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| 1 | Application of Two-Dimensional Electrophoresis in the Research of Retinal Proteins of Diabetic Rat显示文摘Diabetes mellitus (DM) is a chronic disease which is associated with numerous serious health complications such as diabetic retinopathy, and is the leading cause of new cases of blindness in adults at the age of 20-74 years old. The aim of the study was to establish and optimize a two-dimensional polyacrylamide gel electrophoresis (2-DE) technique for retina proteomics to improve the resolution and reproducibility, and to observe the proteomic changes of retinal tissues in diabetic and normal rats. Proteins were extracted from retinal tissues of normal and 8 weeks diabetic SD rats and used in two-dimensional electrophoresis. Various conditions of retina proteomic 2-DE were adjusted, optimized and protein spots of differential expression were obtained through analysis of 2-DE images with PDQuest software. By choosing appropriate sample amount, using pre-cast IPG dry strips (pH 5-8) and casting 12% equal gel, satisfactory 2-DE images of retina were obtained and a steady 2-DE technique was established. In this way, we found 36 spots in 2-DE gel of diabetic retinas that exhibited statistically significant variations, including up-regulation of 5 proteins in diabetic rat retinas, down-regulation of 23, and disappearance of 8, in comparison with normal tissues. The differences of protein expression were observed in retinas between diabetic and normal rats. Our established 2-DE technique of retina proteins could be effectively applied in proteomics of retina diseases. | Shangqing Liu Yanyan Zhang Xianyong Xie Weiming Hu Rong Cai Jian Kang Huijun Yang | 2007 | Cellular & Molecular Immunology2007，4，1： | 9 |
| 2 | Gut Microbiota Dysbiosis Strengthens Kupffer Cell-mediated Hepatitis B Virus Persistence through Inducing Endotoxemia in Mice显示文摘Background and Aims:Change of gut microbiota com-position is associated with the outcome of hepatitis B virus(HBV)infection,yet the related mechanisms are not fully characterized.The objective of this study was to investigate the immune mechanism associated with HBV persistence induced by gut microbiota dysbiosis.Methods:C57BL/6 mice were sterilized for gut-microbiota by using an antibi-otic(ABX)mixture protocol,and were monitored for their serum endotoxin(lipopolysaccharide[LPS])levels.An HBV-replicating mouse model was established by performing HBV-expressing plasmid pAAV/HBV1.2 hydrodynamic injec-tion(HDI)with or without LPS,and was monitored for se-rum hepatitis B surface antigen,hepatitis B e antigen,HBV DNA,and cytokine levels.Kupffer cells(KCs)were purified from antibiotic-treated mice and HBV-replicating mice and analyzed for IL-10 production and T cell suppression ability.Results:ABX treatment resulted in increased serum LPS levels in mice.The KCs separated from both ABX-treated and LPS-treated HBV-replicating mice showed significantly increased IL-10 production and enhanced ability to suppress IFN-γproduction of TCR-activated T cells than the KCs sep-arated from their counterpart controls.HDI of pAAV/HBV1.2 in combination with LPS in mice led to a delayed HBV clear-ance and early elevation of serum IL-10 levels compared to pAAV/HBV1.2 HDI alone.Moreover,IL-10 function blockade or KC depletion led to accelerated HBV clearance in LPS-treated HBV-replicating mice.Conclusions:Our results suggest that dysbiosis of the gut microbiota in mice leads to endotoxemia,which induces KC IL-10 production and strengthens KC-mediated T cell suppression,and thus fa-cilitates HBV persistence. | Wenqing Zhou Jinzhuo Luo Xiaohong Xie Shangqing Yang Dan Zhu Hongming Huang Dongliang Yang Jia Liu | 2022 | Journal of Clinical and Translational Hepatology2022，10，1： | 4 |
