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| 1 | Synthesis and paste properties of octenyl succinic anhydride modified early Indica rice starch显示文摘Octenyl succinic anhydride (OSA) modified early Indica rice starch was prepared in aqueous slurry systems using response surface methodology. The paste properties of the OSA starch were also investigated. Results indicated that the suitable parameters for the preparation of OSA starch from early Indica rice starch were as follows: reaction period 4 h, reaction tem- perature 33.4 °C, pH of reaction system 8.4, concentration of starch slurry 36.8% (in proportion to water, w/w), amount of OSA 3% (in proportion to starch, w/w). The degree of substitution was 0.0188 and the reaction efficiency was 81.0%. The results of paste properties showed that with increased OSA modification, the starch derivatives had higher paste clarity, decreased retro- gradation and better freeze-thaw stability. | SONG Xiao-yan CHEN Qi-he RUAN Hui HE Guo-qing XU Qiong | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，10： | 51 |
| 2 | Prevalence of Thyroid Nodules and Its Relationship with Iodine Status in Shanghai:a Population-based Study显示文摘Objective This study was designed to evaluate the prevalence of thyroid nodules(TNs) and its relationship with urine iodine concentrations(UICs) after the regional rapid economic growth and lifestyle changes. Methods A cross-sectional survey was conducted in the general population aged 15-69 years. A questionnaire regarding general and personal characteristics and relevant information was administered. Ultrasonography of the thyroid was performed, and serum triiodothyronine(T3), tetraiodothyronine(T4), serum thyroid stimulating hormone(TSH), free triiodothyronine(FT3), free tetraiodothyronine(FT4), thyroglobulin antibody(Tg Ab), thyroid peroxidase antibody(TPOAb), and TSH receptor antibody(TRAb) levels were measured for each individual subject. Results The prevalence rates of TNs in the whole population, females and males were 27.76%, 34.04%, and 21.60%, respectively. The prevalence of multiple nodules increased with age, whereas the prevalence peaks differed between males and females. The median UICs in the whole population and females with non-TNs were higher than those of subjects with TNs(P=0.0035, P=0.0068). The median UICs in subjects with a single TN were higher than those in subjects with multiple TNs(P=0.0164, P=0.0127). The result showed a U-shaped curve relationship between UIC and prevalence of TNs. The prevalence of TNs was the lowest when the UIC was 140-400 μg/L. Conclusion The prevalence of TNs was nearly 30% and increased with age. The relationship between UIC and prevalence of TNs is U-shaped, with an increase in risk when the UIC was <140 μg/L and >400 μg/L. Very low or high UIC levels need attention and correction. | SONG Jun ZOU Shu Rong GUO Chang Yi ZANG Jia Jie ZHU Zhen Ni MI Ming HUANG Cui Hua YU Hui Ting LU Xi RUAN Ye WU Fan | 2016 | Biomedical and Environmental Sciences2016，29，6： | 25 |
| 3 | Pretreatment HIV drug resistance in adults initiating antiretroviral therapy in China,2017显示文摘Background:After the scale-up of antiretroviral therapy(ART)for HIV infected people,increasing numbers of patients have pretreatment drug resistance(PDR).In this study,the prevalence of PDR was evaluated in adults initiating antiretroviral therapy in China.Methods:Blood samples were obtained from 1943 patients who initiated antiretroviral therapy(ART)in 2017 from 13 provinces or cities in China.Pol sequences were used to analyze drug resistance and construct transmission