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| 1 | The role of gut microbiota(commensal bacteria)and the mucosal barrier in the pathogenesis of inflammatory and autoimmune diseases and cancer:contribution of germ-free and gnotobiotic animal models of human diseases显示文摘Metagenomic approaches are currently being used to decipher the genome of the microbiota(microbiome),and,in parallel,functional studies are being performed to analyze the effects of the microbiota on the host.Gnotobiological methods are an indispensable tool for studying the consequences of bacterial colonization.Animals used as models of human diseases can be maintained in sterile conditions(isolators used for germ-free rearing)and specifically colonized with defined microbes(including non-cultivable commensal bacteria).The effects of the germ-free state or the effects of colonization on disease initiation and maintenance can be observed in these models.Using this approach we demonstrated direct involvement of components of the microbiota in chronic intestinal inflammation and development of colonic neoplasia(i.e.,using models of human inflammatory bowel disease and colorectal carcinoma).In contrast,a protective effect of microbiota colonization was demonstrated for the development of autoimmune diabetes in non-obese diabetic(NOD)mice.Interestingly,the development of atherosclerosis in germ-free apolipoprotein E(ApoE)-deficient mice fed by a standard low-cholesterol diet is accelerated compared with conventionally reared animals.Mucosal induction of tolerance to allergen Bet v1 was not influenced by the presence or absence of microbiota.Identification of components of the microbiota and elucidation of the molecular mechanisms of their action in inducing pathological changes or exerting beneficial,disease-protective activities could aid in our ability to influence the composition of the microbiota and to find bacterial strains and components(e.g.,probiotics and prebiotics)whose administration may aid in disease prevention and treatment. | Helena Tlaskalova-Hogenova Renata Stepankova Hana Kozakova Tomas Hudcovic Luca Vannucci Ludmila Tuckova Pavel Rossmann TomasHrncır Miloslav Kverka Zuzana Zakostelska Klara Klimesova Jaroslava Pribylova Jirina Bartova Daniel Sanchez Petra Fundova Dana Borovska Dagmar Sru˚tkova Zdenek Zıdek Martin Schwarzer Pavel Drastich David P Funda | 2011 | Cellular & Molecular Immunology2011，8，2： | 47 |
| 2 | Protective action of glutamine in rats with severe acute liver failure显示文摘BACKGROUND Severe acute liver failure(SALF) is a rare, but high-mortality, rapidly evolving syndrome that leads to hepatocyte degeneration with impaired liver function.Thioacetamide(TAA) is a known xenobiotic, which promotes the increase of the formation of reactive oxygen species. Erythroid 2-related factor 2(Nrf2) activates the antioxidant protection of cells. Studies have evidenced the involvement of inflammatory mediators in conditions of oxidative stress.AIM To evaluate the antioxidant effects of glutamine on Nrf2 activation and NFκBmediated inflammation in rats with TAA-induced IHAG.METHODS Male Wistar rats(n = 28) were divided into four groups: control,control+glutamine, TAA, and TAA + glutamine. Two TAA doses(400 mg/kg)were administered intraperitoneally, 8 h apart. Glutamine(25 mg/kg) was administered at 30 min, 24 h, and 36 h. At 48 h, blood was collected for liver integrity analysis [aspartate aminotransferase(AST), alanine aminotransferase(ALT), and alkaline phosphatase(ALP)]. The liver was harvested for histology and assessment of oxidative stress [thiobarbituric acid-reactive substances(TBARS), catalase(CAT), glutathione peroxidase(GPx), glutathione