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| 1 | Endoscopic ultrasonography-guided celiac plexus neurolysis in patients with unresectable pancreatic cancer: An update显示文摘Pancreatic cancer produces disabling abdominal pain,and the pain medical management for pancreatic cancer is often challenging because it mainly relies on the use of narcotics(major opioids).However,opioids often provide suboptimal pain relief,and the use of opioids can lead to patient tolerance and several side effects that considerably reduce the quality of life of pancreatic cancer patients.Endosonography-guided celiac plexus neurolysis(EUS-CPN)is an alternative for pain control in patients with nonsurgical pancreatic cancer;EUS-CPN consists of the injection of alcohol and a local anesthetic into the area of the celiac plexus to achieve chemical ablation of the nerve tissue.EUS-CPN via the transgastric approach is a safer and more accessible technique than the percutaneous approach.We have reviewed most of the studies that evaluate the efficacy of EUSCPN and that have compared the different approaches that have been performed by endosonographers.The efficacy of EUS-CPN varies from 50%to 94%in the different studies,and EUS-CPN has a pain relief duration of 4–8 wk.Several factors are involved in its efficacy,such as the onset of pain,previous use of chemotherapy,presence of metastatic disease,EUS-CPN technique,type of needle or neurolytic agent used,etc.According to this review,injection into the ganglia may be the best technique,and a good visualization of the ganglia is the best predictor for a good EUS-CPN response,although more studies are needed.However,any of the 4 different techniques could be used to perform EUS-CPN effectively with no differences in terms of complications between the techniques,but more studies are needed.The effect of EUS-CPN on pain improvement,patient survival and patient quality of life should be evaluated in well-designed randomized clinical trials.Further research also needs to be performed to clarify the best time frame in performing a EUS-CPN. | Guillermo Pérez-Aguado Diego Martinez-Acitores de la Mata Carlos Marra-López Valenciano Ignacio Fernandez-Urien Sainz | 2021 | World Journal of Gastrointestinal Endoscopy2021，13，10： | 4 |
| 2 | 烧结条件与组成对含Cr粉末冶金钢力学性能的影响显示文摘对先进铁基粉末冶金材料的期望,已导致开发了更接近模拟锻钢组成的合金。像Cr等传统锻钢合金化元素,已用于提高力学性能与淬透性,而且成本可行。过去在粉末冶金中避免用铬是因为与氧相关的问题,但现在较新的合金系都含有这种元素。尽管这些合金可提供良好的力学性能与生产总成本可行,但这些合金的适当烧结仍是关键性问题。将介绍烧结条件与组成对含Cr粉末冶金钢的力学性能与显微结构的影响。 | Francisco Castro Shandra Sainz Bruce Lindsley W.Brian James 亓家钟 | 2011 | 粉末冶金技术2011，29，4： | 3 |
| 3 | Potential Use of a Poultry Manure Digestate as a Biofertiliser:Evaluation of Soil Properties and Lactuca sativa Growth显示文摘Anaerobic digestion is a process that is widely used for the treatment of organic wastes. The digestate can be used as a soil amendment or crop fertiliser. The aims of our work were to evaluate 1) the physicochemical composition and pathogen content in a digestate from poultry manure, according to international regulations, and 2) the effect of its soil application on the major chemical and biological soil properties and on the growth of Lactuca sativa. The experiment consisted of two groups of pots(with and without crop). Treatments applied to each group were as follows: low and high doses of digestate and inorganic fertiliser, and no application(control)(low dose: 70 kg nitrogen(N) ha^(-1) and 21 kg phosphorus(P) ha^(-1); high dose: 210 kg N ha^(-1) and 63 kg P ha^(-1)). Soil samples were taken 7 and 34 d(harvest) after treatment applications. Heavy metal and pathogen contents in the digestate were below the upper limit values. Despite the high pH and electrical conductivity values of the digestate, both soil parameters presented acceptable values for crop growth. Although there were no initial