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| 1 | Assessment on alien herbicide-resistant gene flow among crucifers by sexual compatibility显示文摘Cross-pollination and high cross-compatibility are frequently found in many cruciferous plants, which im-plies that there might be potential risk of alien gene flow among crucifers. In this work, the alien glyphosate-resistant gene flow between GM (Genetically modified) rapeseed vari-ety Q3 (Brassica napus) and 6 varieties from 5 cruciferous species was assessed by sexual compatibility through aniline blue fluorescence observation, manual cross, mentor pollina-tion with the mixture pollen and DNA identification. The results indicated that the compatibility indices of two B. napus varieties, B. juncea var. multiceps Tsen et Lee and Chinese cabbage (B. chinensis) (as female) with Q3 (as male) were 16.15, 12.77, 2.345 and 0.85, respectively. F1 plants were examined by PCR and all of these samples were confirmed as positive. Crossing between Orychophragmus violaceus, Des-curainia sophia (as female) and Q3 (as male) expressed high cross-incompatibility. Fluorescence microscope observation indicated that growing of pollen tube terminated at the upper 1/3 part of the style of O. violaceus and on the stigma surface of D. sophia. The mentor plants were further analyzed by PCR and all were shown to be negative. Under the neighboring growth and natural pollination conditions, the rates of gene flow to two B. napus varieties were 2.3278% and 2.1487%, respectively, B. juncea var. multiceps Tsen et Lee and Chinese cabbage were 1.0157% and 0.9236%, re-spectively. No gene flow was observed in O. violaceus and D. sophia. Frequency of gene flow was highly correlated with sexual compatibility. Due to possible alien gene flow among crucifers, special consideration and care should be taken to grow GM rapeseed. | ZHAO Xiangxiang LU Weiping QI Cunkou PU Huiming XIA Qiuxia LU Dalei LIU Geshan WANG Youping | 2005 | Chinese Science Bulletin2005，50，15： | 6 |
| 2 | Gene flow from genetically modified herbicide-resistant rapeseed to cruciferous weeds显示文摘性相容性在之间遗传上修改了(美国通用汽车公司)glyphosate 抵抗的油菜籽变化 Q3 (Brassica 马来麝 L.) 和 5 棵饰有十字架的杂草通过荧光显微镜学的观察被学习并且在用手的授粉以后跨富饶。结果显示了那 Q3 (同样男性) 与 Thlaspi arvense L. 高度不兼容, Capsella bursa-pastoris (L.) 苜蓿, Cardamine hirsuta L。并且 Rorippa palustris (L.) Besser (作为女性) 。荧光显微镜的观察显示出那成长在耻辱表面上或在风格的上面的 1/3 部分终止的花粉试管。然而, B。juncea x Q3 是兼容的,并且相容性索引是 1.65。在附近的生长和自然授粉条件下面,基因的率从 Q3 流动到 T。arvense, C。bursa-pastoris, C。多毛并且 R。palustris 都是 0,当它为 B 是 0.86% 时。juncea。这些结果显示处于在美国通用汽车公司油菜籽之间的基因流动的率有差别,基因流动的饰有十字架的野杂草。 | ZHAO Xiangxiang XIA Oiuxia LU Dalei LU Weiping QI Cunkou PU Huiming LIU Geshan ZHAO Jian WANG Youping | 2006 | Progress in Natural Science:Materials International2006，16，9： | 3 |
| 3 | Ethylene response factor BnERF2-like (ERF2.4) from Brassica napus L.enhances submergence tolerance and alleviates oxidative damage caused by submergence in Arabidopsis thaliana显示文摘Ethylene response factor proteins play an important role in regulating a variety of stress responses in plants,but their exact functions in submergence stress are not well understood.In this study,we isolated BnE RF2.4 from Brassica napus L.to study its function in submergence tolerance.The expression of the BnE RF2.4 gene in B.napus and the expression of antioxidant enzyme genes in transgenic Arabidopsis were analyzed by quantitative RT-PCR.The expression of BnE RF2.4 was induced by submergence in B.napus and the overexpression of BnE RF2.4 in Arabidopsis increased the level of tolerance to submergence and oxidative stress.A histochemical method detected lower levels of H_2O_2,O^(·-)_2and malondialdehyde(MDA) in transgenic Arabidopsis.Compared to the wild type,transgenic lines also had higher soluble sugar content and higher activity of antioxidant enzymes,which helped to protect plants against the oxidative damage caused by submergence.It was concluded that BnE RF2.4 increased the tolerance of plants to submergence stress and may be involved in regulating soluble sugar content and the antioxidant system in defense against submergence stress. | Yanyan Lv Sanxiong Fu Song Chen Wei Zhang Cunkou Qi | 2016 | The Crop Journal2016，4，3： | 2 |
| 4 | Cloning of CRABS CLAW Gene from Brassica napus and Construction of Its RNA Interference Expression Vector显示文摘CRABS CLAW( CRC) is a member of the YABBYA transcription factor gene family that plays an important role in floral organ development of plants.This study aimed to further investigate the regulatory function of CRC transcription factor in the development of floral organs of rape( Brassica napus L.). A 580 bp fragment of CRC gene was cloned by RT-PCR from total RNA of buds of rape cultivar Ningyou No. 10 to construct an inverted repeated expression cassette of CRC gene using intermediate vector p Hurricane. Firstly,CRC gene fragment was positively inserted into the 5'end of a spliceable intron and negatively inserted into the3' end of the intron. Subsequently,Ca MV35 S promoter sequence and inverted repeated expression cassette of CRC gene were transferred into p UC18 multiple clone site of binary expression vector p CAMBIAI1390. The constructed interference expression vector was named p A6-CRCi,which was further confirmed by restriction enzyme digestion and sequencing. | Xiaoying ZHOU Sanxiong FU Song CHEN Chao ZHANG Cunkou QI | 2016 | Agricultural Biotechnology2016，5，1： | 0 |
