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| 1 | Diallyl disulfide-induced G2/M arrest of human gastric cancer MGC803 cells involves activation of p38 MAP kinase pathways显示文摘AIM: To determine the role of p38 MAP kinase signal transduction pathways in diallyl disulfide (DADS)-induced G2/M arrest in human gastric cancer MGC803 cells.METHODS: MGC803 cell growth inhibition was measured by MTT assay. Phase distribution of cell cyde was analyzed by flow cytometry. Expression of Cdc25C, p38, phosphorylation of p38 (pp38) were determined by Western blotting.RESULTS: MT1- assay showed that SB203580, a specific p38 MAPK inhibitor blocked DADS-induced growth inhibition.Flow cytometry analysis revealed that treatment of MGC803 cells with 30 mg/L DADS increased the percentage of cells in the G2/M phase from 9.3% to 39.4% (P<0.05), whereas inhibition of p38 activity by SB203580 abolished induction of G2/M arrest by DADS. Western blotting showed that phosphorylation of p38 was increased 3.52-fold following treatment of MGC803 cells with 30 mg/L DADS for 20 min(P<0.05), whereas Cdc25C was decreased 68% following treatment of MGCS03 cells with 30 mg/L DADS for 24 h(P<0.05). Decreased Cdc25C protein expression by DADS was attenuated by SB203580 (P<0.05).CONCLUSION: DADS-induced G2/M arrest of MGC803 cells involves activation of p38 MAP kinase pathways.Decreased Cdc25C protein expression by p38 MAPK played a crucial role in G2/M arrest after treatment with DADS. | Jing-PingYuan Gui-HuaWang HuiLing QiSu Yue-HongYang YingSong Rong-JunTang YaoLiu ChenHuang | 2004 | World Journal of Gastroenterology2004，10，18： | 25 |
| 2 | Expression of p16 gene and Rb protein in gastric carcinoma and their clinicopathological significance显示文摘AIM: To analyze the correlation between the protein expression of p16 and Rb genes in gastric carcinoma (GC),to investigate the role of p16 gene in invasion and lymph node metastasis of GC, and to examine the deletion and mutation in exon 2 of p16 gene in GC.METHODS: The protein expression of p16 and Rb genes was examined by streptavidin-peroxidase conjugated method (S-P) in normal gastric mucosa, dysplastic gastric mucosa and GC. The deletion and mutation of p16 gene were examined by polymerase chain reaction (PCR) and polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) respectively in normal gastric mucosa and GC.RESULTS: The positive rates of P16 and Rb protein expression respectively were 96% (77/80) and 99%(79/80) in normal gastric mucosa, 92% (45/50) and 80%(40/50) in dysplastic gastric mucosa, 48% (58/122) and 60% (73/122) in GC. The positive rates of P16 and Rb protein expression in GC were significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P<0.05). The positive rate of P16 protein expression in mucoid carcinoma (10%, 1/10) was significantly lower than that in poorly differentiated carcinoma (51%, 21/41),undifferentiated carcinoma (58%, 15/26) and signet ring cell carcinoma (62%, 10/16) (P<0.05). The positive rates of P16 protein in 30 cases of paired primary and lymph node metastatic GC were 47% (14/30) and 17% (5/30)respectively, being significantly lower in the later than in the former (P<0.05). There was no mutation in exon 2 of p16 gene in the 25 freshly resected primary GCs. But five cases in the 25 freshly resected primary GCs displayed deletion in exon 2 of p16 gene. The positive rate of both