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| 1 | Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice显示文摘AIM To evaluate antihepatoma effect ofantisense phosphorothioate oligodeo-xyribonucleotides (S-ODNs) targeted to alpha-fetoprotein (AFP) genes in vitro and in nudemice.METHODS AFP gene expression was examinedby immunocytochemical method or enzyme-linked immunosorbent assay. Effect of S-ODNson SMMC-7721 human hepatoma cell growth invitro was determined using microculturetetrazolium assay. In vivo antitumor activitiesof S-ODNs were monitored by measuring tumorweight differences in treated and control micebearing SMMC-7721 xenografts. Induction of cellapoptosis was evaluated by fluorescence-activated cell sorter (FACS) analysis.RESULTS Antisense S-ODN treatment led toreduced AFP gene expression. Specificantisense S-ODNs, but not control S-ODNs,inhibited the growth of heaptoma cells in vitro.In vivo. only antisense S-ODNs exhibitedobvious antitumor activities. FACS analysisrevealed that the growth inhibition by antisenseS. ODNs was associated with their cell apoptosisinduction.CONCLUSION Antisense S-ODNs targeted toAFP genes inhibit the growth of human hepatomacells and solid hepatoma, which is related totheir cell apoptosis induction. | Xing Wang Wang~1 Jin Hui Yuan~1 Ru Gang Zhang~1 Li Xia Guo~1 Yong Xie~2 Hong Xie~1 ~1Department of Biotherapy,Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China ~2Department of Biology,Hong Kong University of Science and Technology,ChinaDr.Xing Wang Wang earned Ph.D.from Shanghai Institute of Materia Medical,Chinese Academy of Sciences in 1997.Now a professor at Shanghai Institute of Cell Biology,Chinese Academy of Sciences. | 2001 | World Journal of Gastroenterology2001，7，3： | 21 |
| 2 | Carbon Dioxide/Methane Separation by Adsorption on Sepiolite显示文摘在这个工作,为从由压力秋千吸附(PSA ) 的二氧化碳混合物的二氧化碳的移动的海泡石的使用过程被研究了。吸附平衡和动力学在一张改正床,和二氧化碳的吸附平衡参数被测量了,海泡石上的甲烷被获得了。一个模特儿基于 LDF 近似被雇用了模仿改正床动力学,用描述吸附平衡等温线的兰米尔方程。为作为吸附物用海泡石分开二氧化碳混合物一个 PSA 周期工作也被学习了。试验性的结果与使适应一个 PSA 系统的模型预言的相比。有比 97% 罐头高的纯净的甲烷被获得从喂包含二氧化碳,集中与建议 PSA 周期从 34% ~ 56%。这些结果建议那海泡石是有为它在为从垃圾堆气体的二氧化碳移动的一个 PSA 周期的雇用的好性质的吸附物。 | José A.Delgado María A.Uguina José L.Sotelo Beatriz Ruíz Marcio Rosário | 2007 | Journal of Natural Gas Chemistry2007，16，3： | 10 |
| 3 | Up-regulation of mitochondrial chaperone TRAP1 in ulcerative colitis associated colorectal cancer显示文摘AIM:To characterize tumor necrosis factor receptorassociated protein 1(TRAP1)expression in the progression of ulcerative colitis(UC)-associated colorectal cancer.METHODS:Chronic UC is an inflammatory bowel disease that predisposes to colorectal cancer.Immunohistochemical analysis was used to evaluate TRAP1expression on tissue microarrays containing colonic tissues from 42 UC progressors(patients with cancer or dysplasia)and 38 non-progressors(dysplasia/cancer free patients).Statistical analyses of the TRAP1immunohistochemistry staining were performed using Graph Pad Prism.Differences in the TRAP1 level between non-progressors and progressors were tested for statistical significance using the Mann-Whitney test.Receiver operating characteristic curve method was used to quantify marker performance in distinguishing diseased cases from controls.RESULTS:TRAP1 was up-regulated in the colon tissues from UC progressors,but not in the colon tissues from UC non-progressors.Moreover,up-regulation of TRAP1 preceded the neoplastic changes:it was present in both the dysplastic and non-dysplastic tissues of UC progressors.When TRAP1 staining in rectal tissue was used as a diagnostic marker,it could distinguish progressors from non-progressors with 59%sensitivity and 80%specificity.Our study further showed that the increase of TRAP1 expression positively correlated with the degree of inflammation in the colorectal cancer tissues,which could be related to the increased oxidation present in the colonic mucosa from UC progressors.We then investigated the cellular proteome changes underlying oxidative stress,and found that oxidative stress could induce up-regulation of TRAP1 along with several other negative modulators of apoptosis.CONCLUSION:These results suggest that oxidative stress in long standing UC could lead to the increase of cytoprotective protein TRAP1,which in turn could promote cancer progression by preventing or protecting the oxidative damaged epithelial cells from undergoing apoptosis.TRAP1 could be a potential diagnostic marker for UC associated colorectal cancer. | Ru Chen Sheng Pan Keith Lai Lisa A Lai David A Crispin Mary P Bronner Teresa A Brentnall | 2014 | World Journal of Gastroenterology2014，20，45： | 9 |
