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| 1 | A scoring model for predicting the prognosis of severe viral hepatitis显示文摘The prognosis of patients with severe viral hepatitis is concerned by clinicians, patients and their relatives. Many factors may influence on the prognosis of patients with this disease. Many studies on the prognosis of severe viral hepatitis by multiple logistic regression analysis have shown generally consistent results.1-4 Previouly we established a scoring model of severe viral hepatitis (SMSVH) by logistic regression analysis.1 The aim of this study was to estimate prospectively the 6-month survival rate of patients with severe viral hepatitis using SMSVH. | DINGHui-guo XIANGHai-ping SHANJing ZHOULi MABing LIUMin WANGJun-tao | 2005 | Chinese Medical Journal2005，，3： | 13 |
| 2 | Efficient decolorization of dye-containing wastewater using mycelial pellets formed of marine-derived Aspergillus niger显示文摘In order to improve the efficient decolorization of dye-containing water by biosorbent and understand the biosorption mechanism, the self-immobilization mycelial pellets were prepared using a marine-derived fungus Aspergillus niger ZJUBE-1, and an azo dye, Congo red was chosen as a model dye to investigate batch decolorization efficiency by pellets. The pellets as biosorbent showed strong salt and acid tolerance in biosorption process. The results for dye adsorption showed that the biosorption process fitted well with models of pseudo-second-order kinetic and Langmuir isotherm, with a maximum adsorption capacity of 263.2 mg·g^(-1) mycelium. During 6 batches of continuous decolorization operation, the mycelial pellets could possess efficient decolorization abilities(>98.5%).The appearance of new peak in the UV–Vis spectral result indicated that the decolorization process may also contain biodegradation. The mechanism studies showed that efficient biosorption ability of pellets only relies on the active zone on the surface of the pellet, which can be enhanced by nutrition supplement or be shifted outward by a reculture process. | Tao Lu Qilei Zhang Shanjing Yao | 2017 | Chinese Journal of Chemical Engineering2017，25，3： | 5 |
| 3 | Repeated-batch Cultivation of Encapsulated Monascus purpureus by Polyelectrolyte Complex for Natural Pigment Production显示文摘In general,productions of natural pigment in submerged microorganism culture were much less than that in solid-state fermentation,because the solid-state culture can provide a support carrier for the mycelium. To improve natural pigment production,the cultivation of Monascus purpureus in submerged encapsulated cell was investigated. Monascus purpureus immobilized in polyelectrolyte complex(PEC) microcapsules,which were pre-pared by sodium cellulose sulphate(NaCS) and poly-dimethyl-diallyl-ammonium chloride(PDMDAAC),was a good substitute for submerged cell culture because it mimicked the solid-state environment. The repeated-batch process with encapsulated cells was studied in flasks and a bubble column. The results indicated that the bubble column was more suitable for the encapsulation culture than the shaking flasks because of its good mass transfer performance and minor shear stress on cells. Owing to the protection of the microcapsule's membrane,Monascus purpureus in microcapsules increased approximately three times over that in free cell culture with negligible cell leakage to the medium. The pigment production in the bubble column finally reached 3.82(OD500) ,which was two times higher than in free cell culture. In addition,the duration of each batch was shortened to 15% of that in free cell culture. | LIU Jiefeng REN Yiran YAO Shanjing | 2010 | Chinese Journal of Chemical Engineering2010，18，6： | 4 |
| 4 | Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii显示文摘A xylanase-producing bacterium, isolated from deep sea sediments, was identified as the cold-adapted marine species Acinetobacter Johnsonii. A cold-adapted marine species Acinetobacter Johnsonii could grow at 4 ℃. The optimum temperature and pH of xylanase from a cold-adapted marine species Acinetobacter Johnsonii were 55 ℃ and pH 6.0. Xylanase from a cold-adapted marine species Acinetobacter Johnsonii remained at 80% activity after incubation for 1 h at 65 ℃. The xylanase activity was 1.2-fold higher in 4% ethanol solution than in ethanol free solution. Gibbs free energy of denaturation, ΔG, was higher in 4% ethanol solution than in ethanol free solution. Thermostable ethanol tolerant xylanase was valuable for bioethanol production by simultaneous saccharification and fermentation process with xylan as a carbon source. | Dongsheng Xue Xuhao Zeng Dongqiang Lin Shanjing Yao | 2019 | Chinese Journal of Chemical Engineering2019，27，5： | 3 |
