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| 1 | The Blue Light-Dependent Phosphorylation of the CCE Domain Determines the Photosensitivity of Arabidopsis CRY2显示文摘CRY2 的 C 终端尾巴的蓝轻依赖者的 Phosphorylation。 | Qin Wang William D. Barshop Mingdi Bian Ajay A. Vashisht Reqing He Xuhong Yu Bin Liu Paula Nguyen Xuanming Liu Xiaoying Zhao James A. Wohlschlegel Chentao Lin | 2015 | Molecular Plant2015，8，4： | 3 |
| 2 | The long non-coding RNA DANA2 positively regulates drought tolerance by recruitingERF84 to promote JMJ29-mediated histone demethylation显示文摘Tens of thousands of long non-coding RNAs have been uncovered in plants,but few of them have been comprehensively studied for their biological function and molecular mechanism of their mode of action.Here,we show that the Arabidopsis long non-coding RNA DANA2 interacts with an AP2/ERF transcription factor ERF84 in the cell nucleus and then affects the transcription of JMJ29 that encodes a Jumonji C domain-containing histone H3K9 demethylase.Both RNA sequencing(RNA-seq)and genetic analyses demonstrate that DANA2 positively regulates drought stress responses through JMJ29.JMJ29 positively regulates the expression of ERF15 and GOLS2 by modulation of H3K9me2 demethylation.Accordingly,mutation of JMJ29 causes decreased ERF15 and GOLS2 expression,resulting in impaired drought tolerance,in agreement with drought-sensitive phenotypes of dana2 and erf84 mutants.Taken together,these results demonstrate that DANA2 is a positive regulator of drought response and works jointly with the transcriptional activator ERF84 to modulate JMJ29 expression in plant response to drought. | Pengxiang Zhang Reqing He Jun Yang Jingjing Cai Zhipeng Qu Rongxin Yang Jinbao Gu Zhen-Yu Wang David LAdelson Youlin Zhu Xiaofeng Cao Dong Wang | 2023 | Molecular Plant2023，16，8： | 0 |
| 3 | Expanding the range of CRISPR/Cas9-directed genome editing in soybean显示文摘The CRISPR/Cas9 system has been widely applied for plant genome editing.The commonly used SpCas9 has been shown to rely on the protospacer adjacent motif(PAM)sequences in the canonical form NGG and non-canonical NAG.Although these PAM sequences are extensively distributed across plant genomes,a broader scope of PAM sequence is required to expand the range of genome editing.Here we report the adoption of three variant enzymes,xCas9,SpCas9-NG and XNG-Cas9,to produce targeted mutation in soybean.Sequencing results determined that xCas9 with the NGG and KGA(contains TGA and GGA)PAMs successfully induces genome editing in soybean genome.SpCas9-NG could recognize NGD(contains NGG,NGA and NGT),RGC(contains AGC and GGC),GAA and GAT PAM sites.In addition,XNG-Cas9 was observed to cleave soybean genomic regions with NGG,GAA and AGY(contains AGC and AGT)PAM.Moreover,off-target analyses on soybean editing events induced by SpCas9 and xCas9 indicated that two high-fidelity Cas9 variants including eSpCas9(enhanced specificity SpCas9)and exCas9(enhanced specificity xCas9)could improve the specificity of the GGA PAM sequence without reducing on-target editing ficiency.These findings significantly expand the scope of Cas9-mediated genome editing in soybean. | Reqing He Pengxiang Zhang Yuchuan Yan Chen Yu Liyun Jiang Youlin Zhu Dong Wang | 2022 | aBIOTECH2022，3，2： | 0 |
| 4 | Manipulation of plant height in garden asparagus (Asparagus officinalis L.) through CRISPR/Cas9-mediated aspSPL14 allele editing显示文摘Dear Editor,Reduction in plant height has been associated with yield increases and yield stability in a number of important crop species,such as wheat and rice[1].In these plants,dwarfing is mainly attributed to the inability to synthesize or respond to certain phytohormones,predominantly gibberellin(GA)[2].Ideal Plant Architecture 1(IPA1),an miR156 target gene,encodes SPL14 and it is able to bind directly to the promoters of multiple GA biosynthetic,signal,and deactivating genes in rice[3].Moreover,IPA1 loss-of-function mutants exhibit dwarf phenotypes[4]. | Jingsong Zhou Reqing He Xiaojing Liu Bingbing Zhang Guangyu Chen Dong Wang Youlin Zhu | 2023 | Horticulture Research2023，10，7： | 0 |
| 5 | Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants显示文摘In this study,we constructed dual-transgene vectors(pDT1,pDT7,and pDT7G) that simultaneously co-expressed two genes in plants.ACTIN2 and UBQ10 promoters were used to control the expression of these two genes.The 4×Myc.3×HA,and 3×Flag reporter genes allowed for the convenient identification of a tunable co-expression system in plants,whereas the dexamethasone(Dex) inducible reporter gene C-terminus of the glucocorticoid receptor(cGR) provided Dex-dependent translocation of the fusion gene between the nucleus and cytoplasm.The function of pDT vectors was validated using four pairwise genes in Nicotiana benthamiana or Anihidopsis thaliana.The co-expression efficiency of two genes from the pDT1 and pDT7 G vectors was 35%and 42%,respectively,which ensured the generation of sufficient transgenic materials.These pDT vectors are simple,reliable,efficient,and time-saving tools for the co-expression of two genes through a single transformation event and can be used in the study of protein-protein interactions or multi-component complexes. | Zhimin He Bin Liu Xu Wang Mingdi Bian Reqing He Jindong Yan Ming Zhong Xiaoying Zhao Xuanming Liu | 2016 | Journal of Genetics and Genomics2016，43，4： | 0 |
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