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| 1 | 自发性脑出血治疗指南美国心脏协会/美国卒中协会对医疗卫生专业人员发布的指南显示文摘目的本指南旨在为急性自发性脑出血的诊断和治疗提供最新的综合性推荐意见。方法通过Medline进行规范的文献检索,利用证据表合并资料。撰写委员会成员通过远程电信会议讨论根据资料得出的推荐意见。采用美国心脏协会卒中委员会的证据分级方案对推荐意见进行分级。由6位同行评议专家以及卒中委员会科学声明监督委员会和卒中委员会领导委员会成员对指南的草案进行发表前审阅。预期本指南在3年内完全更新。结果本文为脑出血患者的医疗诊治提供了循证指南。重点包括诊断、止血、血压管理、院内管理和护理、预防内科合并症、外科治疗、转归预测、康复、预防复发以及将来需要考虑的问题。结论脑出血是一种严重的疾病,早期积极救治可影响其转归。本指南为脑出血患者的目标导向治疗提供了一个框架。 | Lewis B. Morgenstem J. Claude Hemphill Ⅲ Craig Anderson Kyra Becker Joseph P. Broderick E. Sander Connolly Jr Steven M. Greenberg James N. Huang R. Loch Macdonald Steven R. Messe Pamela H. Mitchell Magdy Selim Rafael J. Tamargo 王玉洁(译) 王健(译) 刘相玉(译) 谢丽丽(译) | 2010 | 国际脑血管病杂志2010，，8： | 259 |
| 2 | Neurodegeneration:An early event of diabetic retinopathy显示文摘Diabetic retinopathy(DR) has been classically considered to be a microcirculatory disease of the retina caused by the deleterious metabolic effects of hyperglycemia per se and the metabolic pathways triggered by hyperglycemia.However,retinal neurodegeneration is already present before any microcirculatory abnormalities can be detected in ophthalmoscopic examination.In other words,retinal neurodegeneration is an early event in the pathogenesis of DR which predates and participates in the microcirculatory abnormalities that occur in DR.Therefore,the study of the mechanisms that lead to neurodegeneration will be essential to identify new therapeutic targets in the early stages of DR.Elevated levels of glutamate and the overexpression of the renin-angiotensin-system play an essential role in the neurodegenerative process that occurs in diabetic retina.Among neuroprotective factors,pigment epithelial derived factor,somatostatin and erythropoietin seem to be the most relevant and these will be considered in this review.Nevertheless,it should be noted that the balance between neurotoxic and neuroprotective factors rather than levels of neurotoxic factors alone will determine the presence or absence of retinal neurodegeneration in the diabetic eye.New strategies,based on either the delivery of neuroprotective agents or the blockade of neurotoxic factors,are currently being tested in experimental models and in clinical pilot studies.Whether these novel therapies will eventually supplement or prevent the need for laser photocoagulation or vitrectomy awaits the results of additional clinical research. | Marta Villarroel Andreea Ciudin Cristina Hernández Rafael Simó | 2010 | World Journal of Diabetes2010，1，2： | 41 |
| 3 | Overlapping expression of microRNAs in human embryonic colon and colorectal cancer显示文摘MicroRNAs (miRNAs ) 为调整房间区别并且维持干细胞的 pluripotent 状态是必要的。尽管特定的 miRNAs 的 dysregulation 与癌症的某些类型被联系了,到日期,没有证据连接了 miRNA 表示在胚胎并且肿瘤纸巾。我们在人的胚胎的结肠组织,并且在 colorectal 癌症估计了成熟 miRNAs 的表示并且配对正常结肠组织。重叠 miRNA 表示在胚胎的结肠的 mucosa 和 colorectal 癌症之间被检测。我们发现了 miR-17-92 聚类,调整在两个盒子中的房间增长并且它的目标, E2F1,在人的冒号开发和结肠的 carcinogenesis 展出表示的一个类似的模式。在 situ,杂交在地窟祖先分隔空间证实了 miR-17-5p 的表示的高水平。我们断定 miRNA 小径在胚胎的开发和结肠的上皮的肿瘤的转变起一个主要作用。 | Mariano Monzo Alfons Navarro Eva Bandres Rosa Artells Isabel Moreno Bemat Gel Rafael Ibeas Jose Moreno Francisco Martinez Tania Diaz Antonio Martinez Olga Balague Jesus Garcia-Foncillas | 2008 | Cell Research2008，18，8： | 32 |