S-transferase(GST), glutathione(GSH), Nrf2, Kelch-like ECH-associated protein 1(Keap1),NADPH quinone oxidoreductase1(NQO1), superoxide dismutase(SOD)] and inflammatory process.RESULTS TAA caused disruption of the hepatic parenchyma, with inflammatoryinfiltration, massive necrosis, and ballooning degeneration. Glutamine mitigated this tissue damage, with visible regeneration of hepatic parenchyma; decreased TBARS(P < 0.001), GSH(P < 0.01), IL-1β, IL6, and TNFα levels(P <0.01) in hepatic tissue; and decreased blood levels of AST, ALT, and ALP(P <0.05). In addition, CAT, GPx, and GST activities were restored in the glutamine group(P<0.01, P <0.01, and P <0.001, respectively vs TAA alone). Glutamine increased expression of Nrf2(P < 0.05), NQO1, and SOD(P < 0.01), as well as levels of IL-10(P <0.001), while decreasing expression of Keap1, TLR4, NFκB(P < 0.001), COX-2 and iNOS,(P < 0.01), and reducing NO_2 and NO_3 levels(P < 0.05).CONCLUSION In the TAA experimental model of IHAG, glutamine activated the Nrf2 pathway,thus promoting antioxidant protection, and blunted the NFκB-mediated pathway, reducing inflammation. | Elizangela G Schemitt Renata M Hartmann Josieli R Colares Francielli Licks Jéferson O Salvi Cláudio A Marroni Norma P Marroni | 2019 | World Journal of Hepatology2019，11，3： | 5 |
| 3 | Significance of postoperative follow-up of patients with metastatic colorectal cancer using circulating tumor DNA显示文摘BACKGROUND One of the most notable applications for circulating tumor DNA(ctDNA)detection in peripheral blood of patients with metastatic colorectal cancer(mCRC)is a long-term postoperative follow-up.Sometimes referred to as a“liquid(re)biopsy”it is a minimally invasive procedure and can be performed repeatedly at relatively short intervals(months or even weeks).The presence of the disease and the actual extent of the tumor burden(tumor mass)within the patient’s body can be monitored.This is of particular importance,especially when evaluating radicality of surgical treatment as well as for early detection of disease progression or recurrence.AIM To confirm the radicality of surgery using ctDNA and compare available methods for detection of recurrence in metastatic colorectal cancer.METHODSA total of 47 patients with detected ctDNA and indications for resection of mCRC were enrolled in the multicenter study involving three surgical centers.Standard postoperative follow-ups using imaging techniques and the determination of tumor markers were supplemented by ctDNA sampling.In addition to the baseline ctDNA testing prior to surgery,a postoperative observation was conducted by evaluating ctDNA presence up to a week after surgery and subsequently at approximately three-month intervals.The presence of ctDNA was correlated with radicality of surgical treatment and the actual clinical status of the patient.RESULTS Among the monitored patients,the R0(curative)resection correlated with postoperative ctDNA negativity in 26 out of 28 cases of surgical procedures(26/28,93%).In the remaining cases of R0 surgeries that displayed ctDNA,both patients were diagnosed with a recurrence of the disease after 6 months.In 7 patients who underwent an R1 resection,4 ctDNA positivities(4/7,57%)were detected after surgery and associated with the confirmation of early disease recurrence(after 3 to 7 months).All 15 patients(15/15,100%)undergoing R2 resection remained constantly ctDNA positive during the entire follow-up period.In 22 cases of recurrence,ctDNA positivity was detected 22 times(22/22,100%)compared to 16 positives(16/22,73%)by imaging methods and 15 cases(15/22,68%)of elevated tumor markers.CONCLUSION ctDNA detection in patients with mCRC is a viable tool for early detection of disease recurrence as well as for confirmation of the radicality of surgical treatment. | Lucie Benešová Tereza Hálková Renata Ptáčková Anastasiya Semyakina Kateřina Menclová JiříPudil Miroslav Ryska Miroslav Levý JaromírŠimša Filip Pazdírek JiříHoch Milan Blaha Marek Minárik | 2019 | World Journal of Gastroenterology2019，25，48： | 3 |