increases in total inorganic N and available P in soil with digestate application, an increase in the fresh weight of crop was observed with the high dose application. This is probably associated with the slow nutrient release from the digestate during the development of the crop. Changes in the microbial community were temporary and occurred at the initial sampling stage of the experiment. | Natalia MóRTOLA Romina ROMANIUK Vanina COSENTINO Maximiliano EIZA Patricia CARFAGNO Pedro RIZZO Patricia BRES Nicolás RIERA Marcos ROBA Mariano BUTTI Daiana SAINZ Lucrecia BRUTTI | 2019 | Pedosphere2019，29，1： | 2 |
| 4 | The system Clinker–MgO–CaZrO 3 and its application to the corrosion behavior of CaZrO 3 /MgO refractory matrix by clinker显示文摘 | S. Serena M.A. Sainz A. Caballero | 2009 | Journal of the European Ceramic Society2009，，11： | 2 |
| 5 | Glutathione metabolism is essential for self-renewal and chemoresistance of pancreatic cancer stem cells显示文摘BACKGROUND Cellular metabolism regulates stemness in health and disease.A reduced redox state is essential for self-renewal of normal and cancer stem cells(CSCs).However,while stem cells rely on glycolysis,different CSCs,including pancreatic CSCs,favor mitochondrial metabolism as their dominant energy-producing pathway.This suggests that powerful antioxidant networks must be in place to detoxify mitochondrial reactive oxygen species(ROS)and maintain stemness in oxidative CSCs.Since glutathione metabolism is critical for normal stem cell function and CSCs from breast,liver and gastric cancer show increased glutathione content,we hypothesized that pancreatic CSCs also rely on this pathway for ROS detoxification.AIM To investigate the role of glutathione metabolism in pancreatic CSCs.METHODS Primary pancreatic cancer cells of patient-derived xenografts(PDXs)were cultured in adherent or CSC-enriching sphere conditions to determine the role of glutathione metabolism in stemness.Real-time polymerase chain reaction(PCR)was used to validate RNAseq results involving glutathione metabolism genes in adherent vs spheres,as well as the expression of pluripotency-related genes following treatment.Public TCGA and GTEx RNAseq data from pancreatic cancer vs normal tissue samples were analyzed using the webserver GEPIA2.The glutathione-sensitive fluorescent probe monochlorobimane was used to determine glutathione content by fluorimetry or flow cytometry.Pharmacological inhibitors of glutathione synthesis and recycling[buthionine-sulfoximine(BSO)and 6-Aminonicotinamide(6-AN),respectively]were used to investigate the impact of glutathione depletion on CSC-enriched cultures.Staining with propidium iodide(cell cycle),Annexin-V(apoptosis)and CD133(CSC content)were determined by flow cytometry.Self-renewal was assessed by sphere formation assay and response to gemcitabine treatment was used as a readout for chemoresistance.RESULTS Analysis of our previously published RNAseq dataset E-MTAB-3808 revealed upregulation of genes involved in the KEGG(Kyoto Encyclopedia of Genes and Genomes)Pathway Glutathione Metabolism in CSC-enriched cultures compared to their differentiated counterparts.Consistently,in pancreatic cancer patient samples the expression of most of these up-regulated genes positively correlated with a stemness signature defined by NANOG,KLF4,SOX2 and OCT4 expression(P<10-5).Moreover,3 of the upregulated genes(MGST1,GPX8,GCCT)were associated with reduced disease-free survival in patients[Hazard ratio(HR)2.2-2.5;P=0.03-0.0054],suggesting a critical role for this pathway in pancreatic cancer progression.CSC-enriched sphere cultures also showed increased expression of different glutathione metabolism-related genes,as well as enhanced glutathione content in its reduced form(GSH).Glutathione depletion with BSO induced cell cycle arrest and