| 4 | 美国临床生化科学院检验医学实践指南:睾丸、前列腺、结直肠、乳腺及卵巢癌肿瘤标志物的应用显示文摘经美国临床生化科学院(NCAB)授权,由鄢盛恺教授组织学者、专家翻译审定2009年NCAB检验医学实践指南:睾丸、前列腺、结直肠、乳腺及卵巢癌中肿瘤标志物的应用单行本(Catharine M.Sturgeon教授和Eleftherios P.Diaman-dis教授编辑)。该指南包含第1至6章共6部分内容,参加指南制订的专家阵容强大,分别从临床及检验角度对睾丸、前列腺、结直肠、乳腺及卵巢癌中肿瘤标志物的检测与临床应用方面的问题进行了详细阐述与说明,是目前国内外在肿瘤实验诊断方面的纲领性应用文件。不仅适合广大检验人员、临床医护人员学习使用,也非常适合相关仪器试剂生产厂商研发应用。本指南由刘洋、薛丽、林文涛、李江、梁红艳、顾兵、潘世扬、郑磊等翻译,姜晓峰、沈文梅、田亚平、鄢盛恺审校,最后由鄢盛恺统稿审定。本指南的翻译出版,不仅有助于我国检验人员和医护人员合理的检测和应用睾丸、前列腺、结直肠、乳腺及卵巢癌肿瘤标志物,对我国检验医学指南的编写与应用也有一定的借鉴作用。本刊特在本期刊发,以飨广大读者。 | 鄢盛恺 Ulf-Hkan Stenman Rolf Lamerz Leendert H.Looijenga Nils Brünner Michael J.Duffy Caj Haglund Mads Holten-Andersen Hans JФrgen Nielsen Francisco J.Esteva Nadia Harbeck Daniel F.Hayes Rafael Molina Daniel W.Chan Robert C.Bast Jr Ie-Ming Shih Lori J.Sokoll Gyrgy Slétormos 刘洋 梁红艳 姜晓峰 田亚平 薛丽 林文涛 顾兵 潘世扬 沈文梅 郑磊 李江 | 2012 | 临床检验杂志2012，30，2： | 32 |
| 5 | A Technical Review of Hydro-Project Development in China显示文摘This paper summarizes the development of hydro-projects in China,blended with an international perspective.It expounds major technical progress toward ensuring the safe construction of high dams and river harnessing,and covers the theorization of uneven non-equilibrium sediment transport,inter-basin water diversion,giant hydro-generator units,pumped storage power stations,underground caverns,ecological protection,and so on. | Rafael Duarte António Pinheiro Anton J. Schleiss | 2016 | Engineering2016，2，3： | 31 |
| 6 | Intestinal microbiota in health and disease: Role of bifidobacteria in gut homeostasis显示文摘The pool of microbes inhabiting our body is known as 'microbiota' and their collective genomes as 'microbiome'. The colon is the most densely populated organ in the human body, although other parts, such as the skin, vaginal mucosa, or respiratory tract, also harbour specific microbiota. This microbial community regulates some important metabolic and physiological functions of the host, and drives the maturation of the immune system in early life, contributing to its homeostasis during life. Alterations of the intestinal microbiota can occur by changes in composition(dysbiosis), function, or microbiota-host interactions and they can be directly correlated with several diseases. The only disease in which a clear causal role of a dysbiotic microbiota has been demonstrated is the case of Clostridium difficile infections. Nonetheless, alterations in composition and function of the microbiota have been associated with several gastrointestinal diseases(inflammatory bowel disease, colorectal cancer, or irritable bowel syndrome), as well as extra-intestinal pathologies, such as those affecting the liver, or the respiratory tract(e.g., allergy, bronchial asthma, and cystic fibrosis), among others. Species of Bifidobacterium genus are the normal inhabitants of a healthy human gut and alterations in number and composition of their populations is one of the most frequent features present in these diseases. The use of probiotics, including bifidobacteria strains, in preventive medicine to