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| 1 | IGHG1 promotes motility likely through epithelial-mesenchymal transition in ovarian cancer显示文摘Objective: Ovarian cancer(OC) is one of the leading causes of death for female cancer patients. COC166-9 is an OC-specific monoclonal antibody and we have identified immunoglobulin γ-1 heavy chain constant region(IGHG1) as its antigen. We explore the function of IGHG1 in proliferation, apoptosis and motility of OC cells further in this research.Methods: IGHG1 expression in OC specimens was detected through immunohistochemistry. Real-time quantitative polymerase chain reaction(RT-q PCR) or western blotting assay was used to test IGHG1 expression in OC cells. Viability of OC cells was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay. Flow cytometry or western blotting assay was used to detect cell cycle and apoptosis. Cellular motility was analyzed by using transwell assay and the markers of epithelial-mesenchymal transition(EMT) were tested through immunoblots.Results: Although it exerts negligible effect on the viability and apoptosis of OC cells, IGHG1 could promote migration and invasion of malignant cells in vitro. Mechanistically, IGHG1 increases the expression of N-cadherin and Vimentin while decreases E-cadherin expression. Additionally, IGHG1 expression in OC specimens is higher relative to the paired normal counterparts. Further analysis demonstrates that the increased IGHG1 expression correlates positively with the lymph node metastasis of OC.Conclusions: IGHG1 promotes the motility of OC cells likely through executing the EMT program. Increased IGHG1 expression in OC specimens is associated with the lymph node metastasis. | Jingfeng Qian Fangxing Ji Xue Ye Hongyan Cheng Ruiqiong Ma Xiaohong Chang Chengchao Shou Heng Cui | 2018 | Chinese Journal of Cancer Research2018，30，2： | 6 |
| 2 | High level of serum AMBP is associated with poor response to paclitaxel–capecitabine chemotherapy in advanced gastric cancer patients显示文摘 | Hao Huang Yong Han Jing Gao Junnan Feng Lei Zhu Like Qu Lin Shen Chengchao Shou | 2013 | Medical Oncology2013，，4： | 3 |
| 3 | Protein P37 of mycoplasma hyorhinis induces secretion of TNF-αfrom human peripheral blood mononuclear cells显示文摘High mycoplasmal infection ratio in gastric cancer tissues suggests a possible association between my-coplasma infection and tumorigenesis. Because TNF-a plays an important role in carcinogenesis caused by microbes in-fection and P37 is a major immunogen of mycoplasma hy-orhinis (M. hyor.), investigating whether P37 could induceexpression and secretion of TNF-a will be very significant to elucidate the possible molecular mechanism of gastric car-cinogenesis involved with M. hyor. At the present study, we cloned full gene of p37 by PCR and mutated the 7 codes of TGA into TGG firstly, then expressed the P37 protein suc-cessfully with pGEX-4T-1 vector in E. coli, which was veri-fied with Western blot. By RT-PCR and sensitive L929 cell toxic assay, we found that P37 protein could induce expres-sion and secretion of TNF-a from human peripheral bloodmononuclear cells, and the inducing activity of P37 could be dramatically blocked by McAb PD4. These results suggestthat the induction of TNF-a secretion by P37 probably plays an important role in diseases caused by M. hyor. infectionand needs to be further investigated. | NING Jinying, HUANG Su, WU Jian, MENG Lin & SHOU Chengchao Department of Biochemistry and Molecular Biochemistry, School ofOncology, Peking University and Beijing Institute for Cancer Research, Beijing 100034, China | 2003 | Chinese Science Bulletin2003，48，7： | 3 |
| 4 | Expression of VEGF receptor KDR in different originated carcinomas显示文摘 | Song Shumei Wujian Shou Chengchao | 1998 | Chin J Cancer Res1998，10，3： | 1 |
| 5 | The association of the expression level of protein tyrosine phosphatase PRL-3 protein with liver metastasis and prognosis of patients with colorectal cancer显示文摘 | Lirong Peng Jinying Ning Ling Meng Chengchao Shou | 2004 | Journal of Cancer Research and Clinical Oncology2004，，9： | 1 |
