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| 1 | Heavy metals contents in soil profiles of typical agricultural lands in Yixing,Jiangsu Province,China显示文摘The effect of land use pattern on the heavy metals(HMs)distribution in soil profiles was investigated in Yixing City,China,for the four typical types of land utilization,i.e.vegetable fields on the plain,paddy fields,tea gardens on hilly lands and bamboo groves.The variance of HMs concentration in the depth of 0-15 cm,15-30 cm,30-60 cm of soil profiles was compared among the different tillage ages. Results showed there was a notable accumulation of Cd,Hg,Cu,Pb,Zn and Cr in the soil profiles of vegetable fields and paddy fields causing a pollution of Cu,Hg and Cd in some profiles,while no remarkable accumulation of HMs in the soil profiles of the tea gardens and bamboo groves was found. The accumulation extent of HMs in the soil profiles of the four kinds of land use followed a sequence of vegetable fields>paddy fields>tea gardens>bamboo groves.The accumulation of HMs was mainly due to the industry,transportation,other urban human activities,and intensive use of fertilizers and pesticides.The downward leaching of HMs was relatively remarkable in the soil profiles of vegetable fields. There was an accumulation in surface and subsurface layers for Cd,Hg,Pb,Zn in the soil profiles of paddy fields and tea gardens,and for Cd,Hg in those of bamboo groves.Serious pollution effects of Hg, Pb,and Zn have been shown in the soil of vegetable fields.All the four kinds of agricultural land are basically free from the pollution of the external sources of As and Ni.With the increase of tillage time, the soil of vegetable and paddy fields will face the potential risk of HMs pollution,which mainly comes from Hg,Cd and Cu,whereas that of tea gardens and bamboo groves faces a relatively small risk. | Zhou ShengLu Liao FuQiang Wu ShaoHua Zhang HongFu Ren Kui | 2008 | Chinese Science Bulletin2008，53，S1： | 11 |
| 2 | Activating ZnO nanorod photoanodes in visible light by Cu ion implantation显示文摘 | Meng Wang Feng Ren Guangxu Cai Yichao Liu Shaohua Shen Liejin Guo | 2014 | Nano Research2014，7，3： | 9 |
| 3 | Cloning,expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug,Adelphocoris lineolatus(Goeze)显示文摘Credible evidence shows that odorant binding proteins(OBPs)are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors(ORs).In the present study,a novel OBP(AlinOBP3)gene from the lucerne plant bug,Adelphocoris lineolatus,was cloned and expressed.The expression pattern of AlinOBP3 was evaluated by qPCR,which indicated that AlinOBP3 was dominantly expressed in antennae.The binding properties of AlinOBP3 with 9 cotton volatiles and 5 sex pheromone analogs were measured by fluorescence competitive binding assays with the fluorescence probe 1-NPN.The results revealed that of 9 cotton volatiles,Myrcene,β-Ocimene and α-Phellandrene can bind with AlinOBP3.α-Phellandrene especially bound to AlinOBP3 with a high binding affinity,with a dissociation constant of 56.68 μmol/L.Of the 5 sex pheromone analogs,Hexyl butyrate had the strongest binding affinity with AlinOBP3,with a dissociation constant as 59.53 μmol/L.Butyl butyrate,trans-2-Hexenyl butyrate and Ethyl butyrate had medium binding affinities with AlinOBP3,with dissociation constants of 227.39,108.77 and 143.47 μmol/L,respectively.The results suggest that AlinOBP3 might be a pheromone binding protein(PBP)with a dual-function for the perception of sex pheromones and plant volatiles. | GU ShaoHua SUN Yang REN LiYan ZHANG XueYing ZHANG YongJun WU KongMing GUO YuYuan | 2010 | Chinese Science Bulletin2010，55，34： | 7 |
| 4 | Spatial distribution and sources of soil heavy metals in the outskirts of Yixing City,Jiangsu Province,China显示文摘City outskirts serve as the concentration centers for the pollutants discharged from various sources such as industry,agriculture and transportation.With the acceleration of industrialization and urbanization,the eco-environment of the city outskirts has become a hot spot of public concern.An analysis was conducted in this paper on the soil in the outskirts of Yixing City using frequency distribution functions.The heavy metal sources in this region had been divided into two components,i.e.natural background component and strong human disturbance component.The corresponding interpretation of the distribution pattern and features of heavy metal elements was presented by spatial analysis.The results showed that the strong human disturbance components of Hg,Pb,and Cu accounted for 36.9%, 26.7%,and 23.3%in their contents respectively,which indicated directly the serious effect of human activities on heavy metal contents.Hg and Pb,because of the human disturbance,showed the greatest spatial