|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Wx^lv、the Ancestral Allele of Rice Waxy Gene显示文摘In rice grains,the Waxy (Wx) gene is responsible for the synthesis of amylose,the most important determinant for eating and cooking quality.The effects of several Wx alleles on amylose content and the taste of cooked rice have been elucidated.However,the relationship between artificial selection and the evolution of various Wx alleles as well as their distribution remain unclear.Here we report the identification of an ancestral allele,Wx^lv,which dramatically affects the mouthfeel of rice grains by modulating the size of amylose molecules.We demonstrated that WF originated directly from wild rice,and the three major Wx alleles in cultivated rice (Wx^b,Wx^a,and Wx^in) differentiated after the substitution of one base pair at the functional sites.These data indicate that the Wx^lv allele played an important role in artificial selection and domestication.The findings also shed light on the evolution of various Wx alleles,which have greatly contributed to improving the eating and cooking quality of rice. | Changquan Zhang Jihui Zhu Shengjie Chen Xiaolei Fan Qianfeng Li Yan Lu Min Wang Hengxiu Yu Chuandeng Yi Shuzhu Tang Minghong Gu Qiaoquan Liu | 2019 | Molecular Plant2019，12，8： | 22 |
| 2 | A rare Waxy allele coordinately improves rice eating and cooking quality and grain transparency显示文摘In rice(Oryza sativa), amylose content(AC) is the major factor that determines eating and cooking quality(ECQ). The diversity in AC is largely attributed to natural allelic variation at the Waxy(Wx)locus. Here we identified a rare Wx allele, Wx^(mw) ,which combines a favorable AC, improved ECQ and grain transparency. Based on a phylogenetic analysis of Wx genomic sequences from 370 rice accessions, we speculated that Wx^(mw) may have derived from recombination between two important natural Wx alleles, Wx^(in) and Wx^(b). We validated the effects of Wx^(mw) on rice grain quality using both transgenic lines and near-isogenic lines(NILs). When introgressed into the japonica Nipponbare(NIP) background, Wx^(mw) resulted in a moderate AC that was intermediate between that of NILs carrying the Wx^(b)allele and NILs with the Wx^(mp) allele. Notably, mature grains of NILs fixed for Wx^(mw) had an improved transparent endosperm relative to soft rice. Further, we introduced Wx^(mw) into a high-yielding japonica cultivar via molecular marker-assisted selection: the introgressed lines exhibited clear improvements in ECQ and endosperm transparency. Our results suggest that Wx^(mw) is a promising allele to improve grain quality, especially ECQ and grain transparency of high-yielding japonica cultivars, in rice breeding programs. | Changquan Zhang Yong Yang Shengjie Chen Xueju Liu Jihui Zhu Lihui Zhou Yan Lu Qianfeng Li Xiaolei Fan Shuzhu Tang Minghong Gu Qiaoquan Liu | 2021 | Journal of Integrative Plant Biology2021，63，5： | 18 |
| 3 | Entropy Generation Analysis in a Mixed-Flow Compressor with Casing Treatment显示文摘Casing treatments(CT) can effectively extend compressors flow ranges with the expense of efficiency penalty. Compressor efficiency is closely linked to loss. Only revealing the mechanisms of loss generation can design a CT with high aerodynamic performance. In the paper, a highly-loaded mixed-flow compressor with tip clearance of 0.4 mm was numerically studied at a rotational speed of 30,000 r/min to reveal the effects of axial slot casing treatment(ASCT) on the loss mechanisms in the compressor. The results showed that both isentropic efficiency and stall margin were improved significantly by the ASCT. The local entropy generation method was used to analyze the loss mechanisms and to quantify the loss distributions in the blade passage. Based on the axial distributions of entropy generation rate, for both the cases with and without ASCT, the peak entropy generation rate increased in the rotor domain and decreased in the stator domain during throttling the compressor. The peak entropy generation in rotor was mainly caused by the tip leakage flow and flow separations near the rotor leading edge for the mixed-flow compressor no matter which casing was applied. The radial distributions of entropy generation rate showed that