|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Rat model of cholelithiasis with human gallstones implanted in cholestasis-induced virtual gallbladder显示文摘AIM:To facilitate translational research on cholelithiasis,we have developed a rat model of human gallstones by exploiting the unique biliopancreatic features of this species.METHODS:Under anesthesia,16 adult rats of equal genders underwent two times of abdominal surgery.First,their common bile duct(CBD)was ligated to cause cholestasis by total biliary obstruction(TBO).On day 0,1,3,7,14,21 and 28 after TBO,magnetic resonance imaging(MRI)was conducted to monitor the dilatation of the CBD,and blood was sampled to analyze total serum bilirubin(TSB).Secondly,on day 30,the abdomen was re-opened and gallstone(s)collected from human patients were implanted in the dilated CBD asa virtual gallbladder(VGB),which was closed by suture ligation.This rat cholelithiasis model was examined by MRI,clinical observation,microcholangiography and histology.RESULTS:All rats survived two laparotomies.After ligation,the CBD was dilated to a stable size of 4 to30 mm in diameter on day 21-28,which became a VGB.The rats initially showed signs of jaundice that diminished over time,which paralleled with the evolving TSB levels from 0.6±0.3 mg/d L before ligation,through a peak of 10.9±1.9 mg/dL on day 14,until a nearly normalized value after day 28.The dilated CBD with thickened wall allowed an incision for implantation of human gallstones of 1-10 mm in diameter.The rat cholelithiasis was proven by in vivo MRI and postmortem microcholangiography and histomorphology.CONCLUSION:A rat model cholelithiasis with human gallstones has been established,which proves feasible,safe,reliable,nontoxic and cost-effective.Given the gallstones of human origin,applications of this model may be of help in translational research such as optical detection and lysis of gallstones by systemic drug administration. | Marlein Miranda Cona Yewei Liu Ting Yin Yuanbo Feng Feng Chen Stefaan Mulier Yue Li Jian Zhang Raymond Oyen Yicheng Ni | 2016 | World Journal of Methodology2016，6，2： | 7 |
| 2 | A long-form α-neurotoxin from cobra venom produces potent opioid-independent analgesia显示文摘Aim:In light of the antinociceptive activity of the short-chain neurotoxin,cobrotoxin,and other acetylcholine antagonists,the antinociceptive activity andmechanisms of cobratoxin(CTX),a long-chain postsynaptic α-neurotoxin,wasinvestigated in rodent pain models.Methods:CTX was administered intraperito-neally(30,45,68 μg/kg),intra-cerebral ventricularly(4.5 μg/kg)or microinjectedinto periaqueductal gray(PAG;4.5 μg/kg).The antinociceptive action was testedusing the hot-plate and acetic acid writhing tests m mice and rats.The involve-ment of the cholinergic system and opioid system in CTX-induced analgesia wasexamined by pretreatment of animals with atropine(0.5 mg/kg,im;or 10 mg/kg,ip)or naloxone(1 and 5 mg/kg,ip).The effect of CTX on motor activity was testedusing the Animex test.Results:CTX exhibited a dose-dependent analgesic ac-tion in mice as determined by both the hot-plate and acetic acid writhing tests.The peak effect of analgesia was seen 3 h after administration.In the mouse aceticacid writhing test,the intra-cerebral ventricular administration of CTX at 4.5 μg/kg(1/12th of a systemic dose)produced marked analgesic effects.Microinjection ofCTX(4.5 μgkg)into the PAG region did not elicit an analgesic action in rats in thehot-plate test.Atropine at 0.5 mg/kg(im)and naloxone at 1 and 5 mg/kg(ip)bothfailed to block the analgesic effects of CTX,but atropine at 10 mg/kg(ip)didantagonize