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| 1 | Morphological evolution of γ'precipitates in a nickel-based superalloy during various solution treatments显示文摘The morphological evolution of the γ' phase in nickel-based superalloy жc6y during various solution heat treatments was investigated. The significant changes of the γ' precipitates were observed in the solution-treated samples. The coarsening and dissolution of γ' phase simulta-neously occurred at intermediate temperatures. In some areas, the primary precipitates became blunt and the adjacent ones were intercon-nected with each other via a diffuse neck, indicating a coarsening process of the primary γ' population. The coarsening was dominated by the precipitate agglomeration mechanism (PAM) rather than by the well-known Ostwald ripening mechanism. In other areas, the partial dissolu-tion of the γ' precipitates began to occur, spreading gradually from dendrite cores to interdendritic regions. In addition, a flower-like γ' struc-ture was developed during the subsolvus solution treatments. The observable long filaments composed of erraticly shaped precipitates were caused by the heterogeneous nucleation of the cooling precipitates during water quenching. | Zhou, Yu Zhang, Zheng Zhao, Zihua Zhong, Qunpeng | 2012 | Rare Metals2012，31，3： | 10 |
| 2 | Improved Reliability Analysis Method Based on the Failure Assessment Diagram显示文摘With the uncertainties related to operating conditions,in-service non-destructive testing(NDT) measurements and material properties considered in the structural integrity assessment,probabilistic analysis based on the failure assessment diagram(FAD) approach has recently become an important concern.However,the point density revealing the probabilistic distribution characteristics of the assessment points is usually ignored.To obtain more detailed and direct knowledge from the reliability analysis,an improved probabilistic fracture mechanics(PFM) assessment method is proposed.By integrating 2D kernel density estimation(KDE) technology into the traditional probabilistic assessment,the probabilistic density of the randomly distributed assessment points is visualized in the assessment diagram.Moreover,a modified interval sensitivity analysis is implemented and compared with probabilistic sensitivity analysis.The improved reliability analysis method is applied to the assessment of a high pressure pipe containing an axial internal semi-elliptical surface crack.The results indicate that these two methods can give consistent sensitivities of input parameters,but the interval sensitivity analysis is computationally more efficient.Meanwhile,the point density distribution and its contour are plotted in the FAD,thereby better revealing the characteristics of PFM assessment.This study provides a powerful tool for the reliability analysis of critical structures. | ZHOU Yu ZHANG Zheng ZHONG Qunpeng | 2012 | Chinese Journal of Mechanical Engineering2012，25，4： | 9 |
| 3 | Detection and Analysis of Routine Blood Indexes in Guike Pig显示文摘[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells( red blood cells,white blood cells and platelets),thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs( 22 males and 22 females) once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count( RBC) and hemoglobin count( HGB) both reached the lowest levels( 6. 85 × 1012/L and 97. 1 g/L)at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age( P < 0. 05,similarly hereinafter); mean corpuscular hemoglobin( MCH) and mean corpuscular hemoglobin concentration( MCHC) demonstrated basically the same variation pattern,which both reached the lowest levels( 15. 11 pg and 275. 70 g/L) at 70 days of age and reached the highest levels( 16. 40 pg and 314. 30 g/L) at 91 days of age; hematocrit( HCT),mean corpuscular volume( MCV) and red blood cell distribution width( RDW) exhibited relatively stable changes with no significant differences among different day ages( P > 0. 05,similarly hereinafter). Among white blood cell indexes of Guike pig at different day ages,white blood cell count( WBC) reached the lowest level( 13. 