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| 1 | Spatial distribution characteristics of bacterial community structure and gene abundance in sediments of the Bohai Sea显示文摘This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants. | Quanrui Chen Jingfeng Fan Jie Su Hongxia Ming Zhihao Sun Mengfei Li Xiaohui Zhao Yantao Wang Yingxue Zhang Huizhen Zhang Yuan Jin Xiaowan Ma Bin Wang | 2020 | Acta Oceanologica Sinica2020，39，2： | 2 |
| 2 | Establishment of an animal model of dural venous sinus embolism显示文摘BACKGROUND: The pathological mechanism of secondary brain lesion following an embolism remains unclear. The establishment of an animal model that imitates the clinical pathophysiological processes is crucial to better study this disease during a certain time window. OBJECTIVE: To establish a new animal model of dural venous sinus embolism that is simple, has a high success rate, and emulates the pathophysiological course of clinical disease. DESIGN, TIME AND SETTING: A randomized block design trial was performed at the Department of Anatomy, Ningxia Medical College between March and December 2007. MATERIALS: Fifty-eight healthy, adult, Sprague Dawley rats were used in the present study. Plastic emboli, with a total length of 0.4 cm, were self-made. Each plastic embolus had a conical anterior segment; the largest diameter being 0.12 cm. The posterior segment became gradually thin and flat, with a width of 0.2 cm and length of 0.1 cm. METHODS: The fifty-eight rats were randomly divided into three groups: control (n = 6), embolism (n = 26), and sham-embolism (n = 26) groups. In the embolism group, a solid embolus was slowly inserted and fixed into the posterior part of the superior sagittal sinus against the flow of blood. The posterior segment was detained outside the superior sagittal sinus for fixing. In the sham-embolism group, rats were subjected only to sinus sagittalis superior exposure. In the control group, rats received no treatments. In both the embolism and the sham-embolism groups, the rat brains were resected at 6 hours, 1, 3, and 5 days post-surgery. MAIN OUTCOME MEASURES: (1) Brain surface appearance in the embolism and sham-embolism groups. (2) Thrombosis in the embolism group. (3) Cerebrospinal fluid content in the above-mentioned two groups. RESULTS: In the embolism group, the model success rate was 92% (24/26). There was visible thrombosis in the superior sagittal sinus. Cerebral edema was noticeable under a microscope. These changes were visible at 6 hours after embolism and were most obvious at 1 day after surgery. In the embolism group, cerebrospinal fluid content reached a peak level at 6 hours post-surgery and was significantly increased compared with the sham-embolism and the control groups (P < 0.05). At 1 day post-surgery, the amount of cerebrospinal fluid was still greater in the embolism group compared to the control group (P < 0.05) CONCLUSION: The method of inserting a solid embolus has many benefits, such as being able to directly observe, short cycle time, and minimal wound. The detained posterior segment prevents movement and perfusion of embolus, which results in a high success rate. | Peixian Zhang Chongzhi Zhang Yi Qin Quanrui Ma Jianying Du Ying Cai | 2008 | Neural Regeneration Research2008，3，5： | 1 |
| 3 | Down-regulation of N-methyl-D-aspartate receptor subunits 1 affects neurogenesis of hippocampal neural stem cells显示文摘Schizophrenia is a common and serious mental illness characterized by severe impairments in thinking,emotions,and behaviors.Usually,the cognitive deficits of schizophrenia are closely associated with abnormal neurogenesis due to the hypofunction of certain neural receptors such as N-methyl-D-aspartate receptors(NMDARs),which mediates neurotransmission.However,little is known about the involvement of NMDAR1 in regulating hippocampal neurogenesis in schizophrenia.In the current study,we present evidence suggesting that NMDAR1 regulates hippocampal neurogenesis as lentivirus-mediated shRNA silencing NMDAR1 gene or blocking with MK-801 results in abnormal neurogenesis consistently found in schizophrenia.The important finding was clearly demonstrated by the multiparametric assessments,including morphology,immunofluorescence,western blotting,and flow cytometry.Simultaneously,our results indicated that knockdown and blockade of NMDAR1 significantly attenuated the proliferation of hippocampal neural stem cells(hNSCs)and decreased the differentiation to neurons.More importantly,the blockade of NMDAR1 with MK-801 aggravated the apoptosis of hNSCs.Thus,it is likely that NMDAR1 functions as a new target for the treatment of schizophrenia.Our present study may provide a novel insight for further investigation of the pathogenesis of schizophrenia. | YUQING HE LI GUO JUAN DING HAOWEN LV QUANRUI MA CHEN LI YU SHAO QIANG LIU CHUN ZHANG HAO YANG JUAN LIU | 2021 | BIOCELL2021，45，2： | 0 |
| 4 | Effect of Sox10 on remyelination of the hippocampus in cuprizone-induced demyelinated mice显示文摘This study aimed to analyze the regulatory role of Sox10 in remyelination of the hippocampus in a demyelination mouse model.Mice were fed with 0.2%cuprizone(CPZ)for six weeks to.establish the acute demyelinating model.Behavioral changes of these mice were assessed via open field and tail suspension tests.The ultrastructure of the myelin sheaths in the hippocampus was observed by transmission electron microscopy.The expression levels of myelin sheath-related proteins and the transcription factor Sox10 were detected with immunohistochemistry and Western blotting.Furthermore,Soxl0-overexpressing adeno-associated viras was injected into the hippocampus after esta blishing the demyelinating model to investigate effects of Sox10 on remyelination.CPZ mice showed abnormal behavioral changes,a large number of pathological changes in the myelin sheaths,and significantly reduced protein expression of the myelin sheath markers.The protein expression of(the oligodendrocyte precursor cell marker)NG2 increased,whereas Sox10 expression decreased.After Sox10 overexpression,the abnormal behavior was improved,the ultrastructure of the myelin sheaths was restored,and the expression of myelin sheath protein was reversed. | Shao Yu Zhang Yiwei Ma Quanrui Liu Juan | 2021 | 解剖学杂志2021，44，S01： | 0 |
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