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| 1 | 在结肠和直肠的癌症的淋巴节点收获: 当前的考虑显示文摘 The prognostic significance of identifying lymph node(LN) metastases following surgical resection for colon and rectal cancer is well recognized and is reflected in accurate staging of the disease.An established body of evidence exists,demonstrating an association between a higher total LN count and improved survival,particularly for node negative colon cancer.In node positive disease,however,the lymph node ratios may represent a better prognostic indicator,although the impact of this on clinical treatment has yet to be universally established.By extension,strategies to increase surgical node harvest and/or laboratory methods to increase LN yield seem logical and might improve cancer staging.However,debate prevails as to whether or not these extrapolations are clinically relevant,particularly when very high LN counts are sought.Current guidelines recommend a minimum of 12 nodes harvested as the standard of care,yet the evidence for such is questionable as it is unclear whether an increasing the LN count results in improved survival.Findings from modern treatments,including down-staging in rectal cancer using pre-operative chemoradiotherapy,paradoxically suggest that lower LN count,or indeed complete absence of LNs,are associated with improved survival;implying that using a specific number of LNs harvested as a measure of surgical quality is not always appropriate.The pursuit of a sufficient LN harvest represents good clinical practice;however,recent evidence shows that the exhaustive searching for very high LN yields may be unnecessary and has little in? uence on modern approaches to treatment. | James R McDonald Andrew G Renehan Sarah T O’Dwyer Najib Y Haboubi | 2012 | World Journal of Gastrointestinal Surgery2012，4，1： | 8 |
| 2 | Local protectionism and regional specialization : evidence from China' s industries 显示文摘 | Bai C E Du Y J Tao Z G Sarah Y T | 1997 | Journal of Comparative Economics1997，，6： | 1 |
| 3 | Tooth Discoloration in Patients With Neonatal Diabetes After Transfer Onto Glibenclamide: A previously unreported side effect显示文摘 | Kumaraguru Janani Flanagan Sarah E Greeley Siri Atma W Nuboer Roos St?y Julie Philipson Louis H Hattersley Andrew T Rubio-Cabezas Oscar | 2009 | Diabetes Care2009，，8： | 1 |
| 4 | Intensity of C-reactive Protein Immuno- histochemical staining of atherosclerotie plaque macrophages and extracellular tissue of patients with angina pectoris undergoing directional coronary atherectomy 显示文摘 | Tzu-Hsien T Sarah C Cheng-Hsu Y | 2007 | Chang Gung Med J2007，30，4： | 1 |
| 5 | Intensity of C-reactive Protein immuno-histochemical staining of atherosclerotic plaque macrophages and extracellular tissue of patients with angina pectoris undergoing directional coronary atherectomy显示文摘 | Tzu-Hsien T Sarah C Cheng-Hsu Y | 2007 | Chang Gung Med J2007，30，4： | 1 |
| 6 | Methicillin-resistant staphylococcus aureus, Hawaii, 2000-2002显示文摘 | Li F Sarah Y P Ayers T L | 2005 | Emerg Infeet Dis2005，11，： | 1 |
| 7 | Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system显示文摘BACKGROUND Mesenchymal stem cells(MSCs)represent a promising therapy for the treatment of equine joint diseases,studied due to their possible immunomodulatory characteristics and regenerative capacity.However,the source of most suitable MSCs for producing cartilage for regenerative processes in conjunction with biomaterials for an enhanced function is yet to be established.AIM To compare the chondrogenicity of MSCs derived from synovial fluid,bone marrow,and adipose tissue of horses,using the aggrecan synthesis.METHODS MSCs from ten horses were cultured,phenotypic characterization was done with antibodies CD90,CD44 and CD34 and were differentiated into chondrocytes.The 3D cell culture system in which biocompatible nanoparticles consisting of gold,iron oxide,and poly-L-lysine were added to the cells,and they were forced by magnets to form one microspheroid.The microspheroids were exposed to a commercial culture medium for 4 d,7 d,14 d,and 21 d.Proteoglycan extraction was performed,and aggrecan was quantified by enzyme-linked immunosorbent assay.Keratan sulfate and aggrecan in the microspheroids were identified and localized by immunofluorescence.RESULTS All cultured cells showed fibroblast-like appearance,the ability to adhere to the plastic surface,and were positive for CD44 and CD90,thus confirming the characteristics and morphology of MSCs.The soluble protein concentrations were higher in the microspheroids derived from adipose tissue.The aggrecan concentration and the ratio of aggrecan to soluble proteins were higher in microspheroids derived from synovial fluid than in those derived from bone marrow,thereby showing chondrogenic superiority.Microspheroids from all sources expressed aggrecan and keratan sulfate when observed using confocal immunofluorescence microscopy.All sources of MSCs can synthesize aggrecan,however,MSCs from synovial fluid and adipose tissue have demonstrated better biocompatibility in a 3D environment,thus suggesting chondrogenic superiority.CONCLUSION All sources of MSCs produce hyaline cartilage;however,the use of synovial liquid or adipose tissue should be recommended when it is intended for use with biomaterials or scaffolds. | Joice Fülber Fernanda R Agreste Sarah R T Seidel Eric D P Sotelo Ângela P Barbosa Yara M Michelacci Raquel Y A Baccarin | 2021 | World Journal of Stem Cells2021，13，6： | 0 |
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