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| 1 | Differential Proteins Expressed in Rice Leaves and Grains in Response to Salinity and Exogenous Spermidine Treatments显示文摘Exogenous application of spermidine(Spd) has been reported to modulate physiological processes and alleviate salt-induced damage to growth and productivity of several plants including rice. Employing a proteomic approach, we aimed at identifying rice leaf and grain proteins differentially expressing under salt stress, and in response to Spd prior to Na Cl treatment. A total of 9 and 20 differentially expressed protein spots were identified in the leaves of salt-tolerant(Pokkali) and saltsensitive(KDML105) rice cultivars, respectively. Differential proteins common to both cultivars included a photosynthetic light reaction protein(oxygen-evolving complex protein 1), enzymes of Calvin cycle and glycolysis(fructose-bisphosphate aldolase and triose-phosphate isomerase), malate dehydrogenase, superoxide dismutase and a hypothetical protein(Os I_18213). Most proteins were present at higher intensities in Pokkali leaves. The photosynthetic oxygen-evolving enhancer protein 2 was detected only in Pokkali and was up-regulated by salt-stress and further enhanced by Spd treatment. All three spots identified as superoxide dismutase in KDML105 were up-regulated by Na Cl but down-regulated when treated with Spd prior to Na Cl, indicating that Spd acted directly as antioxidants. Important differential stress proteins detected in mature grains of both rice cultivars were late embryogenesis abundant proteins with protective roles and an antioxidant protein, 1-Cys-peroxiredoxin. Higher salt tolerance of Pokkali partly resulted from higher intensities and more responsiveness of the proteins relating to photosynthesis light reactions, energy metabolism, antioxidant enzymes in the leaves, and stress proteins with protective roles in the grains. | Paweena SALEETHONG Sittiruk ROYTRAKUL Kanlaya KONG-NGERN Piyada THEERAKULPISUT | 2016 | Rice science2016，23，1： | 2 |
| 2 | Nevirapine induces apoptosis in liver(HepG2) cells显示文摘Objective: To generate insights into the mechanism of NVP induced hepatotoxicity. Methods: Liver(HepG2) cells were cultured with various concentrations of NVP. This cell line was chosen because it has low expression of cytochrome P450, allowing evaluation of the effects of NVP rather than specific metabolites. Cytotoxicity was determined using a proliferation assay and cell numbers were monitored using trypan blue exclusion assay for long term culture experiments and apoptosis induction was determined by morphological and biochemical investigation. Results: HepG2 cells treated with the highest concentration of NVP tested(819 μM) initially showed a rounded morphology and all cells had died by week three of exposure. Nuclear condensation and fragmentation, increased Annexin V/propidium iodide staining and caspase 9 activation all supported the induction of apoptosis in HepG2 cells in response to NVP treatment. Conclusions: There is a clear induction of apoptosis in response to NVP which suggests that NVP has significant cytotoxicity, over and above any cytotoxicity of metabolites and may contribute directly to patient hepatotoxicity. | Jeerang Wongtrakul Atchara Paemanee Phitchayapak Wintachai Chutima Thepparit Sittiruk Roytrakul Thananya Thongtan Kanokwan Janphen Khuanchai Supparatpinyo Duncan R.Smith | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，6： | 1 |
| 3 | Glycoproteomics analysis of plasma proteins associated with Opisthorchis viverrini infection-induced cholangiocarcinoma in hamster model显示文摘Objective: To apply lectin affinity chromatography and glycoproteomics-based LC-MS/MS to preliminarily investigate the possible potential plasma biomarkers of Opisthorchis viverrini(OV)-associated CCA in OV/dimethylnitrosamine(DMN)-induced CCA hamster model. Methods: Nine Syrian hamsters were divided into 3 groups as follows(n=3 each): normal(healthy control group); OV group; and OV/DMN group(CCA group). Pooled plasma samples collected from animals in each group at the 6th month post-infection with OV metacercarae were subjected to glycoproteomics analysis. Glycoproteins in the pooled sample from each group were initially isolated by concanavilin A(Con A)-based affinity