| 4 | Transretinoic acid inhibits rats gastric epithelial dysplasia induced by N-methyi-N-nitro-N-nitrosoguanidine:influences on cell apoptosis and expression of its regulatory genes显示文摘INTRODUCTIONGastric epithelial dysplasia (GED) hypothetically is a straight-forward concept: dysplastic epithelium replacing the normal gastric epithelium of the stomach [1].In the stomach ,like any other segment of the gut ,it is defined as an unequivocal non-invasive epithelial change[2,3].The observation of gastric dysplasia as a cancerous lesion was recognized over a century ago ,but it is only after the advent of gastroscopy that its clinical significance has been stressed[4-7]. | Ru Tao Cui~1 Gan Cai~2 Zhao Bao Yin~(2*) Yong Cheng~2 Qiu Hong Yang~1 Tao Tian~(3#) ~1Liver Center,Beijing Friendship Hospital Affiliated to CapitalUniversity of Medical Sciences~2Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai China and Department of Pathophysiology.Shanghai Medical University.Shanghai,China ~3Pathological Department,Shanghai Medical University (?)Now working at UCLA,USA ~#Now working at University of Michigan,USADr.Ru Tao Cui graduated and got the bachelor degree from Shandong University of Traditional Chinese Medicine in 1994,got doctor degree from Shanghai University of Traditional Chinese Medicine in 2000,now studying as a postdoc,majoring gastroenterology,having 29 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 5 | Residual activity of cetrimide and chlorhexidine on Enterococcus faecalis-infected root canals显示文摘Effective final irrigation regimen is an important step in order to achieve better disinfection and ensure residual antimicrobial effects after root canal preparation. The aim of this study was to compare the residual antimicrobial activity of 0.2% cetrimide, and 0.2% and 2% chlorhexidine in root canals infected with Enterococcus faecalis. Biofilms of E. faecalis were grown on uniradicular roots for 4 weeks. After root canal preparation, root canals were irrigated with 17% ethylenediaminetetraacetic acid(EDTA) to remove the smear layer. The roots were randomly divided into three experimental groups(n526) according to the final irrigating solution: Group I, 5 mL 0.2% cetrimide; Group II, 5 mL 0.2% chlorhexidine; and Group III, 5 mL 2% chlorhexidine. Samples were collected for 50 days to denote the presence of bacterial growth. The proportion of ungrown specimens over 50 days was evaluated using the nonparametric Kaplan–Meier survival analysis. Differences among groups were tested using the log-rank test and the level of statistical significance was set at P,0.05. The highest survival value was found with 2% chlorhexidine, showing statistically significant differences from the other two groups. At 50 days, E. faecalis growth was detected in 69.23% specimens in Groups I and II, and in 34.61% specimens of Group III. There were no significant differences between 0.2% cetrimide and 0.2% chlorhexidine. Final irrigation with 2% chlorhexidine showed greater residual activity than 0.2% chlorhexidine and 0.2% cetrimide in root canals infected with E. faecalis. | Carmen María Ferrer-Luque María Teresa Arias-Moliz Matilde Ruíz-Linares María Elena Martínez García Pilar Baca | 2014 | International Journal of Oral Science2014，6，1： | 5 |
| 6 | Glycoproteins and glycoproteomics in pancreatic cancer显示文摘Aberrations in protein glycosylation and polysaccharides play a pivotal role in pancreatic tumorigenesis, influencing cancer progression, metastasis, immunoresponse and chemoresistance. Abnormal expression in sugar moieties can impact the function of various glycoproteins, including mucins, surface receptors, adhesive proteins, proteoglycans, as well as their effectors and binding ligands, resulting in an increase in pancreatic cancer invasiveness and a cancerfavored microenvironment. Recent advance in glycoproteomics, glycomics and other chemical biology techniques have been employed to better understand the complex mechanism of glycosylation events and how they orchestrate molecular activities in genomics, proteomics and metabolomics implicated in pancreatic adenocarcinoma. A variety of strategies have been demonstrated targeting protein glycosylation and polysaccharides for diagnostic and therapeutic development. | Sheng Pan Teresa A Brentnall Ru Chen | 2016 | World Journal of Gastroenterology2016，22，42： | 3 |