maintain a healthy intestinal function is well documented. Probiotics are also proposed as therapeutic agents for gastrointestinal disorders and other pathologies. The World Gastroenterology Organization recently published potential clinical applications for several probiotic formulations, in which species of lactobacilli are predominant. This review is focused on probiotic preparations containing Bifidobacterium strains, alone or in combination with other bacteria, which have been tested in human clinical studies. In spite of extensive literature on and research into this topic, the degree of scientific evidence of the effectiveness of probiotics is still insufficient in most cases. More effort need to be made to design and conduct accurate human studies demonstrating the efficacy of probiotics in the prevention, alleviation, or treatment of different pathologies. | Rafael Tojo Adolfo Suárez Marta G Clemente Clara G de los Reyes-Gavilán Abelardo Margolles Miguel Gueimonde Patricia Ruas-Madiedo | 2014 | World Journal of Gastroenterology2014，20，41： | 33 |
| 7 | Nat Methods:新型计算方法或能更加精准地分析基因表达状况显示文摘日前,一项刊登在国际杂志NaCre Methods上的研究报告中,来自美国卡内基梅隆大学等机构的研究人员通过研究开发了一种新型的计算方法,该方法能够有效改善对基因表达分析的准确性。研究人员或有望利用该方法来诊断并且监测癌症。同时或许也能够促进基础生物学的研究进展。 | Rob Patro Geet Duggal Michael I Love Rafael A Irizarry Michael I Love Rafael A Irizarry Carl Kingsford | 2017 | 现代生物医学进展2017，17，11： | 30 |
| 8 | Corporate Ownership Around the World显示文摘 | Rafael LaPorta FlorencioLopez‐De‐Silanes AndreiShleifer | 2002 | The Journal of Finance2002，，2： | 24 |
| 9 | Advances in immunotherapy for treatment of lung cancer显示文摘Different approaches for treating lung cancer have been developed over time, including chemotherapy, radiotherapy and targeted therapies against activating mutations. Lately, better understanding of the role of the immunological system in tumor control has opened multiple doors to implement different strategies to enhance immune response against cancer cells. It is known that tumor cells elude immune response by several mechanisms. The development of monoclonal antibodies against the checkpoint inhibitor programmed cell death protein 1(PD-1) and its ligand(PD-L1), on T cells, has led to high activity in cancer patients with long lasting responses. Nivolumab, an anti PD-1 inhibitor, has been recently approved for the treatment of squamous cell lung cancer patients, given the survival advantage demonstrated in a phase III trial. Pembrolizumab, another anti PD-1 antibody, has received FDA breakthrough therapy designation for treatment of non-small cell lung cancer(NSCLC), supported by data from a phase I trial. Clinical trials with anti PD-1/PD-L1 antibodies in NSCLC have demonstrated very good tolerability and activity, with response rates around 20% and a median duration of response of 18 months. | Jean G.Bustamante Alvarez María González-Cao Niki Karachaliou Mariacarmela Santarpia Santiago Viteri Cristina Teixidó Rafael Rosell | 2015 | Cancer Biology & Medicine2015，12，3： | 22 |
