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| 1 | Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system显示文摘允许的技术内长的基因的特定的规定为基因功能的学习是珍贵的并且在治疗学有大潜力。我们创造了在 CRISPR 上系统,由核酸酶死者 Cas9 (dCas9 ) 蛋白质组成的二部件的 transcriptional 使活跃之物与互补顺序与 transcriptional 激活域和单个指南 RNA (sgRNAs ) 熔化了到基因倡导者。我们证明在 CRISPR 上能高效地以一种悦耳的方式在人和老鼠房间激活外长的记者基因。另外,我们证明在 vivo 的柔韧的记者基因激活能被把系统部件注入老鼠接合子完成。而且,我们证明在 CRISPR 上能激活内长的 IL1RN, SOX2,和 OCT4 基因。最有效的基因激活被对近似倡导者有约束力的 3-4 sgRNAs 的簇完成,建议他们在基因正式就职的 synergistic 行动。显著地,当指向多重基因的 sgRNAs 同时被介绍进房间时,柔韧的多路的内长的基因激活被完成。染色体宽的表示介绍表明了系统的高特性。 | Albert W Cheng Haoyi Wang Hui Yang Linyu Shi Yarden Katz Thorold W Theunissen Sudharshan Rangarajan Chikdu S Shivalila Daniel B Dadon Rudolf Jaenisch | 2013 | Cell Research2013，23，10： | 65 |
| 2 | 同步荧光法检测鱼胆汁中的1-羟基芘显示文摘建立了用同步荧光法测定鱼胆汁中的 1 羟基芘 (1 HP)的方法。该方法通过直接测定鱼胆汁的多环芳烃 (PAHs)的代谢产物之一 1 HP ,用于判断PAHs生物有效部分对海岸带养殖水环境的污染程度。以真鲷(Pargrosomusmajor,简称Pm) , 鱼 (Nibeamiichthys ,简称Nm)为实验对象 。 | 张勇 王淑红 朱亚先 Michael H W Lam Rudolf S S Wu 洪华生 | 2002 | 分析化学2002，30，4： | 6 |
| 3 | Late biliary complications in human alveolar echinococcosis are associated with high mortality显示文摘AIM:To evaluate the incidence of late biliary complications in non-resectable alveolar echinococcosis(AE)under long-term chemotherapy with benzimidazoles.METHODS:Retrospective analysis of AE patients with biliary complications occurring more than three years after the diagnosis of AE.We compared characteristics of patients with and without biliary complications,analyzed potential risk factor for biliary complications and performed survival analyses.RESULTS:Ninety four of 148 patients with AE in Zurich had non-resectable AE requiring long-term benzimidazole chemotherapy,of which 26(28%)patients developed late biliary complications.These patients had a median age of 55.5(35.5-65)years at diagnosis of AE and developed biliary complications after 15(8.25-19)years of chemotherapy.The most common biliary complications during long-term chemotherapy were late-onset cholangitis(n=14),sclerosing cholangitis-like lesions(n=8),hepatolithiasis(n=5),affection of the common bile duct(n=7)and secondary biliary cirrhosis(n=7).Thirteen of the 26 patients had undergone surgery(including 12 resections)before chemotherapy.Previous surgery was a risk factor for late biliary complications in linear regression analysis(P=0.012).CONCLUSION:Late biliary complications can be observed in nearly one third of patients with non-resectable AE,with previous surgery being a potential risk factor.After the occurrence of late biliary complications,the median survival is only 3 years,suggesting that late biliary complications indicate a poor prognostic outcome. | Pascal Frei Benjamin Misselwitz Meher K Prakash Alain M Schoepfer Bettina M Prinz Vavricka Beat Müllhaupt Michael Fried Kuno Lehmann Rudolf W Ammann Stephan R Vavricka | 2014 | World Journal of Gastroenterology2014，20，19： | 5 |
