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| 1 | m TOR signaling in liver regeneration: Rapamycin combined with growth factor treatment显示文摘AIM: To investigate the effects of mammalian target of rapamycin(mT OR) inhibition on liver regeneration and autophagy in a surgical resection model.METHODS: C57BL/6 mice were subjected to a 70% partial hepatectomy(PH) and treated intraperitoneally every 24 h with a combination of the m TOR inhibitor rapamycin(2.5 mg/kg per day) and the steroid dexamethasone(2.0 mg/kg per day) in phosphate bufferedsaline(PBS) or with PBS alone as vehicle control. In the immunosuppressant group, part of the group was treated subcutaneously 4 h prior to and 24 h after PH with a combination of human recombinant interleukin 6(IL-6; 500 μg/kg per day) and hepatocyte growth factor(HGF; 100 μg/kg per day) in PBS. Animals were sacrificed 2, 3 or 5 d after PH and liver tissue and blood were collected for further analysis. Immunohistochemical staining for 5-Bromo-2'-deoxyuridine(Brd U) was used to quantify hepatocyte proliferation. Western blotting was used to detect hepatic microtubule-associated protein 1 light chain 3(LC3)-Ⅱ protein expression as a marker for autophagy. Hepatic gene expression levels of proliferation-, inflammation- and angiogenesisrelated genes were examined by real-time reverse transcription-polymerase chain reaction and serum bilirubin and transaminase levels were analyzed at the clinical chemical core facility of the Erasmus MC-University Medical Center.RESULTS: m TOR inhibition significantly suppressed regeneration, shown by decreased hepatocyte proliferation(2% vs 12% Brd U positive hepatocyte nuclei at day 2, P < 0.01; 0.8% vs 1.4% at day 5, P = 0.02) and liver weight reconstitution(63% vs 76% of initial total liver weight at day 3, P = 0.04), and furthermore increased serum transaminase levels(aspartate aminotransferase 641 U/L vs 185 U/L at day 2, P = 0.02). Expression of the autophagy marker LC3-Ⅱ, which was reduced during normal liver regeneration, increased after mT OR inhibition(46% increase at day 2, P = 0.04). Hepatic gene expression showed an increased inflammation-related response [tumor necrosis factor(TNF)-α 3.2-fold upregulation at day 2, P = 0.03; IL-1Ra 6.0-fold upregulation at day 2 and 42.3-fold upregulation at day 5, P < 0.01] and a reduced expression of cell cycle progression and angiogenesis-related factors(HGF 40% reduction at day 2; vascular endothelial growth factor receptor 2 50% reduction at days 2 and 5; angiopoietin 1 60% reduction at day 2, all P ≤ 0.01). Treatmentwith the regeneration stimulating cytokine IL-6 and growth factor HGF could overcome the inhibitory effect on liver weight(75% of initial total liver weight at day 3, P = 0.02 vs immunosuppression alone and P = 0.90 vs controls) and partially reversed gene expression changes caused by rapamycin(TNF-α and IL-1Ra levels at day 2 were restored to control levels). However, no significant changes in hepatocyte proliferation, serum injury markers or autophagy were found.CONCLUSION: mT OR inhibition severely impairs liver regeneration and increases autophagy after PH. These effects are partly reversed by stimulation of the IL-6 and HGF pathways. | Suomi MG Fouraschen Petra E de Ruiter Jaap Kwekkeboom Ron WF de Bruin Geert Kazemier Herold J Metselaar Hugo W Tilanus Luc JW van der Laan Jeroen de Jonge | 2013 | World Journal of Transplantation2013，3，3： | 6 |