chromatography. The expression of glycoproteins isolated by both enrichment methods were determined using LCMS/MS. Results: Among the 24 Con A-binding glycoproteins isolated, two proteins, N-myc downstream regulated gene 1(NDRG1) and fetuin-B(FETUB) were found up-regulated only in the samples from the OV and control groups, but not in the OV/DMN(CCA) groups. On the other hand, one protein, i.e., NSFL1 cofactor p47 isoform x3(NSFL1C) was found only in the samples from OV/DMN(CCA) and control groups, but not in the OV group. The remaining 21 proteins were upregulated in the samples from all groups. Conclusions: NDRG1, FETUB and NSFL1 C glycoproteins isolated by Con A-based affinity chromatography could be potential biomarkers for CCA. Plasma samples with negative for NDRG1 and FETUB proteins but positive for NSFL1 C are likely to be OV-associated CCA. Nevertheless, this conclusion remains to be confirmed whether this battery test can discriminate OV-associated CCA from other risk factors. | Pramote Sriwanitchrak Atchara Paemanee Sittiruk Roytrakul Vithoon Viyanant Kesara Na-Bangchang | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，12： | 1 |
| 4 | Chikungunya in Southeast Asia:understanding the emergence and finding solutions显示文摘 | Pulmanausahakul R Roytrakul S Auewarakul P | 2011 | Int J Infect Dis2011，15，10： | 1 |
| 5 | Novel serum biomarkers to differentiate cholangioearcinoma from benign biliary tract diseases using a proteomia approach 显示文摘 | JANVILISRI T LEELAWAT K ROYTRAKUL S et o2 | 2015 | Disease Markers2015，2015，10： | 1 |
| 6 | Silver nanoparticles induce toxicity in A549 cellsvia ROS-dependent and ROS-independent pathways显示文摘 | Chairuangkitti P Lawanprasert S Roytrakul S | 2013 | Toxicol In Vitro2013，27，1： | 1 |
| 7 | Comparative proteomics of Cannabis sativa plant tissues 显示文摘 | Raharjo T J Widjaja I Roytrakul S | 2004 | Journal of Biomolecular Techniques2004，15，2： | 1 |
| 8 | Role of vacuolar transporter pro-teins in plant secondary metabolism:Catharanthus roseus cell cul-ture显示文摘 | ROYTRAKUL S VERPOORTE R | 2007 | Phytochemistry Review2007，6，3： | 1 |
| 9 | Cloning of a cDNA encoding 1 - deoxy - xylulo se 5 - pho sphatesynthase from Morinda citrifolia and analysis of its expression in relation to anth raq uinone accumulation 显示文摘 | HAN Y S ROYTRAKUL S VERBERNE M C | 2003 | Plant Science2003，164，6： | 1 |
| 10 | A unique insertion of low complexity amino acid sequence underlies protein-protein interaction in human malaria parasite orotate phosphoribosyltransferase and orotidine 5'-monophosphate decarboxylase显示文摘Objective:To investigate the multienzyine complex formation of human malaria parasite Plasmodium falciparum[P.falciparum)orotate phosphoribosyltransferase(OPRT)and orotidine5'-monophosphate decarboxylase(OMPDC),the fifth and sixth enzyme of the de novo pyrimidine biosynthetic palhway.Previously,we have clearly established that the two enzymes in the malaria parasite exist physically as a heterotetrameric(OPRT)_2(OMPDG)_2 complex containing two subunits each of OPRT and OMPDC.and that the complex have catalytic kinetic advantages over the monofunetional enzyme.Methods:Both enzymes were cloned and expressed as recombinant proteins.The protein-protein interaction in the enzyme complex was identified using bifunctionul chemical cross-linker,liquid chromatography-mass spectrometric analysis and homology modeling,Results:The unique insertions of low complexity region at the a 2 and a 5 helices of the parasite OMPDC,characterized by single amino acid repeat sequence which was not found in homologous proteins from other organisms,was located on the OPRT-OMPDC interface.The structural models for the protein-prolein interaction of the helerotetrameric(OPRT)_2(OMPDC)_2multienzyme complex were proposed.Conclusions:Based on the proteomic data and structural modeling,it is surmised that the human malaria parasite low complexity region is responsible for the OPRT-OMPDC interaction.The structural complex of the parasite enzymes,thus,represents an efficient functional kinetic advantage,which in line with co-localization principles of evolutional origin,and allosteric control in protein-protein-interactions. | Waranya Imprasittichai Sittiruk Roytrakul Sudaratana R.Krungkrai Jcrapan Krungkrai | 2014 | Asian Pacific Journal of Tropical Medicine2014，7，3： | 1 |
