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| 1 | Muscle RING-finger protein-1 (MuRFl) functions and cellular localization are regulated by SUMOl post-translational modification显示文摘The muscle RING-finger protein-1 (MuRFl) is an E3 ubiquitin ligase expressed in skeletal and cardiac muscle tissues and it plays important roles in muscle remodeling. Upregulation of MuRFl gene transcription participates in skeletal muscle atrophy, on contrary downregulation of protein expression leads to cardiac hypertrophy. MuRFl gene point mutations have been found to generate protein aggregate myopathies defined as muscle disorder characterized by protein accumulation in muscle fibers. We have discovered that MuRFl turned out to be also a target for a new post-translational modification arbitrated by conjugation of SUM01 and it is mediated by the SUMO ligases E2 UBC9 and the E3 PIASy/4. SUMOylation takes place at lysine 238 localized at the second coiled-coil protein domain that is required for efficient substrate interaction for polyubiquitination. We provided evidence that SUMOylation is essential for MuRFl nuclear translocation and its mitochondria accumulation is enhanced in hyper? glycemic conditions delivering a stabilization of the overall SUMOylated proteins in cultured myocytes. Thus, our findings add this SUM01 post-translational modification as a new concept to understand muscle disorders related to the defect in MuRFl activity. | Gabriel Heras Arvind Venkat Namuduri Leonardo Traini Ganna Shevchenko Alexander Falk Sara Bergstrom Lind Mi Jia Geng Tian Stefano Gastaldello | 2019 | Journal of Molecular Cell Biology2019，11，5： | 4 |
| 2 | TNF-α gene polymorphisms: association with age-related macular degeneration in Russian population显示文摘AIM: To study polymorphisms in promotor regions of tumor necrosis factor(TNF)-α TNF-863 A/C(rs1800630), TNF-308 A/G(rs1800629), and TNF-238 A/G(rs361525) in patients with age-related macular degeneration(AMD) and associations of complex TNF-α genotypes with AMD. METHODS: One hundred and two patients(82 women, 20 men; mean age 64.2±1.2 y) with AMD and 100 healthy age-and sex-matched controls(82 women, 18 men; 60±1.4 y) were included in the study. All subjects were Caucasian, all subjects and their parents were inhabitants of Russia. Genomic DNA was obtained from EDTA-preserved blood using the standard phenol-chloroform method. Polymorphisms were detected by polymerase chain reaction followed by the restriction fragment length polymorphism method. The following TNF-α genotypes were studied: TNF-α-238 AA, GA, GG, TNF-α-308 AA, GA, GG, TNF-α-863 AA, CA, CC. RESULTS: Differences in TNF-α-863 and TNF-α-238 genotypes frequencies in patients with AMD and healthy controls were not found. The distribution of TNF-α-308 AA and TNF-α-308 GA genotypes was significantly different between the studied group and the controls [odds ratios(OR) =0.22, P=0.0287 and OR=2.91, P=0.0063, respectively]. TNF-863 CC/TNF-308 GA and TNF-308 GA/TNF-238 GG genotypes were associated with the increased risk of AMD(OR=2.48, P=0.0332 and OR=2.51, P=0.0187, respectively). Five genotypes combinations appeared to be protective. CONCLUSION: In the present study, single nucleotide polymorphisms and complex polymorphisms of one of the key inflammatory cytokines TNF-α, and a number of significant associations of these polymorphisms with AMD in Russian population have been shown. Complexanalysis of genotypes could be important in AMD risk factors detection and studying pathogenesis. | Valeriy Chernykh Alla Shevchenko Vladimir Konenkov Viktor Prokofiev Alena Eremina Alexander Trunov | 2019 | International Journal of Ophthalmology(English edition)2019，12，1： | 2 |