| 10 | China’s 1-3-7 surveillance and response strategy for malaria elimination: Is case reporting, investigation and foci response happening according to plan?显示文摘Background:The China’s 1-3-7 strategy was initiated and extensively adopted in different types of counties(geographic regions)for reporting of malaria cases within 1 day,their confirmation and investigation within 3 days,and the appropriate public health response to prevent further transmission within 7 days.Assessing the level of compliance to the 1-3-7 strategy at the county level is a first step towards determining whether the surveillance and response strategy is happening according to plan.This study assessed if the time-bound targets of the 1-3-7 strategy were being sustained over time.Such information would be useful to improve implementation of the 1-3-7 strategy in China.Methods:This cross-sectional study involved country-wide programmatic data for the period January 1st 2013 to June 30th 2014.Data variables were extracted from the national malaria information system and included socio-demographic information,type of county,date of diagnosis,date of reporting,date of case investigation,case classification(indigenous,or imported,or unknown),focus investigation,date of reactive case detection(RACD),and date of indoor residual spraying(IRS).Summary statistics and proportions were used and comparisons between groups were assessed using the chi-square test.Level of significance was set at a P-value≤0.05.Results:Of a total of 5,688 malaria cases from 731 counties,there were 55(1%)indigenous cases(only in Type 1 and Type 2 counties)and 5,633(99%)imported cases from all types of counties.There was no delay in reporting malaria cases by type of county.In terms of case investigation,97.5%cases were investigated within 3 days with the proportion of delays(1.5%)in type 2 counties,being significantly lower than type 1 counties(4.1%).Regarding active foci,96.4%were treated by RACD and/or IRS.Conclusions:The performance of 1-3-7 strategy was encouraging but identified some challenges that if addressed can further improve implementation. | Shui-Sen Zhou Shao-Sen Zhang Li Zhang Aafje ECRietveld Andrew RRamsay Rony Zachariah Karen Bissell Rafael Van den Bergh Zhi-Gui Xia Xiao-Nong Zhou Richard ECibulskis | 2015 | Infectious Diseases of Poverty2015，4，1： | 21 |
| 11 | Long-term outcomes of hepatocellular carcinoma that underwent chemoembolization for bridging or downstaging显示文摘BACKGROUND Prospective study of 200 patients with hepatocellular carcinoma(HCC)that underwent liver transplant(LT)after drug-eluting beads transarterial chemoembolization(DEB-TACE)for downstaging versus bridging.Overall survival and tumor recurrence rates were calculated,eligibility for LT,time on the waiting list and radiological response were compared.After TACE,only patients within Milan Criteria(MC)were transplanted.More patients underwent LT in bridging group.Five-year post-transplant overall survival,recurrence-free survival has no difference between the groups.Complete response was observed more frequently in bridging group.Patients in DS group can achieve posttransplant survival and HCC recurrence-free probability,at five years,just like patients within MC in patients undergoing