetermined. The present study aimed to explore the associations of Nav1.5 expression with clinical outcomes and estrogen receptor-β(ER-β)expression in non-metastatic colon cancer patients receiving radical resection.Methods: A total of 269 consecutive patients with pathologically confirmed stages Ⅰ-Ⅲ colon cancer who underwent radical resection were selected. Nav1.5 and ER-β expression was detected by using immunohistochemistry(IHC)on tissue microarray constructed from paraffin-embedded specimens. IHC score was determined according to the percentage and intensity of positively stained cells. Statistical analysis was performed with the X-tile method, k coefficient, Chi square test or Fisher's exact test, logistic regression, log-rank test, and Cox proportional hazards models.Results: We found that Nav1.5 was commonly expressed in tumor tissues with higher mean IHC score as compared with matched tumor-adjacent normal tissues(5.1 ± 3.5 vs. 3.5 ± 2.7, P < 0.001).The high expression of Nav1.5 in colon cancer tissues was associated with high preoperative carcinoembryonic antigen level [odds ratio(OR) = 2.980;95% confidential interval(CI)1.163-7.632; P = 0.023] and high ER-β expression(OR = 2.808; 95% CI 1.243-6.343;p = 0.00 3). Log-rank test results showed that high Nav1.5 expression contributed to a low 5-year disease-free survival(DFS) rate in colon cancer patients(77.2% vs. 92.1%, P = 0.048), especially in patients with high ER-β expression tumor(76.2% vs. 91.3%, P = 0.032). Analysis with Cox proportional hazards model demonstrated that high Nav1.5 expression[hazard ratio(HR) = 2.738; 95% CI 1.100-6.819;P = 0.030] and lymph node metastasis(HR = 2.633; 95% CI 1.632-4.248; P < 0.001) were prognostic factors for unfavorable DFS in colon cancer patients.Conclusions: High expression of Nav1.5 was associated with high expression of ER-β and indicated unfavorable oncologic prognosis in patients with non-metastatic colon cancer. | Jianhong Peng Qingjian Ou Xiaojun Wu Rongxin Zhang Qian Zhao Wu Jiang Zhenhai Lu Desen Wan Zhizhong Pan Yujing Fang | 2017 | Chinese Journal of Cancer2017，36，12： | 7 |
| 4 | Preparation of polymer-supported hydrated ferric oxide based on Donnan membrane effect and its application for arsenic removal显示文摘In the present study a novel technique was proposed to prepare a polymer-supported hydrated ferric oxide (D201-HFO) based on Donnan membrane effect by using a strongly basic anion exchanger D201 as the host material and FeCl3-HCl-NaCl solution as the reaction environment. D201-HFO was found to exhibit higher capacity for arsenic removal than a commercial sorbent Purolite ArsenX. Furthermore, it presents favorable adsorption selectivity for arsenic removal from aqueous solution, as well as satis- factory kinetics. Fixed-bed column experiments showed that arsenic sorption on D201-HFO could re- sult in concentration of this toxic metalloid element below 10 μg/L, which was the new maximum con- centration limit set recently by the European Commission and imposed by the US EPA and China. Also, the spent D201-HFO is amenable to efficient regeneration by NaOH-NaCl solution. | ZHANG QingJian PAN BingCai CHEN XinQing ZHANG WeiMing PAN BingJun ZHANG QuanXing L.LV X.S.ZHAO | 2008 | Science China Chemistry2008，51，4： | 4 |
| 5 | Effect of a combined inversion and plantarflexion surface on ankle kinematics and EMG activities in landing显示文摘目的:探寻下落在内翻结合跖屈表面时踝关节运动学参数和腓肠肌内侧、腓骨长肌、胫骨前肌的肌电活动情况。方法:12名运动员从30 cm高度下落时3种不同条件的表面:平面、25°内翻表面、25°内翻结合25°跖屈表面,每种条件分别采集5次落地。用单因素方差分析运动学参数,同时采用3×3双因素方差分析3种肌肉的积分肌电值(p<0.05)。结果:积分肌电值结果显示不同肌肉和表面之间存在交互作用。下落于平面时胫骨前肌的肌电活动高于另两种表面。相比较平面,下落于内翻表面的最大内翻角度和角速度明显提高,但腓骨长肌的肌电活动却相似。此外,下落于内翻结合跖屈的表面,其腓肠肌的积分肌电、踝关节的跖屈角度和内翻的关节活动度明显高于25°的内翻表面。结论:与单纯的内翻表面相比,内翻结合跖屈的表面在下落时会产生更不稳定的落地状况,从而更易造成踝关节扭伤。 | Divya Bhaskaran Michael Wortley Qingjian Chen Clare E. Milner Eugene C. Fitzhugh Songning Zhang | 2015 | Journal of Sport and Health Science2015，4，4： | 4 |