| 11 | Determination of the Plasmodium vivax schizont stage proteome显示文摘 | Wanlapa Roobsoong Sittiruk Roytrakul Jetsumon Sattabongkot Jianyong Li Rachanee Udomsangpetch Liwang Cui | 2011 | Journal of Proteomics2011，，9： | 1 |
| 12 | Magnetic bioassembly platforms towards the generation of extracellular vesicles from human salivary gland functional organoids for epithelial repair显示文摘Salivary glands(SG)are exocrine organs with secretory units commonly injured by radiotherapy.Bio-engineered organoids and extracellular vesicles(EV)are currently under investigation as potential strategies for SG repair.Herein,three-dimensional(3D)cultures of SG functional organoids(SGo)and human dental pulp stem cells(hDPSC)were generated by magnetic 3D bioassembly(M3DB)platforms.Fibroblast growth factor 10(FGF10)was used to enrich the SGo in secretory epithelial units.After 11 culture days via M3DB,SGo displayed SG-specific acinar epithelial units with functional properties upon neurostimulation.To consistently develop 3D hDPSC in vitro,3 culture days were sufficient to maintain hDPSC undifferentiated genotype and phenotype for EV generation.EV isolation was performed via sequential centrifugation of the conditioned media of hDPSC and SGo cultures.EV were characterized by nanoparticle tracking analysis,electron microscopy and immunoblotting.EV were in the exosome range for hDPSC(diameter:88.03±15.60 nm)and for SGo(123.15±63.06 nm).Upon ex vivo administration,exosomes derived from SGo significantly stimulated epithelial growth(up to 60%),mitosis,epithelial progenitors and neuronal growth in injured SG;however,such biological effects were less distinctive with the ones derived from hDPSC.Next,these exosome biological effects were investigated by proteomic arrays.Mass spectrometry profiling of SGo exosomes predicted that cellular growth,development and signaling was due to known and undocumented molecular targets downstream of FGF10.Semaphorins were identified as one of the novel targets requiring further investigations.Thus,M3DB platforms can generate exosomes with potential to ameliorate SG epithelial damage. | Ajjima Chansaenroj Christabella Adine Sawanya Charoenlappanit Sittiruk Roytrakul Ladawan Sariya Thanaphum Osathanon Sasitorn Rungarunlert Ganokon Urkasemsin Risa Chaisuparat Supansa Yodmuang Glauco R.Souza Joao N.Ferreira | 2022 | Bioactive Materials2022，7，12： | 1 |
| 13 | Cloning of a cDNA encoding 1-deoxy-D-xylulose 5-phosphate synthase from Morinda citrifolia and analysis of its expression in relation to anthraquinone accumulation显示文摘 | Han YS Roytrakul S Verbeme MC 2003 | 2003 | Plant Sci2003，164，： | 1 |
| 14 | Leaf proteomic analysis in cassava (Manihot esculenta Crantz)during plant development, from planting of stem cutting to storage root formation显示文摘 | Mitprasat M Roytrakul S Jiemsup S | 2011 | Planta2011，233，： | 1 |
| 15 | Proteomics analysis of siRNA -mediated silencing of Wilms'tumor 1 in the MDA - MB -468 breast cancer cell line显示文摘 | Chesor M Roytrakul S Graldist P | 2014 | Oncal Rep2014，31，4： | 1 |
| 16 | Proteomic analysis of salivary glands of female Anopheles barbirostris species A2 (Diptera: Culicidae) by two-dimensional gel electrophoresis and mass spectrometry显示文摘 | Jariyapan N Roytrakul S Paemanee A | 2012 | Parasitol Res2012，111，3： | 1 |
| 17 | Characterization and identification of angiotensin I-converting enzyme (ACE) inhibitory peptides derived from tilapia using Virgibacillus halodenitrificans SK1-3-7 proteinases显示文摘 | TOOPCHAM T ROYTRAKUL S YONGSAWATDIGUL J | 2015 | Journal of Functional Foods2015，14，4： | 1 |
| 18 | Novel fibrinolytic enzymes from Virgibacillus halodenitrificans SK1-3-7 isolated from fish sauce fermentation显示文摘 | Aungkawipa Montriwong Sasitorn Kaewphuak Sureelak Rodtong Sittirak Roytrakul Jirawat Yongsawatdigul | 2012 | Process Biochemistry2012，，12： | 1 |
| 19 | Comparative proteomics of Cannabis sativa plant tissues显示文摘 | Raharjo Tri J Widjaja Ivy Roytrakul Sittiruk et a1 | 2004 | Journal of Biomolecu1ar Techniques2004，15，2： | 1 |
| 20 | Leaf proteomic analysis in aassava ( Manihot esculenta, Crantz ) during plant development, from planting of stem cutting to storage root formation 显示文摘 | Mitprasat M Roytrakul S Jiemsup S | 2011 | Planta2011，233，： | 1 |
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