| 4 | Degradation of chitosan and starch by 360kHz ultrasound显示文摘 | Renata C B Bozena R Salah L | 2005 | Carbohydrate Polymers2005，60，2： | 1 |
| 5 | The nature of the silicacage as reflected by spectral changes and enhanced photostability oftrapped rhodamine 6G 显示文摘 | David A David L Renata R | 1984 | J Phys Chem1984，88，： | 1 |
| 6 | Viral RNAi suppressor reversibly binds siRNA to outcompete dicer and RISC via multiple turnover 显示文摘 | Renata A R Vishalakshi K Nils G W | 2011 | J Mol Biol2011，408，2： | 1 |
| 7 | Electromagnetic Stimulation Combined with Aloe vera Increases Collagen Reorganization in Burn Repair显示文摘 | Sofia Poletti Leticia D. Lucke Fernanda O Bortolazzo Renata M. Acunha Marcos G. Mattos Femanda OG. Gaspi Thiago A. M. Andrade Maria Esmeria C. Amaral Andrea A .Aro Edson R Pimentel Marcelo AM. Esquisatto Fernanda A. S. Mendonga Glaucia M. T. Santos | 2018 | Journal of Pharmacy and Pharmacology2018，6，7： | 1 |
| 8 | Influence of agglomeration of cerium oxide nanoparticles and speciation of cerium(III) on short term effects to the green algae Chlamydomonas reinhardtii显示文摘 | Lena A. R?hder Tanja Brandt Laura Sigg Renata Behra | 2014 | Aquatic Toxicology2014，，： | 1 |
| 9 | Degradation of chitosan and starch by 360 kHz ultrasound显示文摘 | Renata C B Bozena R Salah L | 2005 | Carbohydrate Polymers2005，60，2： | 1 |
| 10 | Influence of emulsion composition and inlet air temperature on the microencapsulation of flaxseed oil by spray drying显示文摘 | Renata V Tonon Carlos R F Grosso Miriam D Hubinger | | 0，，01： | 1 |
| 11 | Bone morphogenetic protein-4 reguldates its own expression in cultured osteoblasts显示文摘 | Renata CP Sheih R Emezto C | 2000 | J Cell Physiol2000，182，： | 1 |
| 12 | Social support and the ability to adapt to life among Brazilian street children and non-street children显示文摘 | RENATA CD' ABREU ANN K MULLIS LAURA R COOK | 2001 | The Journal of Social Psychology2001，141，1： | 1 |
| 13 | Influence of emulsion composition and inlet air temperature on the microencapsulation of flaxseed oil by spray drying 显示文摘 | Renata V Tonon Carlos R F Grosso Miriam D Hubinger | 2011 | Food Research International2011，，1： | 1 |
| 14 | Correlation between c-Jun and hu-man papillomavirus in oral premalignant and malignant lesions显示文摘 | Renata R A Elisa D S | 2008 | Oral Oncology2008，44，： | 1 |
| 15 | Degradatinon of chitosan and starch by 360 kHz ultrasound 显示文摘 | Renata C B Bozena R Salah L | 2005 | Carbohydrate Polymers2005，60，2： | 1 |
| 16 | Retrospective analysis of 25 women with malignant cystosarcoma phyllodes—treatment results显示文摘 | Renata Soumarová Zuzana ?eneklová Hana Horová Hana Vojkovská Ivana Horová Marie Budíková Jana R??i?ková Barbara Je?ková | 2004 | Archives of Gynecology and Obstetrics2004，，4： | 1 |
| 17 | Testing of microencapsulated flavours by electronic nose and SPME-GC显示文摘 | RENATA B PETRAS R V ALGIRDAS G | | 0，，01： | 1 |
| 18 | Innovative materials based on sol-gel technology显示文摘 | Renata R Tsiala S | 2006 | Optical Materials2006，28，6： | 1 |
| 19 | Production, characterizationand foamability of a -lactalbumin/glycomacropeptide supramolecularstructures显示文摘 | RENATA S D JANE S D R C | 2014 | Food Research International2014，64，： | 1 |
| 20 | Noninvasive in vivo quatitative assessment of fat conent in human liver显示文摘 | Renata L Eugenio G | 1997 | J Hepatol1997，27，1： | 1 |
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