| 4 | Basal gang haematomas in noncomatose patients:subacute stereotactic aspiration improves longterm outcome in comparison to purely medical teatment 显示文摘 | Gerhard M Robert W Rudolf WC | 2005 | Neurosurg Rev2005，28，： | 1 |
| 5 | Hydrogenolytic Degradation of Thermosets 显示文摘 | Braun D Gentzkow W Rudolf A P | 2001 | Polym Degrad Stab2001，74，1： | 1 |
| 6 | A novel hybrid of carbon nanotubes/ iron nanopartieles: iron-filled nodule-containing carbon nanotubes显示文摘 | LIU S W RUDOLF J W | 2005 | Carbon2005，43，： | 1 |
| 7 | Nephron-sparing surgery of renal cell carcinoma with a normal opposite kidney: long-term outcome in 180 patients显示文摘 | Dragana Filipas Jan Fichtner Claudia Spix Peter Black Wolfgang Carus Rudolf Hohenfellner Joachim W Thüroff | 2000 | Urology2000，，3： | 1 |
| 8 | Cultured gill epithelial cells from tilapia(Oreochromis niloticus): a new in vitro assay for toxicants显示文摘 | Zhou B S Liu W H Rudolf S S | 2005 | Aquatic Toxicology2005，71，1： | 1 |
| 9 | Spatially disaggregated emission inventory for anthropogenic NMVOC in Austria显示文摘 | WOLFGANG L RUDOLF O WILFRIED W | 1993 | Atmospheric Environment1993，27，16： | 1 |
| 10 | Deposition of composite titanic vanadia thin films by chemical bath deposition显示文摘 | Rudolf C H lars P H J Stefanie W | 2004 | Chem Vialer2004，16，22： | 1 |
| 11 | The first highly potent and selective non-peptide neuropeptide Y Y1 receptor antagonist:BIBP3226显示文摘 | Eberlein W Engel W | 1994 | Eur J Pharmacol1994，271，： | 1 |
| 12 | Revision rate after short-stem total hip arthroplasty显示文摘 | Jakob van Oldenrijk Jeroen Molleman Michel Klaver Rudolf W Poolman Daniel Haverkamp | 2014 | Acta Orthopaedica2014，，3： | 1 |
| 13 | Structural alterations to the podocyte are related to proteinuria in 2 diabetic patients显示文摘 | Kathryn E White W Rudolf W | 2004 | Nephrol Dial Transplant2004，19，6： | 1 |
| 14 | consensus statement: childhood obesity 显示文摘 | SPEISER P W RUDOLF M C J ANHALT H | 2005 | J Clin Endocrinol Metab2005，90，3： | 1 |
| 15 | Fast events in protein folding: relaxation dynamics of secondary and tertiary structure in native apomyoglobin显示文摘 | Rudolf G Skip W Robert H C | 1997 | Proceedings of Natl Acad Sci USA1997，94，： | 1 |
| 16 | Hydrogenolytic degradation of thermosets显示文摘 | BRAUN D GENTZKOW W V RUDOLF AP | 2001 | Polymer Degradation and Stability2001，74，1： | 1 |
| 17 | Deposition of composite titania/vanadia thin films by chemical bath deposition显示文摘 | Lars P H J Stefanie W | 2004 | Chem Mater2004，16，22： | 1 |
| 18 | Post-traumatic syndrome after minor head injury cannot be predicted by neurological investigations显示文摘 | RUDOLF K JOCHEN S JURGEN W | 2004 | Brain & Development2004，26，： | 1 |
| 19 | Phousphates in sewage and sludge treatment: effects on sludge digestion显示文摘 | RUDOLFS W STAHL G W | 1947 | Sewage Works Journal1947，19，3： | 1 |
| 20 | Hydrogenolytic degradation of thermosets 显示文摘 | Braun D Gentzkowl W von Rudolf AP | 2001 | Polymer Degradation and Stability2001，74，： | 1 |
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