DEB-TACE.AIM To determine long-term outcomes of patients with HCC that underwent LT after DEB-TACE for downstaging vs bridging.METHODS Prospective cohort study of 200 patients included from April 2011 through June 2014.Bridging group included patients within MC.Downstaging group(out of MC)was divided in 5 subgroups(G1 to G5).Total tumor diameter was≤8 cm for G1,2,3,4(n=42)and was>8 cm for G5(n=22).Downstaging(n=64)and bridging(n=136)populations were not significantly different.Overall survival and tumor recurrence rates were calculated by the Kaplan-Meier method.Additionally,eligibility for LT,time on the waiting list until LT and radiological response were compared.RESULTS After TACE,only patients within MC were transplanted.More patients underwent LT in bridging group 65.9%(P=0.001).Downstaging population presented:higher number of nodules 2.81(P=0.001);larger total tumor diameter 8.09(P=0.001);multifocal HCC 78%(P=0.001);more post-transplantation recurrence 25%(P=0.02).Patients with maximal tumor diameter up to 7.05 cm were more likely to receive LT(P=0.005).Median time on the waiting list was significantly longer in downstaging group 10.6 mo(P=0.028).Five-year posttransplant overall survival was 73.5%in downstaging and 72.3%bridging groups(P=0.31),and recurrence-free survival was 62.1%in downstaging and 74.8%bridging groups(P=0.93).Radiological response:complete response was observed more frequently in bridging group(P=0.004).CONCLUSION Tumors initially exceeding the MC down-staged after DEB-TACE,can achieve post-transplant survival and HCC recurrence-free probability,at five years,just like patients within MC in patients undergoing DEB-TACE. | Breno Boueri Affonso Francisco Leonardo Galastri Joaquim Mauricio da Motta Leal Filho Felipe Nasser Priscila Mina Falsarella Rafael Noronha Cavalcante Marcio Dias de Almeida Guilherme Eduardo Goncalves Felga Leonardo Guedes Moreira Valle Nelson Wolosker | 2019 | World Journal of Gastroenterology2019，25，37： | 21 |
| 12 | Elastography-based screening for esophageal varices in patients with advanced chronic liver disease显示文摘Elastography-based liver stiffness measurement(LSM) is a non-invasive tool for estimating liver fibrosis but also provides an estimate for the severity of portal hypertension in patients with advanced chronic liver disease(ACLD). The presence of varices and especially of varices needing treatment(VNT) indicates distinct prognostic stages in patients with compensated ACLD(cACLD). The Baveno VI guidelines suggested a simple algorithm based on LSM < 20 kPa(by transient elastography, TE) and platelet count > 150 G/L for ruling-out VNT in patients with cACLD. These(and other) TE-based LSM cut-offs have been evaluated for VNT screening in different liver disease etiologies. Novel point shear-wave elastography(pSWE) and two-dimensional shear wave elastography(2D-SWE) methodologies for LSM have also been evaluated for their ability to screen for 'any' varices and for VNT. Finally, the measurement of spleen stiffness(SSM) by elastography(mainly by pSWE and 2D-SWE) may represent another valuable screening tool for varices. Here, we summarize the current literature on elastography-based prediction of 'any' varices and VNT. Finally,we have summarized the published LSM and SSM cut-offs in clinically useful scale cards. | Rafael Paternostro Thomas Reiberger Theresa Bucsics | 2019 | World Journal of Gastroenterology2019，25，3： | 20 |
