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| 1 | Osteopontin is an important mediator of alcoholic liver disease via hepatic stellate cell activation显示文摘AIM: To investigate over-expression of Osteopontin(OPN) pathway expression and mechanisms of action in human alcoholic liver disease(ALD), in vivo and in vitro acute alcohol models. METHODS: OPN pathway was evaluated in livers from patients with progressive stages of human ALD and serum from drinkers with and without liver cirrhosis. In vitro stellate LX2 cells exposed to acute alcohol and in vivo in acute alcoholic steatosis mouse models were also investigated for OPN pathway expression and function. WT and OPN-/- mice were administered an acute dose of alcohol and extent of liver injury was examined by histopathology and liver biochemistry after 16-24 h. The causative role of OPN was studied in OPN knockout animals and in vitro in stellate LX2 cells, utilizing siRNA, aptamer and neutralizing antibodies to block OPN and OPN pathway. OPN pathway expression and downstream functional consequences were measured for signaling by Western blotting, plasmin activation by spectrophotometric assays and cell migration by confocal imaging and quantitation. RESULTS: OPN expression positively correlated with disease severity in patients with progressive stages of ALD. In vivo, associated with alcoholic steatosis, a single dose of acute alcohol significantly increased hepatic OPN mRNA and protein, and a cleaved OPN form in a dose dependent manner. OPN mRNA and secreted OPN also increased in parallel with activation of LX2 stellate cells within 4 h of a single dose of alcohol. Expression of OPN receptors, αvβ3-integrin and CD44, increased in human ALD, and in vivo and in vitro with alcohol administration. This was accompanied by downstream phosphorylation of Akt and Erk, increased mRNA expression of several fibrogenesis, fibrinolysis and extracellular matrix pathway genes, plasmin activation and hepatic stellate cell(HSC) migration. Inhibition of OPN and OPN-receptor mediated signaling partially inhibited alcohol-induced HSC activation, plasmin activity and cell migration. CONCLUSION: OPN is a key mediator of the alcoholinduced effects on hepatic stellate cell functions and liver fibrogenesis. | Devanshi Seth Alastair Duly Paul C Kuo Geoffrey W McCaughan Paul S Haber | 2014 | World Journal of Gastroenterology2014，20，36： | 5 |
| 2 | Screening for gestational diabetes: a summary of the evidence for the U.S. Preventive Services Task Force显示文摘 | Seth C Brody Russell Harris Kathleen Lohr | 2003 | Obstetrics & Gynecology2003，，2： | 3 |
| 3 | SLC26A4 mutation testing for hearing loss associated with enlargement of the vestibular aqueduct显示文摘Pendred syndrome(PS) is characterized by autosomal recessive inheritance of goiter associated with a defect of iodide organification, hearing loss, enlargement of the vestibular aqueduct(EVA), and mutations of the SLC26A4 gene. However, not all EVA patients have PSor SLC26A4 mutations. Two mutant alleles of SLC26A4 are detected in 1/4 of North American or European EVA populations, one mutant allele is detected in another 1/4 of patient populations, and no mutations are detected in the other 1/2. The presence of two mutant alleles of SLC26A4 is associated with abnormal iodide organification, increased thyroid gland volume, increased severity of hearing loss, and bilateral EVA. The presence of a single mutant allele of SLC26A4 is associated with normal iodide organification, normal thyroid gland volume, less severe hearing loss and either bilateral or unilateral EVA. When other underlying correlations are accounted for, the presence of a cochlear malformation or the size of EVA does not have an effect on hearing thresholds. This is consistent with observations of an Slc26a4 mutant mouse model of EVA in which hearing loss is independent of endolymphatic hydrops or inner ear malformations. Segregation analyses of EVA in families suggest that the patients carrying one mutant allele of SLC26A4 have a second, undetected mutant allele of SLC26A4, and the probability of a sibling having EVA is consistent with its segregation as an autosomal recessive trait. Patients without any mutations are an etiologically heterogeneous group in which siblings have a lower probability of having EVA. SLC26A4 mutation testing can provide prognostic information to guide clinical surveillance and management, as well as the probability of EVA affecting a sibling. | Taku Ito Julie Muskett Parna Chattaraj Byung Yoon Choi Kyu Yup Lee Christopher K Zalewski Kelly A King Xiangming Li Philine Wangemann Thomas Shawker Carmen C Brewer Seth L Alper Andrew J Griffith | 2013 | World Journal of Otorhinolaryngology2013，3，2： | 2 |