71 × 109/L) at 63 days of age and reached the highest level( 17. 52 × 109/L) at 84 days of age,with significant differences; neutrophil count( NEUT)reached the lowest level( 3. 61 × 109/L) at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count( LYMPH) exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexes of Guike pig at different day ages,platelet count( PLT),thrombocytocrit( PCT) and platelet volume distribution width( PDW) exhibited relatively stable changes among different day ages,which were not significantly affected by the increase in day age. Compared to 35-day-old Landrace pig and 5-month-old Enshi black pig,most routine blood indexes of Guike pig were similar to or ranged between those of Landrace pig and Enshi black pig except HCT,mean platelet volume( MPV),PCT and PDW. [Conclusions]Guike pig has a certain uniqueness in routine blood indexes,which has developed a stable immune system at 49-91 days of age. | Qianlian SU Bin LI Jiaxing LIANG Yingning ZHOU Zhongwei CHEN Yibin QIN Bingxia LU Ying HE Qunpeng DUAN Dongfu JIANG Jingzhuan LU Wu ZHAO | 2018 | Agricultural Biotechnology2018，7，3： | 6 |
| 4 | Detection of Porcine Epidemic Diarrhea Virus in Guangxi Province from 2011 to 2014 and Sequence Analysis of Its M Gene显示文摘Detection of pigs epidemic diarrhea virus( PEDV) was conducted on 331 piglets diarrhea fecal samples collected in Nanning,Yulin and other 12 areas of Guangxi Province from January of 2011 to April of 2014 by the method of reverse transcription-polymerase chain reaction( RT-PCR). The results showed that the positive samples of PEDV were 210 and the positive rate was 63. 44%. The clone and sequencing of M gene was carried out on 25 positive samples. PEDV reference strains were selected from Gene Bank to conduct the sequence homology alignment analysis and the phylogenetic tree of M gene. The M gene homology and amino acid sequence identity between 25 isolated strains and 51 reference strains were 96. 0%- 99. 6% and 94. 3%- 99. 6%,respectively. The genetic variation analysis of M gene showed that the genetic relationship of PEDV prevalent strains in Guangxi Province from 2013 to 2014 was close to that of the prevalent strains in Beijing,Anhui,Wuhan,Hebei and Guangdong from 2010 to 2013,and which were far from that of the Chinese early isolates CH / S( Gen Bank number: JN547228),vaccine strain CV777( Gen Bank number: AF353511) and Attenuated DR13( Gen Bank number: JQ023162). Indicating that the PEDV strains prevalent in Guangxi in recent years showed significant variation with the early isolates. | Lu Bingxia Qin Yibin He Ying Li Yingying Liang Jiaxing Li Keyu Li Bin Su Qianlian Zhou Yingning Jiang Dongfu Lu Jingzhuan Bi Bingfen Liang Baozhong Duan Qunpeng Zhao Wu | 2016 | Animal Husbandry and Feed Science2016，8，1： | 3 |
| 5 | Characterization of Passive Films Formed on As-received and Sensitized AISI 304 Stainless Steel显示文摘The current research of corrosion resistance of stainless steels mainly focuses on characterization of the passive films by point defect mode and mixed-conduction model. The corrosion resistance of the passive films formed on asreceived and sensitized AISI304 stainless steel in borate bu er solution were evaluated in this paper. The degree of sensitization and corrosion resistance of AISI304 stainless steels was evaluated by double loop electrochemical potentiodynamic reactivation and electrochemical impedance spectroscopy. The passive films formed on the stainless steels were studied by XPS technique. It was found that as-received specimen had higher pitting corrosion potential and corrosion resistance than sensitized one. The Mott-Schottky results showed that sensitized stainless steel had more defects in the passive film than as-received one. The compositions of the passive films were mainly Cr and Fe oxides according to XPS results. | Yubo Zhang Hongyun Luo Qunpeng Zhong Honghui Yu Jinlong Lv | 2019 | Chinese Journal of Mechanical Engineering2019，32，2： | 2 |
| 6 | Two new triazolophanes: synthesis, structures, self-assembling, and anion complexation properties显示文摘 | Qunpeng Duan Wei Xia Chen Lin Yi Pan Leyong Wang | 2015 | Tetrahedron Letters2015，，26： | 1 |
