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| 1 | N-9 Alkylation of purines via light-promoted and metal-free radical relay显示文摘A metal-free and light-promoted approach to the synthesis of N-9 alkylated purine nucleoside derivatives, via a CF3 radical triggered radical relay pathway, has been developed. Purine nucleoside derivatives were prepared regioselectively in good to high yields. Photosensitizers and metals are free in this transformation. Visible light or even sunlight can be used as the source of light for the reactions. | Runze Mao Lifeng Sun Yong-Shi Wang Min-Min Zhou De-Cai Xiong Qin Li Xin-Shan Ye | 2018 | Chinese Chemical Letters2018，29，1： | 1 |
| 2 | Engineering the viscoelasticity of gelatin methacryloyl(GelMA)hydrogels via small“dynamic bridges”to regulate BMSC behaviors for osteochondral regeneration显示文摘The dynamic extracellular matrix(ECM)constantly affects the behaviors of cells.To mimic the dynamics of ECM with controllable stiffness and energy dissipation,this study proposes a strategy in which a small molecule,3,4-dihydroxybenzaldehyde(DB),was used as fast'dynamic bridges'to construct viscoelastic gelatin methacryloyl(GelMA)-based hydrogels.The storage modulus and loss modulus of hydrogels were independently adjusted by the covalent crosslinking density and by the number of dynamic bonds.The hydrogels exhibited self-healing property,injectability,excellent adhesion and mechanical properties.Moreover,the in vitro results revealed that the viscous dissipation of hydrogels favored the spreading,proliferation,osteogenesis and chondrogenesis of bone marrow mesenchymal stem cells(BMSCs),but suppressed their adipogenesis.RNA-sequencing and immunofluorescence suggested that the viscous dissipation of hydrogels activated Yes-associated protein(YAP)by stabilizing integrinβ1,and further promoted nuclear translocation of smad2/3 andβ-catenin to enhance chondrogenesis and osteogenesis.As a result,the viscoelastic GelMA hydrogels with highest loss modulus showed best effect in cartilage and subchondral bone repair.Taken together,findings from this study reveal an effective strategy to fabricate viscoelastic hydrogels for modulating the interactions between cells and dynamic ECM to promote tissue regeneration. | Changjiang Liu Qifan Yu Zhangqin Yuan Qianping Guo Xiting Liao Feng Han Tao Feng Guoping Liu Runze Zhao Zhuang Zhu Haijiao Mao Caihong Zhu Bin Li | 2023 | Bioactive Materials2023，，7： | 1 |
| 3 | Phosphate-dependent regulation of vacuolar trafficking of OsSPX-MFSs is critical for maintaining intracellular phosphate homeostasis in rice显示文摘Vacuolar storage of inorganic phosphate(Pi)is essential for Pi homeostasis in plants.The SPX-MFS family proteins have been demonstrated to be vacuolar Pi transporters in many plant species.Transcriptional regulation of the predominant transporter among rice SPX-MFSs,OsSPX-MFS3,was only moderately suppressed by Pi starvation.Thus,post-transcriptional mechanisms were hypothesized to regulate the activity of OsSPX-MFS3.In this study,we found that the tonoplast localization of OsSPX-MFSs is inhibited under Pi-depleted conditions,resulting in their retention in the pre-vacuolar compartments(PVCs).A yeast two-hybrid screen identified that two SNARE proteins,OsSYP21 and OsSYP22,interact with the MFS domain of OsSPX-MFS3.Further genetic and cytological analyses indicate that OsSYP21 and OsSYP22 facilitate trafficking of OsSPX-MFS3 from PVCs to the tonoplast.Although a homozygous frameshift mutation in OsSYP22 appeared to be lethal,tonoplast localization of OsSPX-MFS3 was significantly inhibited in transgenic plants expressing a negative-dominant form of OsSYP22(OsSYP22-ND),resulting in reduced vacuolar Pi concentrations in OsSYP22-ND plants.Under Pi-depleted conditions,the interaction between OsSYP22 and OsSPX-MFS3 was disrupted,and this process depended on the presence of the SPX domain.Deleting the SPX domains of OsSPX-MFSs resulted in their tonoplast localization under both Pi-depleted and Pi-replete conditions.Complementation of the osspx-mfs1/2/3 triple mutants with the MFS domain or the SPX domain of OsSPX-MFS3 confirmed that the MFS and SPX domains are responsive to Pi transport activity and Pi-dependent regulation,respectively.These data indicated that the SPX domains of OsSPX-MFSs sense cellular Pi(InsP)levels and,under Pi-depleted conditions,inhibit the interaction between OsSPX-MFSs and OsSYP21/22 and subsequent trafficking of OsSPX-MFSs from PVCs to the tonoplast. | Runze Guo Qi Zhang Kun Qian Yinghui Ying Wenying Liao Lening Gan Chuanzao Mao Yong Wang James Whelan Huixia Shou | 2023 | Molecular Plant2023，16，8： | 0 |
| 4 | Bio-inspired Small Molecular Catalysis显示文摘What is the most favorite and original chemistry developed in your researchgroup?I hope it's always the next one.How do you get into this specific field?Could you please share some experiences with ourreaders?Nature provides many astonishing catalytic machineries for building up molecular complexity and harnessing energy in the most efficient ways.Nature's recipe for catalysis serves as a starting point to develop new catalyst for synthetic and energy chemistry. | Mao Cai Runze Zhang Chunming Yang Sanzhong Luo | 2023 | Chinese Journal of Chemistry2023，41，5： | 0 |
| 5 | Advances in 3D Bioprinting显示文摘Three-dimensional(3D)bioprinting has emerged as a promising approach for engineering functional tissues and organs by layer-by-layer precise positioning of biological materials,living cells,and biochemical components.Compared with nonbiological printing,3D bioprinting involves additional complexities and technical challenges owing to the processing of living cells,such as the appropriate biomaterials that fulfill the requirements for both printability and functionality.In this review,we first introduce the development course of 3D bioprinting,highlighting innovative forms of living building blocks and advances in enabling techniques of 3D bioprinting.We then summarize the state-of-the-art advancements in 3D bioprinting for biomedical applications,including macroscale tissue or organ bioprinting,disease modeling,microphysiological systems,biobots,and bioprinting in space.Despite the rapid development of 3D bioprinting over the past decades,most 3D bioprinted tissue or organ constructs are still far from being suitable for clinical translation,and it is necessary for the field of bioprinting to shift its focus from shape mimicking towards functionality development.Therefore,we provide our perspectives on this burgeoning field with an emphasis on functional maturation post printing and translational applications at the bedside. | Yongcong Fang Yuzhi Guo Tiankun Liu Runze Xu Shuangshuang Mao Xingwu Mo Ting Zhang Liliang Ouyang Zhuo Xiong Wei Sun | 2022 | Chinese Journal of Mechanical Engineering(Additive Manufacturing Frontiers)2022，1，1： | 0 |
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