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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Deciphering the biology of My cobacterium tuberculosis from the complete genome sequence 显示文摘 | Cole S T Brosch R Parkhill J Gamier T Churcher C Harris D Gordon S V Eiglmeier K Gas S Barry C E Tekaia F Badcock K Basham D Brown D Chillingworth T Cormor R Davies R Devlin K FeltweU T Gentles S Hamlin N Holroyd S Hornsby T Jagels K Kroghs A McLean J Moule S Murphy L Oliver K Osborne J Quail M A Rafandream M A Rogers J Rutter S Seeger K Skelton J Squaraes R Squares S Sulston J E Taylo K Whitehead S Barrell B G | 1998 | Nature1998，393，： | 1 |
| 3 | An optimal multiedge detector for SAR image segment显示文摘 | ROGER F ARMAND L PHII IPPE M | 1988 | IEEE Trans actions on Geoscience and Re note Sensing1988，36，3： | 1 |
| 4 | C-peptide stimulates Na+ ,K+ ATPase activity via PKC alpha in rat medullary thick ascending limb显示文摘 | Tsimaratos M Roger F Chabardes D | 2003 | Diabetologia2003，46，1： | 1 |
| 5 | Characteristic Impedance De-termination Using Propagation Constant Measurement显示文摘 | Roger B M Dylan F W | 1991 | IEEE Microwave and Guided Wave Letters1991，，1： | 1 |
| 6 | C -peptide stimulates Na^+-K^+-ATPase activity via PKC alpha in rat medullary thick ascending limb显示文摘 | Tsimaratos M Roger F Chabardes D | 2003 | Diabetologia2003，46，1： | 1 |
| 7 | Musa-based cropping systems of the Cameroon highlands:a case study of the West and North West provinces of Cameroon,with emphasis on nematodes显示文摘 | Kim J Roger F Hugues M | 2004 | Fruits2004，59，： | 1 |
| 8 | Biodegradation K inetics of Benzene,Toluene,and Phenol as Single and Mixed Substrates for Pseudomonas putida F1显示文摘 | Reardon K F M osteller D C Rogers J | 2000 | Biotechnol Bioeng2000，69，4： | 1 |
| 9 | Solid-phase microextraction (SPME) thechnique for measurement of generation of fresh cucumber flavor compounds 显示文摘 | Charyl P H Roger F M F Herry P F | 2001 | Agi- culture and Food Chemistry2001，49，： | 1 |
| 10 | Kinetics of cellulose pyrolysis in nitrogen and steam显示文摘 | Antal M J Friedman H L Rogers F E | 1980 | Combustion Science and Technology1980，21，： | 1 |
| 11 | A new method for improved hub height mean wind speed estimates using short-term hub height data显示文摘 | Lackner M A Rogers A L Manwell J F | 2010 | Renewable Energy2010，35，10： | 1 |
| 12 | Plant community,primary productivity,and environmental conditions following wetland reestablishment in the Sacramento-San Joaquin Delta,California显示文摘 | ROBIN L M ROGER F | | 0，，： | 1 |
| 13 | Prevalence and age distribution of human papillomavirus infection in a population of Inuit women in Nunavik, Quebec 显示文摘 | Hamlin-Douglas LK Coutlee F Roger M | 2008 | Cancer Epidemiol Biomarkers Prey2008，17，： | 1 |
| 14 | Formation and cot, lapse of metastable particle packings and open structure in loess deposits显示文摘 | Assallay A M Rogers C D F Smatley I J | 1997 | Engeering Geology1997，48，12： | 1 |
| 15 | Fresh cucumber flavor in refrigerated pickles: comparison of sensory and instrumental analysis显示文摘 | CHERYL P H ROGER E M FEETERS H P F | 2002 | Food Chemistry2002，50，： | 1 |
| 16 | Emphyse ma: e{ect of Reconstruction algorithm on CT imaging measures 显示文摘 | Boedeker K L Mcnitt Gray M F Rogers S R | 2004 | Radiology2004，232，1： | 1 |
| 17 | Engineering properties of lo-ess in Libya 显示文摘 | Assallay A M Rogers C D F Smalley I J | 1996 | Journal of Arid Environments1996，32，4： | 1 |
| 18 | Immobilization and electrochemical redox behavior of cytochrome c on fullerene film-modified electrodes显示文摘 | Souza F D Rogers L M ODell E S | 2005 | Bioelectrochemistry2005，66，： | 1 |
| 19 | Composition ofa ch- emopreventive proanthocyanidin-rich fraction from cranberry fruits responsible for the inhibition of 12-O-tetradecanoyl Pho- rbol-β-aeetate (TPA) induced ornithine decarboxylase (ODC) activity显示文摘 | Kandil F E Smith M A L Rogers R B | 2002 | Journal of Agricultural and Food Chemistry2002，50，5： | 1 |
| 20 | Engineering Properties of Loess in Libya 显示文摘 | ASSALLAY A M ROGERS C D F SMALLEY I J | 1996 | Journal of Arid Environments1996，32，4： | 1 |
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