| 2 | Dynamic chromatin states in human ES cells reveal potential regulatory sequences and genes involved in pluripotency显示文摘Pluripotency,一个细胞的能力区分并且产生所有胚胎的系,定义象胚胎的茎(ES ) 那样的哺乳动物的细胞类型的一个小数字细胞。当它通常被保持了时,那 pluripotency 是 transcriptional 的产品激活并且维持关键干细胞基因的表达式的规章的网络,积累的证据在建立并且保卫 ES 房间的 pluripotency 为 epigenetic 进程正在指向一个关键角色,象维持区分的房间类型的身份一样。以便更好在 pluripotency 理解 epigenetic 机制的角色,我们检验了在经历区别进一个 mesendodermal 系的人的 ES 房间(hESCs ) 染色体宽的染色质修正的动力学。我们发现在倡导者的染色质修正在区别期间仍然保持大部分不变,除了在在在 H3K27 的 acetylation 和 methylation 之间的一个动态开关标记在基因表示的激活和 silencing 之间的转变的倡导者的一个小数字,建议在在大多数差别上的房间命运承诺的一个层次表示了基因。我们也在 50 000 潜在的 enhancers 上印射,并且在染色质修正,特别 H3K4me1 和 H3K27ac 观察了大得多的动力学,它与他们的潜在的目标基因的表示相关。这些 enhancers 的进一步的分析揭示了 pluripotency 和可以在 hESCs 授与发展胜任的一个平衡状态的一口染色质签名陈述语气的潜在地关键的 transcriptional 管理者。我们的结果提供在定义 enhancers 和 pluripotency 支持染色质修正的角色的新证据。 | R David Hawkins Gary C Hon Chuhu Yang Jessica E Antosiewicz-Bourget Leonard K Lee Que-Minh Ngo Sarit Klugman Keith A Ching Lee E Edsall Zhen Ye Samantha Kuan Pengzhi Yu Hui Liu Xinmin Zhang Roland D Green Victor V Lobanenkov Ron Stewart James A Thomson Bing Ren | 2011 | Cell Research2011，21，10： | 6 |
| 3 | Characterization and target identification of a DNA aptamer that labels pluripotent stem cells显示文摘 | Zhonggang Hou Susanne Meyer Nicholas E Propson jeff Nie Peng Jiang Ron Stewart James A Thomson | 2015 | Cell Research2015，25，3： | 5 |
| 4 | Effects of pretreatment with clopidogrel and aspirin followed by long-term therapy in patients undergoing percutaneous coronary intervention: the PCI-CURE study显示文摘 | Shamir R Mehta Salim Yusuf Ron JG Peters Michel E Bertrand Basil S Lewis Madhu K Natarajan Klas Malmberg Hans-Jürgen Rupprecht Feng Zhao Susan Chrolavicius Ingrid Copland Keith AA Fox | 2001 | The Lancet . 2001 (9281)2001，，9281： | 4 |
| 5 | Preservation of sperm of cancer patients: extent of use and pregnancy outcome in a tertiary infertility center显示文摘精子 cryopreservation 是最好的形式为与癌症诊断的男性保证未来生育力。cryopreserved 精子实际上为受精,可得到的富饶治疗选择和这些治疗的成功率被使用到的程度没深入地被调查。谁的 682 个病人的医药记录 cryopreserved 精子房间由于癌症,治疗被分析。七十个这些病人在 20 年的经期(在在 cryopreservation 以后的开始的 4 年以内的大多数) 上为生育力治疗撤退了他们的冻结的精子。不同恶意的精子质量和为在与治疗的类型和恶意的类型的关系的怀孕成就的帮助繁殖治疗(艺术) 的结果被评估。结果证明在我们的单位为富饶治疗从癌症病人使用融化 cryo 的精子的率是 10.3 %。精子优秀索引在恶意的不同类型之间不同,与在阴囊的癌症测量的最差的质量。概念在 184 个艺术周期中的 46 个被完成(25 %) ,并且导致了 36 交货。注射(ICSI ) 方法论产出的 intracytoplasmic 精子的使用显著地更高的怀孕率(37.4 %) 比子宫内的授精(IUI;11.5 %) 并且类似于用这些形式的其它组不肥沃的夫妇。在 vitro,授精(IVF ) 没能生产怀孕。在结论,在癌症病人的 cryopresseved 精子的使用的率是相对低的(10.3 %) 。由 ICSI 的怀孕的成就介绍最好的选择但是当有足够的存储精子样品和足够的质量时, IUI 能被采用。Cryopreservation 是但为癌症病人保存未来生育力潜力和希望的最好的选择。 | Amnon Botchan Shiri Karpol Ofer Lehavi Gedalia Paz Sandra E Kleiman LeahYogev Haim Yavetz Ron Hauser | 2013 | Asian Journal of Andrology2013，15，3： | 4 |
| 6 | Cerebral magnetic resonance imaging in quiescent Crohn's disease patients with fatigue显示文摘AIM To evaluate brain involvement in quiescent Crohn's disease (CD) patients with fatigue using quantitative magnetic resonance imaging(MRI).METHODS Multiple MRI techniques were used to assess cerebral changes in 20 quiescent CD patients with fatigue(defined with at least 6 points out of an 11-point numeric rating scale compared with 17 healthy age and gender matched controls without fatigue. Furthermore, mental status was assessed by cognitive functioning, based on the neuropsychological inventory including the different domains global cognitive functioning, memory and executive functioning and in addition mood and quality of life scores. Cognitive functioning and mood status were correlated with MRI findings in the both study groups.RESULTS Reduced glutamate + glutamine (Glx = Glu + Gln) concentrations (P = 0.02) and ratios to total creatine(P = 0.02) were found in CD patients compared with controls. Significant increased