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| 1 | NAT2*6A, a haplotype of the N-acetyltransferase 2 gene, is an important biomarker for risk of anti-tuberculosis drug-induced hepatotoxicity in Japanese patients with tuberculosis显示文摘AIM: To investigate an association between N -acetyltransferase 2 (NAT2 )-haplotypes/diplotypes and adverse effects in Japanese pulmonary tuberculosis patients. METHODS: We studied 100 patients with pulmonary TB treated with anti-TB drugs including INH. The frequencies and distributions of single nucleotide polymorphisms, haplotypes, and diplotypes of NAT2 were determined by the PCR-restriction fragment length polymorphism method, and the results were compared between TB patients with and without adverse effect, using multivariate logistic regression analysis.RESULTS: Statistical analysis revealed that the frequency of a variant haplotype, NAT2*6A , was signifi cantly increased in TB patients with hepatotoxicity, compared with those without hepatotoxicity [P = 0.001, odds ratio (OR) = 3.535]. By contrast, the frequency of a wild-type (major) haplotype, 'NAT2*4', was signif icantly lower in TB patients with hepatotoxicity than those without hepatotoxicity (P < 0.001, OR = 0.265). There was no association between NAT2-haplotypes and skin rash or eosinophilia. CONCLUSION: The present study shows that NAT2 is one of the determinants of anti-TB drug-induced hepatotoxicity. Moreover, the haplotypes, NAT2*4 and NAT2*6A, are useful new biomarkers for predicting anti- TB drug-induced hepatotoxicity. | Norihide Higuchi Naoko Tahara Katsunori Yanagihara Kiyoyasu Fukushima Naofumi Suyama Yuichi Inoue Yoshitsugu Miyazaki Tsutomu Kobayashi Koh-ichiro Yoshiura Norio Niikawa, Chun-Yang Wen, Hajime Isomoto,Saburou Shikuwa, Katsuhisa Omagari, Yohei Mizuta, Shigeru Kohno, Kazuhiro Tsukamoto Norio Niikawa Chun-Yang Wen Hajime Isomoto Saburou Shikuwa Katsuhisa Omagari Yohei Mizuta Shigeru Kohno Kazuhiro Tsukamoto | 2007 | World Journal of Gastroenterology2007，13，45： | 21 |
| 2 | Diagnosis of mild chronic pancreatitis (Cambridge classification):Comparative study using secretin injection-magnetic resonance cholangiopancreatography and endoscopic retrograde pancreatography显示文摘AIM:To investigate the usefulness of secretin injection-MRCP for the diagnosis of mild chronic pancreatitis. METHODS:Sixteen patients having mild chronic pancreatitis according to the Cambridge classification and 12 control subjects with no abnormal findings on the pancreatogram were examined for the diagnostic accuracy of secretin injection-MRCP regarding abnormal branch pancreatic ducts associated with mild chronic pancreatitis (Cambridge Classification), using endoscopic retrograde cholangiopancreatography (ERCP) for comparison. RESULTS:The sensitivity and specificity for abnormal branch pancreatic ducts determined by two reviewers were respectively 55%-63% and 75%-83% in the head, 57%-64% and 82%-83% in the body, and 44%-44% and 72%-76% in the tail of the pancreas. The sensitivity and specificity for mild chronic pancreatitis were 56%-63% and 92%-92%, respectively. Interobserver agreement (κ statistics) concerning the diagnosis of an abnormal branch pancreatic duct and of mild chronic pancreatitis was good to excellent. CONCLUSION:Secretin injection-MRCP might be useful for the diagnosis of mild chronic pancreatitis. | Masafumi Suyama Yoshihiro Kubokawa Sumio Watanabe | 2008 | World Journal of Gastroenterology2008，14，8： | 11 |
| 3 | A case of gallbladder carcinoma associated with pancreatobiliary reflux in the absence of a pancreaticobiliary maljunction:A hint for early diagnosis of gallbladder carcinoma显示文摘有胆囊墙的进步变厚的一个 62 岁的人访问了我们的门诊病人诊所。在胆总管的胆汁的淀粉酶水平是 19,900 IU/L,胆囊的是 127,000 IU/L,尽管内视镜后退 cholangiopancreatography 没揭示 pancreaticobiliary maljunction。组织学表明了胆囊的中等区分的腺癌。Pancreatobiliary 倒流和联系胆囊癌在现在的盒子中被证实,当 pancreaticobiliary maljunction 不在时。pancreatobiliary 倒流并且胆囊墙的进步变厚的更早的察觉可能导致了胆囊的更早的切除术并且改进了这个病人的差的预后。 | Masafumi Suyama Yoshihiro Kubokawa | 2006 | World Journal of Gastroenterology2006，12，28： | 6 |
