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| 1 | Sulfolobus tokodaii RadA paralog,stRadC2,is involved in DNA recombination via interaction with RadA and Hjc显示文摘Rad51/RadA paralogs found in eukaryotes and euryarchaea play important roles during recombination and repair,and mutations in one of the human Rad51 paralogs,Rad51C,are associated with breast and ovarian cancers.The hyperthermophilic crenarchaeon Sulfolobus tokodaii encodes four putative RadA paralogs and studies on these proteins may assist in understanding the functions of human Rad51 paralogs.Here,we report the biochemical characterization of stRadC2,a S.tokodaii RadA paralog.Pull-down assays revealed that the protein was able to interact with the recombinase,RadA,and the Holliday junction endonuclease,Hjc.stRadC2 inhibited the strand exchange activity of RadA and facilitated Hjc-mediated Holliday junction DNA cleavage in vitro.RT-PCR analysis revealed that stRadC2 transcription was immediately reduced after UV irradiation,but was restored to normal levels at the late stages of DNA repair.Our results suggest that stRadC2 may act as an anti-recombination factor in DNA recombinational repair in S.tokodaii. | WANG Lei SHENG DuoHong HAN WenYuan HUANG Bin ZHU ShanShan NI JinFeng LI Jia SHEN YuLong | 2012 | Science China(Life Sciences)2012，55，3： | 3 |
| 2 | Chemiluminescence assay for reactive oxygen species scavenging activities and inhibition on oxidative damage of DNA in Deinococcus radiodurans显示文摘 | Tian Bing Wu Yuanyuan Sheng Duohong | 2004 | Luminescence2004，19，2： | 1 |
| 3 | Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli显示文摘 | Guanjun Gao Bing Tian Lili Liu Duohong Sheng Binghui Shen Yuejin Hua | 2003 | DNA Repair2003，，12： | 1 |
| 4 | Expression of Deinococcus radiodurans PprI enhances the radioresistance of Escherichia coli显示文摘 | Guanjun Gao Bing Tian Lili Liu Duohong Sheng Binghui Shen Yuejin Hua | 2003 | DNA Repair2003，，12： | 1 |
| 5 | Co-expression with RadA and the characterization of stRad55B, a RadA paralog from the hyperthermophilic crenarchaea Sulfolobus tokodaii显示文摘ST0838 (designed stRad55B) is one of the four RadA paralogs (or Rad55 homologues) in the genome of the hyperthermophilic crenarchaeon Sulfolobus tokodaii. The gene is induced by UV irradiation, sug-gesting that it is involved in DNA recombinational repair in this organism. However, this protein could not be expressed normally in vitro. In this study, thermostable and soluble stRad55B was obtained by co-expression with S. tokodaii RadA (stRadA) in E. coli, and the enzymatic properties were examined. It was found that stRad55B bound ssDNA preferentially and had a very weak ATPase activity that was not stimulated by DNA. The recombinant protein inhibited the strand exchange activity promoted by stRadA, indicating that stRad55B might be an inhibitor to the homologous recombination in this ar-chaeon. The results will be helpful for further functional and interaction analysis of RadA paralogs and for the understanding of the mechanism of recombinational repair in archaea. | SHENG DuoHong, LI MingFeng, JIAO JianDong, NI JinFeng & SHEN YuLong State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China | 2008 | Science China(Life Sciences)2008，51，1： | 0 |
| 6 | Genetic manipulation and tools in myxobacteria for the exploitation of secondary metabolism显示文摘Myxobacteria are famous for their capacity for social behavior and natural product biosynthesis.The unique sociality of myxobacteria is not only an intriguing scientific topic but also the main limiting factor for their ma-nipulation.After more than half a century of research,a series of genetic techniques for myxobacteria have been developed,rendering these mysterious bacteria manipulable.Here,we review the advances in genetic manipu-lation of myxobacteria,with a particular focus on the exploitation of secondary metabolism.We emphasize the necessity and urgency of constructing the myxobacterial chassis for synthetic biology research and the exploita-tion of untapped secondary metabolism. | Xinjing Yue Duohong Sheng Li Zhuo Yue-Zhong Li | 2023 | Engineering Microbiology2023，3，2： | 0 |
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