| 6 | Differential Inhibition of Nav1.7 and Neuropathic Pain by Hybridoma-Produced and Recombinant Monoclonal Antibodies that Target Nav1.7显示文摘The voltage-gated Na^+ channel subtype Nav1.7 is important for pain and itch in rodents and humans. We previously showed that a Nav1.7-targeting monoclonal antibody(SVmab) reduces Na+ currents and pain and itch responses in mice. Here, we investigated whether recombinant SVmab(rSVmab) binds to and blocks Nav1.7 similar to SVmab. ELISA tests revealed that SVmab was capable of binding to Nav1.7-expressing HEK293 cells,mouse DRG neurons, human nerve tissue, and the voltagesensor domain Ⅱ of Nav1.7. In contrast, rSVmab showed no or weak binding to Nav1.7 in these tests. Patch-clamp recordings showed that SVmab, but not rSVmab, markedly inhibited Na+ currents in Nav1.7-expressing HEK293 cells. Notably, electrical field stimulation increased the blocking activity of SVmab and rSVmab in Nav1.7-expressing HEK293 cells. SVmab was more effective than rSVmab in inhibiting paclitaxel-induced mechanical allodynia. SVmab also bound to human DRG neurons and inhibited their Na^+ currents. Finally, potential reasons for the differential efficacy of SVmab and rSVmab and future directions are discussed. | Sangsu Bang Jiho Yoo Xingrui Gong Di Liu Qingjian Han Xin Luo Wonseok Chang Gang Chen Sang-Taek Im Yong Ho Kim Judith A.Strong Ma-Zhong Zhang Jun-Ming Zhang Seok-Yong Lee Ru-Rong Ji | 2018 | Neuroscience Bulletin2018，34，1： | 2 |
| 7 | Generation of rat blood vasculature and hematopoietic cells in rat-mouse chimeras by blastocyst complementation显示文摘Interspecies chimera through blastocyst complementation could be an alternative approach to create human organs in animals by using human pluripotent stem cells.A mismatch of the major histocompatibility complex of vascular endothelial cells between the human and host animal will cause graft rejection in the transplanted organs.Therefore,to achieve a transplantable organ in animals without rejection,creation of vascular endothelial cells derived from humans within the organ is necessary.In this study,to explore whether donor xeno-pluripotent stem cells can compensate for blood vasculature in host animals,we generated rat-mouse chimeras by injection of rat embryonic stem cells(rESCs)into mouse blastocysts with deficiency of Flk-1 protein,which is associated with endothelial and hematopoietic cell development.We found that rESCs could differentiate into vascular endothelial and hematopoietic cells in the rat-mouse chimeras.The whole yolk sac(YS)of Flk-1^EGFP/ECFP rat-mouse chimera was full of rat blood vasculature.Rat genes related to vascular endothelial cells,arteries,and veins,blood vessels formation process,as well as hematopoietic cells,were highly expressed in the YS.Our results suggested that rat vascular endothelial cells could undergo proliferation,migration,and self-assembly to form blood vasculature and that hematopoietic cells could differentiate into B cells,T cells,and myeloid cells in rat-mouse chimeras,which was able to rescue early embryonic lethality caused by Flk-1 deficiency in mouse. | Xiaomin Wang Hui Shi Juanjuan Zhou Qingjian Zou Quanjun Zhang Shixue Gou Pengfei Chen Lisha Mou Nana Fan Yangyang Suo Zhen Ouyang Chengdan Lai Quanmei Yan Liangxue Lai | 2020 | Journal of Genetics and Genomics2020，47，5： | 2 |