networks.Logistic regression model was used to estimate the potential factors associated with PDR.Results:In total,1711 eligible patients(76.0%male;87.8%aged≥25 years)were included,of which 117(6.8%)had PDR.The highest rates of PDR were 12.2%in Liangshan Prefecture of Sichuan and 9.3 and 8.9%in Dehong and Lincang Prefecture of Yunnan.A multivariate logistic regression analysis revealed that PDR was significantly higher among intravenous drug users(adjusted Odds Ratio(aOR)=2.64,95%CI:1.57–4.44)and individuals from Liangshan,Dehong,and Lincang(aOR=2.04,95%CI:1.26–3.30).In total,754 sequences were used to generate 164 transmission networks.Five transmission networks had two or three sequences containing the same mutations,two networks contained subjects from Liangshan,and one network contained subjects from Dehong.Conclusions:Overall,the PDR prevalence was moderate,with a particularly high prevalence in areas with severe HIV epidemics.These results indicate the importance of continuous PDR monitoring in patients initiating antiretroviral therapy. | Rui-Hua Kang Shu-Jia Liang Yan-Ling Ma Shu Liang Lin Xiao Xin-Hui Zhang Hong-Yan Lu Xiao-Qin Xu Shui-Bin Luo Xiao-Guang Sun Lin Chen Jian-Mei He Guo-Hui Wu Ling-Jie Liao Hui Xing Yi-Ming Shao Yu-Hua Ruan | 2020 | Infectious Diseases of Poverty2020，9，3： | 17 |
| 4 | Changing Proportions of HIV-1 Subtypes and Transmitted Drug Resistance Among Newly Diagnosed HIV/AIDS Individuals—China,2015 and 2018显示文摘Introduction:With the expansion of human immunodeficiency virus(HIV)antiretroviral therapy(ART),HIV drug resistance is becoming more and more serious.This study describes the changing prevalence of HIV-1 subtypes and transmitted drug resistance(TDR)among newly diagnosed individuals in China,2015 and 2018.Methods:A total of 8,980 individuals in 2015 and 2018 from 31 provincial-level administrative divisions(PLADs)were enrolled in this study.Viral RNAs were amplified and sequenced using an in-house polymerase chain reaction(PCR)protocol.The Stanford HIV Drug Resistance Database(HIVdb)was used to predict susceptibility to 12 antiretroviral drugs.Results:The prevalence of TDR was not significantly increased over time.The prevalence of TDR was 3.8%and 4.4%in 2015 and 2018,respectively(P=0.13).The prevalence of CRF55_01B increased from 2.3%in 2015 to 3.9%in 2018(P<0.001).The drug resistance prevalence of nonnucleoside reverse transcriptase inhibitors(NNRTI)increased from 2.4%in 2015 to 3.3%in 2018(P<0.01).The prevalence of E138(P<0.001),H221(P=0.03),and V179(P<0.001)mutations increased from 0.30%,0.09%,and 0.70%in 2015 to 1.10%,0.30%,and 1.70%in 2018,respectively.Conclusions:HIV drug resistance affects the effect of antiretroviral treatment,so the monitoring of HIV TDR should be strengthened to control the transmission of HIV drug resistance. | Jingjing Hao Shan Zheng Mengze Gan Aobo Dong Ruihua Kang Miaomiao Li Shuai Zhao Jing Hu Chang Song Lingjie Liao Yi Feng Yiming Shao Yuhua Ruan Hui Xing | 2021 | China CDC weekly2021，3，53： | 13 |
| 5 | Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders显示文摘孤独性光谱混乱(ASD ) 是有异构的病原学的许多人的神经病学的混乱。E3 ubiquitin (Ub ) 的活动过度 ligase UBE3A,不完全地源自 15q11-q13 拷贝数字变化,为世界范围的 ASD 盒子的 1%-3% 的报道,而是内在的机制遗体描绘了。这里,我们报导 ALDH1A2 的功能, retinoic 酸(RA ) 的限制率的酶合成,被 UBE3A 否定地以一种 ubiquitylation 依赖的方式调整。过多的 UBE3A 剂量被发现损害调停 RA 的 neuronal homeostatic synaptic 粘性。象 ASD 一样症状被 overexpressing UBE3A 在前额的外皮或由一个 ALDH1A 对手的管理在老鼠概括,而 RA 补充显著地减轻了过多的 UBE3A 导致剂量的象 ASD 一样显型。由识别在 ASD 作为内在的机制发信号的减少的 RA,显型连接了到 UBE3A 活动过度,我们的调查结果介绍 ASD 病原学的新远景并且对这逐渐地流行的疾病便于治疗学的开发的一个模式。 | Xingxing Xu Chuanyin Li Xiaobo Gao Kun Xia Hui Guo Yali Li ZijianHao Lei Zhang Daming Gao Chenfan Xu Huatai Xu Zhi-Qi Xiong Zilong Qiu Ling Mei Xiaoduo Xie Kangcheng Ruan Ronggui Hu | 2018 | Cell Research2018，28，1： | 13 |
