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| 1 | Subtyping Animal Influenza Virus with General Multiplex RT-PCR and Liquichip High Throughput (GMPLex)显示文摘This study developed a multiplex RT-PCR integrated with luminex technology to rapidly subtype simultaneously multiple influenza viruses. Primers and probes were designed to amplify NS and M genes of influenza A viruses HA gene of H1, H3, H5, H7, H9 subtypes, and NA gene of the N1 and N2 subtypes. Universal super primers were introduced to establish a multiplex RT-PCR (GM RT-PCR). It included three stages of RT-PCR amplification, and then the RT-PCR products were further tested by LiquiChip probe, combined to give an influenza virus (IV) rapid high throughput subtyping test, designated as GMPLex. The IV GMPLex rapid high throughput subtyping test presents the following features: high throughput, able to determine the subtypes of 9 target genes in H1, H3, H5, H7, H9, N1, and N2 subtypes of the influenza A virus at one time; rapid, completing the influenza subtyping within 6 hours; high specificity, ensured the specificity of the different subtypes by using two nested degenerate primers and one probe, no cross reaction occurring between the subtypes, no non-specific reactions with other pathogens and high sensitivity. When used separately to detect the product of single GM RT-PCR for single H5 or N1 gene, the GMPLex test showed a sensitivity of 10-5(= 280ELD50) forboth tests and the Luminex qualitative ratio results were 3.08 and 3.12, respectively. When used to detect the product of GM RT-PCR for H5N1 strain at the same time, both showed a sensitivity of 10-4(=2800 ELD50). The GMPLex rapid high throughput subtyping test can satisfy the needs of influenza rapid testing. | Zhi-feng Qin Jie Sun Ti-kang Lu Shao-ling Zeng Qun-yi Hua Qing-yan Ling Shu-kun Chen Jian-qiang Lv Cai-hong Zhang Bing Cheng Zhou-xi Ruan Ying-zuo Bi Joseph J Giambrone Hong-zhuan Wu | 2012 | Virologica Sinica2012，27，2： | 8 |
| 2 | Gastric cancer in young people under 30 years of age: worse prognosis, or delay in diagnosis?显示文摘 | Horacio N López-Basave Diaz Romero C Alejandro Padilla Rosciano Melchor Ruan J Silvio A. ?amendys-Silva Vela Sarmiento I Ruiz Molina JM Calderillo Ruiz G Flavia Morales-Vásquez Abelardo Meneses Garcia Angel Herrera-Gomez | 2013 | Cancer Management and Research (default)2013，，： | 2 |
| 3 | Thermal decomposition of bauxite minerals: infrared emission spectroscopy of gibbsite显示文摘 | Kloprogge J T Ruan H D Frost R L | 2004 | Boehmite and Diaspore2004，37，6： | 1 |
| 4 | The behavior of hydroxyl units of synthetic goethite and its dehydroxylated product hematite显示文摘 | Ruan H D Frost R L Kloprogge J T | 2001 | Spectrochim Acta Part A2001，57，： | 1 |
| 5 | Actin, a reliable marker of internal control 显示文摘 | RUAN W J LAI M D | 2007 | Climica Chimica Acta2007，385，12： | 1 |
| 6 | A comparative study of nanosilica/poly(propylene) composites prepared by reactive compatibilization 显示文摘 | Chen J H Rong M Z Ruan W H Mai Y L Zhang M Q | 2008 | Macromolecular Chemistry and Physics2008，209，： | 1 |
| 7 | Towards Arabidopsis genome analysisz monitoring expression profiles of 1400 genes using cDNA microarrays显示文摘 | Ruan Y J Gilmore J Conner T | 1998 | The Plant Journal1998，，6： | 1 |
| 8 | Solid - Phase Grafting of Glycidyl Methacrylate onto Polypropylane显示文摘 | Y K PAN J M RUAN D F ZHAN | 1996 | J Appl Polym Sci1996，65，： | 1 |
| 9 | Decay of correlations implies chaos in the sense of Devaney显示文摘 | Xu Z J Lin W Ruan J | 2004 | Chaos Solitons and Fractals2004，22，： | 1 |
| 10 | Lack of effects of vitamin E on aluminum-induced deficit of synaptic plasticity in rat dentate gyrus in vivo显示文摘 | Wang M Ruan D Y Chen J T | 2002 | Food Chem Toxicol2002，40，： | 1 |
| 11 | Global dynamic analysis of general Co- hen-Grossberg neural networks with impulse 显示文摘 | Chen Z Ruan J | 2007 | Chaos Solitons and Fractals2007，32，： | 1 |
| 12 | A nonlinear control for enhancing HVDC light transmission system stability显示文摘 | Ruan S Y Li G J Peng L | 2007 | International Journal of Electrical Power and Energy Systems2007，29，7： | 1 |
| 13 | A new approach of direct digital control for spool valves显示文摘 | RUAN J BURTON R | 2002 | ASME The Fluid Power and Systems Technology Division2002，9，6： | 1 |
| 14 | Lycorine:A Potential Broad-Spectrum Agent against Crop Pathogenic Fungi显示文摘 | SHEN J W RUAN Y REN W | 2014 | Journal of Microbiology and Biotechnology2014，324，3： | 1 |
| 15 | HuMiTar:a sequence-based method for prediction of human microRNA targets显示文摘 | Ruan J Chen H Kurgan L | | 0，，： | 1 |
| 16 | Customized Products:A Competitive Analysis显示文摘 | Syam N B Ruan R Hess J D | 2005 | Marketing Science2005，24，4： | 1 |
| 17 | Efficient Generation of Monoclonal Antibodies Against Major Structural Proteins of Rabies Virus with Suckling Mouse Brain Antigen显示文摘 | Zhang J Ruan X Zan J | 2014 | Monoclonal antibodies in immunodiagnosis and immunotherapy2014，33，2： | 1 |
| 18 | Inducible and cardiac specific PTEN inactivation protects ischemia/reperfusion injury显示文摘 | Ruan H Li J Ren S | 2009 | J Mol Cell Cardiol2009，46，2： | 1 |
| 19 | Metabolic profile of Diane-35 versus Diane 35 plus mefformin in Chinese PCOS women under standardized life style changes显示文摘 | Wu H Ruan X Jin J | 2015 | Gynecol Endocrinol2015，31，： | 1 |
| 20 | Self-tuning of fuzzy belief rule bases for engineering system safety analysis显示文摘 | Liu J Yang J B Ruan D | 2008 | Annals Operational Research2008，163，： | 1 |
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