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| 1 | Characterization and Evaluation of OsLCT1 and OsN ramp5 Mutants Generated Through CRISPR/Cas9-Mediated Mutagenesis for Breeding Low Cd Rice显示文摘To explore how rice(Oryza sativa L.) can be safely produced in Cd-polluted soil, OsLCT1 and OsNramp5 mutant lines were generated by CRISPR/Cas9-mediated mutagenesis. One of OsLCT1 mutant(lct1×1) and two of OsNramp5 mutants(nramp5×7 and nramp5×9) were evaluated for grain Cd accumulation and agronomic performances. In paddy field soil containing approximately 0.9 mg/kg Cd, lct1×1 grains contained approximately 40%(0.17 mg/kg) of the Cd concentration of the wild type parental line, less than the China National Food Safety Standard(0.20 mg/kg). Both OsNramp5 mutants showed low grain Cd accumulation(< 0.06 mg/kg) in the paddy(approximately 0.9 mg/kg Cd) or in pots in soil spiked with 2 mg/kg Cd. However, only nramp5×7 showed normal growth and yield, whereas the growth of nramp5×9 was severely impaired. The study showed that lct1×1 could be used to produce rice grains safe for human consumption in lightly contaminated paddy soils and nramp5×7 used in soils contaminated by much higher levels of Cd. | LIU Songmei JIANG Jie LIU Yang MENG Jun XU Shouling TAN Yuanyuan LI Youfa SHU Qingyao HUANG Jianzhong | 2019 | Rice science2019，26，2： | 15 |
| 2 | Fine mapping and candidate gene analysis of purple pericarp gene Pb in rice (Oryza sativa L.)显示文摘Purple rice is a type of rice with anthocyanins deposited in its grain pericarp. The rice Pb gene control-ling purple pericarp character is known to be on chromosome 4,and the purple color is dominant over white color. In this study,we fine mapped the Pb gene using two F2 segregating populations,i.e. Pei'ai 64S(white) × Yunanheixiannuo(purple) and Pei'ai 64S × Chuanheinuo(purple) . In the first-pass map-ping,the Pb gene was located in the region downstream the SSR marker RM3820. In the fine mapping,the candidate region was saturated with InDel and CAPS markers developed specifically for this study. Eventually,the Pb gene was mapped within the 25-kb region delimited by the upstream marker RID3 and the downstream marker RID4. The delimited region contained two annotated genes,Ra and bhlh16(TIGR Rice Genome,R.5) . The former is a homologue of the Myc transcription factor Lc controlling anthocyanin biosynthesis in maize,and the latter is a homologue of the TT8 gene,which is also an Myc transcription factor gene controlling the pericarp pigmentation in Arabidopsis thaliana. Sequence analysis showed that the exon 7 of the Ra gene of Yunanheixiannuo and Chuanheinuo had a 2-bp(GT) deletion compared with those of the white rice varieties Pei'ai 64S,9311 and Nipponbare. A CAPS marker,CAPSRa,was developed according to the GT deletion for analysis of the two F2 segregating populations and 106 rice lines. The results showed that all F2 plants with white pericarp,and all non-purple rice lines(63 white and 22 red) contained no GT deletion,but all 20 purple rice lines con-tained the GT deletion. These results suggested that the Ra gene may be the Pb gene and the purple pericarp characteristic of rice is caused by the GT deletion within exon 7 of the Ra gene. | WANG Caixia SHU Qingyao | 2007 | Chinese Science Bulletin2007，52，22： | 12 |