| 6 | Dyslipidaemia among diabetic patients with ischemic stroke in a Chinese hospital显示文摘背景 Dyslipidaemia 是一个潜在的独立风险因素因为在有这研究的 diabetes.The 目的病人的脑血管的疾病是调查 dyslipidaemia ,在有 ischemic 的糖尿病的病人之中的 dyslipidaemia 的治疗和控制在中国 hospital.Methods 摸 2 个糖尿病的病人被分到糖尿病与的 1046 类型的一个总数( n=522 )并且没有中风 groups.The 的糖尿病二个组被性,年龄和糖尿病匹配 duration.Lipid 和脂蛋白侧面是 measured. | WANG Shao-hua SUN Zi-lin RUAN Xiong-zhong GUO Yi-jing WANG Yao JIN Hui YUAN Yang WEI Qiong | 2009 | Chinese Medical Journal2009，，21： | 11 |
| 7 | Deep learning applied to two-dimensional color Doppler flow imaging ultrasound images significantly improves diagnostic performance in the classification of breast masses:a multicenter study显示文摘Background:The current deep learning diagnosis of breast masses is mainly reflected by the diagnosis of benign and malignant lesions.In China,breast masses are divided into four categories according to the treatment method:inflammatory masses,adenosis,benign tumors,and malignant tumors.These categorizations are important for guiding clinical treatment.In this study,we aimed to develop a convolutional neural network(CNN)for classification of these four breast mass types using ultrasound(US)images.Methods:Taking breast biopsy or pathological examinations as the reference standard,CNNs were used to establish models for the four-way classification of 3623 breast cancer patients from 13 centers.The patients were randomly divided into training and test groups(n=1810 vs.n=1813).Separate models were created for two-dimensional(2D)images only,2D and color Doppler flow imaging(2D-CDFI),and 2D-CDFI and pulsed wave Doppler(2D-CDFI-PW)images.The performance of these three models was compared using sensitivity,specificity,area under receiver operating characteristic curve(AUC),positive(PPV)and negative predictive values(NPV),positive(LR+)and negative likelihood ratios(LR-),and the performance of the 2D model was further compared between masses of different sizes with above statistical indicators,between images from different hospitals with AUC,and with the performance of 37 radiologists.Results:The accuracies of the 2D,2D-CDFI,and 2D-CDFI-PW models on the test set were 87.9%,89.2%,and 88.7%,respectively.The AUCs for classification of benign tumors,malignant tumors,inflammatory masses,and adenosis were 0.90,0.91,0.90,and 0.89,respectively(95%confidence intervals[CIs],0.87-0.91,0.89-0.92,0.87-0.91,and 0.86-0.90).The 2D-CDFI model showed better accuracy(89.2%)on the test set than the 2D(87.9%)and 2D-CDFI-PW(88.7%)models.The 2D model showed accuracy of 81.7%on breast masses≤1 cm and 82.3%on breast masses>1 cm;there was a significant difference between the two groups(P<0.001).The accuracy of the CNN classifications for the test set(89.2%)was significantly higher than that of all the radiologists(30%).Conclusions:The CNN may have high accuracy for classification of US images of breast masses and perform significantly better than human radiologists.Trial registration:Chictr.org,ChiCTR1900021375;http://gffzz62a03ee25e6f4a77hfpncnx5c0wfk6c5v.ffgz.tsg.suse.edu.cn/showproj.aspx?proj=33139. | Teng-Fei Yu Wen He Cong-Gui Gan Ming-Chang Zhao Qiang Zhu Wei Zhang Hui Wang Yu-Kun Luo Fang Nie Li-Jun Yuan Yong Wang Yan-Li Guo Jian-Jun Yuan Li-Tao Ruan Yi-Cheng Wang Rui-Fang Zhang Hong-Xia Zhang Bin Ning Hai-Man Song Shuai Zheng Yi Li Yang Guang | 2021 | Chinese Medical Journal2021，，4： | 11 |