the reduction of loss in the rotor domain from 0.4 span to the rotor casing was the major reason for the efficiency improved by ASCT. The addition of ASCT exerted two opposite effects on the losses generated in the compressor. On the one hand, the intensity of tip leakage flow was weakened by the suction effect of slots, which alleviated the mixing effect between the tip leakage flow and main flow, and thus reduced the flow losses;On the other hand, the extra losses upstream the rotor leading edge were produced due to the shear effect and to the heat transfer. The aforementioned shear effect was caused by the different velocity magnitudes and directions, and the heat transfer was caused by temperature gradient between the injected flow and the incoming flow. For case with smooth casing(SC), 61.61% of the overall loss arose from tip leakage flow and casing boundary layer. When the ASCT was applied, that decreased to 55.34%. The loss generated by tip leakage flow and casing boundary layer decreased 20.54% relatively by ASCT. | ZHANG Qianfeng DU Juan LI Zhihui LI Jichao ZHANG Hongwu | 2019 | Journal of Thermal Science2019，28，5： | 5 |
| 4 | Overexpressed Cyclin D1 and CDK4 proteins are responsible for the resistance to CDK4/6 inhibitor in breast cancer that can be reversed by PI3K/mTOR inhibitors显示文摘CDK4/6 inhibitors are the standard treatment in advanced HR+/HER2-breast cancer patients.Nevertheless,the resistance to CDK4/6 inhibitors is inevitable and the strategies to overcome resistance are of great interest.Here,we show that the palbociclibresistant breast cancer cells expressed significantly higher levels of Cyclin D1 and CDK4 proteins because of upregulated protein synthesis.Silencing Cyclin D1 or CDK4 led to cell cycle arrest while silencing Cyclin E1 or CDK2 restored the sensitivity to palbociclib.Furthermore,PI3K/mTOR pathway was hyper-activated in palbociclib-resistant cells,leading to more phosphorylated 4E-BP1 and higher levels of Cyclin D1 and CDK4 translation.Targeting PI3K/mTOR pathway with a specific PI3Kαinhibitor(BYL719)or an mTOR inhibitor(everolimus)reduced the protein levels of Cyclin D1 and CDK4,and restored the sensitivity to palbociclib.The tumor samples expressed significantly higher levels of Cyclin D1,CDK4,p-AKT and p-4E-BP1 after progression on palbociclib treatment.In conclusion,our findings suggest that overexpressed Cyclin D1 and CDK4 proteins lead to the resistance to CDK4/6 inhibitor and PI3K/mTOR inhibitors are able to restore the sensitivity to CDK4/6 inhibitors,which provides the biomarker and rationale for the combinational use of CDK4/6 inhibitors and PI3K/mTOR inhibitors after CDK4/6 inhibitor resistance in breast cancer. | Zijie Cai Jingru Wang Yudong Li Qianfeng Shi Liang Jin Shunying Li Mengdi Zhu Qi Wang Lok Lam Wong Wang Yang Hongna Lai Chang Gong Yandan Yao Yujie Liu Jun Zhang Herui Yao Qiang Liu | 2023 | Science China(Life Sciences)2023，66，1： | 4 |
| 5 | Composition design of superhigh strength maraging stainless steels using a cluster model显示文摘The composition characteristics of maraging stainless steels were studied in the present work investigation using a cluster-plus-glue-atom model. The least solubility limit of high-temperature austenite to form martensite in basic Fe–Ni–Cr corresponds to the cluster formula [NiFe12]Cr3,where NiFe12is a cuboctahedron centered by Ni and surrounded by 12 Fe atoms in FCC structure and Cr serves as glue atoms. A cluster formula [NiFe12](Cr2Ni) with surplus Ni was then determined to ensure the second phase(Ni3M) precipitation,based on which new multicomponent alloys [(Ni,Cu)16Fe192](Cr32(Ni,Mo,Ti,Nb,Al,V)16) were designed. These alloys were prepared by copper mould suction casting method,then solid-solution treated at 1273 K for 1 h followed by water-quenching,and finally aged at 783 K for 3 h. The experimental results showed that the multi-element alloying results in Ni3M precipitation on the martensite,which enhances the strengths of alloys sharply after ageing treatment. Among them,the aged [(Cu4Ni12)Fe192](Cr32(Ni8.5Mo2Ti2Nb0.5Al1V1)) alloy(Fe74.91Ni8.82Cr11.62Mo1.34Ti0.67Nb0.32Al0.19V0.36Cu1.78wt%) has higher tensile strengths with YS?1456 MPa and UTS?1494 MPa. It also exhibits good corrosion-resistance in 3.5 wt% NaCl solution. | Zhen Li Ruiqian Zhang Qianfeng Zha Yingmin Wang Jianbing Qiang Chuang Dong | 2014 | Progress in Natural Science:Materials International2014，24，1： | 4 |