| 3 | Rice Aroma: A Natural Gift Comes with Price and the Way Forward显示文摘Aromatic rice belongs to a small but important sub-group of rice,which is highly regarded for its excellent aroma and superior grain quality.Aromatic rice,especially Basmati-and Jasmine-type rice,is being traded at a high price in the local and global markets.Genetically,rice aroma is a phenotypical expression of spontaneous recessive mutations of the OsBadh2 gene(also known as fgr/badh2/osbadh2/os2AP gene).These mutations inhibit the flow ofγ-aminobutyraldehyde(GAB-ald)toγ-aminobutyric acid(GABA),and consequently,the accumulated GAB-ald is diverted to a potent flavour component 2-acetyl-1-pyrroline(2AP)by a non-enzymatic reaction with methylglyoxal.The natural incidence of non-functional osbadh2 mutation along with selection and nursing by the farmer from the ancient time makes rice aroma as a prominent natural gift.As GABA and methylglyoxal play significant roles in stress tolerance,and their biosynthesis is strictly regulated in rice plants,the accumulation of 2AP in aromatic rice depends on the interaction of various genetic and environmental factors,and its production may come at some costs of sacrificing tolerance.This review focused on some potential underlying genes in the 2AP and GABA biosynthesis pathways,and analyzed most aspects of aroma formation in rice,and summarized the molecular mechanism of aroma production together with its genetic and non-genetic influencing factors.The present review also stated approaches to produce high-quality aromatic rice via developing novel cultivars and with good agronomic knowledge-based practice. | Zakaria Hossain PRODHAN SHU Qingyao | 2020 | Rice science2020，27，2： | 8 |
| 4 | Transgenic rice plants with a synthetic cry1Ab gene from Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species显示文摘 | Qingyao Shu Gongyin Ye Hairui Cui Xiongying Cheng Youbin Xiang Dianxing Wu Mingwei Gao Yingwu Xia Cui Hu Ravinder Sardana Illimar Altosaar | 2000 | Molecular Breeding2000，，4： | 2 |
| 5 | Identification of a major quantitative trait locus and its candidate underlying genetic variation for rice stigma exsertion rate显示文摘Stigma exsertion in male sterile lines of hybrid rice is important for seed yield.In the present study, ZS616 [Oryza sativa subsp.Xian(indica)], a male sterile line with a stigma exsertion rate(SER) as high as 94.5%, was crossed to DS552, a japonica line with almost no exserted stigmas.F3 plants with extremely low and high SER were sequenced to identify SER-associated quantitative trait loci(QTL).A major QTL for SER, qSER-3.1, was identified along with other QTL on chromosome 3 in a 3.9 Mb region.A total of 307 nonsynonymous single-nucleotide polymorphisms(SNPs) and 27 frame-shift insertion/deletions(InDels)differentiating ZS616 and DS552 were identified in the region containing qSER-3.1.Most SNPs(294) and InDels(25) were excluded after further analysis because they were shared by ZS616 and low(<2.0%) SER accessions in the Huazhong Agricultural University(HAU) core rice collection.Association analysis using the full HAU collection identified a 17-bp InDel in OS03 G0689400 as the most likely causal genetic variant underlying qSER-3.1.ZS616-type accessions(n = 54, with the 17-bp insertion) in the HAU collection had minimum(16.5%)and mean(39.6%) SERs significantly greater than those(n = 424) without the insertion(with minimum and mean SERs of 0.2% and 20.6%, respectively).Thus, this study identified a major QTL for stigma exsertion and revealed the mutation in a candidate gene for the QTL. | Shouling Xu Yunchao Zheng Yang Liu Xiaohao Guo Yuanyuan Tan Qiuping Qian Qingyao Shu Jianzhong Huang | 2019 | The Crop Journal2019，7，3： | 2 |
