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| 1 | Cloning of first abc transporter encoding gene from Trichoderma spp. and its expression during stress and mycoparasitism显示文摘Trichoderma in its natural environment competes for nutrient uptake and is required to protect itself from adverse natural toxic compounds, such as those produced by plants and other microbes in the soil community, or synthetic toxic compounds released human activity. One of the most important metabolic pathways for drug resistance and substrate uptake, both in prokaryotes and eukaryotes, is ATP dependent. The role of ABC transporter proteins in the biology of Trichoderma is still not known. We present the cloning of the first four ABC transporter genes (TABC1, TABC2, TABC3, TABC4 ) in Trichoderma, and in particular T. atroviride P1, and the characterization of TABC2 The complete sequence of this gene is 6535 bp, which includes a promoter of 1624 bp, a terminator of 642 bp and a coding region of 4264 bp. The promoter contains many of the potential transcription factor binding sites found in the 5’ upstream region of the ech42 gene of T. atroviride P1. These included: heat shock factors (HSF), a nitrogen-regulating factor (Nit-2), a stress-response element (STRE), a GCR1 elements, and a Cre BP1 motif. Northern analysis and RT-PCR demonstrated that TABC2 is highly expressed when Trichoderma is subjected to nitrogen starvation, grown in the presence of culture filtrates of Botrytis cinerea, Rhizoctonia solani, and Pythium ultimum, or when N-acetylglucosamine is added to the substrate. TABC2 appears to be co-regulated with some CWDE-encoding genes, suggesting that this is the first ABC transporter encoding gene involved in mycoparasitic events. It’s role in the interaction of Trichoderma with fungal hosts or plants is being investigated by targeted gene disruption and overexpression. | Lanzuise S Ruocco M Scala V Catapano L Woo S Ciliento R Ferraioli S Soriente I Vinale F Scala F Del Sorbo G Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 3 | Expression and localization of epidermal growth factor, transforming growth fac- tor-alpha,and localization of their common receptor in fetal human lung development 显示文摘 | Ruocco S LaUemand A Toumier JM | 1996 | Pediatr Res1996，39，3： | 1 |
| 4 | Endothelin 1 is overexpressed in human cirrhotic liver and exerts multiple effects on activated hepatic stellate cells显示文摘 | M Pinzani S Milani R De Franco C Grappone A Caligiuri A Gentilini C Tosti- Guerra M Maggi P Failli C Ruocco P Gentilini | 1996 | Gastroenterology1996，，2： | 1 |
| 5 | The molecular biology of the interactions between Trichoderma spp,phytopathogenic fungi,and plants显示文摘 | Woo S L Scala F Ruocco M | 2006 | Phytopathology2006，96，： | 1 |
| 6 | Four potato(Solanum tuberosum)ABCG transporters and their expression in response to abiotic factors and phytophthora infestans infection显示文摘 | Ruocco M Ambrosinob P Lanzuise S | 2011 | Journal of Plant Physiol2011，168，18： | 1 |
| 7 | Drug-induced pemphigus 显示文摘 | Brenner S Bialy-Golan A Ruocco V | 1998 | Clin Derma/ol1998，16，3： | 1 |
| 8 | Treatment of cereal products with a tailored preparation of trichoderma enzymes increases the amount of soluble dietary fiber显示文摘 | NAPOLITANO A LANZUISE S RUOCCO M | | 0，，20： | 1 |
| 9 | A case - contr ol study显示文摘 | POSTIGLIONE A MILAN G RUOCCO A Plasma folate vitamin B12 and total homocysteine and homozygosity for the C677T mutation of the 5 102 methylene tetrahydr ofolate reduetase gene in patientswithAlzheimer' s dementia | 2001 | Gerontology2001，47，： | 1 |
| 10 | Drug-induced pemphigus 显示文摘 | Brenner S Bialy-Golan A Ruocco V | 1998 | Clin Dermatol1998，16，3： | 1 |
| 11 | Isotopic response显示文摘 | Wolf R Brenner S Ruocco V | | 0，，05： | 1 |
| 12 | Experiences in threading UML throughout a computer science program显示文摘 | RUOCCO A S | 2003 | IEEE Transactions on Education2003，46，2： | 1 |
| 13 | Immunohistochemical localization of cystic fibrosis transmembrane conductance regulator in human fetal airway and digestive mucosa显示文摘 | Ruocco S Lallemand A | 1994 | Ped Res1994，36，2： | 1 |
| 14 | CCR7 signalling as an essential regulator of CNS infiltration in T- cell leukaemia显示文摘 | Buonamici S Trimarchi T Ruocco MG | 2009 | Nature2009，459，7249： | 1 |
| 15 | Comparison between eosinophilic markers in induced sputum and blood in asthmatic patients显示文摘 | Cianchetti S Ruocco L | 1998 | Clin Exp Allergy1998，28，10： | 1 |
| 16 | Pyoderma Pangrenosum : a review 显示文摘 | Ruocco E Sangiuliano S Gravina A G | 2009 | J Eur A cad Dermatol Venereol2009，23，9： | 1 |
| 17 | Analysis of a ther- moelectrical device for active heat transfer control 显示文摘 | MARUYAMA S NINO E RUOCCO G | 2001 | In- ternational Journal of Thermal Sciences2001，40，10： | 1 |
| 18 | 查看详情显示文摘 | Conti C Fratalocchi A Peccianti M Ruocco G Trillo S | | 0，，： | 1 |
| 19 | Drug-induced pemphigus显示文摘 | Brenner S Bialy-Golan A Ruocco V | | 0，，： | 1 |
| 20 | T helper 9 cells induced by plasmacytoid dendritic cells regulate interleukin-17 in multiple sclerosis显示文摘 | Ruocco G Rossi S Motta C | 2015 | Clin Sci2015，129，4： | 1 |
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