| 6 | Starch biosynthesis in cereal endosperms: An updated review over the last decade显示文摘Starch is a vital energy source for living organisms and is a key raw material and additive in the food and non-food industries.Starch has received continuous attention in multiple research fields.The endosperm of cereals(e.g.,rice,corn,wheat,and barley)is the most important site for the synthesis of storage starch.Around 2010,several excellent reviews summarized key progress in various fields of starch research,serving as important references for subsequent research.In the past 10 years,many achievements have been made in the study of starch synthesis and regulation in cereals.The present review provides an update on research progress in starch synthesis of cereal endosperms over the past decade,focusing on new enzymes and non-enzymatic proteins involved in starch synthesis,regulatory networks of starch synthesis,and the use of elite alleles of starch synthesis-related genes in cereal breeding programs.We also provide perspectives on future research directions that will further our understanding of cereal starch biosynthesis and regulation to support the rational design of ideal quality grain. | Lichun Huang Hongyan Tan Changquan Zhang Qianfeng Li Qiaoquan Liu | 2021 | Plant Communications2021，2，5： | 4 |
| 7 | Dual stability enhancement mechanisms of axial-slot casing treatment in a high-speed mixed-flow compressor with various tip clearances显示文摘The influence of Axial-Slot Casing Treatment(ASCT)on the performance and stability enhancement mechanisms of ASCT were experimentally and numerically investigated in a highspeed mixed-flow compressor under three different tip clearances.Unsteady simulations showed the compressor stalled through end-wall stall route,i.e.the spike stall inception originating from rotor tip region,which was validated by dynamical measurements.When the ASCT was applied,greater than 20%of Stall Margin Improvement(SMI)could be achieved for the compressor under each tip clearance size.The streamwise velocity contours and flow structures in the tip region and axial slots were deeply analyzed to explore how the so called‘‘suction and injection effects'generated by the ASCT manipulate tip clearance flow and enhance the stability of compressor under different tip clearances.It was found that the dominant stability enhancement mechanisms of ASCT varies with tip clearance size for the mixed-flow compressor.(A)For the small tip clearance,the dominant mechanism of stability enhancement is the blockage reduction generated in the blade passage by the suction effect of ASCT.(B)For the large tip clearance,the injection effect of the ASCT is the dominant mechanism of stability enhancement with ASCT,which plays the leading role in delaying the spillage of incoming/tip leakage flow interface at the rotor Leading Edge(LE)plane. | Qianfeng ZHANG Juan DU Jichao LI Ming ZHAO Hongwu ZHANG | 2021 | Chinese Journal of Aeronautics2021，34，4： | 2 |
| 8 | LIN9 confers paclitaxel resistance in triple negative breast cancer cells by upregulating CCSAP显示文摘LIN9 functions to regulate cell mitotic process.Dysregulation of LIN9 expression is associated with development of human cancers.In this study we assessed the association of LIN9 expression with paclitaxel resistance and clarified the underlying mechanisms for the first time.LIN9 expression in breast cancer tissues was retrieved from publicly available online databases and statistically analyzed.Human TNBC cell lines MDA-MB-231 and MDA-MB-468 and their corresponding paclitaxelresistant sublines 231PTX and 468PTX were used to assess the expression of LIN9 by qRT-PCR and Western blot,cell growth by cell counting,cell viability by MTS assay,and cell apoptosis by flow cytometry.The data showed that high LIN9 expression in breast cancer patients receiving chemotherapy was related to poor overall survival(OS).LIN9 expression was upregulated in paclitaxel-resistant TNBC cells compared to their parental cells.Knockdown of LIN9 or treatment of paclitaxel-resistant TNBC cells with a bromo-and extra-terminal