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| 1 | Homogeneous label-free fluorescent assay of small molecule-protein interactions using protein binding-inhibited transcription nanomachine显示文摘Quantitative analysis of interactions between small molecules and proteins is a central challenge in chemical genetics, molecular diagnostics and drug developments. Here, we developed a RNA transcription nanomachine by assembling T7 RNA polymerase on a small molecule-labeled DNA heteroduplex. The nanomachine, of which the RNA transcription activity can be quantitatively inhibited by protein binding, showed a great potential for small molecule-protein interaction assay. This finding enabled us to develop a novel homogeneous label-free strategy for assays of interactions between small molecules and their protein receptors. Three small molecule compounds and their protein receptors have been used to demonstrate the developed strategy. The results revealed that the protein-small molecule interaction assay strategy shows dynamic responses in the concentration range from 0.5 to 64 nM with a detection limit of 0.2 nM. Due to its label-free, homogeneous, and fluorescence-based detection format, besides its desirable sensitivity this technique could be greatly robust, cost-efficient and readily automated, implying that the developed small molecule-protein interaction assay strategy might create a new methodology for developing intrinsically robust, sensitive and selective platforms for homogeneous protein detection. | ZHOU DianMing, WU YiDan, LIU Pei, BAI HaoTian, TANG LiJuan, YU RuQin & JIANG JianHui State Key Laboratory of Chemo/Bio-Sensing and Chemometrics College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China | 2011 | Science China Chemistry2011，54，8： | 4 |
| 2 | HPLC-DAD data coupled with second-order calibration method applied to food analysis: Simultaneous determination of six benzoylurea insecticides in various fruit samples by selecting time region of chromatogram显示文摘This work presents a novel application of second-order calibration based on self-weighted alternating trilinear decomposition(SWATLD)algorithm for analyzing the HPLC-DAD data.The proposed method makes it possible to simultaneously determine teflubenzuron,hexaflumuron,flufenoxuron,chlorfluazuron,diflubenzuron and benzoylurea in different fruit samples,i.e.pear,apple and banana,in the selected time region of chromatogram.The concentration,elution time and spectral information of these benzoylurea insecticides are selectively extracted from complex matrices even in the presence of unknown interferences.The root-mean-square error of prediction(RMSEP)and figures of merit,including sensitivity(SEN),selectivity(SEL)and limit of detection(LOD)are employed to access the performance of the method.The LODs obtained for these insecticides are within the range 0.017–0.26 ppm in pears,0.039–0.33 ppm in apples,0.041–0.44 ppm in bananas,respectively.Such a chemometrics-based protocol holds great potential to be extended as a promising alternative for more practical applications in food safety and quality monitoring. | QING XiangDong WU HaiLong NIE ChongChong LI Yong YAN XiuFang ZHANG XiHua YIN XiaoLi YU RuQin | 2013 | Science China Chemistry2013，56，11： | 2 |
| 3 | Rapid determination of thiabendazole in orange extract using excitation-emission matrix fluorescence and second-order calibration based on alternating trilinear decomposition/alternating normalization-weighted error algorithms显示文摘A novel approach is proposed for direct quantitative analysis of thiabendazole in the orange extract by using excitation-emission matrix fluorescence coupled with second-order calibration methods based on the alternating trilinear decomposition(ATLD) and the alternating normalization-weighted error(ANWE) algorithms,respectively. The average recoveries of thiabendazole in the orange extract by using ATLD and ANWE with an estimated component number of two were 99.7 ± 3.3% and 103.5 ± 4.1%,respectively. Furthermore,the accuracy of the two algorithms was also evaluated through elliptical joint confidence region(EJCR) tests as well as figures of merit,such as sensitivity(SEN),selectivity(SEL) and limit of detection(LOD). The experimental results demonstrate that both algorithms have been satisfactorily applied to the determination of thiabendazole in orange extract,and the perform-ance of ANWE is slightly better than that of ATLD. | WANG XueMei WU HaiLong NIE JinFang LI YuanNa YU YongJie YU RuQin | 2008 | Science China Chemistry2008，51，8： | 2 |