| 13 | Effects of creep feeding and supplemental glutamine or glutamine plus glutamate (Aminogut) on pre- and post-weaning growth performance and intestinal health of piglets显示文摘Background: Creep feeding is used to stimulate piglet post-weaning feed consumption.L-Glutamine(GLN) is an important source of fuel for intestinal epithelial cells.The objective of this study was to determine the impact of creep feeding and adding GLN or AminoGut(AG;containing glutamine + glutamate) to pre-and post-weaning diets on pig performance and intestinal health.Litters(N = 120) were allotted to four treatments during 14–21 d of lactation: 1) No creep feed(NC,n = 45);2) creep fed control diet(CFCD,n = 45);3) creep fed 1% GLN(CFGLN,n = 15);4) creep fed.88% AG(CFAG,n = 15).After weaning,the NC and CFCD groups were sub-divided into three groups(n = 15 each),receiving either a control nursery diet(NC-CD,CFCD-CD) or a diet supplemented with either GLN(NC-GLN,CFCD-GLN) or with AG(NC-AG,CFCD-AG).Litters that were creep fed with diets containing GLN or AG also were supplemented with those amino acids in the nursery diets(CFGLN-GLN,CFAG-AG).Glutamine was added at 1% in all three post-weaning diet phases and AG was added at.88% in phase 1 and 2 and at.66% in phase 3.Results: Feed conversion(feed/gain) showed means among treatment means close to significance(P = 0.056) and Tukey's test for pairwise mean comparisons showed that Pigs in the CFGLN-GLN group had the best feed conversion(feed/gain) in the first three-week period post-weaning,exceeding(P = 0.044) controls(CFCD-CD) by 34%.The NC-AG group had(P = 0.02) the greatest feed intake in the last three week of the study,exceeding controls(CFCD-CD) by 12%.CFGLN-GLN,CFCD-GLN and sow reared(SR) pigs had the greatest(P = 0.049) villi height exceeding the CFCD-AG group by 18%,20% and 19% respectively.The CFAG-AG group had the deepest(P = 0.001) crypts among all treatments.CFGLN-GLN,CFCD-GLN and SR groups had the greatest(P = 0.001) number of cells proliferating(PCNA) exceeding those in the NC-CD group by 43%,54% and 63% respectively.Sow reared pigs showed the greatest(P = 0.001) intestinal absorption capacity for xylose and mannitol.Conclusion: Supplementation of creep feed and nursery diets with GLN and/or AminoGut in the first three week improved feed conversion possibly due to improved intestinal health. | Rafael A Cabrera James L Usry Consuelo Arrellano Eduardo T Nogueira Marianne Kutschenko Adam J Moeser Jack Odle | 2013 | Journal of Animal Science and Biotechnology2013，4，3： | 17 |
| 14 | Investor protection and corporate governance显示文摘 | Rafael La Porta Florencio Lopez-de-Silanes Andrei Shleifer Robert Vishny | 2000 | Journal of Financial Economics2000，，1： | 16 |