the analgesia mediated by CTX in the mouse acetic acid writhing test.Acetylsalicylic acid(300 mg/kg)did not enhance the analgesic effects of CTX.Atthe highest effective dose of 68 μg/kg the neurotoxin did not change the sponta-neous mobility of mice.Conclusion:CTX has analgesic effects,which are medi-ated in the central nervous system though not through the PAG.The centralcholinergic system but not opioid system appears to be involved in theantinociceptive action of CTX. | Zhi-xin CHEN Hui-ling ZHANG Zhen-lun GU Bo-wen CHEN Rong HAN Paul F REID Laurence N RAYMOND Zheng-hong QIN | 2006 | Acta Pharmacologica Sinica2006，27，4： | 7 |
| 3 | An efficient method for privacy preserving location queries显示文摘最近,保存地点询问的隐私的问题吸引了许多研究。然而,有很少工作,集中于在地点隐私保藏和地点质问信息收集之间的折衷。处理这种折衷,我们建议坚持的隐私地点质问(PLQ ) ,保存地点质问处理的有效隐私框架。没有揭示用户地点信息,这个框架能启用基于地点的质问。框架能也便于基于地点的服务供应商关于基于的地点收集一些信息查询,它在实践是有用的。也就是, PLQ 由三个关键部件组成在顾客方面,在服务器方面的隐私询问处理器,和连接顾客和服务器的另外的信任第三方的地点 anonymizer。地点 anonymizer 基于一个地图层次模糊用户地点进一个 cloaked 区域。地图层次包含根据真实地形被划分的精确区域。隐私质问处理器处理请求近邻居(NN ) 地点基于质问。多角形(CHP ) 的新凸的壳算法用一个多角形 cloaked 区域为近邻居的询问被建议。我们高效地,进程地点基于的算法罐头查询的试验性的结果表演。 | Yubao LIU Xiuwei CHEN Zhan LI Zhijie LI Raymond Chi-Wing WONG | 2012 | Frontiers of Computer Science2012，6，4： | 5 |
| 4 | A short-chain α-neurotoxin from Naja naja atra produces potent cholinergic-dependent analgesia显示文摘Objective To investigate the analgesia induced by cobrotoxin (CT) from venom of Naja naja atra, and the effects of atropine and naloxone on the antinociceptive activity of CT in rodent pain models. Methods CT was administered intraperitoneally (33.3,50,7μg/kg) , intra-cerebral venticularly (2.4μg /kg) or microinjected into periaque-ductal gray (PAG,1.2μg /kg). The antinociceptive action was tested using the hot-plate test and the acetic acid writhing test in mice and rats. The involvement of cholinergic system and the opioid system in CT-induced analgesia was examined by pretreatment of animals with atropine (0.5mg/kg, im or 10mg/kg, ip) or naloxone (3mg/kg,ip) . The effect of CT on motor activity was tested using the Animex test. Results CT (33.3,50 and 75μg/kg,ip) exhibited a dose-dependent analgesic action in mice as determined with hot-plate test and acetic acid writhing test. In the mouse acetic acid writhing test, the intra-cerebral ventricle administration of CT 2.4μg/kg (1/23th of a systemic dose) produced marked analgesic effects. Microinjection of CT 1.2μg/kg (1/46th of systemic dose) into the PAG also elicited a robust analgesic action in the hot-plate test in rats. Atropine at 0.5 mg/kg (im) or naloxone at 3 mg/kg (ip) failed to block the analgesic effects of CT, but atropine at 10 mg/kg (ip) did antagonize the analgesia mediated by CT in the mouse acetic acid writhing test. At the highest effective dose of antinociception (75μg/kg) , CT did not change the spontaneous mobility of mice. Conclusion These results suggest that CT from Naja naja atra venom has analgesic effects. Central nervous system may be involved in CT' analgesic effects and the PAG may be the primary central site where CT exerts its effects. The central cholinergic system but not opioid system appears to be involved in the antinociceptive action of CT. | Hui-Ling ZHANG Rong HAN Zhen-Lun GU Zhi-Xing CHEN Bo-Wen CHEN Paul F. Reid Laurence N. Raymond Zheng-Hong QIN | 2006 | Neuroscience Bulletin2006，22，2： | 5 |
