| 6 | Kinin B_2 receptor does not exert renoprotective effects on mice with glycerol-induced rhabdomyolysis显示文摘AIM:To investigate a potential protective role of the kinin B2receptor in a glycerol-induced rhabdomyolysis mouse model.METHODS:We separated 28 C57Bl/6 male mice into4 groups:untreated WT animals,untreated B2knockout mice,glycerol-treated WT and glycerol-treated B2knockout mice.Glycerol-treated animals received one intramuscular injections of glycerol solution(50%v/v,7 m L/kg).After 48 h,urine and blood samples were collected to measure creatinine and urea levels.Additionally,kidney samples were extracted for histological evaluation,and the m RNA expression levels of kinin B1 and B2 receptors and inflammatory mediators were measured by real-time polymerase chain reaction.RESULTS:Serum creatinine and urea levels showed differences between untreated wild-type and glyceroltreated wild-type mice(0.66±0.04 vs 2.61±0.53mg/d L,P<0.01;and 33.51±2.08 vs 330.2±77.7mg/d L,P<0.005),and between untreated B2knockout mice and glycerol-treated knockout mice(0.56±0.03 vs 2.23±0.87 mg/d L,P<0.05;and 42.49±3.2vs 327.2±58.4 mg/d L,P<0.01),but there was no difference between the glycerol-treated wild-type and glycerol-treated knockout mice.Glycerol was able to induce a striking increase in kinin B2receptor expression(>30 times,31.34±8.9)in kidney.Animals injected with glycerol had a higher degree of tubular injury than untreated animals.Wild-type and knockout mice treated with glycerol intramuscularly present kidney injury,with impairment in renal function.However,B2knockout mice treated with glycerol did not show a different phenotype regarding kidney injury markers,when compared to the wild-type glycerol-treated group.CONCLUSION:We conclude that the kinin B2receptordoes not have a protective role in renal injury. | Pedro Paulo Gattai Fernando Francisco Pazello Mafra Frederick Wasinski Sandro Soares Almeida Marcos Ant?nio Cenedeze Denise Maria Avancini Costa Malheiros Reury Frank Pereira Bacurau Carlos Castilho Barros Niels Olsen Saraiva Camara Ronaldo Carvalho Araujo | 2014 | World Journal of Nephrology2014，3，3： | 0 |
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