| 4 | Two distinct pathways of p16 gene inactivation in gallbladder cancer显示文摘AIM: To examine the mechanism of inactivation of the p16 gene in gallbladder cancer,and to investigate p16 alterations and their correlation with clinicopathological features. METHODS: Specimens were collected surgically from 51 patients with gallbladder cancer. We evaluated the status of protein expression,loss of heterozygosity (LOH),homozygous deletion and promoter hypermethylation using immunohistochemistry,microsatellite analysis,quantitative real-time polymerase chain reaction (PCR) and methylation-specific PCR,respectively. In addition,mutations were examined by direct DNA sequencing. RESULTS: Homozygous deletions of the p16 gene exon2,LOH at 9p21-22,p16 promoter hypermethylation,and loss of p16 protein expression were detected in 26.0% (13/50),56.9% (29/51),72.5% (37/51) and 62.7% (32/51),respectively. No mutations were found. LOH at 9p21 correlated with the loss of p16 protein expression (P < 0.05). Homozygous deletion of the p16 gene,a combination LOH and promoter hypermethylation,and multiple LOH at 9p21 were significantly correlated with the loss of p16 protein expression (P < 0.05). LOH at 9p21 and promoter hypermethylation of the p16 gene were detected in 15.4% (2/13) and 92.3% (12/13) of the tumors with homozygous deletion of the p16 gene,respectively. P16 alterations were not associated with clinicopathological features. CONCLUSION: Our results suggest that LOH and homozygous deletion may be two distinct pathways in the inactivation of the p16 gene. Homozygous deletion,a combination of LOH and promoter hypermethylation,and multiple LOH are major mechanisms of p16 inactivation in gallbladder cancer. | Hiroyuki Tadokoro Takako Shigihara Tomomi Ikeda Masaru Takase Masafumi Suyama | 2007 | World Journal of Gastroenterology2007，13，47： | 6 |
| 5 | Gallbladder carcinoma associated with pancreatobiliary reflux显示文摘AIM: To detect the patients with and without pan-creaticobiliary maljunction who had pancreatobiliary reflux with extremely high biliary amylase levels.METHODS: Ninety-six patients, who had diffuse thickness (> 3 mm) of the gallbladder wall and were suspected of having a pancreaticobiliary maljunction on ultrasonography, were prospectively subjected to endoscopic retrograde cholangiopancreatography, and bile in the common bile duct was sampled. Among them, patients, who had extremely high biliary amylase levels (>10 000 IU/L), underwent cholecystectomy, and the clinicopathological findings of those patients with and without pancreaticobiliary maljunction were examined.RESULTS: Seventeen patients had biliary amylase levels in the common bile duct above 10 000 IU/L, including 11 with pancreaticobiliary maljunction and 6 without pancreaticobiliary maljunction. The occurrence of gallbladder carcinoma was 45.5% (5/11) in patients with pancreaticobiliary maljunction, and 50% (3/6) in those without pancreaticobiliary maljunction.CONCLUSION: Pancreatobiliary reflux with extremely high biliary amylase levels and associated gallbladder carcinoma could be identified in patients with and without pancreaticobiliary maljunction, and those patients might be detected by ultrasonography and bile sampling. | Jin Kan Sai Masafumi Suyama Yoshihiro Kubokawa Bunsei Nobukawa | 2006 | World Journal of Gastroenterology2006，12，40： | 5 |