| 8 | Epidemiology,clinical and laboratory characteristics of currently alive HIV-1 infected former blood donors naive to antiretroviral therapy in Anhui Province,China显示文摘Background Unregulated commercial blood/plasma collection among farmers occurred between 1992 and 1995 in central China and caused the second major epidemic of human immunodeficiency virus type 1 (HIV-1) infection in China. It is important to characterize HIV-1-infected former blood donors and to study characteristics associated with disease progression for future clinical intervention and vaccine development. Methods A cross-sectional study was performed on HIV-1-infected former blood donors (FBDs) and age-matched HIV-seronegative local residents. Demographic, epidemiologic, clinical and key laboratory data were collected from all study participants. Both unadjusted and adjusted multivariate linear regressions were employed to analyze the association of the decrease of CD4+ T-cell counts with other characteristics. Results Two hundred and ninety-four HIV-1-infected FBDs and 59 age-matched HIV-seronegative local residents were enrolled in this study. The unregulated blood/plasma collection occurred more than a decade (10.8 -12.8 years) ago, which caused the rapid spread of HIV-1 infection and the high prevalence of co-infection with hepatitis C virus (HCV, 89.5%); hepatitis B virus (HBV) co-infection was observed in only 11 HIV+participants (3.7%). Deterioration in both clinical manifestation and laboratory parameters and increase of viral loads were observed in parallel with the decrease of CD4+ T-cell counts. The decrease of total lymphocyte counts (P<0.001) and hemoglobin levels (P<0.001) and the appearance of dermatosis (P=0.03) were observed in parallel with the decrease of CD4+ T-cell counts whereas viral loads (P<0.001) and CD8+ T-cell counts (P=0.01) were inverselyassociated with CD4+ T-cell counts. Conclusions Co-infection with HCV but not HBV is highly prevalent among HIV-1-infected FBDs. CD4+ T-cell counts is a reliable indicator for disease progression among FBDs. Total lymphocyte counts, hemoglobin level and appearance of dermatosis were positively associated with CD8+ T-cell counts and viral loads were inversely associated with the decreased CD4+ T-cell counts. | XU Jian-qing WANG Jian-jun HAN Li-feng XU Chen RUAN Yu-hua XU Zhen-hou CHEN Xi LIU Zhen-dong WANG Jun SU Bing DING Xin-ping GAO Bing GU Yong-bin CAO Xiao-yun XING Hui HONG Kun-xue PENG Hong ZHAO Quan-bi YUAN Lin FENG Yi ZHANG Gui-yun MA Li-ying WU Lan SHAO Yi-ming | 2006 | Chinese Medical Journal2006，，23： | 9 |
| 9 | Production of conjugated linoleic acids by Lactobacillus plantarum strains isolated from naturally fermented Chinese pickles显示文摘Naturally fermented pickles harbour many lactic acid bacteria(LAB).Forty-three LAB strains with conjugated linoleic acid(CLA)-producing ability were isolated from three naturally fermented pickle brines.Of these isolates,lp15 identified as Lactobacillus plantarum by API 50 CHL system and full-length 16S rDNA sequence analysis exhibited the highest CLA-producing ability(26.1% conversion) at 48 h in de Man Rogosa Sharpe(MRS) broth in the presence of 100 μg/ml of linoleic acid(LA).Compared to other strains,L.plantarum strain lp15 showed the highest tolerance upon increased levels of LA in the medium,i.e.,up to 600 μg/ml.This strain converted about 25% of LA into CLA isomers [predominantly cis-9,trans-11 CLA(9-CLA) and trans-10,cis-12 CLA(10-CLA)],of which 75% was 9-CLA.Interestingly,though the conversion rate of LA into CLA by lp15 remained stable between 100 to 600 μg/ml LA levels in the medium,it dropped sharply at 1000 μg/ml.Taken together,the lp15 strain displayed relatively high LA tolerance with higher conversion rate,which implies that this strain is a valuable candidate for enhancing the CLA content in food-sources like pickles. | Pei LIU Sheng-rong SHEN Hui RUAN Qian ZHOU Liu-liu MA Guo-qing HE | 2011 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2011，12，11： | 8 |
