|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Coordinated peak expression of MMP-26 and TIMP-4 in preinvasive human prostate tumor显示文摘因为早察觉和治疗为病人的医药管理是批评的,为早前列腺癌症诊断的新奇简历标记的鉴定是高度重要的。在基础房间层和地下室膜的连续性的混乱为高级职业人员静电干扰 intraepithelial 瘤形成(HGPIN ) 的前进是必要的到在人的前列腺的侵略腺癌。涉及变换到侵略显型的分子是强烈审查的题目。我们以前报导了矩阵 metalloproteinase-26 (MMP-26 ) 经由地下室膜蛋白质并且由激活 MMP-9 的酶原形式的劈开支持人的前列腺癌症房间的侵略。而且,我们发现了 metalloproteinases-4 (TIMP-4 ) 的那个织物禁止者是大多数有势力 MMP-26 的内长的禁止者。这里,我们更高示威(p<0.0001 ) 在 HGPIN 和癌症的 MMP-26 和 TIMP-4 表示,与非肿瘤的 acini 相比。他们的表示层次在 HGPIN 是最高的,但是在一样的纸巾在侵略癌症(为各个的 p<0.001 ) 衰退。连续前列腺癌症织物节染色的 Immunohistochemical 建议 MMP-26 和 TIMP-4 的 colocalization。现在的学习显示 MMP-26 和 TIMP-4 可以在 HGPIN 的变换期间起一个不可分的作用到侵略癌症并且可以也为早前列腺癌症诊断用作标记。房间研究(2006 ) 16:750-758。做 i:10.1038/sj .cr.7310089;出版联机 2006 年 8 月 29 日。 | Seakwoo Lee Kevin K Desai Kenneth A Iczkowski Robert G Newcomer Kevin J WU Yun-Ge Zhao Winston W Tan Mark D Roycik Qing-Xiang Amy Sang | 2006 | Cell Research2006，16，9： | 18 |
| 2 | Capecitabine and cisplatin with or without cetuximab for patients with previously untreated advanced gastric cancer (EXPAND): a randomised, open-label phase 3 trial显示文摘 | Florian Lordick Yoon-Koo Kang Hyun-Cheol Chung Pamela Salman Sang Cheul Oh Gy?rgy Bodoky Galina Kurteva Constantin Volovat Vladimir M Moiseyenko Vera Gorbunova Joon Oh Park Akira Sawaki Ilhan Celik Heiko G?tte Helena Melezínková Markus Moehler | 2013 | Lancet Oncology2013，，6： | 14 |
| 3 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 4 | Application of non-stationary signal characteristics using wavelet packet transformation 显示文摘 | SANG G HYOUN J HAE J | 2008 | Journal of Mechanical Science and Technology2008，22，11： | 1 |
| 5 | A PLC programming environment based on a virtual plant显示文摘 | SANG C CHANG M WANG G | 2008 | Int J Adv Manuf Technol2008，39，1112： | 1 |
| 6 | Municipal landfill leachate induces cytogenetie damage in root tips of Hordettm vulgare显示文摘 | Sang N Li G K Xin X Y | 2006 | Ecotoxicology and environ- mental safety2006，63，3： | 1 |
| 7 | Inhibitory effects of gossypol on corticosteroid 11-hydroxysteroid dehydrogenase from guinea pig kidney:a possible mechanism for causing hypokalemia 显示文摘 | SANG G W LORENZO B REIDENBERG M | 1991 | J Steroid Biochem Mol Biol1991，39，1： | 1 |
| 8 | A functional analysis of mouse models of cardiac disease through metabolic profiling显示文摘 | Jones G L Sang E Goddard C | 2005 | J Biol Chem2005，280，9： | 1 |
| 9 | Cross-species analysis of the mammalian beta-defensin gene family:presence of syntenic gene clusters and preferential expression in the male reproductive tract显示文摘 | Patil AA Ca Y Sang Y Blecha F Zhang G | | 0，，01： | 1 |
| 10 | Stress and strength analysis of concentric reducers consisting of plates 显示文摘 | Sang Z F Widem G E O | 1988 | J pres Ves Tech ASME1988，110，： | 1 |
| 11 | Synthesis of scaly Sn3O4/TiO2 nanobelt heterostructures for enhanced UV- visible light photocatalytic activity显示文摘 | Chen G H Ji S Z Sang Y H | 2015 | Nanoscale2015，7，7： | 1 |
| 12 | Fo-cused ion beam fabrication of submicron gold struc-tures显示文摘 | Blauner P G Butt Yousaf Ro Jae Sang | 1989 | Vac Sci Technol B1989，7，4： | 1 |
| 13 | A new unusual iridoid with inhibition of activator protein-1 (AP-1) from the leaves of Morinda citrifolia L显示文摘 | Sang S He K Liu G | 2001 | Org Lett2001，3，9： | 1 |
| 14 | Molecular mechanism of Nrf2 Activation by Oxidative Stress显示文摘 | Keon W K Seung J L Sang G K | 2005 | Antioxid Redox Signal2005，7，1112： | 1 |
| 15 | Effect of Gd implantation on the structural and magnetic properties of GaN and A1N显示文摘 | Han Sang Youn Hite J Thaler G T | 2006 | Applied Physics Letters2006，88，04： | 1 |
| 16 | Discrete wavelet entropy aided detection of abrupt change: a case study in the Haihe River Basin, China显示文摘 | SANG Y F WANG Z G LI Z L | 2012 | Entropy2012，14，7： | 1 |
| 17 | Review on studies of Eupa- torium adenophorum-An important invasive species in Chi- na显示文摘 | Sun X Y Lu Z H Sang W G | 2004 | J Forestry Res2004，15，4： | 1 |
| 18 | Burst analysis of cylindrical shells显示文摘 | Xue Liping Widera G E O Sang Zhifu | 2008 | Journal of Pressure Vessel Technology2008，130，： | 1 |
| 19 | Effects of environmental factors on germination and emergence of Crofton weed (Eupatorium adenophorum) 显示文摘 | Lu P Sang W G Ma K P | 2006 | Weed Science2006，54，： | 1 |
| 20 | Invasion pattern of Eupatorium adenophorum Spreng in southern China 显示文摘 | Sang W G Zhu L Axmacher J C | 2010 | Biological Invasions2010，12，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员