| 6 | The effect of antibody against vascular endothelial growth factor on tumor growth and metastasis显示文摘 | Guiqui Wang Zhiwei Dong Guangwei Xu Zhihua Yang Chengchao Shou Naiqin Wang Tong Liu | 1998 | Journal of Cancer Research and Clinical Oncology1998，，11： | 1 |
| 7 | A peptide fusion protein inhibits angiogenesis and tumor growth by blocking VEGF binding to KDR显示文摘Vascular endothelial growth factor (VEGF) binding to its tyrosine kinase receptors (KDR/FLK1, Flt-1) induces angiogenesis. In search of the peptides blocking VEGF binding to its receptor KDR/FLK1 to inhibit tumor-angiogenesis and growth, we screened a phage display pep-tide library with KDR as target protein, and some candidate peptides were isolated. In this study, we cloned the DNA fragment coding the peptide K237 from the library, into a vector pQE42 to express fusion protein DHFR-K237 in E. coli M15. The affection of fusion protein DHFR-K237 on endothelial cell proliferation and angiogenesis was investigated. In vitro, DHFR-K237 could completely block VEGF binding to KDR and significantly inhibit the VEGF-medi-ated proliferation of the human vascular endothelial cells. In vivo, DHFR-K237 inhibited angiogenesis in chick embryo chorioa- llantoric membrane and tumor growth in nude mice. These results suggest that K237 is an effective antagonist of VEGF binding to KDR, and could be a potential agent | LEI Hetian SHOU Chengchao LIU Xiaoyin HE Luowen WU Jian JIANG Beihai LIU Meisheng YANG Junshan | 2002 | Chinese Science Bulletin2002，47，8： | 1 |
| 8 | The association of the expression level of protein tyrosine phosphatase PRL-3 protein with liver metastasis and prognosis of patients with colorectal cancer显示文摘 | Lirong Peng Jinying Ning Ling Meng Chengchao Shou | 2004 | Journal of Cancer Research and Clinical Oncology2004，，9： | 1 |
| 9 | Cancer and embryo expression protein 65 promotes cancer cell growthand metastasis显示文摘 | Genglin Jin Lirong Peng Jianzhi Zhang Like Qu Chengchao Shou | 2015 | Oncology Letters2015，，4： | 1 |
| 10 | The effect of antibody against vascular endothelial growth factor on tumor growth and metastasis显示文摘 | Guiqui Wang Zhiwei Dong Guangwei Xu Zhihua Yang Chengchao Shou Naiqin Wang Tong Liu | 1998 | Journal of Cancer Research and Clinical Oncology1998，，11： | 1 |
| 11 | Aiphanol, a native compound, suppresses angiogenesis via dual-targeting VEGFR2 and C0X2显示文摘Dear Editor,Pathological neo-vascularization is a hallmark of cancer and several diseases.Accumulating evidence supports the notion that antiangiogenic treatment can abolish tumor angiogenesis to achieve Ion ger disease-free survival.Although growth factors and their receptors function as the main drivers in angiogenesis,the involvement of other regulators,e.g.,Cyclooxygenase-2(COX2),^(1) should also be con sidered,especially for managi ng the resista nee to therapies against receptor tyrosine kinases(RTKs). | Shanmei Chen Junnan Feng Chuanke Zhao Lixin Wang Lin Meng Caiyun Liu Shaoqing Cai Yanxing Jia Like Qu Chengchao Shou | 2022 | Signal Transduction and Targeted Therapy2022，7，1： | 0 |
| 12 | Correction: Aiphanol, a native compound, suppresses angiogenesis via dual-targeting VEGFR2 and COX2显示文摘Correction to:Signal Transduction and Targeted Therapy http://gffzzd3cc09b8251d45dfsok59k60fxxuw6von.ffgz.tsg.suse.edu.cn/10.1038/s41392-021-00739-5,published online 03 December 2021 After online publication of the letter1,the author found two images in the supplement materials were used incorrectly,Additionally,there is an error in the chemical structure of Aiphanol in Figs.1a and 1s that needs to be corrected.The correct data are provided as follows.The key findings of the article are not affected by these corrections.The original article has been corrected. | Shanmei Chen Junnan Feng Chuanke Zhao Lixin Wang Lin Meng Caiyun Liu Shaoqing Cai Yanxing Jia Like Qu Chengchao Shou | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 0 |
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