| 5 | Screening and Identification of Waterlogging Tolerance in Brassica napus Germplasm Resources显示文摘In order to evaluate,screen and identify waterlogging-tolerant Brassica napus resources and provide good germplasm materials for breeding of waterlogging-tolerant rape,608 B. napus germplasm materials from different sources were identified and screened for waterlogging-tolerant germplasms. The identification results showed that tested materials had dead seedling rates in the range of 0-100%,indicating that these rape germplasm materials varied extensively in waterlogging tolerance. Among the 608 materials,waterlogging-tolerant materials( grade I,relative dead seedling rate≤21%) accounted for 25. 49%; non-tolerant materials( grade Ⅳ + V,dead seedling rate≥61%) accounted for 29. 43%; and other materials had waterlogging tolerance between the two. The identification results of waterlogging tolerance in the 349 rape germplasm resource from the upper,middle and lower reaches of Yangtze River and Huang-Huai Basin showed that materials from the lower Yangtze River had the lowest average dead seedling rate of 38. 8%,which was remarkably lower than materials from other ecological regions,and waterlogging-tolerant materials among them accounted for 33. 6%,which was also remarkably higher than materials from other ecological regions. It could thus be seen that materials from the lower Yangtze River have stronger waterlogging tolerance than those from other ecological regions overall. In this study,24 highly-tolerant B. napus germplasms( dead seedling rate < 1%) were selected from the 608 B. napus germplasm materials,which could be used for breeding of waterlogging-tolerant rape and related fundamental research. | Yun LI Cunkou QI Sanxiong FU Feng CHEN Song CHEN | 2019 | Agricultural Biotechnology2019，8，1： | 0 |
| 6 | Gene flow from transgenic oilseed rape (Brassica napus L.) to cruciferous weeds under mentor pollen inducement显示文摘The alien gene flow between genetically modified glyphosate-resistant rapeseed variety Q3 (Brassica napus L.) and four cruciferous weeds was studied under mentor pollen inducement. The results showed that when Thlaspi arvense L., Capsella bursa-pastoris (L.) Medic, Cardamine hirsuta L. and Rorippa palustris (L.) Besser were pollinated with mentor pollen, the mixed Q3 and the weed, pollen grains aggregated largely and germinated quickly, and the numbers of pollen tubes penetrating into the style and the ovary were greatly increased as compared with corresponding self-pollination groups. Twenty four to forty eight hours after pollination, several pollen tubes were observed to penetrate into the ovule via micropyle in each mentor combination. However, when the mentor progenies were analyzed by PCR, all of them showed negative for the Q3 herbicide-resistant gene. Collectively, these results indicated that crossing between T. arvense, C. bursa-pastoris, C. hirsuta, R. palustris (as female) and Q3 (as male) was highly incompatible and the herbicide-resistant gene could not flow from Q3 to these four weeds. | Zhao Xiangxiang Luo Yuming Lu Weiping Qi Cunkou Pu Huiming Wang Youping | 2007 | Progress in Natural Science:Materials International2007，17，11： | 0 |
| 7 | Tissue-specific Expression of Acetolactate Synthase (ALS), Male Sterility-inducing Effect of Tribenuron-methyl and Its Effect on ALS Activity in Brassica napus L.显示文摘[Objectives]This study was conducted to provide a basis for the rapid identification of the drug spraying effect in early stage and the molecular mechanism of chemical hybridizing in Brassica napus L.[Methods]Quantitative RT-PCR analysis showed that ALS was constitutively expressed in various tissues of 096030,including flower buds,four floral organs (calyxes,petals,stamens and pistils),roots,stems and leaves.ALS was prominently expressed in leaves and was expressed weakly in the petals and stamens.The male sterility-inducing effects of tribenuron-methyl on such two Brassica napus L.varieties as Ningyou18 and 096030 were investigated.[Results]Plants were twice sprayed with 0.2 μg/ml tribenuron-methyl on leaves.The results showed that 8-10 ml of tribenuron- methyl was applied per plant for the first time at bolting stage with 1-2 mm flower buds on 15-20 cm inflorescence,and the second spray was performed with 8-10 ml of tribenuron-methyl per plant 10 d later.The results showed that the percentage of the full sterile plants reached 100%,which lasted for the whole flowering period,and the relative seed setting rate was only about 4%.Thus,this method could fullfill the requirement of hybrid seed production in field.The in-vivo enzyme activity of acetolactate synthase (ALS) was assayed using 2 mm buds collected 3 d after spray.The results showed that 0.2 μg/ml tribenuron-methyl inhibited ALS activity.The ALS activity of Ningyou 18 (CK) and Ningyou 18 (0.2 μg/ml) was 3.20 and 1.30 μmol/(mg·h),respectively,and the ALS activity of 096030 (CK) and 096030 (0.2 μg/ml) was 3.37 and 1.25 μmol/(mg·h),respectively.The relative enzyme activity of ALS in Ningyou18 and 096030 was 40.63% and 37.23%,respectively,both of which decreased significantly.[Conclusions]These results showed that the change of ALS activity may be used as an index for quickly identifying and predicting the chemical hybridizing effect of tribenuron-methyl. | Sanxiong FU Xiaoying ZHOU Cunkou QI | 2019 | Agricultural Biotechnology2019，8，3： | 0 |
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