| 10 | R5 to X4 coreceptor switch of human immunodeficiency virus type 1B' and B'/C recombinant subtype isolates in China显示文摘The chemokine receptors CCR5 and CXCR4 play animportant role as coreceptors for humanimmunodeficiency virus type 1(HIV-1)entring into cells.HIV-1 isolates can be distinguished by the chemokinecoreceptors.Nonsyncytium inducing(NSI),macrophagetropic viruses utilizing CCR5,are called R5 viruses;syncytium inducing(SI)isolates use CXCR4 and knownas X4 viruses.R5 viruses generally are associated withlatent stage of infection and X4 viruses with later。 | GUO Yan-fang MA Li-ying YUAN Lin WANG Shu-hua SUN Jian-ping XU Wei-si Xu Jian-qing XING Hui HONG Kun-xue ZHANG Xiao-yan RUAN Yu-hua ZHANG Yao-xin SHAO Yi-ming | 2007 | Chinese Medical Journal2007，，6： | 7 |
| 11 | Addictive evaluation of cholic acid-verticinone ester, a potential cough therapeutic agent with agonist action of opioid receptor显示文摘 | Jiu-liang ZHANG Hui WANG Chang CHEN Hui-fang PI Han-li RUAN Peng ZHANG Ji-zhou WU | 2009 | Acta Pharmacologica Sinica2009，30，5： | 7 |
| 12 | Implications of biomarkers in human hepatocellular carcinoma pathogenesis and therapy显示文摘Hepatocellular carcinoma(HCC)is one of the most frequent tumors worldwide and accounts for approximately one-third of all malignancies.In the past decade,advances have been made to improve the prognosis of HCC,including improvement in the clinical diagnosis of early-stage HCC using molecular biomarkers and molecular-targeted therapy to treat advanced HCC.However,the diagnosis,pathogenesis and targeted therapy of HCC are not completely independent,and should be comprehensively studied.For example,a number of tumor markers provide useful clinical information not only for prognosis,but also in pathogenesis and treatment efficacy.Therefore,this review will focus on the role of several specific biomarkers implicated in the pathogenesis of HCC and several promising molecular-targeted drugs that target the biomarkers of HCC. | Li-Li Han Yi Lv Hui Guo Zhi-Ping Ruan Ke-Jun Nan | 2014 | World Journal of Gastroenterology2014，20，30： | 7 |
| 13 | Optimization of biotransformation from phytosterol to androstenedione by a mutant Mycobacterium neoaurum ZJUVN-08显示文摘Biotransformation of phytosterol(PS) by a newly isolated mutant Mycobacterium neoaurum ZJUVN-08 to produce androstenedione has been investigated in this paper.The parameters of the biotransformation process were optimized using fractional factorial design and response surface methodology.Androstenedione was the sole product in the fermentation broth catalyzed by the mutant M.neoaurum ZJUVN-08 strain.Results showed that molar ratio of hydroxypropyl-β-cyclodextrin(HP-β-CD) to PS and substrate concentrations were the two most significant factors affecting androstenedione production.By analyzing the statistical model of three-dimensional surface plot,the optimal process conditions were observed at 0.1 g/L inducer,pH 7.0,molar ratio of HP-β-CD to PS 1.92:1,8.98 g/L PS,and at 120 h of incubation time.Under these conditions,the maximum androstenedione yield was 5.96 g/L and nearly the same with the non-optimized(5.99 g/L),while the maximum PS conversion rate was 94.69% which increased by 10.66% compared with the non-optimized(84.03%).The predicted optimum conditions from the mathematical model were in agreement with the verification experimental results.It is considered that response surface methodology was a powerful and efficient method to optimize the parameters of PS biotransformation process. | Xiao-yan ZHANG Yong PENG Zhong-rui SU Qi-he CHEN Hui RUAN Guo-qing HE | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，2： | 7 |