| 6 | Interplay of neuropilin-1 and semaphorin 3A after partial hepatectomy in rats显示文摘AIM:To elucidate the role of neuropilin-1(Nrp-1) and semaphorin 3A(Sema3A) in sinusoidal remodeling during liver regeneration in rats.METHODS:Male Wistar/ST rats at 7 wk of age,weighing about 200 g,were used for all animal experiments.In vivo,at 24,48,72,96,144 and 192 h after twothirds partial hepatectomy(PHx),the remnant livers were removed.Liver tissues were immunohistochemically stained for Nrp-1,Sema3A and SE-1,a liver sinusoidal endothelial cell(SEC) marker.Total RNA of the liver tissue was extracted and reversely transcribed into cDNA.The mRNA expression of Sema3A was analyzed by quantitative real-time polymerase chain reaction and normalized to that of ribosomal protein S18.In vitro,SECs were isolated from rat liver and cultured in endothelial growth medium containing 20 ng/mL vascular endothelial cell growth factor.Migration of SECs in primary culture was assessed by cell transwell assay with or without recombinant Sema3A.Apoptotic cells were determined by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling method.RESULTS:In vitro,immunohistochemistry study revealed that Sema3A and Nrp-1 were constitutively expressed in hepatocytes and SECs,respectively,in normal rat liver tissues.Nrp-1 expression in SECs was quantified by the percentage of immunostained area with antiNrp-1 antibody in relation to the area stained with SE-1.Between 24 h and 96 h following resection of liver,Nrp-1 expression in SECs was transiently increased.Compared with the baseline(5.2% ± 0.1%),Nrp-1 expression in SECs significantly increased at 24 h(17.3% ± 0.7%,P < 0.05),48 h(39.1% ± 0.6%,P < 0.01),72 h(46.9% ± 4.5%,P < 0.01) and 96 h(29.9% ± 3.8%,P < 0.01) after PHx,then returned to the basal level at termination of liver regeneration.Interestingly,the expression of Sema3A was inversely associated with that of Nrp-1 in liver after PHx.Sema3A mRNA expression was significantly reduced by about 75% over the period 24-144 h after PHx(P < 0.05),and returned to basal levels at 192 h after PHx.In vitro,SECs isolated from rats after PHx(PHx-SECs) were observed to migrate to the lower chamber of the cell transwell system after incubation for 24 h,but not cells from normal rats(CONT-SECs),indicating that mobility of PHx-SECs increases as compared with that of CONT-SECs.Moreover,recombinant Sema3A significantly attenuated migration in PHx-SECs in primary culture(vehicle-treated 100% ± 7.9% vs Sema3A-treated 42.6% ± 5.4%,P < 0.01),but not in CONT-SECs.Compared with CONTSECs,the apoptotic rate of PHx-SECs decreased by 78.3%(P < 0.05).There was no difference in apoptosis between CONT-SECs that were treated with vehicle and Sema3A.However,in PHx-SECs,apoptosis was induced by the presence of 5 nmol Sema3A for 24 h(vehicle-treated 21.7% ± 7.6% vs Sema3A-treated 104.3% ± 8.9%,P < 0.05).In addition,immunohistochemistry confirmed the increased expression of Nrp-1 in PHx-SECs,while it was noted to a lesser extent in CONT-SECs.CONCLUSION:The interplay of Nrp-1 and Sema3A shown in our results may lead to a better understanding of interaction between sinusoidal remodeling and SECs during liver regeneration. | Ling Fu Tsuneo Kitamura Kazuhisa Iwabuchi Syozo Ichinose Mitsuaki Yanagida Hideoki Ogawa Sumio Watanabe Toshihide Maruyama Masafumi Suyama Kenji Takamori | 2012 | World Journal of Gastroenterology2012，18，36： | 5 |
| 7 | 利用油橄榄果汁制备低盐固态发酵酱油的初步研究显示文摘对比探究了不同比例油橄榄果汁替代发酵用水制备低盐固态发酵酱油的感官与理化性能。研究显示:以80%的油橄榄果汁替代量进行低盐固态发酵,得到的酱油在感官上与普通酱油相近,并且总酸含量为0.73g/dL,氨基酸态氮含量达到0.55g/dL,全氮含量达到1.20g/dL,氨基酸生成率为45.83%。结果表明:利用一定比例的油橄榄果汁进行低盐固态发酵酿造酱油,在感官和理化指标上都可以达到甚至优于普通低盐固态发酵酱油,研究结果为压榨橄榄油副产物——油橄榄果汁的有效利用提供了新的途径。 | 程述敏 李莉 赵盈盈 吴正云 Suyama Taikei 张文学 | 2019 | 中国调味品2019，44，4： | 4 |