| 7 | Effect of a bacterial inoculum and additive on dry matter in situ degradability of sugarcane silage显示文摘The objective of this study was to evaluate the effect of adding a bacterial inoculum and a handmade additive to sugarcane silage(SCS) on the in situ digestibility of dry matter(DM). The treatments were: T1) sugarcane silage(SCS) and T2) sugarcane silage with 1% inoculum and 1% additive(SCS+). The bacterial inoculum consisted of 10.0% molasses, 1.0% yogurt, 5.0% chicken manure, 0.5% urea, and 83.0% water, and the additive was formulated with 1.0% urea, 0.1% ammonium sulfate, and 0.25% phosphorus. In situ dry matter digestibility(DMD) was determined using the nylon bag technique with four cows equipped with ruminal fistulas. Cows were fed with ensiled sugarcane supplemented with 1 kg of commercial concentrate. 5 g of ground sample for each sugarcane treatment were weighted in nylon bags and incubated for 4, 8, 12, 24, 48, and 72 h in a completely randomized design with six replicates. The DMD(%) was higher(P<0.05) for SCS+ for all incubation times when compared with SCS. There were no differences in ruminal p H between the treatments for all the incubation times. The data suggested that the sugarcane silage with bacterial inoculum and additive could be an alternative for providing forage for ruminants during the season of low growth and quality grass. | José A Reyes-Gutiérrez Oziel D Montaez-Valdez Ramón Rodríguez-Macias Mario Ruíz-López Eduardo Salcedo-Pérez Cándido E Guerra-Medina | 2015 | Journal of Integrative Agriculture2015，14，3： | 3 |
| 8 | Biomarkers for colitis-associated colorectal cancer显示文摘Patients with extensive ulcerative colitis(UC) of more than eight years duration have an increased risk of colorectal cancer. Molecular biomarkers for dysplasia and cancer could have a great clinical value in managing cancer risk in these UC patients. Using a wide range of molecular techniques- including cutting-edge OMICS technologies- recent studies have identified clinically relevant biomarker candidates from a variety of biosamples, including colonic biopsies, blood, stool, and urine. While the challenge remains to validate these candidate biomarkers in multi-center studies and with larger patient cohorts, it is certain that accurate biomarkers of colitis-associated neoplasia would improve clinical management of neoplastic risk in UC patients. This review highlights the ongoing avenues of research in biomarker development for colitis-associated colorectal cancer. | Ru Chen Lisa A Lai Teresa A Brentnall Sheng Pan | 2016 | World Journal of Gastroenterology2016，22，35： | 2 |
| 9 | Intelligent field de- vices user expectations 显示文摘 | CAPETTA L MELLA A RU SSO F | 1995 | Camputing & Control Engineer- ing Journal1995，6，6： | 1 |
| 10 | Variation in nutritive value of commercial broiler diets 显示文摘 | Ru Y J Hughes R J Choct M Kruk J A | 2003 | Asian Australasian Journal of Animal Science2003，16，830： | 1 |
| 11 | Cincinnati pre- hospital stroke scale: reproducibility and validity 显示文摘 | Kothari RU Pancioli A Liu T | 1999 | Ann Emerg Med1999，33，4： | 1 |
| 12 | Listeria monoeytogenes infection in macrophages induces vacuolar-dependent host miRNA response显示文摘 | Sehnitger AK Machova A Mueller RU | 2011 | PLoS One2011，6，27： | 1 |
| 13 | Chloroplast microsatellite variation in tretaploid alfalfa显示文摘 | Mengoni A Ru ni C Vendramin G | 2000 | Plant Breeding2000，119，： | 1 |
| 14 | Anaphylactic shock reaction to dibutyl-phthalate-containing capsules显示文摘 | Gall H Kaehler A Peter RU | 1999 | Dermatology1999，199，： | 1 |
| 15 | The impact of absorptive capacity on technological acquisitions engineering consulting companies显示文摘 | M a del Carmen Haro-Domínguez Daniel Arias-Aranda Francisco Javier Lloréns-Montes Antonia Ruíz Moreno | 2007 | Technovation2007，，8： | 1 |
| 16 | Cincinnati Prehospital Stroke Scale:reproducibility and validity显示文摘 | Kothari RU Pancioli A Liu T | 1999 | Ann Emerg Med1999，33，4： | 1 |
| 17 | Mixed-methods resistance training increases power and strength of young and older men 显示文摘 | Newton RU Hakkinen K Hakkinen A | 2002 | Med Sci Sports Exere2002，34，8： | 1 |
| 18 | Update on the diagnosis and treatment of bladder endometriosis显示文摘 | Héctor Pastor-Navarro José M. Giménez-Bachs María J. Donate-Moreno José M. Pastor-Guzman Rafael Ruíz-Mondéjar Manuel Atienzar-Tobarra Antonio S. Salinas-Sánchez Julio A. Virserda-Rodriguez | 2007 | International Urogynecology Journal2007，，8： | 1 |
| 19 | Clinical significance of serum levels of IGF-1and IGFBP-3in children with Henoch-Schonlein purpura or Henoch-Schonlein purpura nephritis显示文摘 | Ru L Abudouhaer A Guo Y F | 2013 | Zhongguo Dang Dai Er Ke Za Zhi2013，15，11： | 1 |
| 20 | Synergistic Effect Between Two Bacteriocin-like Inhibitory Substances Produced by Lactobacilli Strains with Inhibitory Activity for Streptococcus agalactiae显示文摘 | Francisco O. Ruíz Gisela Gerbaldo María J. García Walter Giordano Liliana Pascual Isabel L. Barberis | 2012 | Current Microbiology2012，，4： | 1 |
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