domain inhibitor(BETi)JQ1 which also decreased LIN9 expression enhanced the sensitivity of paclitaxel-resistant TNBC cells to paclitaxel.Mechanistically,decreased LIN9 in resistant cell lines reduced tumor cell viability,promoted multinucleated cells formation and induced tumor cell apoptosis,potentially by directly regulating microtubule-binding protein CCSAP.In conclusion,high LIN9 expression contributed to poor clinical outcomes and paclitaxel resistance in TNBC and BETi,targeting LIN9 expression,could be a reversible drug for PTX-resistant TNBC patients. | Hongna Lai Rui Wang Shunying Li Qianfeng Shi Zijie Cai Yudong Li Yujie Liu | 2020 | Science China(Life Sciences)2020，63，3： | 2 |
| 9 | Dendron-polymer hybrid mediated anticancer drug delivery for suppression of mammary cancer显示文摘Dendron-polymer-based nanoscale and stimuli-responsive drug delivery systems have shown great promise in tumor-targeting accumulation without significant toxicity.Here we report a dendronized polymer-doxorubicin(DOX)hybrid(DPDH)with an improved in vivo drug delivery efficiency for cancer therapy compared with a linear polymer-DOX conjugate(LPDC).The in vitro drug release profile of DOX indicates that DPDH displays pH-responsive drug release due to cleavage of hydrazone bonds since a greater amount of DOX is released at pH 5.2 at a faster rate than at pH 7.4.DPDH efficiently enters 4 T1 cells and releases DOX to induce cytotoxicity and apoptosis.Owing to the dendronzied structure,DPDH has a significantly longer blood circulation time than LPDC.DPDH substantially enhances the therapeutic efficacy to suppress tumor growth in a 4 T1 mammary cancer model than LPDC as well as free drug,evidenced from tumor growth inhibition,TUNEL assessment and histological analysis.Biosafety of DPDH is also confirmed from hemolysis,body weight shifts during treatment and pathological analysis.This study demonstrates the use of dendronized polymer-DOX hybrids for specific drug molecules is a promising approach for drug delivery. | Dayi Pan Xiuli Zheng Miao Chen Qianfeng Zhang Zhiqian Li Zhenyu Duan Qiyong Gong Zhongwei Gu Hu Zhang Kui Luo | 2021 | Journal of Materials Science & Technology2021，，4： | 2 |
| 10 | The First-principles Study on the Occupation Behavior and the Ductility Mechanism of Zr in Ni-Ni_3Al System with Lattice Misfit显示文摘The influence of lattice misfit on the occupation behavior and the ductility effect of Zr in Ni—Ni_3AI alloys were explored.It is found in energy analysis that the preferable site of Zr between Ni sublattice and Al sublattice will change under different lattice misfit,however,the Zr prefers to segregate Ni phase rather than Ni_3AI phase in all lattice misfit range,which makes it impossible for Zr to go into Ni_3AI phase to occupy Al sublattice in Ni—Ni_3AI system.Bond order(BO)analysis shows that the localized ductility effect of Zr differs in different region,and the comparison between Zr-free and Zr-doped BO analysis successfully explain the mechanism of the embrittlement of Ni—Ni_3AI alloys and the ductility effect of Zr. | Yuxi Wu Wanglin Zhang Jia Guo Jieshan Hou Xiuyan Li Renzhong Huang Xiufang Ma Qianfeng Zhang | 2014 | Journal of Materials Science & Technology2014，30，5： | 2 |
| 11 | Generation of selectable marker-free soft transgenic rice with transparent kernels by downregulation of SSSⅡ-2显示文摘The amylose content(AC)of rice endosperm starch varies from 0 to 35%,and is associated with rice cooking and eating quality.Soft rice has low AC,generally between 6%and 15%,and its eating quality is high whether it is consumed hot or cold.However,the appearance quality of current soft rice cultivars needs to be improved,especially opaque endosperm.Conventional genetic engineering has improved some agronomic traits of soft rice varieties,but not endosperm appearance.In the present study,a RNAi construct of the soluble starch synthase II-2(SSSII-2)and the hygromycin phosphotransferase(HPT)gene were introduced into an elite japonica rice variety,Kangtiaowuyunjing(KWY8)by co-transformation.Several selectable marker-free(SMF)transgenic lines were obtained,and SSSII-2 expression was significantly downregulated in selected transgenic lines,resulting in lower AC of the endosperm.The physicochemical properties of