| 7 | The effect of burnishing parameters on burnishing force and surface microhardness显示文摘 | Hongyun Luo Jianying Liu Lijiang Wang Qunpeng Zhong | 2006 | The International Journal of Advanced Manufacturing Technology (-)2006，，7： | 1 |
| 8 | Isolation of Swine Corynebacteria and Analysis of 16S rDNA Gene显示文摘A strain of gram-positive bacillus was isolated from suppurative lung organs of nursery pigs in a pig farm,which was further characterized by morphological observation,cultivation test,biochemical test,drug sensitivity test,pathogenicity test and 16 S rDNA gene cloning and sequence analysis. The results showed that the isolate grew well in rabbit blood agar plate and horse serum tryptone soybean agar( TSA) plate under aerobic condition,which could lead to mortality of mice and were susceptible to cephalosporin antibiotics and fluoroquinolone antibiotics. Phylogenetic analysis showed that the isolate had close genetic evolutionary relationships with Corynebacterium bacteria,and the sequence of 16 S rRNA gene shared the homology of 91. 7%-98. 3% with the representative strain of corynebacteria,indicating the isolated strain was corynebacterium. | Qin Yibin He Pingping Lu Bingxia He Ying Li Bin Liang Jiaxing Duan Qunpeng Chen Zhongwei Bi Bingfen Zhou Yingning Su Qianlian Jiang Dongfu Li Keyu Lu Jingzhuan Zhao Wu | 2016 | Animal Husbandry and Feed Science2016，8，6： | 0 |
| 9 | Phylogenetic Analysis of HN Gene of Eight Pigeon NDV Isolates in Guangxi显示文摘[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease(ND).[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR,sequenced and analyzed.The molecular evolution characteristics of HN gene of pigeon NDV isolates in Guangxi was discussed.[Result] The nucleotide sequence length of HN gene of the eight NDV isolates was 1 716 bp,encoding 571 amino acids.They belonged to virulent group C,and the gene length characteristic of HN gene accorded with virulent strain.Analysis of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb,ranging from 90.4% to 99.5%.Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Gangxi and the NDV isolates from Guangxi,Guangdong,Jilin,Liaoning,Yunnan and Heilongjiang during 2011 and 2013 was close.They were located in the same cladogram branch.[Conclusion] We assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV. | Lu Bingxia Liang Jiaxing Duan Qunpeng Chen Zhongwei Jiang Dongfu Lu Jingzhuan Zhou Yingning Bi Bingfen He Ying Qin Yibin Li Bin Su Qianlian Zhao Wu | 2018 | Animal Husbandry and Feed Science2018，10，2： | 0 |
| 10 | Construction of Three Nucleic Acid Vaccines of Swine Hepatitis E Virus ORF2 Ger\e Continuous Fragment显示文摘In order to develop swine hepatitis E( HE) genetically engineering vaccines,specific primers of genes LB1,LB2,LB3 of swine hepatitis E virus were designed and used for amplification,DNA amplicons generated by PCR assays were directly cloned into T-A plasmid and expressed using pE ASY-M1 expression vector. Three recombinant eukaryotic expression plasmids of pE ASY-LB1,pE ASY-LB2 and pE ASY-LB3 were constructed. The eukaryotic expression plasmids of pE ASY-LB1,pE ASY-LB2,and pE ASY-LB3 were transfected into 293 T cells,and three target genes were detected by real-time fluorescent quantitative RT-PCR.The results confirmed that three eukaryotic expression plasmids were transfected into 293 Tcells and target protein was expressed. Analysis by SDS-PAGE electrophoresis and Western-blot indicated that three target proteins were expressed in 293 T cells transfected with eukaryotic expression plasmids of pE ASY-LB1,pE ASY-LB2 and pE ASY-LB3. Antigenicity studies indicated good HEV responses. Therefore,three recombinant DNAs of HEV ORF2 nucleic acid vaccine candidates were obtained,which might lay the foundation for further studies in the future. | Li Bin Su Qianlian Zhao Wu Qin Yibin Liang Jiaxing Xiao Aihuan Lu Bingxia Li Yingying He Ying Duan Qunpeng Jiang Jiajia Liang Baozhong | 2015 | Animal Husbandry and Feed Science2015，7，5： | 0 |
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