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| 1 | Exosomes derived from human umbilical cord mesenchymal stem cells reduce tendon injuries via the miR-27b-3p/ ARHGAP5/RhoA signaling pathway显示文摘Tendon injuries are common clinical issues resulted from tissue overuse and age-related degeneration.Previous sutdies have suggested that exosomes secreted by mesenchymal stem cells(MSCs)contribute to tissue injury repair.Here,we provide evidence for a critical role of human umbilical cord mesenchymal stem cell(hucMSC)-derived exosomes in reducing tendon injury by activating the RhoA signaling.Treatment of primary injured tenocytes with hucMSC exosomes increases cell proliferation and invasion,which correlates with increased RhoA activity.RhoA mediates the effects of hucMSC exosomes,as treatment of primary injured tenocytes with the RhoA inhibitor,CCG-1423,abolishes the effects of hucMSC exosomes on cell proliferation and invasion.Mechanistically,we observe that hucMSC exosomes induce the expression of a microRNA,miR-27b-3p,which targets and suppresses ARHGAP5,a negative regulator of RhoA.Consistent with this observation,ARHGAP5 overexpression suppresses the effects of hucMSC exosomes on cell proliferation and invasion,while knockdown of ARHGAP5 rescues these effects.Finally,we demonstrate the functional significance of our findings in vivo using an Achilles tendon injury model and show that treatment with exosomes reduces tendon injury in rats,which correlates with increased RhoA activity and reduced ARHGAP5 expression.Taken together,our findings highlight a critical role of hucMSC exosomes in reducing tendon injury via miR-27b-3p-mediated suppression of ARHGAP5,resulting in RhoA activation,and leading to increased cell proliferation and invasion of primary injured tenocytes. | Qinghui Han Shuguang Wang Dawei Chen Di Gan Tao Wang | 2022 | Acta Biochimica et Biophysica Sinica2022，54，2： | 2 |
| 2 | Diethylstilbestrol in Fish Tissue Determined Through Subcritical Fluid Extraction and with GC-MS显示文摘As the key point in sex hormone analysis, sample pre-treatment technology has attracted scientists' attention all over the world, and the development trend of sample preparation forwarded to faster and more efficient technologies. Taking economic and environmental concerns into account, subcritical fluid extraction as a faster and more efficient method has stood out as a sample pre-treatment technology. This new extraction technology can overcome the shortcomings of supercritical fluid and achieve higher extraction efficiency at relatively low pressures and temperatures. In this experiment, a simple, sensitive and efficient method has been developed for the determination of diethylstilbestrol(DES) in fish tissue using subcritical 1,1,1,2-tetrafluoroethane(R134a) extraction in combination with gas chromatography-mass spectrometry(GC-MS). After extraction, freezing-lipid filtration was utilized to remove fatty co-extract. Further purification steps were performed with C_(18) and NH_2 solid phase extraction(SPE). Finally, the analyte was derived by heptafluorobutyric anhydride(HFBA), followed by GC-MS analysis. Response surface methodology(RSM) was employed to optimizing the extraction condition, and the optimized was as follows: extraction pressure, 4.3 MPa; extraction temperature, 26℃; amount of co-solvent volume, 4.7 m L. Under this condition, at a spiked level of 1, 5, 10 μg kg^(-1), the mean recovery of DES was more than 90% with relative standard deviations(RSDs) less than 10%. Finally, the developed method has been successfully used to analyzing the real samples. | QIAO Qinghui SHI Nianrong FENG Xiaomei LU Jie HAN Yuqian XUE Changhu | 2016 | Journal of Ocean University of China2016，15，3： | 1 |
| 3 | Isolation of high-purity casticin from Artemisia annua L . by high-speed counter-current chromatography显示文摘 | Xiao Han Xiaofeng Ma Tianyou Zhang Yabin Zhang Qinghui Liu Yoichiro Ito | 2007 | Journal of Chromatography A2007，，1： | 1 |
| 4 | A novel lytic phage potentially effective for phage therapy against Burkholderia pseudomallei in the tropics显示文摘Background:Burkholderia pseudomallei is a tropical pathogen that causes melioidosis.Its intrinsic drug-resistance is a leading cause of treatment failure,and the few available antibiotics require prolonged use to be effective.This study aimed to assess the clinical potential of B.pseudomallei phages isolated from Hainan,China.Methods:Burkholderia pseudomallei strain(HNBPoo1)was used as the isolation host,and phages were recovered from domestic environmental sources,which were submitted to the host range determination,lytic property assays,and stability tests.The best candidate was examined via the transmission electron microscope for classification.With its genome sequenced and analyzed,its protective efficacy against B.pseudomallei infection in A549 cells and Caenorhabditis elegans was evaluated,in which cell viability and survival rates were compared using the one-way ANOVA method and the log-rank test.Results:A phage able to lyse 24/25 clinical isolates was recovered.It was classified in the Podoviridae family and was found to be amenable to propagation.Under the optimal multiplicity of infection(MOl)of o.1,an eclipse period of around 20 min and a high titer(io12 PFU/ml)produced within 1 h were demonstrated.This phage was found stabile at a wide range of temperatures(24,37,40,50,and 60 C)and pH values(3-12).After being designated as vB_BpP_HN01,it was fully sequenced,and the 71,398 bp linear genome,containing 93 open reading frames and a tRNA-Asn,displayed a low sequence similarity with known viruses.Additionally,protective effects of applications of vB_BpP_HNo1(MOl=0.1 and MOl=1)alone or in combination with antibiotics were found to improve viability of infected cells(70.6±6.8%,85.8±5.7%,91.9±1.8%,and 96.8±1.8%,respectively).A significantly reduced mortality(10%)and a decreased pathogen load were demonstrated in infected C.elegans following the addition of this phage.Conclusions:As the frst B.pseudomallei phage was isolated in Hainan,China,phage vB_BpP_HNO1 was characterized by promising lytic property,stability,and effciency of bacterial elimination during the in vitro/vivo experiments.Therefore,we can conclude that it is a potential alternative agent for combating melioidosis. | Yanshuang Wang Xuemiao Li David A.B.Dance Han Xia Chen Chen Nini Luo Anyang Li Yanmei Li Qiao Zhu Qinghui Sun Xingyong Wu Yingfei Zeng Lin Chen Shen Tian Qianfeng Xia | 2022 | Infectious Diseases of Poverty2022，11，4： | 0 |
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