the transgenic rice kernels,including gel consistency(GC)and rapid visco analyzer(RVA)profile,differed significantly from those of wild-type rice and were similar to those of a soft rice variety,Nanjing 46(NJ46).These findings indicate that the cooking,eating,and processing qualities of transgenic rice are comparable to those of NJ46.However,the transgenic rice endosperm retained a transparent appearance under low-moisture conditions.Thus,SMF SSSII-2 RNAi rice provides a resource for breeding soft rice with transparent endosperm. | Zhihao Xu Meimei Yu Youwei Yin Cenwen Zhu Wen Ji Changquan Zhang Qianfeng Li Honggen Zhang Shuzu Tang Hengxiu Yu Qiaoquan Liu | 2020 | The Crop Journal2020，8，1： | 2 |
| 12 | OsbZIP09,a Unique OsbZIP Transcription Factor of Rice,Promotes Rather Than Suppresses Seed Germination by Attenuating Abscisic Acid Pathway显示文摘We successfully identified a novel and unique OsbZIP transcription factor,OsbZIP09,whose mutants exhibited longer seeds and less severe pre-harvest sprouting than the wild type,but shared similar germination rate as the wild type under normal germination conditions.The expression of OsbZIP09 was induced by abscisic acid(ABA)and declined as the germination process.As a nucleus-localized transcription factor,the conserved binding motif of OsbZIP09 was identified via DNA affinity purification sequencing technique.Further evidences indicated that OsbZIP09 directly enhanced the expression of ABA catabolism gene ABA8ox1,thus reducing ABA accumulation.In addition,OsbZIP09 also directly bound to the promoter of LEA3 gene to inhibit its expression,thus further alleviating the suppressive effect of ABA on seed germination.These results demonstrated that OsbZIP09 likely functions as a brake of the ABA pathway to attenuate the inhibitory effect of ABA on rice seed germination via dual strategies. | Wang Chuxin Zhu Chengchao Zhou Yu Xiong Min Wang Jindong Bai Huang Lu Chenya Zhang Changquan Liu Qiaoquan Li Qianfeng | 2021 | Rice science2021，28，4： | 1 |
| 13 | Study of the reaction of proteins with BeryllonⅡ-AlⅢby the Rayleigh light scattering technique and its application显示文摘 | Runping Jia Qianfeng Li | 2001 | Analyst2001，126，： | 1 |
| 14 | Brassinosteroid and gibberellin coordinate rice seed germination and embryo growth by regulating glutelin mobilization显示文摘Seed germination is the beginning of a new lifecycle,and involves many complex physiological and biochemical reactions including seed reserve mobilization in the endosperm and nutrient transport and reuse in the embryo.Although glutelin is a dominant storage protein in rice,its contribution to seed germination and its regulatory mechanisms are mostly unknown.Gibberellin (GA) and brassinosteroid (BR),two major growth-promoting phytohormones,also play positive roles in controlling seed germination.However,how GA and BR interact and coordinate seed germination and facilitate glutelin mobilization remains unclear.In the present study,biochemical and physiological analyses of seed germination indicated that both GA and BR promote seed germination and post-germination growth.Exogenous application of GA restored germination defects caused by BR deficiency or insensitivity.Proteomic and q RT-PCR results showed that the expression of several glutelin proteins and their encoding genes was induced by BR and GA in the embryo.Expression assays suggested that the increased accumulation of glutelin protein in the embryo was due to the accelerated degradation of glutelin by a cysteine proteinase (REP-1) in the endosperm.The breakdown of glutelin in the endosperm showed a strict positive correspondence with the length of the shoot.The GluA2 mutation led to reduced degradation rate of glutelin and defects in seed germination,and the promotion effect of GA on seed germination was weakened in the glua2mutant.In vitro culture assay of rice embryos showed that glutelin mobilization functioned downstream of the GA and BR pathways to promote shoot elongation.These findings suggest a mechanism that mediates crosstalk between BR and GA in co-regulating rice seed germination and embryo growth. | Min Xiong Lingyi Chu Qianfeng Li Jiawen Yu Yihao Yang Peng Zhou Yong Zhou Changquan Zhang Xiaolei Fan Dongsheng Zhao Changjie Yan Qiaoquan Liu | 2021 | The Crop Journal2021，9，5： | 1 |
