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您的检索式：作者名="Ruping Zhao"
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| 1 | Capilliposide from Lysimachia capillipes promotes terminal differentiations and reverses paclitaxel resistance in A2780T cells of human ovarian cancer by regulating Fos/Jun pathway显示文摘Objective:To investigate the potential effect of Lysimachia capillipes capilliposide(LCC)on the chemo sensitivity and the stemness of human ovarian cancer cells.Methods:Cell Counting Kit-8(CCK8)was used to measure the IC50 values.The apoptosis of cells was measured through flow cytometry.Evaluation of the stemness and differentiation markers was performed by the immunoblotting and the immunostaining assays.RNA-seq was performed through the Illumina HiSeq PE150 platform and differentially expressed genes(DEGs)were screened out through the bioinformation analysis.Overexpression or knockdown of Fos gene was achieved by shRNA transfection.Results:Pre-exposure of A2780T cells with 10μg/mL LCC sensitized them to paclitaxel,of which the IC50 value reduced from 8.644μmol/L(95%CI:7.315–10.082μmol/L)to 2.5μmol/L(95%CI:2.233–2.7882μmol/L).Exposure with LCC enhanced the paclitaxel-induced apoptosis and inhibited the colony formation of A2780T cells.LCC exposure reduced the expression of cancer stemness markers,ALDH1,Myd88 and CD44,while promoting that of terminal differentiation markers,NFATc1,Cathepsin K and MMP9.RNAseq analysis revealed that the expressions of FOS and JUN were upregulated in LCC-treated A2780T cells.A2780T cells overexpressing Fos gene displayed increased paclitaxel-sensitivity and reduced cell stemness,and shared common phenotypes with LCC-treated A2780T cells.Conclusion:These findings suggested that LCC promoted terminal differentiations of ovarian cancer cells and sensitized them to paclitaxel through activating the Fos/Jun pathway.LCC might become a novel therapy that targets at cancer stem cells and enhances the chemotherapeutic effect of ovarian cancer treatments. | Ke Zhang Hanyue Ying Ruping Zhao Yuanyuan Chen Qinghua Deng | 2022 | Chinese Herbal Medicines2022，14，1： | 0 |
| 2 | Unique capability of NdPO_(4)to activate hydrogen for efficient hydrogenation of furfural to furfuryl alcohol over Nd-Co-P composites显示文摘Efficient and applicable catalysts are highly desirable for advanced biomass transformation industry,here,we fabricated a series of Nd-Co-P catalysts for hydrogenation of furfural(FAL)to furfuryl alcohol(FOH).By comprehensive characterizations,it is demonstrated that the prepared Nd-Co-P samples are structured as(NdPO_(4))_(m)/Co_(2)P nanocomposites with molar ratio(m)in range of 0.24-1.1;by manipulating m,the outstanding catalytic efficiency comparable with the performance of precious metal catalysts,such as turnover frequency(TOF)up to 0,50 s^(-1)(being ten-fold higher than Co_(2)P)and FOH yield up to97%,is achieved on the reusable(NdPO_(4))_(m)/Co_(2)P composites.Te mperature programmed desorption(TPD),in-situ infrared spectroscopy(IR)and kinetic-mechanism studies further disclose that Co_(2)P is decisive for activating FAL,instead,NdPO_(4)possesses unique capability of activating hydrogen which readily facilitate the selective hydrogenation of FAL to FOH through a rapid Langmuir-Hinshelwood process over(NdPO_(4))_(m)/Co_(2)P catalyst.These results indicate that rare-earth phosphates like NdPO_(4)can act as the promising and reliable catalytic component to activate hydrogen,which can be of interest not only for innovating novel and applicable non-metallic catalysts for sustainable biomass transformation analogous to hydrogenation of FAL,but also for expanding material base for other green mass transformation techniques involving hydrogen. | Xianghua He Ben Dai Yafang Zhang Dan Zhao Dihui Zhang Ruping Liang Chao Chen | 2022 | Journal of Rare Earths2022，40，7： | 0 |
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