| 4 | Interaction of epicatechin with bovine serum albumin using fluorescence quenching combined with chemometrics显示文摘The interaction between BSA and epicatechin was studied using fluorescence quenching titrations combined with trilinear decomposition method and excitation-emission matrix(EEM)fluorescence.The resolved spectra were highly similar with the actual ones which indicated that the resolved results were reliable.The relevant parameters of the binding process were obtained by quantifying each substance in the complicated mixtures in situ.The quenching was static quenching,epicatechin had a weak interaction with BSA and the binding site was one.The total concentration and the free concentration of quenchers had different effect on the system.The results demonstrated that the method exploited in this article is a useful tool to investigate complicated interactions,avoiding complicated pretreatment and simplify experimental procedure. | ZHAI Min WU HaiLong ZHANG ShuRong ZHANG XiHua SUN YanMei YU RuQin | 2014 | Science China Chemistry2014，57，5： | 2 |
| 5 | Microwave plasma treated carbon nanotubes and their electrochemical biosensing application显示文摘 | Zhaoyang Wu Yinyu Xu Xiaolei Zhang Guoli Shen Ruqin Yu | 2007 | Talanta2007，，4： | 1 |
| 6 | Similar distribution of simple sequence repeats in diverse completed Human lmmunodeficiency Virus Type 1 genomes显示文摘 | Ming Chen Zhongyang Tan Jianhui Jiang Mingfu Li Hongjun Chen and Ruqin Yu | 2009 | Federation of European Biochemical Societies2009，583，： | 1 |
| 7 | Amperometric glucose biosensor based on chitosan with improved selectivity and stability显示文摘 | Minghui Yang Yunhui Yang Bing Liu Guoli Shen Ruqin Yu | 2004 | Sensors & Actuators: B Chemical2004，，3： | 1 |
| 8 | Prediction of the Q-e parameters from transition state structures 显示文摘 | Xinliang Yu Ruqin Yu | 2013 | Polymer Engineering & Science2013，53，10： | 1 |
| 9 | A Reusable Piezo-Immunosensor with Amplified Sensitivity for Ceruloplasmin Based on Plasma-Polymerized Film显示文摘 | Hua Wang Dan Li Zhaoyang Wu Guoli Shen and Ruqin Yu | 2004 | Talanta2004，62，1： | 1 |
| 10 | Preparation and characterization of prussian blue nanowire array and bioapplication for glucose biosensing显示文摘 | QU Fengli SHI Aiwu YU Ruqin | 2007 | Anal Chim Acta2007，605，1： | 1 |
| 11 | A Protein A-Based Orientation-Controlled Immobilization Strategy for Antibodies Using Nanometer-Sized Gold Particles and Plasma-Polymerized Film显示文摘 | Hua Wang Yanli Liu Yunhui Yang Ting Deng Guoli Shen and Ruqin Yu | 2004 | Analytical Biochemistry2004，324，2： | 1 |
| 12 | Double strand DNA-templated copper nanoparticle as a novel fluorescence indicator for label-free detection of polynucleotide kinase activity显示文摘 | Liangliang Zhang Jingjin Zhao Hua Zhang Jianhui Jiang Ruqin Yu | 2013 | Biosensors and Bioelectronics2013，，： | 1 |
| 13 | Acetylcholinesterase liquid crystal biosensor for identification of AChE inhibitors by a reactivator显示文摘An improved acetylcholinesterase liquid crystal(LC) biosensor has been developed for the identification of organophosphates(OPs) by using a reactivator. When the acetylcholinesterases(AChEs) inhibited by different kinds of OPs are reactived by a reactivator, the catalytic activity of AChEs can be recovered with different activation efficiency because of the different phosphorylation structures formed in the inhibited AChEs. Accordingly, the reactived AChEs can catalyze the hydrolysis of acetylthiocholine to generate thiocholine product in different degrees, which will result in different catalytic growth of AuNPs and further form distinct orientational response of LCs. Based on such a reactivation mechanism, the AChE LC biosensor with a simple, rapid and visual procedure achieves an obvious identification of three OPs pesticides, methamidophos, trichlorfon and paraoxon, by using a pralidoxime reactivator. | DING HuaZhi LIAO ShuZhen XIAO FuBing SHEN GuoLi YU RuQin WU ZhaoYang | 2014 | Science China Chemistry2014，57，11： | 1 |
| 14 | Amperometric glucose biosensor based on chitosan with improved selectivity and stability显示文摘 | Minghui Yang Yunhui Yang Bing Liu Guoli Shen Ruqin Yu | 2004 | Sensors & Actuators: B. Chemical2004，，3： | 1 |
| 15 | Sequential number-theoretic optimization (SNTO) method applied to chemical quantitative analysis 显示文摘 | ZHANG Lin LIANG Yizeng YU Ruqin | 1998 | J Chemometrics1998，11，3： | 1 |
| 16 | Analysis of PAHs in air-borne particulates in Hong Kong City by heuristic evolving latent projections显示文摘 | Hailin Shen Yizeng Liang Ruqin Yu Xianchun Li Xinxi Sun | 1998 | Science in China Series B: Chemistry1998，，1： | 1 |
| 17 | A Chaotic Approach to Maintain the Population Diversity of Genetic Algorithm in Network Training 显示文摘 | Qingzhang Lu Guoli Shen Ruqin Yu | 2003 | Computational Biology and Chemistry2003，27，3： | 1 |
| 18 | Immobilization of horserad- ish peroxidase to a nano-Au monolayer modified chitosan-entrappedcarbon paste electrode for the detection of hydrogen peroxide显示文摘 | YU Ruqin LEI Cunxi HU Shunqin | 2003 | Talanta2003，59，： | 1 |
| 19 | Similar distribution of simple sequence repeats in diverse completed Human Immunodeficiency Virus Type 1 genomes显示文摘 | Ming Chen Zhongyang Tan Jianhui Jiang Mingfu Li Hongjun Chen Guoli Shen Ruqin Yu | 2009 | FEBS Letters2009，，17： | 1 |
| 20 | Enantioseleetive recognition of amino acid by differential pulse voltammetry in molecularly imprinted monolayers assembled on Au electrodes显示文摘 | HUANG Shuangyan SHENG Guoli YU Ruqin | 2004 | Electroanalysis2004，16，12： | 1 |
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