| 5 | ROR2 gene is associated with risk of non-syndromic cleft palate in an Asian population显示文摘背景受体酷氨酸象kinase一样孤儿受体 2 ( ROR2 )基因最近被显示了在动物模型在腭的开发起重要作用并且居住在连接到的 chromosomal 区域非, syndromic 劈开嘴唇与或没有在学习是调查可能的协会在之间的这的 humans.The 目的劈开腭 ROR2 基因和 non-syndromic 我们测试了的这里的口头的 clefts.Methods 在在 297 non-syndromic 的 ROR2 基因的 38 合格单个核苷酸的多型性( SNP ) | Wang Hong Hetmanski, Jacqueline B. Ruczinski, Ingo Liang, Kung Yee Fallin, M. Daniele Redett, Richard J. Raymond, Gerald V. Chou, Yah-Huei Wu Chen, Philip Kuo-Ting Yeow, Vincent Chong, Samuel S. Cheah, Felicia S. H. Jabs, Ethylin Wang Scott, Alan F. Beaty, Terri H. | 2012 | Chinese Medical Journal2012，，3： | 5 |
| 6 | Whole-genome sequencing of monozygotic twins discordant for schizophrenia indicates multiple genetic risk factors for schizophrenia显示文摘Schizophrenia is a common disorder with a high heritability, but its genetic architecture is still elusive.We implemented whole-genome sequencing(WGS) analysis of 8 families with monozygotic(MZ) twin pairs discordant for schizophrenia to assess potential association of de novo mutations(DNMs) or inherited variants with susceptibility to schizophrenia. Eight non-synonymous DNMs(including one splicing site) were identified and shared by twins, which were either located in previously reported schizophrenia risk genes(p.V24689 I mutation in TTN, p.S2506 T mutation in GCN1L1, IVS3+1G > T in DOCK1) or had a benign to damaging effect according to in silico prediction analysis. By searching the inherited rare damaging or loss-of-function(LOF) variants and common susceptible alleles from three classes of schizophrenia candidate genes, we were able to distill genetic alterations in several schizophrenia risk genes, including GAD1, PLXNA2, RELN and FEZ1. Four inherited copy number variations(CNVs; including a large deletion at 16p13.11) implicated for schizophrenia were identified in four families, respectively. Most of families carried both missense DNMs and inherited risk variants, which might suggest that DNMs, inherited rare damaging variants and common risk alleles together conferred to schizophrenia susceptibility. Our results support that schizophrenia is caused by a combination of multiple genetic factors, with each DNM/variant showing a relatively small effect size. | Jinsong Tang Yu Fan Hong Li Qun Xiang Deng-Feng Zhang Zongchang Li Ying He Yanhui Liao Ya Wang Fan He Fengyu Zhang Yin Yao Shugart Chunyu Liu Yanqing Tang Raymond C.K.Chan Chuan-Yue Wang Yong-Gang Yao Xiaogang Chen | 2017 | Journal of Genetics and Genomics2017，44，6： | 4 |
| 7 | Blockade of the swelling-induced chloride current attenuates the mouse neonatal hypoxic-ischemic brain injury in vivo显示文摘胀大导致 Cl 电流的激活(我 Cl,胀大 ) 在新生的组织缺氧局部缺血期间(嗨) 可以导致大脑损坏。Hypoxic-ischemic 大脑损害在出生不满一月的婴儿以及尖锐死亡引起长期的神经病学的病态。在这研究,我们调查了角色我 Cl,胀大在用选择 blocker 的 hypoxic-ischemic 大脑损害的 , 4-(2-butyl-6,7-dichloro-2-cyclopentylindan-1-on-5-yl ) oxybutyric 酸(DCPIB ) 。在主要有教养的外皮的神经原, 30% 张力减退的答案的灌注激活我 Cl,胀大是的 , 完全由 DCPIB (10 mol/L ) 的申请堵住了。角色我 Cl,胀大在在 vivo 的新生的 hypoxic-ischemic 大脑损害的 在一个修改新生的 hypoxic-ischemic 大脑损害模型被评估。在收到 ischemic 侮辱前,老鼠小狗与 DCPIB 被注射(10 mg/kg, ip ) 。我们发现有 DCPIB 的那个预告的处理显著地减少了用 TTC 染色, Nissl 染色和整个大脑成像估计的大脑损坏,并且改进了在 neurobehavioural 测试评估的感觉运动、门厅的恢复结果(即趋地性 reex,和峡谷回避 reex ) 。这些结果证明 DCPIB 在新生的 hypoxic-ischemic 大脑损害上有 neuroprotective 效果,并且 I Cl,胀大 可以为 hypoxic-ischemic encephalopathy 的处理用作一个治疗学的目标。 | Raymond WONG Ahmed ABUSSAUD Joseph WH LEUNG Bao-feng XU Fei-ya LI Sammen HUANG Nai-hong CHEN Guan-lei WANG Zhong-ping FENG Hong-shuo SUN | 2018 | Acta Pharmacologica Sinica2018，39，5： | 3 |
| 8 | Silvernanoparticles inhibit hepatitis B virus replication显示文摘 | Lu Lei Sun raymond wai-yin Chen Rong | 2008 | Antiviral Therapy2008，13，2： | 1 |
| 9 | Roles of rifampicin in drug-drug interactions:underlying molecular mechanisms involving the nuclear pregnane X receptor Ann Clin Microbiol Antimicrob显示文摘 | Chen JZ Raymond K | 2006 | Ann Clin Microbiol Antimicrob2006，15，3： | 1 |