| 8 | 地表面倾斜度与踝关节护具对垂直着地运动中地面反作用力、踝关节运动学和动力学的效应显示文摘现有研究文献尚无有关在着地过程中不同表面倾斜度和踝关节护具效应的运动学、动力学和地面反作用力的综合数据。通过对比25°斜面和平面的着地以及使用和不使用踝关节护具情况下来检测踝关节的生物力学特性。研究方法:11名健康受试者[年龄:(24.6±3.5)岁,身高:(24.6±0.10)m,质量:(65.6±14.9)kg]参与本次研究。受试者在4个动态运动条件下各进行5次实验:从0.45 m高处垂直下落至25°的斜面(IS)或平面(FS)上,使用或不使用半刚性踝关节护具,同时采集三维运动学和测力台地面反作用力数据。利用2×2(表面X踝关节护具)的重复测量方差分析来评估选定的变量。研究结果:与平面着地相比,斜面着地造成较小的垂直和内侧地面反作用力峰值。研究还发现踝关节背曲运动范围、着地角度和背曲速度、最大外翻与跖曲角速度提高,但产生了更大内翻角度和运动范围、着地内翻速度和最大跖曲力矩。踝关节护具在斜面着地时减少了达到地面反作用力第二垂直峰值的时间、着地角度、背曲速度、最大外翻和跖曲速度,但增加了跖曲力矩的最大值。研究结论:斜面增加踝关节额状面的运动范围和踝关节负荷。但是,就斜面着地而言,踝关节护具对踝关节额状面的运动范围和踝关节负荷的影响是相当有限的。 | 张松宁 Qingjian Chen Michael Wortley Divia Bhaskaran Qingjian Chen Divia Bhaskaran | 2015 | 体育科研2015，36，1： | 2 |
| 9 | Sympathetic Nerve Mediated Spinal Glia Activation Underlies Itch in a Cutaneous T-Cell Lymphoma Model显示文摘Dear Editor,Cancer-associated itch can be described as pruritus associated with malignancy.Although intractable itch associated with specific types of cancer afflicts patients badly,few effective treatments are available due to limited knowledge about the mechanisms of such itch.Globally,cancer-associated itch is an uncommon symptomin malignancy. | Fang Tong Qianru He Wan-Jie Du Huan Yang Dongping Du Shaofeng Pu Qingjian Han | 2022 | Neuroscience Bulletin2022，38，4： | 2 |
| 10 | Sorption enhancement of 1-naphthol onto a hydrophilic hyper-cross-linked polymer resin显示文摘 | Weiming Zhang Changhong Hong Bingcai Pan Qingjian Zhang Peijuan Jiang Kun Jia | 2008 | Journal of Hazardous Materials2008，，1： | 1 |
| 11 | EFFECTS OF DELAYED TREATMENT WITH TRANSFORMING GROWTH FACTOR-β SOLUBLE RECEPTOR IN A THREE-DOSE BLEOMYCIN MODEL OF LUNG FIBROSIS IN HAMSTERS显示文摘 | Qingjian Wang Dallas M. Hyde Philip J. Gotwals Shri N. Giri | 2002 | Experimental Lung Research2002，，6： | 1 |
| 12 | Rational first integrals for periodic systems 显示文摘 | JIAO Jia SHI Shaoyun ZHOU Qingjian | 2011 | Z Angew Math Phys2011，62，： | 1 |
| 13 | Does scatter-hoarding of seeds benefit cache owners or pilferers?显示文摘The scatter-hoarding behavior of granivorous rodents plays an important role in seed dispersal and seedling regeneration of trees,as well as the evolution of several well-known mutualisms between trees and rodents in forest ecosystems.Because it is difficult to identify seed hoarders and pilferers under field conditions by traditional methods,the full costs incurred and benefits accrued by scatter-hoarding have not been fully evaluated in most systems.By using infrared radiation camera tracking and seed tagging,we investigated the benefits and losses of scatter-hoarded seeds(Camellia oleifera)for 3 sympatric rodent species(Apodemus draco,Niviventer confucianus and Leopoldamys edwardsi)in a subtropical forest of Southwest China during 2013 to 2015.We established the relationships between the rodents and the seeds at the individual level.For each rodent species,we calculated the cache recovery rate of cache owners,as well as conspecific and interspecific pilferage rates.We found that all 3 sympatric rodent species had a cache recovery advantage with rates that far exceeded average pilferage rates over a 30-day tracking period.The smallest species(A.draco)showed the highest rate of scatter-hoarding and the highest recovery advantage compared with the other 2 larger species(N.confucianus and L.edwardsi).Our results suggest that scatter-hoarding benefits cache owners in food competition,supporting the pilferage avoidance hypothesis.Therefore,scatter-hoarding behavior should be favored by natural selection,and plays a significant role in species coexistence of rodent community and in the formation of mutualism between seeds and rodents in forest ecosystems. | Haifeng GU Qingjian ZHAO Zhibin ZHANG | 2017 | Integrative Zoology2017，12，6： | 1 |