variability,and human activities intensified the heterogeneity of the spatial distribution.The anisotropic analysis showed the higher organisation of Hg and Zn in the direction of urban-rural transition,which indicated their spatial characteristics with urban-rural transition.Pb displayed distinctive structure in the vertical direction of urban-rural transition,which was largely controlled by highway distribution.Cu content in paddy fields was significantly higher than those of other land-use-types,and the agricultural non-point source pollution played an important role in the distribution pattern of Cu. | ShaoHua Wu ShengLu Zhou DeZhi Yang FuQiang Liao HongFu Zhang Kui Ren | 2008 | Chinese Science Bulletin2008，53，S1： | 7 |
| 5 | Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters显示文摘The efficacy of the CRISPR/Cas9 system in grapevine(Vitis vinifera L.)has been documented,but the optimization of this system,as well as CRISPR/Cas9-mediated multiplex genome editing,has not been explored in this species.Herein,we identified four VvU3 and VvU6 promoters and two ubiquitin(UBQ)promoters in grapevine and demonstrated that the use of the identified VvU3/U6 and UBQ2 promoters could significantly increase the editing efficiency in grape by improving the expression of sgRNA and Cas9,respectively.Furthermore,we conducted multiplex genome editing using the optimized CRISPR/Cas9 vector that contained the conventional multiple sgRNA expression cassettes or the polycistronic tRNA-sgRNA cassette(PTG)by targeting the sugar-related tonoplastic monosaccharide transporter(TMT)family members TMT1 and TMT2,and the overall editing efficiencies were higher than 10%.The simultaneous editing of TMT1 and TMT2 resulted in reduced sugar levels,which indicated the role of these two genes in sugar accumulation in grapes.Moreover,the activities of the VvU3,VvU6,and UBQ2 promoters in tobacco genome editing were demonstrated by editing the phytoene desaturase(PDS)gene in Nicotiana benthamiana leaves.Our study provides materials for the optimization of the CRISPR/Cas9 system.To our knowledge,our simultaneous editing of the grape TMT family genes TMT1 and TMT2 constitutes the first example of multiplex genome editing in grape.The multiplex editing systems described in this manuscript expand the toolbox of grape genome editing,which would facilitate basic research and molecular breeding in grapevine. | Chong Ren Yanfei Liu Yuchen Guo Wei Duan Peige Fan Shaohua Li Zhenchang Liang | 2021 | Horticulture Research2021，8，1： | 6 |
| 6 | A novel trajectory planning strategy for aircraft emergency landing using Gauss pseudospectral method显示文摘To improve the survivability during an emergency situation, an algorithm for aircraft forced landing trajectory planning is proposed. The method integrates damaged aircraft modelling and trajectory planning into an optimal control framework. In order to deal with the complex aircraft flight dynamics, a solving strategy based on Gauss pseudospetral method(GPM) is presented.A 3-DOF nonlinear mass-point model taking into account the wind is developed to approximate the aircraft flight dynamics after loss of thrust. The solution minimizes the forced landing duration, with respect to the constraints that translate the changed dynamics, flight envelope limitation and operational safety requirements. The GPM is used to convert the trajectory planning problem to a nonlinear programming problem(NLP), which is solved by sequential quadratic programming algorithm. Simulation results show that the proposed algorithm can generate the minimum-time forced landing trajectory in event of engine-out with high efficiency and precision. | Shaohua MENG Jinwu XIANG Zhangping LUO Yiru REN Nanjian ZHUANG | 2014 | Control Theory and Technology2014，12，4： | 2 |
| 7 | External beam radiation therapy is safe and effective in treating primary pulmonary amyloidosis 显示文摘 | Shaohua Ren Gang Ren | 2012 | Respiratory Medicine2012，106，： | 1 |
| 8 | Preparation and characterization of some surface negatively charged residue mutants of cytochrome b_5显示文摘Site-directed mutagenesis was used to obtain seven variants of tryptic fragment of bovine liver cytochrome bs (cyt bs), in which the negatively charged residues around the heme exposed edge of cyt bs were replaced by hydropho-bic amino acid alanine. Double-site mutants, triple-site mutants and even quadruple-site mutants were obtained. DNA sequencing and molecular weight measurements of the mutant proteins both confirmed that these site-directed muta-genesises were successfully performed. Spectroelectrochem-istry of these mutant proteins revealed that the apparent redox potentials of these mutant proteins caused a positive shift of 2-10 mV. The global structure of these mutant proteins did not show much difference from that of the wild type cyt bs, providing a solid base for the further study on the roles of the proteins’ surface charges. | WANG Yunhua, WANG Wenhu, LU Junxia, REN Yi, GU Shaohua, XIE Yi & HUANG Zhongxian1. Chemical Biology Lab, Department of Chemistry, Fudan University, Shanghai 200433, China 2. Genetic Institute, School of Life, Fudan University, Shanghai 200433, China | 2001 | Chinese Science Bulletin2001，46，7： | 1 |