| 5 | Optimizational production of phenyllactic acid by a Lactobacillus buchneri strain via uniform design with overlay sampling methodology显示文摘A Lactobacillus buchneri GBS3 strain isolated from the traditional Chinese pickles was used for the production of 3-phenyllactic acid(PLA), an important compound with antimicrobial activities against a wide species of grampositive and gram-negative bacteria and some fungi. The growth performance of this strain in the de Man, Rogosa and Sharpe(MRS) medium, the production of metabolites of valuable organic acids, and the biosynthesis of PLA using this strain as the whole-cell biocatalyst and phenylpyruvic acid(PPA) as the precursor, were investigated experimentally. The uniform design method with overlay sampling was developed for the optimization of the biotransformation conditions. The results showed that although it produced naturally lactic acid with the maximum concentration of 1.84 g·L^(-1) and PLA with the concentration of 0.015 g·L^(-1) after 66 to 72 h cultivation in MRS broth by fermentation, the present strain displayed an effective utilization ability by transforming PPA to PLA. By the uniform design method with overlay sampling for the design and optimization of transformation conditions, a maximum yield of 10.93 g·L^(-1) PLA with the mole conversion ratio of 83.07% from PPA to PLA was achieved under the optimized condition, i.e., 20 g·L^(-1) glucose, 270 g·L^(-1) cells, 13 g·L^(-1) PPA, pH 8.0 and the reaction time of 15 h, indicating that Lactobacillus buchneri GBS3 was an interesting strain for the biosynthesis of PLA via the microbial transformation. The prediction of PLA yield under different conditions was achieved successfully based on the limited information of only a small number of experiments by the uniform design with overlay sampling. Therefore, the present methodology is effective and helpful for the optimization of the biosynthesis processes of PLA. | Jintao Guan Chaofei Han Yixin Guan Songhong Zhang Junxian Yun Shanjing Yao | 2019 | Chinese Journal of Chemical Engineering2019，27，2： | 3 |
| 6 | Permeabilization of Escherichia coli with ampicillin for a whole cell biocatalyst with enhanced glutamate decarboxylase activity显示文摘The activity of whole-cell biocatalysts is strongly compromised by the cell envelope, which is a permeability barrier against the diffusion of substrates and products. Although common chemical or physical permeabilization methods used in cultured cells enhance cell permeability, these methods inevitably add several extra processing steps after cell cultivation, as well as impede large scale processing. To increase membrane permeability and cellbound glutamate decarboxylase(GAD) activity of recombinant Escherichia coli(BL21(DE3)-p ET28a-gad B) cells without the need for an additional permeabilization step, we investigated the permeabilizing effects of adding cell wall synthesis inhibitors or surfactants to the culture media. Ampicillin was the most effective at improving cell-bound GAD activity of the BL21(DE3)-p ET28a-gad B, although it decreased the cell biomass yield. The best permeabilization effect was observed using an ampicillin concentration of 5 μg·ml-1. Using this concentration,the cell biomass did decrease by 40.58%, but the cell-bound GAD activity of BL21(DE3)-p ET28a-gad B and total cell-bound GAD activity per milliliter of culture was enhanced by 6.24- and 3.64-fold, respectively. Treatment of BL21(DE3)-p ET28a-gad B cells with 5 μg·ml-1ampicillin resulted in structural changes to the cell envelope,but did not substantially affect GAD expression. By entrapping the ampicillin-treated cells in an open pore gelation matrix, which is a polymer derived from polyvinyl alcohol(PVA), alginate, and boric acid, the transformation rate of γ-aminobutyric acid(GABA) at the 10 th cycle produced by immobilized and permeabilized cells remained 46% of the first cycle. GAD activity of the immobilized, permeabilized cells remained over 90% after30 days of storage at 4 °C. | Weirui Zhao Sheng Hu Jun Huang Piyu Ke Shanjing Yao Yinlin Lei Lehe Mei Jinbo Wang | 2016 | Chinese Journal of Chemical Engineering2016，24，7： | 2 |