P16 and Rb proteins was 16% (14/90), and the negative rate of both P16 and Rb proteins was 8% (7/90) in 90GCs. The rate of positive P16 protein with negative Rb protein was 33% (30/90). The rate of negative P16 protein with positive Rb protein was 43% (39/90). There was reverse correlation between P16 and Rb expression in 90GCs (P<0.05).CONCLUSION; The loss protein expression of p16 and Rb genes is related to GC. The loss expression of P16protein is related to the histopathologic subtypes and lymph node metastasis of GC. Expression of P16 and Rb proteins in GC is reversely correlated. The deletion but not mutation in exon 2 of p16 gene may be involved in GC. | Xiu-ShengHe Ying-HuiRong QiSu QiaoLuo Dong-MeiHe Yan-LanLi YanChen | 2005 | World Journal of Gastroenterology2005，11，15： | 14 |
| 3 | Expression of p21^(WAF1) and p53 and polymorphism of p21^(WAF1) gene in gastric carcinoma显示文摘To investigate the relationship between expression of p21WAF1 and p53 gene, and to evaluate the deletion and polymorphism of p21WAF1 gene in gastric carcinoma (GC). | Hai-LongXie Qisu Xiu-ShengHe xiao-QiuLiang Jian-GuoZhou YinSong Yi-QinLi | 2004 | World Journal of Gastroenterology2004，10，8： | 12 |
| 4 | Analytic solutions of simple flows and analysis of nonslip boundary conditions for the lattice Boltzmann BGK model显示文摘 | Xiaoyi He Qisu Zou Li-Shi Luo Micah Dembo | 1997 | Journal of Statistical Physics (-)1997，，1： | 1 |
| 5 | Analytic Solutions ofSimple Flows and Analysis of Nonslip Boundary Conditions forthe Lattice Boltzmann BGK Model显示文摘 | He Xiaoyi Zou Qisu Luo Lishi | 1997 | J Stat Phys1997，87，12： | 1 |
| 6 | Material point method applied to multiphase flows显示文摘 | Duan Z Zhang Qisu Z | | 0，，： | 1 |
| 7 | Simulation of cavity flow by the lattice Boltzmann method 显示文摘 | Hou Shuling Zou Qisu Chen Shiyi | 1995 | Journal of Computational Physics1995，118，2： | 1 |
| 8 | Evaluation of Two Lattice Boltzmann Models for Multiphase Flows显示文摘 | Shuling Hou Xiaowen Shan Qisu Zou Gary D. Doolen Wendy E. Soll | 1997 | Journal of Computational Physics1997，，2： | 1 |
| 9 | On pressure and velocity boundary conditions for the lattice Boltzmann BGK mode 显示文摘 | Zou Qisu He Xiaoyi | 1997 | Physics of Fluids1997，9，6： | 1 |
| 10 | On pressure and velocity boundary conditions for the lattice Boltzmann BGK model 显示文摘 | Zou Qisu He Xiaoyi | 1997 | Physics of Fluids1997，9，6： | 1 |
| 11 | On pressure and velocity boundaryconditions for the lattice Boltzmann BGK model显示文摘 | Zou Qisu He Xiaoyi | 1997 | Physicsof Fluids1997，9，6： | 1 |
| 12 | Material point method applied to multiphase flows显示文摘 | Duan Z Zhang Qisu Z | 2008 | J Comput Phys2008，227，： | 1 |
| 13 | Analytic solutions of simple flows and analysis of nonslip boundary conditions for the lattice Boltzmann BGK model显示文摘 | Xiaoyi He Qisu Zou Li-Shi Luo Micah Dembo | 1997 | Journal of Statistical Physics (-)1997，，1： | 1 |
| 14 | On pressure and velocity boundary conditions for the lattice Boltzmann BGK model显示文摘 | Zou Qisu He Xiaoyi | 1997 | Phys Fluids1997，9，6： | 1 |
| 15 | On pressure and velocity boundary conditions for the lattice Boltzmann 13GK model显示文摘 | Zou Qisu tte Xiao | 1997 | Physics of Fluids1997，9，6： | 1 |
| 16 | On pressure and velocity bounda- ry conditions for the lattice Boltzmann BGK model显示文摘 | Zou Qisu He Xiaoyi | 1997 | Physics of Fluids1997，9，： | 1 |
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