| 15 | 鳞状上皮细胞癌抗原水平新检测方法在宫颈癌、肺癌和头颈部癌鉴别诊断中的性能及临床表现显示文摘鳞状上皮细胞癌抗原水平在鳞状上皮细胞癌中通常升高。这项多中心研究评估了一种新的Elecsys?鳞状上皮细胞癌检测的检测性能,该检测方法是以等摩尔方式测量血清鳞状上皮细胞癌抗原1和2的水平,并研究了鳞状上皮细胞癌抗原用于宫颈癌、肺癌和头颈部鳞状上皮细胞癌鉴别诊断的潜能。在欧洲三个研究中心进行了精确度和方法学比较实验。健康人群的参考区间使用来自欧洲和中国人群的样本确定的。鉴别诊断试验确定了鳞状上皮细胞癌抗原水平能否将宫颈癌、肺癌或头颈癌与表观健康的、良性的或其他恶性群组区分开来。根据95%特异性下的鳞状上皮细胞癌抗原水平计算出鳞状上皮细胞癌抗原医学截断值。9个分析浓度的重复性变异系数<5.3%,中间精密变异系数<0.3%。方法学比较显示,与Architect和Kryptor系统具有很好的相关性(斜率分别为1.1和1.5)。表观健康人群的第95百分位数的参考区间为2.3 ng/m L(95%置信区间:1.9-3.8;欧洲队列,n=153)和2.7 ng/m L(95%置信区间:2.2-3.3;中国队列,n=146)。最佳的鉴别诊断结果见于宫颈鳞状上皮细胞癌:受试者工作特征曲线分析显示鳞状上皮细胞癌抗原水平(2.9 ng/m L的医学截断值)鉴别宫颈鳞状上皮细胞癌(n=127)与表观健康女性(n=286;曲线下面积:86.2%;95%置信区间:81.8-90.6;灵敏度:61.4%;特异性:95.6%),良性疾病(n=187;曲线下面积:86.3%;95%置信区间:81.2-91.3;灵敏度:61.4%;特异性:95.0%)和其他宫颈癌(n=157;曲线下面积:78.9%;95%置信区间:70.8-87.1;灵敏度:61.4%;特异性:86.7%)。鳞状上皮细胞癌还可帮助鉴别诊断肺癌。Elecsys鳞状上皮细胞癌检测技术在宫颈鳞状上皮细胞癌的临床实践中表现出了良好的性能,适合用于鉴别诊断。 | Stefan HOLDENRIEDER Rafael MOLINA Ling QIU Xiuyi ZHI Sandra RUTZ Christine ENGEL Pia KASPER- SAUER Farshid DAYYANI Catharina Mkorse | 2018 | 中国肺癌杂志2018，21，7： | 13 |
| 16 | Exercise-induced oxidative stress:Friend or foe?显示文摘The first report demonstrating that prolonged endurance exercise promotes oxidative stress in humans was published more than 4 decades ago.Since this discovery,many ensuing investigations have corroborated the fact that muscular exercise increases the production of reactive oxygen species(ROS)and results in oxidative stress in numerous tissues including blood and skeletal muscles.Although several tissues may contribute to exercise-induced ROS production,it is predicted that muscular contractions stimulate ROS production in active muscle fibers and that skeletal muscle is a primary source of ROS production during exercise.This contraction-induced ROS generation is associated with(1)oxidant damage in several tissues(e.g.,increased protein oxidation and lipid peroxidation),(2)accelerated muscle fatigue,and(3)activation of biochemical signaling pathways that contribute to exercise-induced adaptation in the contracting muscle fibers.While our understanding of exercise and oxidative stress has advanced rapidly during the last decades,questions remain about whether exercise-induced increases in ROS production are beneficial or harmful to health.This review addresses this issue by discussing the site(s)of oxidant production during exercise and detailing the health consequences of exercise-induced ROS production. | Scott K.Powers Rafael Deminice Mustafa Ozdemir Toshinori Yoshihara Matthew P.Bomkamp Hayden Hyatt | 2020 | Journal of Sport and Health Science2020，9，5： | 13 |
| 17 | 精子DNA完整性与形态超高倍检测在辅助生殖技术妊娠结局中的临床价值显示文摘近年来,涌现出许多重要的男性不育诊疗新技术。本研究旨在探讨精@DNA完整性与精子空泡率检测对以男性不育因素行IvF或IcsI治疗的不育症的治疗结局。本研究针对152对不育症夫妻行ART技术前进行的精子DNA碎片率与形态学超高倍分析进行队列研究。我们发现对妊娠结局最有预测价值的DNA碎片率切点值为25.5%,阴性预测值为72.7%(P=0.02)。在对空泡率的研究中发现,3、4级的空泡精子的最有预测价值切点值为73.5%,阴性预测值为39.4%(P=0.09),差异无统计学意义。总之,当精子DNA碎片率大于25%时,IVF失败的概率增大,而精子形态学超高倍检测结果对预测患者的妊娠结局无明显获益。 | Gemma López Rafael Lafuente Miguel A Checa Ramón Carreras Mario Brassesco | 2013 | Asian Journal of Andrology2013，15，6： | 12 |