| 3 | Research on ultra-small textured surface of multicrystalline silicon solar cell显示文摘The ultra-small textured surface of multicrystalline silicon solar cell,prepared by electroless chemical-etching method,shows an excellent anti-reflection property over a wide spectral bandwidth.A novel back surface protection method and front surface passivation method have been used in the multicrystalline solar cells with ultra-small textured surfaces.With these improvements,the back surface remains intact after the etch process and the efficient minority lifetime is apparently increased.The test result shows that the solar cell with ultra-small textured surface can obtain better electrical performances by these improvements. | LI HaoFeng JIA Rui DOU BingFei CHEN Chen XING Zhao YANG YongZhou DING WuChang MENG YanLong LIU XinYu YE TianChun LI ShangQing | 2013 | Science China(Technological Sciences)2013，56，4： | 2 |
| 4 | A hollow tubular NiCo layacknered double hydroxide@Ag nanowire structure for high-power-density flexible aqueous Ni//Zn battery显示文摘Flexible aqueous Ni//Zn batteries have attracted much attention as promising candidates for energy storage in the field of flexible electronics.However,the Ni-based cathodes still face the challenges of poor conductivity,confined charge/mass transfer,and non-flexibility.In this work,we designed a hollow tubular structure consisting of a conductive silver nanowire (Ag NW) wrapped by active Ni Co layered double hydroxide (LDH),for enhancing the electrical conductivity,improving the charge/mass transfer kinetics,and facilitating the ion penetration.By optimizing the contents of Ni,Co and Ag NW,the Ni_(4)Co LDH@Ag_(1.5)NW composite shows a maximum specific capacity of 115.83 m Ah g^(-1)at 0.1 A g^(-1)measured in a two-electrode system.Highlightingly,the flexible aqueous Ni//Zn battery assembled by Ni_(4)Co LDH@Ag_(1.5)NW interwoven with multi-walled carbon nanotube cathode and Zn foil anode realizes a high power density of 160μW cm^(-2)at the energy density of 23.14μWh cm^(-2),which is superior compared with those of oxide/hydroxide based devices and even higher than those of many carbon-based supercapacitors,showing its promising potentials for flexible energy storage applications. | Xiaoyang Xuan Min Qian Likun Pan Ting Lu Yang Gao Lu Han Lijia Wan Yueping Niu Shangqing Gong | 2022 | Journal of Energy Chemistry2022，31，7： | 0 |
| 5 | Rare-Earth Doping Graphitic Carbon Nitride Endows Distinctive Multiple Emissions with Large Stokes Shifts显示文摘Two-dimensional(2D)graphitic carbon nitride(g-C_(3)N_(4))possesses a unique geometric configuration featuring a superimposed heterocyclic sp2 carbon and nitrogen framework.Its fluorescence may be attributed to π-π^(*),lone pair(LP)-π^(*),or LP-δ^(*)transitions.So far,the manipulation of its fluorescence emissions is largely unexploited and remains challenging.Herein,for the first time,rare-earth doping into the backbone structure of a g-C_(3)N_(4) framework under microwave agitation endows unprecedented fluorescence nature,with the emergence of two exceptional new fluorescence emissions in the 450-700 nm range.With terbium-doped g-C_(3)N_(4):Tb as a representative,these emissions exhibit distinctive features,that is,very sharp fluorescence peaks with narrow full width at half maximum(FWHM)(peak width at half-height)of<12 nm,quantumyields of 2.3±0.0% and 7.6±0.1% for the new emissions at λ_(ex)/λ_(em)=290/490 nm,and 290/545 nm,respectively;and a large Stokes shift of>200 nm.These features of g-C_(3)N_(4):Tb are most advantageous for applications in various fields,as demonstrated by(1)tracking biodistribution of g-C_(3)N_(4) in vivo with mass spectrometric imaging where the doped terbium serves as a tag,(2)a biometrics study facilitating the identification of an individual through fingerprint,and(3)anti-counterfeiting with g-C_(3)N_(4)∶Tb as a dual-functional marker to facilitate fluorescence and mass spectrometric imaging. | Xun Liu Shangqing Zhang Jinhui Liu Xing Wei Ting Yang Mingli Chen Jianhua Wang | 2022 | CCS Chemistry2022，4，6： | 0 |
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