| 8 | Leptin transiently antagonizes ghrelin and long-lastingly orexin in regulation of Ca^(2+) signaling in neuropeptide Y neurons of the arcuate nucleus显示文摘AIM: To explore the mechanism for interactions of leptin with ghrelin and orexin in the arcuate nucleus (ARC) activating neuropeptide Y (NPY) neurons during physiological regulation of feeding. METHODS: Single neurons from ARC of adult rats with matured feeding function were isolated. [Ca2+]i was measured to monitore their activities. The time course of leptin effects on ghrelin-induced versus orexin-induced [Ca2+]i increases in NPY neurons was studied. RESULTS: Administration of ghrelin or orexin-A at 10-10 mol/L increased cytosolic Ca2+ concentration ([Ca2+]i) in NPY neurons isolated from the ARC of adult rats. Upon administration of leptin at 10-14-10-12 mol/L, ghrelin-induced [Ca2+]i increases were initially (< 10 min) inhibited but later restored, exhibiting a transient pattern of inhibition. In contrast, orexin-induced [Ca2+]i increases were inhibited by leptin in a longlasting manner. Furthermore, a prior administration of leptin inhibited orexin action but not ghrelin action to increase [Ca2+]i. CONCLUSION: Leptin counteracted ghrelin effects transiently and orexin effects long-lastingly in NPY neurons. The transient property with which leptin counteracts ghrelin action in NPY neurons may allow the fasting-associated increase in ghrelin levels to activate NPY neurons in the presence of physiological leptin and to stimulate feeding. | Daisuke Kohno Shigetomo Suyama Toshihiko Yada | 2008 | World Journal of Gastroenterology2008，14，41： | 4 |
| 9 | Randomized phase II study of gemcitabine and S-1 combination versus gemcitabine alone in the treatment of unresectable advanced pancreatic cancer (Japan Clinical Cancer Research Organization PC-01 study)显示文摘 | Masato Ozaka Yuji Matsumura Hiroshi Ishii Yasushi Omuro Takao Itoi Hisatsugu Mouri Keiji Hanada Yasutoshi Kimura Iruru Maetani Yoshinobu Okabe Masaji Tani Takaaki Ikeda Susumu Hijioka Ryouhei Watanabe Shinya Ohoka Yuki Hirose Masafumi Suyama Naoto Egawa A | 2012 | Cancer Chemotherapy and Pharmacology2012，，5： | 3 |
| 10 | Serum SP-D levels as a biomarker of lung injury in respiratory syncytial virus bronchiolitis显示文摘 | Kawasaki Y Endo K Suyama K | 2011 | Pediatr Pulmonol2011，46，1： | 1 |
| 11 | Methylprednisolone pulse plus mizoribine in children with Henoch-Schoenlein purpura nephritis显示文摘 | Kawasaki Y Suyama K Hashimoto K | | 0，，04： | 1 |
| 12 | Serum SP-D levels as a biomarker of lung injury in respiratory syncytial virus bronchiolitis显示文摘 | Yukihiko Kawasaki Kisei Endo Kazuhide Suyama | | 0，，01： | 1 |
| 13 | Catheter Ablation of Stable and Unstable Ventricular Tachycardia in Patients with Arrhythmogenic Right Ventricular Dysplasia显示文摘 | Satomi K Kurita T Suyama K | 2006 | J Cardiovasc Electrophysiol2006，5，17： | 1 |
| 14 | Intraglomerular deposition of intact cross-linked fibrin in IgA nephropathy and Henoch-Schonlein purpura nephritis显示文摘 | Ono T Muso E Suyama K | 1996 | Nephron1996，74，3： | 1 |
| 15 | Bone marrow subpopulations contain distinct types of endothelial progenitor cells and angiogenic cvtokine-producing cells显示文摘 | Mjyamoto Y Suyama T Yashita T | 2007 | J Mol Cell Cardiol2007，43，： | 1 |
| 16 | Identification of genes involved in cell invasion by using a library of randomized hybrid ribozymes显示文摘 | SUYAMA E KAWASAKI H NAKAJIMA M | 2003 | Proc Natl Acad Sci USA2003，100，10： | 1 |
| 17 | siRNAs generated by recombinant human Dicer induce specific and significant but target siteindependent gene silencing in human cells显示文摘 | Kawasaki H Suyama E Iyo M | 2003 | Nucleic Acids Res2003，31，： | 1 |
| 18 | Plasma amino acid pat terns in hepatocellular carcinoma显示文摘 | Hirayama C Suyama K Horie Y | 1987 | Biochem Med Metab Biol1987，38，2： | 1 |
| 19 | Cloning cDNAs for Genes Preferentially Expressed During Fruit Growth in Cucumber 显示文摘 | SUYAMA T YAMADA K MORI H | 1999 | J AmSoc HorticSci1999，124，1： | 1 |
| 20 | Localization of Optimal Ablation Site of Idiopathic Ventricular Tachycardia from Right and Left Ventricular Outflow Tract by Body Surface ECG显示文摘 | Shiro Kamakura Wataru Shimizu Kiyotaka Matsuo Atsushi Taguchi Kazuhiro Suyama Takashi Kurita Naohiko Aihara Tohru Ohe Katsuro Shimomura | 1998 | Circulation1998，，15： | 1 |
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