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| 1 | Reference quality assembly of the 3.5-Gb genome of Capsicum annuum from a single linked-read library显示文摘Linked-Read sequencing technology has recently been employed successfully for de novo assembly of human genomes,however,the utility of this technology for complex plant genomes is unproven.We evaluated the technology for this purpose by sequencing the 3.5-gigabase(Gb)diploid pepper(Capsicum annuum)genome with a single Linked-Read library.Plant genomes,including pepper,are characterized by long,highly similar repetitive sequences.Accordingly,significant effort is used to ensure that the sequenced plant is highly homozygous and the resulting assembly is a haploid consensus.With a phased assembly approach,we targeted a heterozygous F1 derived from a wide cross to assess the ability to derive both haplotypes and characterize a pungency gene with a large insertion/deletion.The Supernova software generated a highly ordered,more contiguous sequence assembly than all currently available C.annuum reference genomes.Over 83%of the final assembly was anchored and oriented using four publicly available de novo linkage maps.A comparison of the annotation of conserved eukaryotic genes indicated the completeness of assembly.The validity of the phased assembly is further demonstrated with the complete recovery of both 2.5-Kb insertion/deletion haplotypes of the PUN1 locus in the F1 sample that represents pungent and nonpungent peppers,as well as nearly full recovery of the BUSCO2 gene set within each of the two haplotypes.The most contiguous pepper genome assembly to date has been generated which demonstrates that Linked-Read library technology provides a tool to de novo assemble complex highly repetitive heterozygous plant genomes.This technology can provide an opportunity to cost-effectively develop high-quality genome assemblies for other complex plants and compare structural and gene differences through accurate haplotype reconstruction. | Amanda M.Hulse-Kemp Shamoni Maheshwari Kevin Stoffel Theresa A.Hill David Jaffe Stephen R.Williams Neil Weisenfeld Srividya Ramakrishnan Vijay Kumar Preyas Shah Michael C.Schatz Deanna M.Church Allen Van Deynze | 2018 | Horticulture Research2018，5，1： | 4 |
| 2 | 水中痕量含氯除草剂的在线富集及自动样品净化的简单快速方法显示文摘在线富集分析了酸化水中的8种含氯除草剂:毒莠定,草灭平,麦草畏,灭草松,2,4-D,滴丙酸,2,4,5,-T,P三氟羧草醚.这些除草剂可以在少于2 mL的水中进行分析,运行时间在15 min以内.使用Agilent ZORBAX SB-Aq色谱柱用于捕获分析物,Agilent ZORBAX SB-C18色谱柱用于分析,一个两位六通阀用于色谱柱切换.通过毒莠定和滴丙酸的标准曲线,从而验证了水中这些农药定量方法的系统适应性.自动样品净化省去了样品制备时繁杂的固相萃取(SPE)程序.Agilent 1260 Infinity LC系统的灵敏度可以达到EPA(美国环境保护署)方法的检出限要求,而且只需使用少于EPA官方方法所要求的样品量的十分之一. | Srividya Kailasam | 2013 | 环境化学2013，32，3： | 1 |
| 3 | Stress and protective factors in individuals at ultra-high risk for psychosis,first episode psychosis and healthy controls显示文摘 | Pruessner M Srividya Nr Faridi K | 2011 | Schizophrenia Research2011，129，1： | 1 |
| 4 | Sustained ophthalmicdelibery of ofloxacin from a pH triggered in situ gelling system显示文摘 | Srividya B Cardoza RM Amin PD | 2001 | J Control Release2001，73，2： | 1 |
| 5 | Homozy- gous 10q23/PTEN deletion and its impact on outcome in glioblastoma: a prospective translational study on a uniformly treated cohort of adult patients显示文摘 | Srividya MR Thota B Shailaja BC | 2011 | Neu- ropathology2011，31，4： | 1 |
| 6 | Impact of a FACTS controller on reliability of composite power genera- tion and transmission system显示文摘 | Verma Ajit Kumar Srividya A Deka Bimal C | 2004 | Electric Power Systems Research2004，72，2： | 1 |
| 7 | Automated Diagnosis of Rolling Element Bearing Defects Using Time domain Features and Neural Networks显示文摘 | Srividya A Verma A K Sreejith B | 2009 | Internation- al Journal of Mining Reclamation and Environment2009，23，3： | 1 |
| 8 | Automated diagnosis of rolling element bearing defects using time-domain features and neural networks显示文摘 | Srividya A Verma A K Sreejith B | 2009 | International Journal of Mining Reclamation and Environment2009，23，3： | 1 |
| 9 | Sustained ophthalmic delivery of ofloxacin from a pH triggered in situ gelling system显示文摘 | Srividya B Cardoza RM Amin PD | 2001 | J Controlled Release2001，73，2： | 1 |
| 10 | Sustained ophthalmic delivery of ofloxacin from a pH triggered in situ gelling system 显示文摘 | Srividya B Cardoza RM Amin PD | 2001 | J Controlled Release2001，73，2： | 1 |
| 11 | Sustained ophthalmic delivery of ofloxacin from a pH triggered in situ gelling system显示文摘 | Srividya B Cardoza RM Amin PD | 2001 | J Control Rel2001，73，2： | 1 |
| 12 | Titanium bufferlayer for improved field emission of CNT based cold cathode显示文摘 | SRIVIDYA S GAUTAM S jHA P | 2010 | Applied Surface Science2010，256，35： | 1 |
| 13 | Ring opening of cyclic anydrides: Synthesis of achiral half - esters using lewis acids 显示文摘 | SABITHA G SRIVIDYA R YADAY J S | 1999 | Tetrahedron1999，55，： | 1 |
| 14 | Responsetime Modeling of Controller Area Network(CAN)显示文摘 | KUAMR M VRMAR K A SRIVIDYA A | | 0，，： | 1 |
| 15 | Surface and CorrosionCharacteristics of a-C: H/Fluorocarbon Films显示文摘 | Srividya C Babu S V Visser S A | 1998 | J Adhesion1998，67，： | 1 |
| 16 | Assciation of adrenal steroids with hypertension and the metabolic syndrome in blacks 显示文摘 | Srividya K Jane M Clarence E | 2007 | Hypertension2007，49，： | 1 |
| 17 | Folding of a universal ribozyme: the ribonuclease P RNA显示文摘 | Baird NJ Fang XW Srividya N | 2007 | Q Rev Biophys2007，40，2： | 1 |
| 18 | Fuzzy dynamic reliability evaluation of a deteriorating system under imperfect repair 显示文摘 | VERMA A K SRIVIDYA A RAJESH P G | 2004 | International Journal of Reliability Quality and Safety Engineering2004，11，： | 1 |
| 19 | Impact of a FACTS controller on reliability of composite power generation and transmission system 显示文摘 | Verma A K Srividya A Deka B C | 2004 | Electric Power Systems Research (S0378-7796)2004，72，2： | 1 |
| 20 | Sustained ophthalmic delivery of ofloxacin from a pH triggered in situ gelling system 显示文摘 | SRIVIDYA B CARDOZA R M AMIN P D | 2001 | J Contr Release2001，73，2： | 1 |
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