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| 1 | Comparison of Proliferative and Multilineage Differentiation Potential of Human Mesenchymal Stem Cells Derived from Umbilical Cord and Bone Marrow显示文摘 | DoloresBaksh RaphaelYao Rocky S.Tuan | 2009 | STEM CELLS2009，，6： | 2 |
| 2 | Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy显示文摘 | D.Baksh L.Song R. S.Tuan | 2007 | Journal of Cellular and Molecular Medicine2007，，3： | 2 |
| 3 | Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis显示文摘Tissue (re)vascularization strategies face various challenges, as therapeutic cells do not survive long enough in situ, while the administration of pro-angiogenic factors is hampered by fast clearance and insufficient ability to emulate complex spatiotemporal signaling. Here, we propose to address these limitations by engineering a functional biomaterial capable of capturing and concentrating the pro-angiogenic activities of mesenchymal stem cells (MSCs). In particular, dextran sulfate, a high molecular weight sulfated glucose polymer, supplemented to MSC cul-tures, interacts with MSC-derived extracellular matrix (ECM) components and facilitates their co-assembly and accumulation in the pericellular space. Upon decellularization, the resulting dextran sulfate-ECM hybrid material can be processed into MIcroparticles of SOlidified Secretome (MIPSOS). The insoluble format of MIPSOS protects protein components from degradation, while facilitating their sustained release. Proteomic analysis demonstrates that MIPSOS are highly enriched in pro-angiogenic factors, resulting in an enhanced pro-angiogenic bioactivity when compared to naïve MSC-derived ECM (cECM). Consequently, intravital microscopy of full-thickness skin wounds treated with MIPSOS demonstrates accelerated revascularization and healing, far superior to the ther-apeutic potential of cECM. Hence, the microparticle-based solidified stem cell secretome provides a promising platform to address major limitations of current therapeutic angiogenesis approaches. | Thomas Spater Marisa Assunçao Kwok Keung Lit Guidong Gong Xiaoling Wang Yi-Yun Chen Ying Rao Yucong Li Chi Him Kendrick Yiu Matthias W.Laschke Michael D.Menger Dan Wang Rocky S.Tuan Kay-Hooi Khoo Michael Raghunath Junling Guo Anna Blocki | 2022 | Bioactive Materials2022，7，11： | 1 |
| 4 | Identification and Functional Analysis of Candidate Genes Regulating Mesenchymal Stem Cell Self‐Renewal and Multipotency显示文摘 | LinSong Nicole E.Webb YingjieSong Rocky S.Tuan | 2009 | STEM CELLS2009，，7： | 1 |
| 5 | Cross‐talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation显示文摘 | DoloresBaksh Genevieve M.Boland Rocky S.Tuan | 2007 | J Cell Biochem2007，，5： | 1 |
| 6 | Regulation of stemness and stem cell niche of mesenchymal stem cells: Implications in tumorigenesis and metastasis显示文摘 | Nastaran Z.Kuhn Rocky S.Tuan | 2009 | J. Cell. Physiol2009，，2： | 1 |
| 7 | Comparison of Proliferative and Multilineage Differentiation Potential of Human Mesenchymal Stem Cells Derived from Umbilical Cord and Bone Marrow显示文摘 | DoloresBaksh RaphaelYao Rocky S.Tuan | 2009 | STEM CELLS2009，，6： | 1 |
| 8 | Particle bioreactivity and wear-mediated osteolysis显示文摘 | Mark L.Wang Peter F.Sharkey Rocky S.Tuan | | 0，，08： | 1 |
| 9 | Regulation of stemness and stem cell niche of mesenchymal stem cells: Implications in tumorigenesis and metastasis显示文摘 | Nastaran Z.Kuhn Rocky S.Tuan | 2009 | J Cell Physiol2009，，2： | 1 |
| 10 | Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy显示文摘 | D.Baksh L.Song R. S.Tuan | 2007 | Journal of Cellular and Molecular Medicine2007，，3： | 1 |
| 11 | “Slow walk”mimetic tensile loading maintains human meniscus tissue resident progenitor cells homeostasis in photocrosslinked gelatin hydrogel显示文摘Meniscus,the cushion in knee joint,is a load-bearing tissue that transfers mechanical forces to extracellular matrix(ECM)and tissue resident cells.The mechanoresponse of human tissue resident stem/progenitor cells in meniscus(hMeSPCs)is significant to tissue homeostasis and regeneration but is not well understood.This study reports that a mild cyclic tensile loading regimen of~1800 loads/day on hMeSPCs seeded in 3-dimensional(3D)photocrosslinked gelatin methacryloyl(GelMA)hydrogel is critical in maintaining cellular homeostasis.Experimentally,a“slow walk”biomimetic cyclic loading regimen(10%tensile strain,0.5 Hz,1 h/day,up to 15 days)is applied to hMeSPCs encapsulated in GelMA hydrogel with a magnetic force-controlled loading actuator.The loading significantly increases cell differentiation and fibrocartilage-like ECM deposition without affecting cell viability.Transcriptomic analysis reveals 332 mechanoresponsive genes,clustered into cell senescence,mechanical sensitivity,and ECM dynamics,associated with interleukins,integrins,and collagens/matrix metalloproteinase pathways.The cell-GelMA constructs show active ECM remodeling,traced using a green fluorescence tagged(GFT)-GelMA hydrogel.Loading enhances nascent pericellular matrix production by the encapsulated hMeSPCs,which gradually compensates for the hydrogel loss in the cultures.These findings demonstrate the strong tissue-forming ability of hMeSPCs,and the importance of mechanical factors in maintaining meniscus homeostasis. | Jing Sun Yau Tsz Chan Ki Wai Kevin Ho Li Zhang Liming Bian Rocky S.Tuan Yangzi Jiang | 2023 | Bioactive Materials2023，，7： | 0 |
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