apoptosis in spheres,and diminished the expression of stemness genes.Moreover,treatment with either BSO or the glutathione recycling inhibitor 6-AN inhibited self-renewal and the expression of the CSC marker CD133.GSH content in spheres positively correlated with intrinsic resistance to gemcitabine treatment in different PDXs r=0.96,P=5.8×1011).Additionally,CD133+cells accumulated GSH in response to gemcitabine,which was abrogated by BSO treatment(P<0.05).Combined treatment with BSO and gemcitabine-induced apoptosis in CD133+cells to levels comparable to CD133-cells and significantly diminished self-renewal(P<0.05),suggesting that chemoresistance of CSCs is partially dependent on GSH metabolism.CONCLUSION Our data suggest that pancreatic CSCs depend on glutathione metabolism.Pharmacological targeting of this pathway showed that high GSH content is essential to maintain CSC functionality in terms of self-renewal and chemoresistance. | Petra Jagust Sonia Alcala Bruno Sainz Jr Christopher Heeschen Patricia Sancho | 2020 | World Journal of Stem Cells2020，12，11： | 2 |
| 6 | Alpha/Beta interferon and gamma interferon synergize to inhibit the replication of herpes simplex virus type I 显示文摘 | Sainz B Jr Halford W P | 2002 | J Virol2002，76，11： | 1 |
| 7 | Modal intervals 显示文摘 | Erenst Gardenes Miguel A Sainz Lambert Jorba | 2001 | Reliable Computing2001，7，2： | 1 |
| 8 | Absolute reliability of five clinical tests for assessing hamstring flexibility in professional futsal players显示文摘 | Francisco Ayala Pilar Sainz de Baranda Mark De Ste Croix Fernando Santonja | 2011 | Journal of Science and Medicine in Sport2011，，2： | 1 |
| 9 | Fault detection and isolation of the three-tank system using the modal interval analysis显示文摘 | Miguel A Sainz Joaquim Armengol Josep Vehf | 2002 | Journal of Process Control2002，，12： | 1 |
| 10 | Isolation of 'side population' progenitor cells from healthy arteries of adult mice显示文摘 | SAINZ J AL HAJ ZEN A CALIGIURI G | 2006 | Arterioscler Thromb Vasc Biol2006，26，2： | 1 |
| 11 | Point-based rendering techniques 显示文摘 | Sainz M Pajarola R | 2004 | Computers & Graphics2004，28，： | 1 |
| 12 | Modeling of yeast metabolism and process dynamics in batch fermentation显示文摘 | SAINZ J PIZARRO F PE′ REZ-CORREA J R | 2003 | Biotechnology and Bioengineering2003，7，81： | 1 |
| 13 | Effect of unilateral and bilateral eyestalk ablation in Litopenaeus vannamei male and female on several metabolic and immunologic variables 显示文摘 | Sainz - Herna' Ndez J C Racotta I S Dumas S | 2008 | Aqua- culture2008，283，1234： | 1 |
| 14 | Melatonin and Parkinson's disease显示文摘 | Mayo JC Sainz RM Tan DX | 2005 | Endocrine2005，27，2： | 1 |
| 15 | The inhibitory effect of HKa in endothelial cell tube formation is mediated by disrupting the uPA- uPAR complex and inhibiting its signaling and internalization 显示文摘 | Liu Y Cao DJ Sainz IM | 2008 | Am J Physiol Cell Physiol2008，295，1： | 1 |
| 16 | Fault detection and isolation of the three-tank system using the modal interval analysis显示文摘 | SAINZ M (A) JOAQUIM A JOSEP V | 2002 | Journal of Process Control2002，，12： | 1 |
| 17 | Nitrogen balance as affected by application time and nitrogen fertilizer rate in irrigated no tillage maize显示文摘 | Sainz H R Barbie A | 2004 | Agron J2004，96，6： | 1 |
| 18 | Cross-Layer Clus- ter-Based Energy-Efficient Protocol for Wireless Sensor Networks 显示文摘 | Mammu A S Hernandez-Jayo U Sainz N | 2015 | Sensors2015，15，4： | 1 |
| 19 | Ultrastructural confirmation of neuronal protection by melatonin against the neurotoxin 6-hydroxydopamine cell damage显示文摘 | Mayo JC Sainz RM Antolin I | 1999 | Brain Res1999，818，2： | 1 |
| 20 | Melatonin regulates glucocorticoid receptor:ananswer to its antiapoptotic action in thymus显示文摘 | Sainz RM Mayo JC Reiter RJ | 1999 | FASEB J1999，13，12： | 1 |
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