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| 1 | Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease显示文摘AIM:To investigate intestinal alkaline phosphatase(iAP) in the intestinal mucosa of children with inflammatory bowel disease(IBD).METHODS:Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls.In IBD patients,specimens were obtainedboth from inflamed and non-inflamed areas.The iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis,respectively.Tissue localization of iAP and Toll-like receptor(TLR) 4 was investigated by immunofluorescent staining.RESULTS:The iAP protein level in the inflamed mucosa of children with Crohn's disease(CD) and ulcerative colitis(UC) was significantly decreased when compared with controls(both P < 0.05).Similarly,we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD(P < 0.05).In addition,the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD(P < 0.05).iAP protein levels in the non-inflamed mucosa of patients with CD were similar to controls.iAP mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with CD.Expression of iAP mRNA in patients with noninflamed mucosa and in controls were similar.Co-localization of iAP with TLR4 showed intense staining with a dotted-like pattern.iAP was present in the inflamed and non-inflamed mucosa of patients with CD,UC,and in control biopsy specimens,irrespective of whether it was present in the terminal ileum or in the colon.However,the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups studied.CONCLUSION:Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in IBD.Based on our results,administration of exogenous iAP enzyme to patients with the active form of IBD may be a therapeutic option. | Kriszta Molnár dám Vannay Beáta Szebeni Nóra Fanni Bánki Erna Sziksz ron Cseh Hajnalka Gyrffy Péter László Lakatos Mária Papp András Arató Gábor Veres | 2012 | World Journal of Gastroenterology2012，18，25： | 5 |
| 2 | Soil zoology:an indispensable component of integrated ecosystem studies显示文摘 | RON G G L IJBERT B | 2002 | European Journal of Soil Biology2002，38，： | 1 |
| 3 | Identification of genes potentially involved in rupture of human atherosclerotic plaques显示文摘 | Birgit C G F Kitty B J M C Ron L J N | 2001 | Circ Res2001，89，6： | 1 |
| 4 | Volatile constituents of Asian pear(Pyrus serotina)显示文摘 | Gary R T Ron G B Robert A F | 1992 | Food Chemistry1992，4，： | 1 |
| 5 | Manipulations of AchE gene expression suggest noncatalytive of acetycholiesterase in the functioning of mammailian photoreceptors but not in retinal degeneration显示文摘 | Ron S B Mitra G Anat L | 1999 | Molecular Brain Res1999，71，1： | 1 |
| 6 | Macadamia nutconsumption lowers plasma total and LDL cholesterol levels inhypercholesterolemic men显示文摘 | Manohar L G Robert J B Ron B H W | 2003 | The Journal of Nutrition2003，133，4： | 1 |
| 7 | Macadamia nut consumption lowers plasma total and LDL cholesterol levels in hypereholesterolemic men显示文摘 | Manohar L G Robert J B Ron B H W | 2003 | The journal of Nutrition2003，133，4： | 1 |
| 8 | Macadamia nutconsumption modulates favourably risk factors for coronary arterydisease in hypercholesterolemic subjects显示文摘 | Mannhar L G Robert J B Ron B H W | 2007 | Lipids2007，42，6： | 1 |
| 9 | Macadamia nut consumption modulates favourably risk factors for coronary artery disease in hypercholesterolemic subjects显示文摘 | Mannhar L G Robert J B Ron B H W | 2007 | Lipids2007，42，6： | 1 |
| 10 | Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays)显示文摘 | ROnning S B Vatilingom M Berdal K G | 2003 | European Food Research Technology2003，216，： | 1 |
| 11 | A new and versatile method for the successful conversion of AFLP markers into simple single locus markers 显示文摘 | Brugmans B van der Hulst Ron G M Visser Richard G F | 2003 | Nucleic Acids Research2003，31，10： | 1 |
| 12 | Effect of gypsum on proliferation and differentiation of MC3T3-E1 mouse osteoblastic cells显示文摘 | áron Lazáry Bernadett Balla János P. Kósa Krisztián Bácsi Zsolt Nagy István Takács Péter P. Varga Gábor Speer Péter Lakatos | 2006 | Biomaterials2006，，3： | 1 |
| 13 | Additional aroma components of honeydew melon显示文摘 | RON G B RICHARD M S LOUISA C | 1982 | J Agric Food Chem1982，30，6： | 1 |
| 14 | Seasonal variation of antifouling activities of marine algae from the Brittany coast (France) 显示文摘 | Hellio C Marechal JP Véron B Bremer G Clare AS Le Gal Y | 2004 | Mar Biotechnol2004，6，1： | 1 |
| 15 | Event specific real-time quantitative PCR for genetically modified Bt11 maize ( Zea mays ) 显示文摘 | Ronning S B Vaitilingom M Berdal K G | 2003 | Euro Food Res Technol2003，216，4： | 1 |
| 16 | Macadamia nut consumption lowers plasma total and LDL cholesterol levels in hypercholesterolemic men显示文摘 | Manohar L G Robert J B Ron B H W | 2003 | The journal of Nutrition2003，133，4： | 1 |
| 17 | Macadamia nut consumption modulates favourably risk factors for coronary artery disease in hypercholesterolemic subjects显示文摘 | Mannhar L G Robert J B Ron B H W | 2007 | Lipids2007，42，6： | 1 |
| 18 | Event specific real-time quantitative PCR for genetically modified Btll maize ( Zea mays) 显示文摘 | Ronning S B Vaitilingom M Berdal K G | 2003 | European Food Research and Technology2003，216，： | 1 |
| 19 | Novel reference gene,PKABA1,used in a duplex real-time polymerase chain reaction for detection and quantitation of wheat-and barley-derived DNA显示文摘 | Ronning S B Berdal K G Andersen C B | 2006 | Journal of Agricultural and Food Chemistry2006，54，3： | 1 |
| 20 | Combinatorial engineering to enhance amylosucrase performance:construction,selection,and screening of variant libraries for increased activity显示文摘 | Van Der Veen B A Potocki-Véronèse G Albenne C | 2004 | FEBS Letters2004，560，1： | 1 |
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