| 7 | Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins显示文摘The importance of immunoglobulin Y(IgY) as a specific antibody equivalent to mammalian immunoglobulin G(IgG) is well recognized. However, production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study, adsorption behaviors of Ig Y on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine(MEP), 2-mercapto-1-methyli-midazole(MMI), 5-aminobenzi-midazole(ABI) and tryptophan-5-aminobenzi-midazole(W-ABI) were evaluated. The results showed that high adsorption ratio were found at p H 6.0–7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with Ig Y purity of 95% and recovery of 90% was developed after optimization of loading and elution p H and injection volume. The biological activity of the purified Ig Y was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of Ig Y from crude feedstock. | Yingdi Luo Qilei Zhang Shanjing Yao Dongqiang Lin | 2019 | Chinese Journal of Chemical Engineering2019，27，3： | 2 |
| 8 | Spatiotemporal pharmacometabolomics based on ambient mass spectrometry imaging to evaluate the metabolism and hepatotoxicity of amiodarone in HepG2 spheroids显示文摘Three-dimensional(3D)cell spheroid models combined with mass spectrometry imaging(MSI)enables innovative investigation of in vivo-like biological processes under different physiological and pathological conditions.Herein,airflow-assisted desorption electrospray ionization-MSI(AFADESI-MSI)was coupled with 3D HepG2 spheroids to assess the metabolism and hepatotoxicity of amiodarone(AMI).High-coverage imaging of>1100 endogenous metabolites in hepatocyte spheroids was achieved using AFADESI-MSI.Following AMI treatment at different times,15 metabolites of AMI involved in Ndesethylation,hydroxylation,deiodination,and desaturation metabolic reactions were identified,and according to their spatiotemporal dynamics features,the metabolic pathways of AMI were proposed.Subsequently,the temporal and spatial changes in metabolic disturbance within spheroids caused by drug exposure were obtained via metabolomic analysis.The main dysregulated metabolic pathways included arachidonic acid and glycerophospholipid metabolism,providing considerable evidence for the mechanism of AMI hepatotoxicity.In addition,a biomarker group of eight fatty acids was selected that provided improved indication of cell viability and could characterize the hepatotoxicity of AMI.The combination of AFADESI-MSI and HepG2 spheroids can simultaneously obtain spatiotemporal information for drugs,drug metabolites,and endogenous metabolites after AMI treatment,providing an effective tool for in vitro drug hepatotoxicity evaluation. | Limei Li Qingce Zang Xinzhu Li Ying Zhu Shanjing Wen Jiuming He Ruiping Zhang Zeper Abliz | 2023 | Journal of Pharmaceutical Analysis2023，13，5： | 2 |
| 9 | Preparation and evaluation of mixed-mode resins with tryptophan analogues as functional ligands for human serum albumin separation显示文摘Five tryptophan analogues with a hydrophobic indole ring and an amino group on each molecule were used as functional ligands of mixed-mode resins for human serum albumin(HSA) purification. Their adsorption performance was evaluated and the effects of p H and salt addition on HSA adsorption were studied. The resins prepared showed typical p H-dependent adsorption and the highest adsorption capacity and affinity were found at pH 5.0for all the resins tested. The saturated adsorption capacity was 138.02 mg·g^(-1)with the tryptaminefunctionalized resin, which significantly decreased at p H below 4.0 due to electrostatic repulsion between ligands and HSA. Moreover, the addition of Na Cl or(NH_4)_2SO_4in media reduced HSA adsorption capacity, although the two salts showed different affecting profiles. The tryptamine-functionalized resin showed the best salt-tolerant performance, and its high adsorption capacity was maintained under high salt concentrations. In addition, the five resins prepared showed good adsorption selectivity for recombinant HSA from Pichia pastoris broth. Molecular docking results between tryptamine and HSA indicated that tryptamine was favorable to bind on Site II(indole-binding site) of HSA. | Qici Wu Qilei Zhang Shiwen Xu Cheng ong Ge Shanjing Yao Dongqiang Lin | 2017 | Chinese Journal of Chemical Engineering2017，25，7： | 2 |
