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| 1 | 采用PCR-DGGE技术分析蛋鸡肠道细菌种群结构及多样性显示文摘利用基于16S rDNA的PCR-DGGE(变性梯度凝胶电泳)图谱技术结合特异性和共性条带割胶回收DNA进行克隆和测序,对2、4、6和8周龄蛋鸡嗉囊、十二指肠、空肠、回肠和盲肠内容物细菌群落的结构和多样性进行了比较,并鉴定了8周龄蛋鸡部分特异性和共性群落成员,分析蛋鸡年龄和肠段部位对微生物群落结构和多样性的影响。肠道菌群DGGE图谱显示肠道部位对细菌种群结构影响很大,盲肠内微生物的多样性最高,其次是回肠和空肠,嗉囊和十二指肠的微生物多样性比较低。十二指肠内细菌与其它肠段相比差异最大,其次是盲肠与其它肠段细菌组成的差异。随着蛋鸡周龄的增加,消化道微生物经历了由简单(2周)到复杂(4周),再回复简单(6周)到复杂(8周)的变化过程。DGGE图谱中共性条带序列分析表明8周龄蛋鸡消化道前段的优势细菌是三得利乳杆菌(Lactobacillus suntoryeus)、索氏梭菌(Clostridium sordellii)和大肠杆菌,盲肠中的特异性条带主要是各种不可培养的细菌以及超巨巨单胞菌(Megamonas hypermegale)和梭菌属细菌(Clostridiumspp)。该研究结果表明蛋鸡肠道部位决定细菌群落的结构和多样性,蛋鸡的周龄影响肠道细菌多样性。 | 倪学勤 Joshua Gong Hai Yu 曾东 Shayan Sharif 周小秋 | 2008 | 畜牧兽医学报2008，39，7： | 56 |
| 2 | PCR-DGGE技术分析蛋鸡MHC基因对肠道细菌种群结构的影响显示文摘【目的】研究蛋鸡MHC(Major Histocompatibility Complex,主要组织相容性复合物)基因对不同周龄和不同部位肠道细菌种群结构的影响。【方法】使用基于16S rDNA的PCR-DGGE技术,结合特异性和共性条带割胶回收DNA进行克隆和测序,对2,4,6和8周龄商品蛋鸡和15I5系蛋鸡嗉囊、十二指肠、空肠、回肠和盲肠内含物细菌群落的结构和多样性进行比较,鉴定8周龄蛋鸡部分特异性和共性群落成员。【结果】商品蛋鸡和15I5系蛋鸡盲肠细菌的多样性最高,其次是回肠和空肠,嗉囊和十二指肠的细菌多样性比较低,且随着蛋鸡周龄增加,各肠段细菌多样性呈上升趋势,MHC基因对细菌多样性无显著影响。DGGE图谱聚类分析表明,蛋鸡MHC基因对4、6周龄肠道细菌的影响最大,对2周龄影响最小;而且MHC基因对消化道前段细菌的影响小于对后段影响。序列分析发现,8周龄商品蛋鸡和15I5系蛋鸡均有大量的三得利乳杆菌(Lactobacillus suntoryeus),是蛋鸡消化道前段的优势细菌;商品蛋鸡空肠、回肠和盲肠中的特异性条带主要是Ochrobactrum spp,索氏梭菌(Clostridium sordellii),超巨巨单胞菌(Megamonas hypermegale)和大量不可培养的细菌;15I5系蛋鸡的特异性条带主要是不可培养的细菌。【结论】蛋鸡MHC基因可影响不同周龄、不同肠段细菌群落的结构。 | 倪学勤 Joshua Gong Hai Yu Shayan Sharif 曾东 | 2009 | 中国农业科学2009，42，7： | 13 |
| 3 | Development of a real-time PCR assay using SYBR Green chemistry for monitoring Marek’s disease virus genome load in feather tips显示文摘 | Mohamed Faizal Abdul-Careem Bruce D. Hunter éva Nagy Leah R. Read Babak Sanei J. Lloyd Spencer Shayan Sharif | 2005 | Journal of Virological Methods2005，，1： | 1 |
| 4 | Within-host model of respiratory virus shedding and antibody response to H9N2 avian influenza virus vaccination and infection in chickens显示文摘Avian influenza virus(AIV)H9N2 subtype is an infectious pathogen that can affect both the respiratory and gastrointestinal systems in chickens and continues to have an important economic impact on the poultry industry.While the host innate immune response provides control of virus replication in early infection,the adaptive immune response aids to clear infections and prevent future invasion.Modelling virus-innate immune response pathways can improve our understanding of early infection dynamics and help to guide our understanding of virus shedding dynamics that could lead to reduced transmission between hosts.While some countries use vaccines for the prevention of H9N2 AIV in poultry,the virus continues to be endemic in regions of Eurasia and Africa,indicating a need for improved vaccine efficacy or vaccination strategies.Here we explored how three type-I interferon(IFN)pathways affect respiratory virus shedding patterns in infected chickens using a within-host model.Additionally,prime and boost vaccination strategies for a candidate H9N2 AIV vaccine are assessed for the ability to elicit seroprotective antibody titres.The model demonstrates that inclusion of virus sensitivity to intracellular type-I IFN pathways results in a shedding pattern most consistent with virus titres observed in infected chickens,and the inclusion of a cellular latent period does not improve model fit.Furthermore,early administration of a booster dose two weeks after the initial vaccine is administered results in seroprotective titres for the greatest length of time for both broilers and layers.These results demonstrate that type-I IFN intracellular mechanisms are required in a model of respiratory virus shedding in H9N2 AIV infected chickens,and also highlights the need for improved vaccination strategies for laying hens. | Xiao-Ting Xie Alexander Yitbarek Jake Astill Shirene Singh Salah Uddin Khan Shayan Sharif Zvonimir Poljak Amy L.Greer | 2021 | Infectious Disease Modelling2021，6，1： | 0 |
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