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| 1 | Molecular mechanism of inbreeding effects based on RNA-Seq analysis of the adductor muscle of bay scallop(Argopecten irradians)显示文摘Understanding the effects of inbreeding is critical for the long-term viability of shellfish breeding and for understanding inbreeding depression(ID) of the offspring of selfed individuals and full-sib crosses. The bay scallop(Argopecten irradians) is a simultaneous hermaphroditic marine bivalve with distinct male and female gonads and is a species with external fertilization. It is a canonical model invertebrate for analysis of the genetics of ID and evolution of outcrossing mating system. ID in bivalves is well documented with regard to the phenotype and molecular markers aspects, but little is known on how inbreeding affects genome-wide gene expression.Here, we compare the gene expression profiles of adductor muscle of A. irradians lines inbred to F=0.5 with those of its counterpart outbred lines which are originated from the same paternal individual. Inbred and outbred individuals have some differences in growth traits. The shell length and the total weight of inbred scallops are lower than the counterpart of outbred lines. We found that 1 175 genes were up-regulated while 1 390 were downregulated in inbred lines. Regarding components, differentially expressed genes(DEGs) were concentrated on sarcomeres, contractile filaments, and cell membranes. Among others, whereas regarding their function, the Gene Ontology(GO) was concentrated on transferase, phosphotransferase, peptidase, and others. The DEGsenriched metabolic pathways mainly belonged to the oxidative phosphorylation pathway and apoptosis pathway.We used another two families to verify our bioinformatic results. The selfed and the outbred family originated from different ancestors, but the selfed family showed obvious ID. By using real-time quantitative reverse transcriptase polymerase chain reaction(qRT-PCR), we verified that DEGs with inbreeding are involved in the oxidative phosphorylation and cell apoptosis pathways, suggesting that inbreeding may affect energy metabolism and homeostasis. | Yu Wang Shoudu Zhang Guofan Zhang Rihao Cong Li Li | 2019 | Acta Oceanologica Sinica2019，38，2： | 0 |
| 2 | Polymorphism in the Ras andβ-Glucosidase Genes and Their Association with Growth Traits in the Pacific Oyster Crassostrea gigas显示文摘The Ras gene,a conserved member of the insulin pathway,andβ-glucosidase gene,an important cellulase,are two important growth-related genes.However,there is no study on the association between mutations of these two genes and growth traits in bivalves.Here,the polymorphism of these two genes in Crassostrea gigas were revealed.Their association with growth traits was evaluated in 290 oysters from five families,and was further confirmed in another 186 oysters from three fast-growing strains.Seventeen and twelve SNPs were identified in the Ras gene andβ-glucosidase gene,respectively.Among these SNPs,four SNPs in each gene(Ras:C.86C>A,C.90T>C,C.112A>G and C.118G>A;β-glucosidase:C.247G>A,C.284C>T,C.1260C>T and C.1293T>C)were significantly(P<0.05)associated with the growth of these oysters.Furthermore,eight and nine haplotypes were constructed in the Ras gene andβ-glucosidase gene,respectively.Oysters with both haplotypes R-Hap5(CCAA)andβ-Hap7(ACCT),or with both R-Hap 6(ATGG)andβ-Hap 6(ACTC),or with both R-Hap 6 andβ-Hap 9(ACTT),or with both R-Hap 7(ATAA)andβ-Hap 7,showed the highest growth performances.These results provide candidate markers for selecting C.gigas with fast growth. | HAN Ziqiang CONG Rihao LI Qi | 2021 | Journal of Ocean University of China2021，20，6： | 0 |
| 3 | Shell characterization and effects on cavity volume of wild Jinjiang oyster Crassostrea ariakensis in different estuaries of China显示文摘Jinjiang oyster Crassostrea ariakensis,a species with economic and ecological value,is distributed along the estuaries and coasts of East Asia.With the decline in natural resources,the conservation and aquaculture of this species is urgent.However,studies characterizing their shell shape remain scarce.We investigated the morphological differences in the shells of wild Jinjiang oysters from six populations(Qinzhou,Shanghai,Nantong,Qingdao,and Binzhou hard or muddy bottom)along the coast of China.The color of the shell and adductor muscle scar showed associations with temperature gradient along its geographical distribution.Oyster shape was defined by shell height to shell length ratio,and the ratio varies among geographic locations of the populations.They were found nearly round(Qinzhou and Nantong populations),oval(Qingdao and Binzhou populations),or water-droplet-shaped(Shanghai population).Binzhou populations living on muddy substrates are more elongated than those on hard substrate.In addition,we developed a method to measure the cavity volume in oysters.Correlation and path analysis showed that shell height significantly influenced cavity volume.The synergistic effect of the two factors(the height,length,and width of the shell in pairs)on the cavity volume resulted in differences between northern and southern groups:samples from the southern group(Qinzhou and Shanghai)showed correlation between shell height and shell width,while those from the northern group(Nantong,Qingdao,and Binzhou)showed correlation between shell height and shell length.All populations showed significant correlation between shell height and cavity height,and shell length and cavity length,while the correlation between shell width and cavity width was minimal,which may have been resulted from uneven shell thickness.The linear equation for shell height and cavity volume under different ratios of shell height to length was obtained.In this study,we determined that shell height has the most influence on cavity volume,and specific cavity volume fitting linear equations are given for different shell types,which may provide a reference for future oyster breeding for shell shaping. | Qi YANG Ao LI Luping WANG Rihao CONG Jianming YANG Guofan ZHANG Wei WANG Li LI | 2023 | Journal of Oceanology and Limnology2023，41，5： | 0 |
| 4 | Near-infrared spectroscopy method for rapid proximate quantitative analysis of nutrient composition in Pacific oyster Crassostrea gigas显示文摘Glycogen,amino acids,fatty acids,and other nutrient components affect the flavor and nutritional quality of oysters.Methods based on near-infrared reflectance spectroscopy(NIRS)were developed to rapidly and proximately determine the nutrient content of the Pacific oyster Crassostreagigas.Samples of C.gigas from 19 costal sites were freeze-dried,ground,and scanned for spectral data collection using a Fourier transform NIR spectrometer(Thermo Fisher Scientific).NIRS models of glycogen and other nutrients were established using partial least squares,multiplication scattering correction first-order derivation,and Norris smoothing.The R_(C) values of the glycogen,fatty acids,amino acids,and taurine NIRS models were 0.9678,0.9312,0.9132,and 0.8928,respectively,and the residual prediction deviation(RPD)values of these components were 3.15,2.16,3.11,and 1.59,respectively,indicating a high correlation between the predicted and observed values,and that the models can be used in practice.The models were used to evaluate the nutrient compositions of 1278 oyster samples.Glycogen content was found to be positively correlated with fatty acids and negatively correlated with amino acids.The glycogen,amino acid,and taurine levels of C.gigas cultured in the subtidal and intertidal zones were also significantly different.This study suggests that C.gigas NIRS models can be a cost-effective alternative to traditional methods for the rapid and proximate analysis of various slaughter traits and may also contribute to future genetic and breeding-related studies in Pacific oysters. | Zhe LI Haigang QI Ying YU Cong LIU Rihao CONG Li LI Guofan ZHANG | 2023 | Journal of Oceanology and Limnology2023，41，1： | 0 |
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