| 6 | Nuclear translocation of OsMFT1 that is impeded by OsFTIP1 promotes drought toleranee in rice显示文摘Drought is the leading environmental threat affecting crop productivity,and plants have evolved a series of mechanisms to adapt to drought stress.The FT-interacting proteins(FTIPs)and phosphatidylethanola mine.binding proteins(PEBPs)play key roles in developmental processes,whereas their roles in the regulation of stress response are still largely unknown.Here,we report that OsFTIP1 negatively regulates drought response in rice.We showed that OsFTIP1 interacts with rice MOTHER OF FT AND TFL1(OsMFT1),a PEBP that promotes rice tolerance to drought treatment.Further studies discovered that OsMFT1 interacts with two key drought-related transcription factors,OsbZIP66 and OsMYB26,regulating their binding capacity on drought-related genes and thereby enhancing drought toleranee in rice.Interestingly,we found that OsFTIP1 impedes the nucleocytoplasmic translocation of OsMFT1,implying that dynamic modulation of drought-responsive genes by the OsMFT1-OsMYB26 and OsMFT1-OsbZIP66 complexes is integral to OsFTIP1-modulated nuclear accumulation of OsMFT1.Our findings also suggest that OsMFT1 might act as a hitherto unknown nucleocytoplasmic trafficking signal that regulates drought tolerance in rice in response to environmental signals. | Ying Chen Jun Shen Liang Zhangi Haoyue Qi Lijia Yang Huanyu Wang Jiaxuan Wang Yuexing Wang Hao Du Zeng Tao Ting Zhao Pingchuan Deng Qingyao Shu Qian Qian Hao Yu Shiyong Song | 2021 | Molecular Plant2021，14，8： | 2 |
| 7 | Effect of gamma irradiation on starch viscosity and Physicochemical properties of different rice 显示文摘 | Dianxing Wu Qingyao Shu Zhonghua Wang Yingwu Xia | 2002 | Radiation Physics and Chemistry2002，65，： | 1 |
| 8 | Effect of gam- ma irradiation on starch viscosity and physicochemical properties of different rice显示文摘 | Dianxing Wu Qingyao Shu Zhonghua Wang | 2002 | Radiation Physics and Chemistry2002，，65： | 1 |
| 9 | Transgenic rice plants with a synthetic cry1Ab gene from Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species显示文摘 | Qingyao Shu Gongyin Ye Hairui Cui Xiongying Cheng Youbin Xiang Dianxing Wu Mingwei Gao Yingwu Xia Cui Hu Ravinder Sardana Illimar Altosaar | 2000 | Molecular Breeding2000，，4： | 1 |
| 10 | Effect of gamma irradiation on starch viscosity and physicochemical properties of different rice显示文摘 | Dianxing Wu Qingyao Shu Zhonghua Wang | 2002 | Radiation Physics and Chemistry2002，65，1： | 1 |
| 11 | Transgenic rice plants with a synthetic cry1Ab gene from Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species显示文摘 | Qingyao Shu Gongyin Ye Hairui Cui Xiongying Cheng Youbin Xiang Dianxing Wu Mingwei Gao Yingwu Xia Cui Hu Ravinder Sardana Illimar Altosaar | 2000 | Molecular Breeding2000，，4： | 1 |
| 12 | Transgenic rice plants with a synthetic cry1Ab gene from Bacillus thuringiensis were highly resistant to eight lepidopteran rice pest species显示文摘 | Qingyao Shu Gongyin Ye Hairui Cui Xiongying Cheng Youbin Xiang Dianxing Wu Mingwei Gao Yingwu Xia Cui Hu Ravinder Sardana Illimar Altosaar | 2000 | Molecular Breeding2000，，4： | 1 |
| 13 | ^(60)Co irradiation effect on cooked rice texture and amylopectin structure显示文摘The effect of γ irradiation on the cooked rice texture and amylopectin structure is studied with three varieties (Zhefu 504, Zhefu 802 and Zaogeng T2) subjected to 4kGy, skGy and 12kGy irradiation. The results indicate that irradiation has significant effect on the texture of the cooked rice; the hardness, cohesiveness,gumminess and chewiness reduce significantly with the increase of the irradiation dose.Irradiation also affects the chain length distribution of the amylopectin, the side-chains of amylopectin of Zhefu 504 with the 12DP, Zhefu 802 with 24DP13 and Zaogeng T2 with 24DP13 and 36DP25 are broken. | BAO Jinsong SHU Qingyao Christine BERGMAN XIA Yingwu Anna McCLUNG (Institute of Nuclear Agricultural Sciences, Zhejiang University, Huajiachi,Hangzhou, 310029 China USDA/ARS, AT 7, Box 999, Beaumont, TX 77713, USA ) | 1999 | Nuclear Science and Techniques1999，10，3： | 0 |