| 14 | Exact moment matching model of transmission lines and application to interconnect delay estimation显示文摘 | E S Kuh | 1995 | IEEE Trans1995，3，2： | 1 |
| 15 | An Accurate Time Domain Interconnect Model of Transmission Line Networks显示文摘 | Ernest S Kuh | 1996 | IEEE Trans Circuits Syst1996，39，： | 1 |
| 16 | Moment models of general transmission lines with application to interconnect analysis and optimization显示文摘 | E S Kuh Tianxiong Xue | 1996 | IEEE Trans1996，4，4： | 1 |
| 17 | Passive Multipoint Moment Maching Model Order Reduction Algorithom on Multiport Distributed Interconnect Networks显示文摘 | Wang J M L Kuh E S | 1999 | IEEE Trans CAS-Ⅰ1999，46，1： | 1 |
| 18 | Syn-thesis of highly selective magnetic mesoporous adsorbent 显示文摘 | Chen Xinqing Lam K F Zhang Qingjian | 2009 | Journal of Physical Chemistry C2009，113，： | 1 |
| 19 | Mechanosensitive Ion Channel TMEM63A Gangs Up with Local Macrophages to Modulate Chronic Post-amputation Pain显示文摘Post-amputation pain causes great sufering to amputees,but still no efective drugs are available due to its elusive mechanisms.Our previous clinical studies found that surgical removal or radiofrequency treatment of the neuroma at the axotomized nerve stump efectively relieves the phantom pain aficting patients after amputation.This indicated an essential role of the residual nerve stump in the formation of chronic post-amputation pain(CPAP).However,the molecular mechanism by which the residual nerve stump or neuroma is involved and regulates CPAP is still a mystery.In this study,we found that nociceptors expressed the mechanosensitive ion channel TMEM63A and macrophages infltrated into the dorsal root ganglion(DRG)neurons worked synergistically to promote CPAP.Histology and qRT-PCR showed that TMEM63A was mainly expressed in mechanical pain-producing non-peptidergic nociceptors in the DRG,and the expression of TMEM63A increased signifcantly both in the neuroma from amputated patients and the DRG in a mouse model of tibial nerve transfer(TNT).Behavioral tests showed that the mechanical,heat,and cold sensitivity were not afected in the Tmem63a-/-mice in the naïve state,suggesting the basal pain was not afected.In the infammatory and post-amputation state,the mechanical allodynia but not the heat hyperalgesia or cold allodynia was signifcantly decreased in Tmem63a-/-mice.Further study showed that there was severe neuronal injury and macrophage infltration in the DRG,tibial nerve,residual stump,and the neuromalike structure of the TNT mouse model,Consistent with this,expression of the pro-infammatory cytokines TNFα,IL-6,and IL-1βall increased dramatically in the DRG.Interestingly,the deletion of Tmem63a signifcantly reduced the macrophage infltration in the DRG but not in the tibial nerve stump.Furthermore,the ablation of macrophages signifcantly reduced both the expression of Tmem63a and the mechanical allodynia in the TNT mouse model,indicating an interaction between nociceptors and macrophages,and that these two factors gang up together to regulate the formation of CPAP.This provides a new insight into the mechanisms underlying CPAP and potential drug targets its treatment. | Shaofeng Pu Yiyang Wu Fang Tong Wan-Jie Du Shuai Liu Huan Yang Chen Zhang Bin Zhou Ziyue Chen Xiaomeng Zhou Qingjian Han Dongping Du | 2023 | Neuroscience Bulletin2023，39，2： | 1 |
| 20 | Software design for water environment remote monitoring system based on mobile devices显示文摘 | Wei Qingjian Ning Jin Lou Xizhong | 2011 | Applied Mechanics and Materials:Information Technology for Manufacturing Systems Ⅱ2011，，58： | 1 |
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