| 15 | Effect of initial gravimetric water content and cyclic wetting-drying on soil-water characteristic curves of disintegrated carbonaceous mudstone显示文摘The soil-water characteristic curve(SWCC)is often used to estimate unsaturated soil properties(e.g.strength,permeability,volume change,solute and thermal diffusivity).The SWCC of soil samples is significantly affected by cyclic wetting-drying.To examine how water content and cyclic wetting-drying affect the SWCC of disintegrated carbonaceous mudstone(DCM),SWCC tests were implemented using a pressure-plate apparatus.In addition,SWCC models for DCM considering the initial gravimetric water content and cyclic wetting-drying were developed.The test results showed that the volumetric water content(θ)of the DCM first decreased rapidly and then became stable as matric suction(s)increased.The initial water content affected the SWCC by altering the pore structure of the DCM.For a given number of wetting-drying cycles,the higher the initial water content,the higher the stabilizedθ.At a given s value,θdecreased as the number of wetting-drying cycles increased,which suggests that cyclic wetting-drying reduces the water-holding capacity of DCM.The Gardner model for DCM was constructed considering initial water content and cyclic wetting-drying,and was effective at describing and predicting the SWCC model for DCM. | Ling Zeng Fan Li Jie Liu Qianfeng Gao Hanbing Bian | 2019 | Transportation Safety and Environment2019，1，3： | 1 |
| 16 | Study of the reaction of proteins with 3-hydroxy-4-(2-hydroxy-4-sulfo-1-naph thalenyl)azo (Cal-Red) by Rayleigh light scattering technique显示文摘 | Lijun DongJiang He Qianfeng Li | 2003 | Analytical Biochemistry2003，，315： | 1 |
| 17 | Ecological sustainability assessment of the carbon footprint in Fujian Province,southeast China显示文摘China’s rapid economic development has initiated the deterioration of its ecological environment,posing a threat to the sustainable development of human society.As a result,an assessment of regional sustainability is critical.This paper researches China’s most forested province,Fujian Province,as the study area.We proposed a grid-based approach to assess the regional carbon footprint in accordance with the Intergovernmental Panel on Climate Change’s(IPCC)carbon emission guidelines.Our method of assessment also introduced carbon emission indicators with our improved and published Net Primary Production(NPP)based on process simulation.The carbon footprint in Fujian Province from 2005-2017 was calculated and examined from a spatiotemporal perspective.Ecological indicators were used in the sustainability assessment.The research draws the following conclusions:1)the carbon footprint in the eastern regions of Fujian Province was higher due to rapid economic development;2)that of the western regions was lower;3)an uptrend in the carbon footprint of Fujian Province was observed.All five ecological indicators based on carbon emissions and economic and social data showed an ecologically unsustainable trend over 13 years in the research area due to unsustainable economic development.Therefore,it is urgent to balance the relationship between economic development and environmental protection.Our research provides scientific references for achieving ecological civilization and sustainability in a similar region. | Jingyu ZENG Rongrong ZHANG Jia TANG Jingchen LIANG Jinghan LI Yue ZENG Yefan LI Qing ZHANG Wei SHUI Qianfeng WANG | 2021 | Frontiers of Earth Science2021，15，1： | 1 |
| 18 | Characterization of expression of the OsPUL gene encoding a pullulanase-type debranching enzyme during seed development and germination in rice显示文摘 | LI Qianfeng ZHANG Guiyun DONG Zhiwei | 2009 | Plant Physiology and Biochemistry2009，47，5： | 1 |
| 19 | Comparison of two methods for the determination of watersoluble organic carbon in atmospheric particles 显示文摘 | YANG Hong LI Qianfeng YU Jianzhen | 2003 | Atmospheric Environment2003，37，6： | 1 |
| 20 | Field-amplified sample stacking in capillary electrophoresis for on-column concentration of alkaloids in Sophora flavescens Air显示文摘 | LIU Shuhui LI Qianfeng CHEN Xingguo | 2002 | Electrophoresis2002，23，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员