Cerebral Blood Flow(P = 0.05) was found in CD patients (53.08 ± 6.14 mL/100 g/min) compared with controls (47.60 ± 8.62 mL/100 g/min). CD patients encountered significantly more depressive symptoms (P < 0.001). Cognitive functioning scores related to memory (P = 0.007) and executive functioning (P = 0.02) were lower in CD patients and both scores showed correlation with depression and anxiety. No correlation was found subcortical volumes between CD patients and controls in the T1-weighted analysis. In addition, no correlation was found between mental status and MRI findings.CONCLUSION This work shows evidence for perfusion, neurochemical and mental differences in the brain of CD patients with fatigue compared with healthy controls. | Sanne van Erp Ece Ercan Perla Breedveld Lianne Brakenhoff Eidrees Ghariq Sophie Schmid Matthias van Osch Mark van Buchem Bart Emmer Jeroen van der Grond Ron Wolterbeek Daniel Hommes Herma Fidder Nic van der Wee Tom Huizinga Désirée van der Heijde Huub Middelkoop Itamar Ronen Andrea van der Meulen-de Jong | 2017 | World Journal of Gastroenterology2017，23，6： | 2 |
| 7 | Temperature-responsive gels and thermogelling polymer matrices for protein and peptide delivery 显示文摘 | BROMBERG L E RON E S | 1998 | Advanced Drug Delivery Reviews1998，31，3： | 1 |
| 8 | Multiple primary breast andthyroid cancer 显示文摘 | Ron E Curtis R Hoffman DA | 1984 | Br J Cancer1984，49，1： | 1 |
| 9 | Proliferative activity of human thy- roid cells in various age groups and its correlation with the risk of thyroid cancer after radiation exposure 显示文摘 | Saad AG Kumar S Ron E | 2006 | J Clin Endocrinol Metab2006，91，7： | 1 |
| 10 | Antibiotics against Pseudomonas aeruginosa for COPD exacerbation in ICU:a 10-year retrospective study显示文摘 | Planquette B Péron J Dubuisson E | 2015 | Int J Chron Obstruct Pulmon Dis2015，10，： | 1 |
| 11 | Molecular tools in rhizosphere microbiology-from single-cell to whole-community analysis显示文摘 | Sorensen J Nicolaisen M H Ron E | 2009 | Plant Soil2009，321，12： | 1 |
| 12 | Natural Role of Biosurfactants显示文摘 | RON E Z ROSENBERG E | 2001 | Environ Microbiol2001，3，4： | 1 |
| 13 | Rising thyroid cancer incidence in the united states by demographic and tumor charactristics, 1980 : 2005 显示文摘 | Enewold L Zhu K Ron E | 2009 | Cancer Epidemiol Biomarkers Prey2009，18，3： | 1 |
| 14 | Nonradiation risk factors for thyroid cancer in the US Radiologic Teehnol ogistsStudy显示文摘 | Meinhold C L Ron E Schonfeld S J | 2010 | Am J Epidemiol2010，171，2： | 1 |
| 15 | Molecular tools in rhizosphere microbiology - from single - cell to whole - conununity analysis显示文摘 | Sorensen J Nicolaisen M H Ron E | 2009 | Plant and Soil2009，321，12： | 1 |
| 16 | Experimental studies on the role of backfill and pipeline characteristics in the application of acoustic leak location to under around pipelines显示文摘 | Clark M Amanda Rewerts Lance E Robbers Ron R | 1997 | Review of Progress in Quantitative Non-destructive Evaluation1997，16，4： | 1 |
| 17 | Solid cancer incidence in atomic bomb survivors:1958-1998显示文摘 | Preston DL Ron E Tokuoka S | 2007 | Radiat Res2007，168，1： | 1 |
| 18 | Continuous Path Verification in Multi-axis NC-machining显示文摘 | Ron W Gershon E | | 0，，04： | 1 |
| 19 | Thyroid cancer and multiple primary tumors in the SEER cancer registries显示文摘 | Ronckers CM Mccarron P Ron E | 2005 | Int J Cancer2005，117，2： | 1 |
| 20 | Permeability and thermal conductivity of porous metallicmatrix hydride compacts 显示文摘 | Bershadsky E Josephy Y Ron M | 1989 | Journal of the Less-Common Metals1989，153，1： | 1 |
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