| 18 | COX-2 in liver,from regeneration to hepatocarcinogenesis:What we have learned from animal models?显示文摘The use of animals lacking genes or expressing genes under the control of cell-specific promoters has signifi cantly increased our knowledge of the genetic and molecular basis of physiopathology,allowing testing of functional hypotheses and validation of biochemical and pharmacologic approaches in order to understand cell function.However,with unexpected frequency,gene knockout animals and,more commonly,animal models of transgenesis give experimental support to even opposite conclusions on gene function.Here we summarize what we learned on the role of cyclooxygenase 2(COX-2) in liver and revise the results obtained in 3 independent models of mice expressing a COX-2 transgene specifi cally in the hepatocyte.Upon challenge with pro-inflammatory stimuli,the animals behave very differently,some transgenic models having a protective effect but others enhancing the injury.In addition,one transgene exerts differential effects on normal liver physiology depending on the transgenic animal model used. | Paloma Martín-Sanz Rafael Mayoral Marta Casado Lisardo Boscá | 2010 | World Journal of Gastroenterology2010，16，12： | 12 |
| 19 | Generation of iPS cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame显示文摘导致的 pluripotent 的产生从体的细胞的茎(iPS ) 细胞被定义 reprogramming 抄写因素(TF ) 的同时的病毒的 transduction 成功地完成了。然而,这个过程为基因交货要求多重病毒的向量。作为结果,产生了 iPS 房间港口在他们的染色体的众多的病毒的集成地点。这能增加基因 mutagenesis 和 genomic 不稳定性的概率,并且介绍重要障碍给研究和 iPS 房间的临床的申请研究。在这篇论文,我们在场定义因素是的一个简单 lentivirus reprogramming 在系统经由自我劈开的 2A 序列熔化了在里面框架进一个单个开的读物框架(ORF ) 。一个 GFP 标记下游地被放 transgene 启用 transgene 表示追踪。我们证明这个 polycistronic 表示系统高效地产生 iPS 房间。产生 iPS 房间有正常 karyotypes 并且类似于鼠标在形态学和基因表示的胚胎的干细胞。而且,他们能在 vitro 并且在 vivo 试金在两个区分进三胚胎的细菌层的房间类型。显著地,大多数这些 iPS 房间仅仅怀有病毒的向量的一个单个拷贝。这个系统提供一个珍贵工具因为 iPS 房间的产生,和我们的数据建议在每个房间的 transduced reprogramming TF 的表示的平衡为 reprogramming 进程是必要的。更重要地,当由非集成的基因交货系统交付了时,这重新设计的单个 ORF 将没有基因修正便于人的 iPS 房间的有效产生。 | Lijian Shao Wei Feng Yan Sun Hao Bai Jun Liu Caroline Currie Jaejung Kim Rafael Gama Zack Wang Zhijian Qian Lucy Liaw Wen-Shu Wu | 2009 | Cell Research2009，19，3： | 12 |
| 20 | New Insights into Aluminum Tolerance in Rice: The ASR5 Protein Binds the STAR1 Promoter and Other Aluminum-Responsive Genes显示文摘在植物的铝(艾尔) 毒性是在庄稼生产的主要限制之一。艾尔 3+, 最艾尔的有毒的形式,在酸的条件和原因下面被释放进土壤植物的广泛的损坏, especiallyin 根。在米饭,艾尔忍耐要求 ASR5 基因,而是 ASR5 的分子的功能仍然保持未知。这里,我们执行染色体宽的分析在米饭识别 ASR5-dependentAl-responsive 基因。在植物把 silencing 基于 ASR5_RNAi,全球 transcriptome 分析识别了全部的 of961 对在野类型的米饭根的艾尔处理应答的基因。这些基因, 909 没在 ASR5_RNAiplants 对艾尔作出回应,显示为在 Al 应答的基因表示的 ASR5 的一个中央角色。在正常条件下面,没有艾尔处理, ASR5_RNAi 植物与野类型的植物相比表示了 1.756 基因差别,并且在野类型的植物的 446 这些基因 respondedto 艾尔处理。深定序跟随的染色质 immunoprecipitation 识别了被对他们的倡导者有约束力的 ASR5 直接调整的 104 putativetarget 基因,包括 STAR1 基因,它编码为艾尔忍耐要求的 ABC transporter。有约束力的山峰序列的主题分析为 ASR5 揭示了 thebinding 主题,它在 vitro 被证实经由用 STAR1 倡导者的 DNA 有约束力的试金。这些结果证明 ASR5 充当在 rice.Key 词为 Al 应答的 geneexpression 和艾尔忍耐是必要的一个关键抄写因素: | Rafael Augusto Arenhart Yang Bai Luiz Felipe Valter de Oliveira Lauro Bucker Neto Mariana Schunemann Felipe dos Santos Maraschin Jorge Mariath Adriano Silverio Gilberto Sachetto-Martins Rogerio Margis Zhi-Yong Wang Marcia Margis-Pinheiro | 2014 | Molecular Plant2014，7，4： | 12 |
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