| 9 | Solar light-driven photocatalytic hydrogen evolution over ZnIn2S4 loaded with transition-metal sulfides显示文摘 | Shaohua Shen Xiaobo Chen Feng Ren Coleman X Kronawitter Samuel S Mao Liejin Guo | 2011 | Nanoscale Research Letters2011，，1： | 1 |
| 10 | Enhanced electrochemical performance of Li-rich cathode Li[Li 0.2 Mn 0.54 Ni 0.13 Co 0.13 ]O 2 by surface modification with lithium ion conductor Li 3 PO 4显示文摘 | Zhiyuan Wang Shaohua Luo Jie Ren Dan Wang Xiwei Qi | 2016 | Applied Surface Science2016，，： | 1 |
| 11 | A stochas-tic -coverage scheduling algorithm in wireless sensor net-works 显示文摘 | Yu Jiguo Ren Shaohua Wan Shengli | 2012 | International Journal of Distributed SensorNetworks2012，33，5： | 1 |
| 12 | Intrapleural staphylococcal superantigen induces resolution of malignant pleural effusions and a survival benefit in non-small cell lung cancer显示文摘 | Shaohua Ren | 2004 | Chest2004，126，5： | 1 |
| 13 | VvBBX44 and VvMYBA1 form a regulatory feedback loop to balance anthocyanin biosynthesis in grape显示文摘Anthocyanins are essential for the quality of perennial horticultural crops,such as grapes.In grapes,ELONGATED HYPOCOTYL 5(HY5)and MYBA1 are two critical transcription factors that regulate anthocyanin biosynthesis.Our previous work has shown that Vitis vinifera B-box protein 44(VvBBX44)inhibits anthocyanin synthesis and represses VvHY5 expression in grape calli.However,the regulatory mechanism underlying this regulation was unclear.In this study,we found that loss of VvBBX44 function resulted in increased anthocyanin accumulation in grapevine callus.VvBBX44 directly represses VvMYBA1,which activates VvBBX44.VvMYBA1,but not VvBBX44,directly modulates the expression of grape UDP flavonoid 3-O-glucosyltransferase(VvUFGT).We demonstrated that VvBBX44 represses the transcriptional activation of VvUFGT and VvBBX44 induced by VvMYBA1.However,VvBBX44 and VvMYBA1 did not physically interact in yeast.The application of exogenous anthocyanin stimulated VvBBX44 expression in grapevine suspension cells and tobacco leaves.These findings suggest that VvBBX44 and VvMYBA1 form a transcriptional feedback loop to prevent overaccumulation of anthocyanin and reduce metabolic costs.Our work sheds light on the complex regulatory network that controls anthocyanin biosynthesis in grapevine. | Wenwen Liu Huayuan Mu Ling Yuan Yang Li Yuting Li Shenchang Li Chong Ren Wei Duan Peige Fan Zhanwu Dai Yongfeng Zhou Zhenchang Liang Shaohua Li Lijun Wang | 2023 | Horticulture Research2023，10，10： | 0 |
| 14 | Highly efficient and anti-poisoning single-atom cobalt catalyst for selective hydrogenation of nitroarenes显示文摘Developing non-precious metal catalysts to selectively reduce functionalized nitroarenes with high efficiency is urgently desirable for the production of value-added amines.Herein,we report a novel,efficient,anti-poisoning single-atom cobalt catalyst(Co-NAC)for the highly selective hydrogenation of the nitro to amino group for nitroarenes baring various functional groups,including vinyl,cyano,and halogen.Using a combination of structure characterization techniques,we have confirmed that the cobalt species are predominantly present in the form of four-coordinated Co single sites anchored on nitrogen-assembly carbon(NAC)as the ordered mesoporous support.Co-NAC catalysts enable the full conversion and>99%selectivity with molecular H2 as a green reductant under mild conditions(80℃,2 MPa H2).As for the selective hydrogenation of 3-nitrostyrene,Co-NAC catalyst affords high catalytic productivity(19.7 h-1),which is superior to the cobalt nanoparticles(NPs)catalysts and most of the recently reported Co-based catalysts.This is attributed to the highly accessible atomically-dispersed Co active sites,the high surface area with ordered-mesoporous morphology and the prominent high content of nitrogen dopants.Notably,Co-NAC catalyst displays resistance towards sulfur-containing poisons(20 equivalents)and strong non-oxidizing acid(8 M),showing great potential for continuous application in the chemical industry. | Yuemin Lin Renfeng Nie Yuting Li Xun Wu Jiaqi Yu Shaohua Xie Yajing Shen Shanjun Mao Yuzhuo Chen Dan Lu Zongbi Bao Qiwei Yang Qilong Ren Yiwen Yang Fudong Liu Long Qi Wenyu Huang Zhiguo Zhang | 2022 | Nano Research2022，15，12： | 0 |
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