| 10 | Removal of dyes from wastewater by growing fungal pellets in a semi-continuous mode显示文摘 | Tao Lu Qilei Zhang Shanjing Yao | 2017 | Frontiers of Chemical Science and Engineering2017，11，3： | 2 |
| 11 | Comparative proteomics analysis of OsNAS1 transgenic Brassica napus under salt stress显示文摘Salt stress is one of the major abiotic stresses in agricultural plants worldwide.We used proteomics to analyze the differential expression of proteins in transgenic OsNAS1 and non-transformant Brassica napus treated with 20 mmol/L Na2CO3.Total protein from the leaves was extracted and separated through a high-resolution and highly repetitive two-dimensional electrophoresis(2-DE)technology system.Twelve protein spots were reproducibly observed to be upregulated by more than 2-fold between transgenic and non-transformant B.napus.These 12 spots were digested in-gel with trypsin and characterized by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)to obtain the peptide mass fingerprints.Protein database searching revealed that 5 of these proteins are involved in salt tolerance:dehydrogenase,glutathione S-transferase,peroxidase,20S proteasome beta subunit,and ribulose-1,5-bisphosphate carboxylase/oxygenase.The potential functions of these identified proteins in substance and energy metabolism,stress tolerance,protein degradation,and cell defense are discussed.The salt tolerance of the transgenic rapeseed was significantly improved by the introduction of the OsNAS1 gene from Brazilian upland rice of Oryza sativa(cv.IAPAR 9). | KONG Fang MAO ShanJing DU Kun WU Mian ZHOU XiaoYan CHU ChengCai WANG YouPing | 2011 | Chinese Science Bulletin2011，56，22： | 2 |
| 12 | Experimental and in silico studies on three hydrophobic charge-induction adsorbents for porcine immunoglobulin purification显示文摘Three hydrophobic charge-induction adsorbents with functional ligands of 4-mercapto-ethyl-pyridine, 2-mercapto-methyl-imidazole or 2-mercapto-benzimidazole were evaluated in the purification of porcine immunoglobulin from porcine blood. Adsorption isotherms were studied under different pH conditions. The adsorbent with 2-mercapto-methyl-imidazole as the ligand showed reasonable adsorption capacity(43.60 mg·g^(-1)gel)with great selectivity and it also showed the best elution performance in chromatographic studies. A multi-pH step elution process was proposed for the 2-mercapto-methyl-imidazole adsorbent, and the results showed that high immunoglobulin purity(94.3%) and a yield of 9.8 mg·(ml plasma)^(-1) could be achieved under the optimal condition of loading(pH 5.0)–pre-elution(pH 7.0)–elution(pH 3.8). Moreover, molecular simulation was employed to help in analyzing the binding mechanism between the ligands and immunoglobulin, and the results showed that both 2-mercapto-benzimidazole and 2-mercapto-methyl-imidazole ligands were docked on the same pocket(around TYR319 and LEU309) of the Fc fragment of immunoglobulin, with 2-mercaptobenzimidazole showing stronger binding interactions. | Qilei Zhang Tiantian Zhuang Hongfei Tong Hongyin Wang Dongqiang Lin Shanjing Yao | 2016 | Chinese Journal of Chemical Engineering2016，24，1： | 2 |
| 13 | Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity显示文摘The cellulase cocktail produced by marine Aspergillus niger exhibits a property of salt-tolerance,which is of great potential in cellulose degradation in high salt environment.In order to explain the mechanism on the salttolerance of the cellulase cocktail produced by marine A.niger,six cellulase components(AnCel6,AnCel7A,AnCel7B,AnEGL,AnBGL1 and AnBGL2)were obtained by directed expression.Studies on their enzymatic properties revealed that oneβ-glucosidase(AnBGL2)and one endoglucanase(AnEGL)exhibited an outstanding salttolerant property,and one cellobiohydrolase(AnCel7B)exhibited a certain salt-tolerant property.Subsequent study revealed that the salt-tolerant An EGL and AnCel7B endowed the cellulase cocktail with stronger salttolerant property,while the salt-tolerant An BGL2 had no positive effect.Moreover,after overexpression of AnCel6,AnCel7A,AnCel7B and AnEGL,the activity of cellulase cocktail increased by 80%,70%,63%and 68%,respectively.However,the activity of cellulase cocktail was not improved after overexpression of AnBGL1 and AnBGL2.After mixed-strain fermentation with cellobiohydrolase recombinants(cel6 a,cel7a and cel7b recombinants)and endoglucanase recombinant(egl recombinant),the the activity of cellulase cocktail increased by 114%,102%and91%,respectively. | Linian Cai Shengnan Xu Tao Lu Dongqiang Lin Shanjing Yao | 2020 | Chinese Journal of Chemical Engineering2020，28，4： | 2 |