| 14 | 传统酸面团制备的馒头特征风味物质的研究(英文)显示文摘目的:通过同时蒸馏萃取(SDE)、顶空固相微萃取(SPME)及吹扫捕集(P&T)技术结合气相色谱-嗅闻-质谱(GC-O-MS)技术对传统酸面团制备的馒头特征风味成分进行分析,并初步探讨特征风味形成机理。创新点:首次采用不同风味物质分析技术探索传统酸面团制备的馒头关键风味成分。方法:采用同时SDE、SPME及P&T方法,对不同传统酸面团制备的馒头特征风味物质进行分析。结论:SDE结果显示,反式-2-壬烯醛和反式-2,4-癸二烯醛为传统酸面团馒头中关键风味成分。SPME和P&T方法表明乙醇和醋酸是关键风味物质。因此,需要结合不同技术方法,全面分析传统酸面团制备的馒头中特征风味成分。 | Guo-hua ZHANG Tao WU Faizan A.SADIQ Huan-yi YANG Tong-jie LIU Hui RUAN Guo-qing HE | 2016 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2016，17，10： | 7 |
| 15 | Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma显示文摘Fermentation of Phaffia rhodozyma is a major method for producing astaxanthin, an important pigment with industrial and pharmaceutical application. To improve astaxanthin productivity, single factor and mixture design experiments were used to investigate the effects of nitrogen source on Phaffia rhodozyma cultivation and astaxanthin production. Results of single factor experiments showed nitrogen source could significantly affect P. rhodozyma cultivation with respect to carbon source utilization, yeast growth and astaxanthin accumulation. Further studies of mixture design experiments using (NH4)2SO4, KNO3 and beef extract as nitrogen sources indicated that the proportion of three nitrogen sources was very important to astaxanthin production. Validation experiments showed that the optimal nitrogen source was composed of 0.28 g/L (NH4)2SO4, 0.49 g/L KNO3 and 1.19 g/L beef extract. The kinetic characteristics of batch cultivation were investigated in a 5-L pH-stat fermentor. The maximum amount of biomass and highest astaxanthin yield in terms of volume and in terms of biomass were 7.71 mg/L and 1.00 mg/g, respectively. | NI Hui CHEN Qi-he RUAN Hui YANG Yuan-fan LI Li-jun WU Guang-bin Hid Yang HE Guo-qing | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，5： | 6 |
| 16 | Dynamic High Pressure Micro-fluidization Effects on Structure and Physico-chemical Properties of Egg White Protein显示文摘The authors dealt with the effects of dynamic high pressure micro-fluidization(untreated and 20―160 MPa treated) on the structure and physico-chemical properties of egg white solutions[6%(mass fraction) or 61.2 mg protein/mL]. Micro-fluidization treatment resulted in a decrease in mean particle size,and little change in solubility. It was found that the residual denaturation enthalpy was decreased with the increase of pressure from 20 to 100 MPa and from 120 to 160 MPa,reaching into the maximum at 160 MPa. There was an increase of surface hydrophobicity with the maximum at 100 MPa observed. Simultaneously,micro-fluidization treatment also resulted in a decrease in the content of sulfhydryl groups. | TU Zong-cai WANG Hui LIU Cheng-mei LIU Guang-xian CHEN Gang Roger Ruan | 2009 | Chemical Research in Chinese Universities2009，25，3： | 5 |
