|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Engineering nanoparticles for targeting rheumatoid arthritis: Past, present, and future trends显示文摘风湿性关节炎(RA ) 是 synovial 关节发炎和软骨和骨头织物破坏描绘的长期的煽动性的疾病。尽管在那里存在为 RA 的一些处理策略,他们不完全安全、有效。因此,为明确地,目标 inflamed/swollen 连接的 RA 开发并且测试并且同时稀释另外的可能的损坏到健康纸巾新药是重要的。当为对待 RA 想象精确的药时,纳米技术能是一种好选择考虑。通过 nanoparticles 的使用,增加治疗学的 bioavailability 和 bioactivity 并且启用到损坏关节的选择指向是可能的。此处,用为 RA 的处理的 nanoparticles 的最近的研究,也就是与 liposomes,聚合 nanoparticles, dendrimers,和金属性的 nanoparticles,被考察了。这些治疗学的策略在由传统的药在那上改进处理显示出大诺言。这些研究的结果证实 nanoparticles 的使用的可行性主要由于他们的 biocompatibility,低毒性,控制版本,和选择的药交货到在动物 RA 模型的煽动的纸巾。因此,宣称在不久的将来,纳米技术将为象 RA 那样的人的疾病在先进治疗和病人特定的治疗起一个关键作用是可能的。 | Isabel Matos Oliveira Cristiana Goncalves Rui Lufs Reis Joaquim Miguel Oliveira | 2018 | Nano Research2018，11，9： | 11 |
| 3 | Parenteral fish oil as a pharmacological agent to modulate post-operative immune response: A randomized, double-blind, and controlled clinical trial in patients with gastrointestinal cancer显示文摘 | Raquel Susana Matos de Miranda Torrinhas Raquel Santana Thaís Garcia Maria Fernanda Cury-Boaventura Maria Mirtes Sales Rui Curi Dan Linetzky Waitzberg | 2013 | Clinical Nutrition2013，，4： | 3 |
| 4 | Co-expression of monocarboxylate transporter 1 (MCT1) and its chaperone (CD147) is associated with low survival in patients with gastrointestinal stromal tumors (GISTs)显示文摘 | Ant?nio Talvane Torres Oliveira Céline Pinheiro Adhemar Longatto-Filho Maria Jose Brito Olga Martinho Delcio Matos André Lopes Carvalho Vinícius Lima Vazquez Thiago Buosi Silva Cristovam Scapulatempo Sarhan Sydney Saad Rui Manuel Reis Fátima Baltazar | 2012 | Journal of Bioenergetics and Biomembranes2012，，1： | 1 |
| 5 | Co-expression of monocarboxylate transporter 1 (MCT1) and its chaperone (CD147) is associated with low survival in patients with gastrointestinal stromal tumors (GISTs)显示文摘 | Ant?nio Talvane Torres Oliveira Céline Pinheiro Adhemar Longatto-Filho Maria Jose Brito Olga Martinho Delcio Matos André Lopes Carvalho Vinícius Lima Vazquez Thiago Buosi Silva Cristovam Scapulatempo Sarhan Sydney Saad Rui Manuel Reis Fátima Baltazar | 2012 | Journal of Bioenergetics and Biomembranes2012，，1： | 1 |
| 6 | A comparison of the fatigue behavior between S355 and S690 steel grades显示文摘 | Abílio M P Jesus de Rui Matos | 2012 | Journal of Constructional Steel Research2012，79，： | 1 |
| 7 | G-308A TNF-α polymorphism is associated with an increased risk of invasive cervical cancer显示文摘 | Isabel Duarte Alexandra Santos Hugo Sousa Raquel Catarino Daniela Pinto Ana Matos Deolinda Pereira José Moutinho Paulo Canedo José Carlos Machado Rui Medeiros | 2005 | Biochemical and Biophysical Research Communications2005，，2： | 1 |
| 8 | Robotic4all project:Results of a hands-on robotic surgery training program显示文摘Objective Although robotic surgery adoption and its indications are growing worldwide,for multiple factors,including costs,there is a lack of training and experience.Our aim was to study the impact of a robotic introduction training program on gesture performance,such as suturing,in robot-naive individuals.Methods Using the DaVinci robot,a 2-hour program was based on virtual reality and anatomical model exercises.All participants performed 3 repetitions of virtual reality exercises on the virtual simulator,and then performed and were assessed on 2 tests,ie robot and laparoscopic training box.After the course,the participants were surveyed for this training program.Results Twenty-seven residents and surgeons were enrolled in the training program.With only 2 hours of training,all of the participants were able to complete the training program,thus learning generic and specific skills in robotic surgery.In virtual reality exercise,the scores of the 3 exercises increased significantly with every repetition(p<0.001)and the size of the increase was large.The completion time on the robot platform was 2.6 times faster(169.33±28.28 s vs.447.96±156.55 s,p<0.001)than that in the laparoscopic box,and the difference between both types of tests was large(pη2=0.797).The centralization and passage of the needle were significantly better on the robot platform(5 vs.3,p<0.001,r=0.47;5 vs.4,p<0.001,r=0.59)than in the laparoscopic box.For the intracorporeal stitch+knot test,every participant was able to perform the exercise on the robot but only 85.2%(23/27)in the laparoscopic box.Twenty-one participants answered the survey,and 13(61.9%)of them considered robotic performance independent of laparoscopic experience.Conclusions Surgeons are interested and seek training in robotic surgery.We implemented the first hands-on robotic surgery training program in Portugal and participants considered it was important and adequate for its purpose.All participants,even without robotic experience,learned quicker,performed better,faster and more precisely on the robot over laparoscopy. | Mario Rui Gonçalves Jose Novo de Matos Antonio Oliveira Ricardo Marinho Irene Cadime Palmira Carlos Alves Salvador Morales-Conde Miguel Castelo-Branco | 2023 | Laparoscopic, Endoscopic and Robotic Surgery2023，6，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员