| 10 | Differential Patterns of MicroRNA Expression in Neuroblastoma Are Correlated with Prognosis,Differentiation,and Apoptosis显示文摘 | Chen YX Raymond LS | 2007 | Cancer Res2007，67，3： | 1 |
| 11 | Are the Discounts on Closed-End Funds are a Sentiment Index显示文摘 | CHEN Nai-fu Raymond K Merton H Miller | 1993 | Journal of Finance1993，48，2： | 1 |
| 12 | Acceptability of five non- oxynol-9 spermicides显示文摘 | Raymond EG Chen PL Condon S | 2005 | Cont2005，71，： | 1 |
| 13 | Microbial communities involved in electricity generation from sulfide oxidation in a microbial fuel cell显示文摘 | Min Sun Zhong-Hua Tong Guo-Ping Sheng Yong-Zhen Chen Feng Zhang Zhe-Xuan Mu Hua-Lin Wang Raymond J. Zeng Xian-Wei Liu Han-Qing Yu Li Wei Fang Ma | 2010 | Biosensors and Bioelectronics2010，，2： | 1 |
| 14 | Localization of cerebral functional deficits in treatment-naive, first-episode schizophrenia using resting-state fMRI显示文摘 | Xiao-Qi Huang Su Lui Wei Deng Raymond C.K. Chan Qi-Zhu Wu Li-Jun Jiang Jun-Ran Zhang Zhi-Yun Jia Xiu-Li Li Fei Li Long Chen Tao Li Qi-Yong Gong | 2009 | Neuroimage2009，，： | 1 |
| 15 | Expression and Inheritance of Nine Transgenes in Rice显示文摘 | Liying Wu Somen Nandi Lifang Chen Raymond L. Rodriguez Ning Huang | 2002 | Transgenic Research2002，，5： | 1 |
| 16 | Trends in Prevalence of HIV, Syphilis, Hepatitis C, Hepatitis B, and Sexual Risk Behavior Among Men Who Have Sex With Men: Results of 3 Consecutive Respondent-Driven Sampling Surveys in Beijing, 2004 Through 2006显示文摘 | Xiaoyan Ma Qiyun Zhang Xiong He Weidong Sun Hai Yue Sanny Chen H Fisher Raymond Yang Li Min Xu Hui Du Willi McFarland | 2007 | JAIDS Journal of Acquired Immune Deficiency Syndromes2007，，： | 1 |
| 17 | Capability-based IoT access control using blockchain显示文摘Internet of Things(IoT)devices facilitate intelligent service delivery in a broad range of settings,such as smart offices,homes and cities.However,the existing IoT access control solutions are mainly based on conventional identity management schemes and use centralized architectures.There are knowm security and privacy limitations with such schemes and architectures,such as the single-point failure or surveillance(e.g.,device tracking).Hence,in this paper,we present an architecture for capability-based IoT access control utilizing the blockchain and decentralized identifiers to manage the identity and access control for IoT devices.Then,we propose a protocol to provide a systematic view of system interactions,to improve security.We also implement a proof-of-concept prototype of the proposed approach and evaluate the prototype using a real-world use case.Our evaluation results show that the proposed solution is feasible,secure,and scalable. | Yue Liu Qinghua Lu Shiping Chen Qiang Qu Hugo’Connor Kim-Kwang Raymond Choo He Zhang | 2021 | Digital Communications and Networks2021，7，4： | 1 |
| 18 | Beta - glucans in the treatment of diabetes and associated cardiovascular risks 显示文摘 | Chen J Raymond K | 2008 | Yasc Health Risk Manag2008，4，6： | 1 |
| 19 | Combination of bovine carbonic anhydrase with a fluorescent sulfonamide显示文摘 | John C Kernohan | 1967 | J Biol Chem1967，242，： | 1 |
| 20 | Garlic-Derived S-Allylmercaptocysteine Ameliorates Nonalcoholic Fatty Liver Disease in a Rat Model through Inhibition of Apoptosis and Enhancing Autophagy显示文摘 | Jia Xiao Rui Guo Man-Lung Fung Emily C. Liong Raymond Chuen Chung Chang Yick-Pang Ching George L. Tipoe Yueh-Sheng Chen | 2013 | <journal-title>Evidence-Based Complementary and Alternative Medicine2013，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员