| 17 | Argininosuccinate synthase 1 suppresses cancer cell invasion by inhibiting STAT3 pathway in hepatocellular carcinoma显示文摘Metastasis is the main reason for high recurrence and poor survival of hepatocellular carcinoma(HCC).The molecular mechanism underlying HCC metastasis remains unclear.In this study,we found that argininosuccinate synthase 1(ASS1)expression was significantly decreased and down-regulation of ASS1 was closely correlated with poor prognosis in HCC patients.DNA methylation led to the down-regulation of ASS1 in HCC.Stable silencing of ASS1 promoted migration and invasion of HCC cells,whereas overexpression of ASS1-inhibited metastasis of HCC cells in vivo and in vitro.We also revealed that ASS1-knockdown increased the phosphorylation level of S727STAT3,which contributed to HCC metastasis by up-regulation of inhibitor of differentiation 1(ID1).These findings indicate that ASS1 inhibits HCC metastasis and may serve as a target for HCC diagnosis and treatment. | Xuemei Tao Qiaozhu Zuo Haoyu Ruan Hui Wang Haojie Jin Zhuoan Cheng Yuanyuan Lv Wenxin Qin Cun Wang | 2019 | Acta Biochimica et Biophysica Sinica2019，51，3： | 4 |
| 18 | Benefits of Early and Immediate Initiation of Antiretroviral Therapy among HIV Patients in Chongqing, China显示文摘In an effort to end the HIV epidemic by 2030, the Joint United Nations Program on HIV/AIDS(UNAIDS)set the ‘90-90-90’ target and aimed to expand the timely use of ART worldwide. By the end of 2017,21.7 million people living with HIV, amounting to59% of HIV patients worldwide, were receiving antiretroviral therapy(ART)[1]. In China, the National Free Antiretroviral Treatment Program(NFATP),which was initiated in 2002, continued to progress. | ZHOU Chao ZHANG Wei LU Rong Rong OUYANG Lin XING Hui SHAO Yi Ming WU Guo Hui RUAN Yu Hua | 2020 | Biomedical and Environmental Sciences2020，33，4： | 4 |
| 19 | A new alkaloid from Lycoris aurea显示文摘新碱从 Lycoris 的灯泡被孤立脲(L’ Herit ) 植物。它的结构包括 2D NMR 通过化学、分光镜的研究作为 3-o-ethyltazettinol 被建立。 | Hui Fang Pi Peng Zhang Han Li Ruan Yong Hui Zhang Han Dong Sun Ji Zhou Wu | 2009 | Chinese Chemical Letters2009，20，11： | 4 |
| 20 | A food-grade industrial arming yeast expressing β-1,3-1,4-glucanase with enhanced thermal stability显示文摘The aim of this work was to construct a novel food-grade industrial arming yeast displaying β-1,3-1,4-glucanase and to evaluate the thermal stability of the glucanase for practical application. For this purpose, a bi-directional vector containing galactokinase (GAL1) and phosphoglycerate kinase 1 (PGK1) promoters in different orientations was constructed. The β-1,3-1,4-glucanase gene from Bacillus subtilis was fused to α-agglutinin and expressed under the control of the GAL1 promoter. α-galactosidase induced by the constitutive PGK1 promoter was used as a food-grade selection marker. The feasibility of the α-galactosidase marker was confirmed by the growth of transformants harboring the constructed vector on a medium containing melibiose as a sole carbon source, and by the clear halo around the transformants in Congo-red plates owing to the expression of β-1,3-1,4-glucanase. The analysis of β-1,3-1,4-glucanase activity in cell pellets and in the supernatant of the recombinant yeast strain revealed that β-1,3-1,4-glucanase was successfully displayed on the cell surface of the yeast. The displayed β-1,3-1,4-glucanase activity in the recombinant yeast cells increased immediately after the addition of galactose and reached 45.1 U/ml after 32-h induction. The thermal stability of β-1,3-1,4-glucanase displayed in the recombinant yeast cells was enhanced compared with the free enzyme. These results suggest that the constructed food-grade yeast has the potential to improve the brewing properties of beer. | Qin GUOt Wei ZHANG Liu-liu MA Qi-he CHEN Ji-cheng CHEN Hong-bo ZHANG Hui RUAN Guo-qing HE | 2010 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2010，11，1： | 4 |
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