| 14 | Behavior of concrete filled tubes and confined concrete filled tubes under high speed impact 显示文摘 | ShanJ H Chen R Zhang W X | 2007 | Advances in Structural Engineering2007，10，2： | 1 |
| 15 | A channel-confined strategy for synthesizing CoN-CoO_(x)/C as efficient oxygen reduction electrocatalyst for advanced zinc-air batteries显示文摘Designing hybrid transition metal compounds with optimized electronic structure and firmly dispersing them on a matrix to avoid aggregation and shedding is of great significance for achieving high electrocatalytic performances.Herein,an adsorption-complexation-calcination strategy based on channel confining effect is explored to obtain CoN-CoO_(x)hybrid nanoparticles uniformly dispersed in mesoporous carbon.The CoN-CoO_(x)/C composite exhibits excellent electrocatalytic behavior for oxygen reduction reaction(ORR).The half-wave potential and durability are comparable or superior to those of Pt/C.When applying as cathode catalyst for a primary zinc-air battery,the open-circuit voltage and peak power density reach up to 1.394 V and 109.8 mW·cm^(−2),respectively.A high gravimetric energy density of 950.3 Wh·kgZn^(−1) is delivered at 10 mA·cm^(−2) with good rate capability and stability.Density functional theory(DFT)calculation demonstrates the favorable ORR intermediate adsorbability and metallic characteristics of CoN grains with oxide hybridization to optimize the electronic structure.This work provides a facile adjustable approach for obtaining highly dispersed nanoparticles with controllable hybrid composition on a substrate,which is important for future design and optimization of high-performance electrocatalysts. | Xingmei Guo Wei Zhang Jing Shi Mengting Duan Shanjing Liu Junhao Zhang Yuanjun Liu Shenglin Xiong Qinghong Kong | 2022 | Nano Research2022，15，3： | 1 |
| 16 | Morphine and methadone have different effects on calcium channel currents in neuroblastoma cells 显示文摘 | YangJC ShanJ Ng KF | 2000 | Brain Res2000，870，122： | 1 |
| 17 | Mitogen-activated protein kinase inhibitors improve heart function and prevent fibrosis in cardiomyopathy caused by mutation in lamin AIC gene显示文摘 | Wu W Muchir A ShanJ | 2011 | Circulation2011，123，1： | 1 |
| 18 | Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption显示文摘The mixed-mode resins for protein adsorption have been prepared by a novel strategy, copolymer grafting. Specially, the copolymer-grafted resins CG-MA with two functional groups, 5-amino-benzimidazole (ABI) and methacryloxyethyltrimethyl ammonium chloride (METAC), have been prepared through surfaceinitiated activator generated by electron transfer for atom transfer radical polymerization of METAC and glycidyl methacrylate (GMA), followed by a ring-open reaction to introduce ABI. The charge and hydrophobicity of CG-MA resins could be controlled by manipulating the addition of METAC and GMA/ABI. Besides, METAC and ABI provided positive effects together in both protein adsorption and elution: dynamic binding capacity of human Immunoglobulin G (hlgG) onto CG-M-A resin with the highest ligand ratio of METAC to ABI is 46.8 mg-g 1 at pH 9 and the elution recovery of hlgG is 97.0% at pH 5. The separation experiment showed that purity and recovery of monoclonal antibody from cell culture supernatant are 96.0% and 86.5%, respectively, indicating that copolymer-grafted mixed-mode resins could be used for antibody purification. | Shenggang Chen Tao Liu Ruiqi Yang Dongqiang Lin Shanjing Yao | 2019 | Frontiers of Chemical Science and Engineering2019，13，1： | 1 |
| 19 | Senentation and Reconstruction of Polyhedral Building Root from Aerial LiDAR Point Clouds显示文摘 | Sampath A ShanJ | 2010 | IEEE Trans on Geoscience and Remote Sensing2010，48，3： | 1 |
| 20 | A novel β-cyclodextrin polymer/tungsten carbide composite matrix for expanded bed adsorption: preparation and characterization of physical properties 显示文摘 | Zhao Jun Lin Dongqiang Wang Yuchong Yao Shanjing | 2010 | Carbohydrate Polymers2010，80，： | 1 |
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