| 4 | A techno-economic assessment for viability of some waste as cooling pads in evaporative cooling system显示文摘The viability of some waste as cooling pads for evaporative cooling application in South-Western Nigeria was experimentally assessed.This is to ascertain their effectiveness as a substitute for costly imported pads in a low income environment.Also presented was the feasibility of utilizing standalone evaporative coolers for storage and selling of fruits in South-Western Nigeria.Natural ambient air was forced through the various pads at three different fan speeds and constant cooling pad thickness of 30 mm.Performance characteristics were considered based on daily analysis using temperature and humidity data measured from morning to evening at location co-ordinates latitude 7°10′N and longitude 5°05′E for 6 weeks.The daily temperature T and humidity h ranged between 26℃≤T≤45℃ and 28%≤h_(2)≤80%.Temperature differenceDT and humidity differenceDh of 0.6℃≤ΔT≤18.3℃ and 1.0%≤Δh≤53%was achieved for the four cooling pad materials tested at three fan speeds.HighestDT andDh was recorded at fan speed of 4 m/s with shredded latex foam and jute sack respectively.The cooling efficiency(η)calculated for all the pads under the three speeds ranged from 17.3%≤η≤98.8%.Payback period(PBP)analysis indicated the considered EVC is economically feasible and investors will break even in 1.75 years. | Macmanus C Ndukwu Seth I Manuwa | 2015 | International Journal of Agricultural and Biological Engineering2015，8，2： | 2 |
| 5 | Cardiac resynchronization therapy for the treatment of heart failure in patients with intraventricular conduction delay and malignant ventricular tachyarrhythmias显示文摘 | Steven L Higgins John D Hummel Imran K Niazi Michael C Giudici Seth J Worley Leslie A Saxon John P Boehmer Michael B Higginbotham Teresa De Marco Elyse Foster Patrick G Yong | 2003 | Journal of the American College of Cardiology2003，，8： | 2 |
| 6 | A Tutorial on Visual Servo Control显示文摘 | Seth H Gregory D H Peter I C | 1996 | IEEE Trans on Robotics and Automation1996，12，65： | 1 |
| 7 | Alteration of cerebral neurotransmitter receptor function by exposure of rats to manganese显示文摘 | Seth P Hong J Kits C | 1981 | Toxicol Lett1981，9，3： | 1 |
| 8 | Degradation of some phthalic acid esters in soil显示文摘 | Shanker R Ramakrishma C Seth P K | 1985 | Environmental Pollution Series A Ecological and Biological1985，39，1： | 1 |
| 9 | Damage mechanics approach for bearing lifetime prognostics显示文摘 | Qiu J Zhang C Seth B B Liang S Y | 2002 | Mechanical Systems and Signal Processing2002，16，5： | 1 |
| 10 | Exposure to feline an canine pathogens in obcats an grey foxes in urban an rural zones of a national park in California显示文摘 | SETH P D RILEY J F BRUNO C | 2004 | J Wildl Dis2004，40，1： | 1 |
| 11 | Botulinum toxin-A for the treatment of overactive bladder: UK contributions 显示文摘 | Seth JH Dowson C Khan MS | 2013 | J Clin Urol2013，6，2： | 1 |
| 12 | Efficacy of postoperative patient - controlled and continuous ifusion epidural analgesia versus intravenous patient controlled analgesia with opioi- da 显示文摘 | CHRISTOPHER L W SETH R COHEN B C | 2005 | Anrsthesiology2005，103，6： | 1 |
| 13 | Alteration of cerebral neurotransmitter receptor function by exposure of rats to manganese显示文摘 | SETH P HONG J KITS C | 1981 | Toxicol Lett1981，9，3： | 1 |
| 14 | Why do immunoassays for tumor markers give differing result?-a view from the UK National External Quality Assessment Schemes显示文摘 | Sturgeon C M Seth J | 1996 | Eur J Clim Chem Biochem1996，34，： | 1 |
| 15 | Properties of a-Si:H films deposited from silane diluted with hydrogen and helium using modified pulse plasma technique显示文摘 | Mukherjee C Anandan C Seth T | 1999 | Journal of Vacuum Science & Technology A:Vacuum Surfaces and Films1999，17，6： | 1 |
| 16 | The effect of grammage of sheet properties显示文摘 | Seth R S Hanturen J T Moss C S | 1989 | Appita Journal1989，42，1： | 1 |
| 17 | Spherical mosaics with quaternions and dense cor relation显示文摘 | Sayan C Seth T | 2000 | International Journal of Computer Vision2000，37，3： | 1 |
| 18 | Matrix metalloproteinases in disease and repair progresses in the anterior segment显示文摘 | Wong TT Seth C Daniels JT | 2002 | Suru Opthalmal2002，47，3： | 1 |
| 19 | Photoconducting a-Si: H films grown at high deposition by pulsing a VHF100MHz discharge显示文摘 | Mukherjee C Anandan C Seth T | 2003 | Thin Solid Films2003，423，1： | 1 |
| 20 | AmiGO:online access to ontology and annotation data显示文摘 | Seth C Amelia I Christopher J | 2009 | Bioinformatics2009，25，： | 1 |
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