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您的检索式：作者名="Qingan Ren"
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| 1 | A relatively high zoonotic trematode prevalence in Orientogalba ollula and the developmental characteristics of isolated trematodes by experimental infection in the animal model显示文摘Background:Food-borne parasitic diseases decrease food safety and threaten public health.The snail species is an intermediate host for numerous human parasitic trematodes.Orientogalba ollula has been reported as intermediate hosts of many zoonotic trematodes.Here,we investigated the prevalence of zoonotic trematodes withinO.ollula in Guangxi,China,and assessed their zoonotic potential.Methods:Snails were collected from 54 sites in 9 cities throughout Guangxi.The snail and trematode larvae species were determined by combining morphological characteristics and molecular markers.The trematodes prevalence and constituent ratio were calculated and compared among different habitat environments.Phylogenetic trees of the trematode species were constructed using the neighbor-joining method with nuclear internal transcribed spacer 2(ITS2)sequences.The developmental cycles of the isolated trematodes were examined by experimental infection in ducks.The developmental characteristics ofEchinostoma revolutum was recorded by dissecting infected ducklings from 1-day post infection(dpi)to 10 dpi.Results:The overall prevalence of trematode larvae was 22.1%(1818/8238)inO.ollula from 11 sample sites.Morphological together with molecular identification,showed thatE.revolutum,Australapatemon sp.,Hypoderaeum conoideum,Pharyngostomum cordatum,andEchinostoma sp.parasitizedO.ollula,with the highest infection rate ofE.revolutum(13.0%).However,noFasciola larvae were detected.The trematodes prevalence and constituent ratio varied in two sub-biotypes(P<0.01).A neighbor-joining tree analysis of ITS2 sequences resulted in distinct monophyletic clades supported by sequences from isolated larvae with high bootstrap values.Ducklings exposed toO.ollula infected withEchinostoma sp.,E.revolutum,andH.conoideum larvae were successfully infected.The animal model forEchinostoma revolutum was successfully established.E.revolutum matured from larvae to adult at 10 dpi in the intestine of the duck,and the developmental characteristics ofE.revolutum were characterized by the maturation of the reproductive and digestive organs at 6-8 dpi.Conclusions:This study revealed a high prevalence of zoonotic trematodes inO.ollula from Guangxi,China.Existing trematodes infection in animals and human clinical cases,coupled with the wide geographical distribution ofO.ollula,necessitate further evaluations of the potential risk of spillover of zoonotic infection from animal to human and vice versa. | Jian Li Yijing Ren Lei Yang Jiani Guo Haiying Chen Jiani Liu Haoqiang Tian Qingan Zhou Weiyi Huang Wei Hu Xinyu Feng | 2022 | Infectious Diseases of Poverty2022，11，4： | 1 |
| 2 | SCGPred:A Score-based Method for Gene Structure Prediction by Combining Multiple Sources of Evidence显示文摘Predicting protein-coding genes still remains a significant challenge. Although a variety of computational programs that use commonly machine learning methods have emerged,the accuracy of predictions remains a low level when implementing in large genomic sequences. Moreover,computational gene finding in newly se-quenced genomes is especially a difficult task due to the absence of a training set of abundant validated genes. Here we present a new gene-finding program,SCGPred,to improve the accuracy of prediction by combining multiple sources of evidence. SCGPred can perform both supervised method in previously well-studied genomes and unsupervised one in novel genomes. By testing with datasets composed of large DNA sequences from human and a novel genome of Ustilago maydi,SCG-Pred gains a significant improvement in comparison to the popular ab initio gene predictors. We also demonstrate that SCGPred can significantly improve predic-tion in novel genomes by combining several foreign gene finders with similarity alignments,which is superior to other unsupervised methods. Therefore,SCG-Pred can serve as an alternative gene-finding tool for newly sequenced eukaryotic genomes. The program is freely available at http://gffzzf1438bbff95247ffh99wow5x66vc66vfv.ffgz.tsg.suse.edu.cn/SCGPred/. | Xiao Li Qingan Ren Yang Weng Haoyang Cai Yunmin Zhu Yizheng Zhang | 2008 | Genomics, Proteomics & Bioinformatics2008，6，3： | 0 |
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