| 14 | Current insights on rice(Oryza sativa L.)bakanae disease and exploration of its management strategies显示文摘Bakanae is an emerging rice disease caused by the seed-and soil-borne pathogen Fusarium fujikuroi.It is becoming a more serious threat to sustainable rice production throughout rice-growing regions.Bakanae disease infection is responsible for high yield losses ranging from 3%to 95%,and disease incidence varies based on the region and cultivars.Hence,understanding the nature of the pathogen,its pathogenicity,disease epidemiology,symptoms,host–pathogen interaction,and the role of secondary metabolites in the disease cycle will be helpful in the development of effective and sustainable management strategies.However,very few comprehensive studies have described the details of rice bakanae disease.Thus,in this review we summarize and discuss in detail the information available from 1898 to 2023 on various critical facets of bakanae disease,and provide perspectives on future research. | Chinnannan KARTHIK Qingyao SHU | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，9： | 0 |
| 15 | Analysis of variation in temperature-responsive leaf color mutant lines induced from Gamma irradiation in rice(Oryza sativa L.)显示文摘Eight lines of temperature responsive leat color mutants induced by applying 300 Gy Gammaray irradiation to Thermo-sensitive genic male sterile line 2177s, were obtained through continuous selection in seven generations. The leaves of these lines started to become green after the fourth leaf extension, and except LCM 6, all lines recovered to normal green at the sixth leaf age. | WU Dianxing SHU Qingyao XIA Yingwu LIU Guifu Inst of Nuclear Agri Sci,Zhejiang Agri Univ,Hangzhou 310029,China | 1997 | Chinese Rice Research Newsletter1997，5，2： | 0 |
| 16 | Melatonin activates the OsbZIP79–OsABI5 module that orchestrates nitrogen and ROS homeostasis to alleviate nitrogen-limitation stress in rice显示文摘Melatonin(Mel)has previously been reported to effectively alleviate nitrogen-limitation(N-L)stress and thus increase nitrogen-use efficiency(NUE)in several plants,but the underlying mechanism remains obscure.Here,we revealed that OsbZIP79(BASIC LEUCINE ZIPPER 79)is transcriptionally activated under N-L conditions,and its expression is further enhanced by exogenous Mel.By the combined use of omics,genetics,and biological techniques,we revealed that the OsbZIP79–OsABI5(ABSCISIC ACID INSENSITIVE 5)module stimulated regulation of reactive oxygen species(ROS)homeostasis and the uptake and metabolismof nitrogen under conditions of indoor nitrogen limitation(1/16 normal level).OsbZIP79 activated the transcription of OsABI5,and OsABI5 then bound to the promoters of target genes,including genes involved in ROS homeostasis and nitrogen metabolism,activating their transcription.This module was also indispensable for upregulation of several other genes involved in abscisic acid catabolism,nitrogen uptake,and assimilation under N-L and Mel treatment,although these genes were not directly transactivated by OsABI5.Field experiments demonstrated that Mel significantly improved rice growth under low nitrogen(L-N,half the normal level)by the same mechanism revealed in the nitrogen-limitation study.Mel application produced a 28.6%yield increase under L-N and thus similar increases in NUE.Also,two OsbZIP79-overexpression lines grown in L-N field plots had significantly higher NUE(+13.7%and+21.2%)than their wild types.Together,our data show that an OsbZIP79–OsABI5 module regulates the rice response to N insufficiency(N limitation or low N),which is important for increasing NUE in rice production. | Meng Jiang Yue Song Ruifang Yang Chenfan Zheng Yunchao Zheng Huali Zhang Shan Li Yuanyuan Tan Jianzhong Huang Qingyao Shu Ruiqing Li | 2023 | Plant Communications2023，4，6： | 0 |
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