| 3 | 连铸结晶器插入钢带对连铸坯质量的影响显示文摘中心偏析和等轴晶率是评价连铸坯内部质量的重要参数,而用于改善内部质量的电磁搅拌、微合金化等技术相对复杂,实施成本高。通过对结晶器插入钢带,通过钢带的熔化吸热有效地降低结晶器内钢液过热度,可以提高铸坯内部质量。讨论了在给定工艺条件下钢带插入对连铸坯质量的影响。计算和生产试验结果表明,插入钢带可以明显降低钢水过热度;插入钢带的连铸坯疏松和等轴晶率等指标均比没有插钢带的连铸坯好,且吨钢插入钢带量增加可以增加等轴晶率,有利于改善钢坯内部质量。 | 于林辉 张振山 Shevchenko Anthony 沈明钢 王一雍 | 2015 | 钢铁2015，50，11： | 2 |
| 4 | Effect of the size f actor and alloying on oxidation of aluminum powders 显示文摘 | Shevchenko V G Kononenko V I Latosh I N | 1994 | Combustion Explosio n and Shock Waves1994，30，5： | 2 |
| 5 | Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels 显示文摘 | Shevchenko A Wilm M Vorm O | 1996 | Anal Chem1996，68，： | 2 |
| 6 | A generic protein purification method for protein complex characterization and proteome exploration显示文摘 | Rigaut G Shevchenko A Rutz B | 1999 | Nat Biotechnol1999，17，10： | 1 |
| 7 | Reverse transcriptase motify in the catalytic subunit of telomerase显示文摘 | Lingner J Hughes TR Shevchenko A | 1997 | Science1997，276，： | 1 |
| 8 | Achieving high free electron mobility in ZnO: A1 thin films grown by reactive pulsed magnetron sputtering 显示文摘 | Cornelius S Vinnichenko M Shevchenko N | 2009 | Applied Physcis Letters2009，94，04： | 1 |
| 9 | The use of lidocaine for preventing the withdrawal associated with the injection of rocuronium in children and adolescents显示文摘 | Shevchenko Y Jocson JC McRae VA | 1999 | Anesth Analg1999，88，4： | 1 |
| 10 | A Sm-like protein complex that participates in mRNA degradation显示文摘 | BOUVERET E RIGAUT G SHEVCHENKO A | 2000 | EMBO J2000，19，7： | 1 |
| 11 | Neoangiogenesis and coronary atheroscle-rosis:diagnostic value of a novel biochemical marker pla-centa growth factor in patients with ischemic heart disease显示文摘 | SHEVCHENKO A O SHEVCHENKO O P ORLO-VA O V | 2006 | Kardiologiia2006，46，： | 1 |
| 12 | Depoly merization of Lignin in Wood withMolecular Hydrogen Iodid 显示文摘 | Sergey M Shevchenko | 2000 | Croatica Chemica Acta2000，73，3： | 1 |
| 13 | A generic protein purification method for protein complex characterization and proteome exploration 显示文摘 | Rigaut G Shevchenko A Rutz B | 1999 | Nat Biotechnol1999，17，10： | 1 |
| 14 | A 3-D Monte-Carlo (Potts) model for recrystallization and grain growth in polycrystalline materials 显示文摘 | Ivasishin O M Shevchenko S V Vasiliev N L | 2006 | Materials Science and Engineering A2006，433，12： | 1 |
| 15 | A synthesis of tritium-labeled olanzapine 显示文摘 | Shevchenko VP Nagaev IY Kuznetsov YV | 2005 | Russ J Bioorg Chem2005，31，4： | 1 |
| 16 | Model uncertainty in claims reserving within Tweedie's com- pound Poisson models显示文摘 | Peters G W Shevchenko P V Wtithrich M V | 2009 | Astin Bulletin2009，39，01： | 1 |
| 17 | Mass spectrometric sequen- cing of proteins silver-stained polyacrylamide gels 显示文摘 | Shevchenko A Wilm M Vorm O | 1996 | Anal Chem1996，68，5： | 1 |
| 18 | Tritium labeling of bioorganie compounds by isotope exchange显示文摘 | Shevchenko V Nagaev I Myasoedov N | 2012 | Radiochemistry2012，54，1： | 1 |
| 19 | Peptide Sequencing by Mass Spectrometry for Homology Searches and Cloning of Genes显示文摘 | Andrej Shevchenko Matthias Wilm Matthias Mann | 1997 | Journal of Protein Chemistry1997，，5： | 1 |
| 20 | FLICE, A Novel FADD-Homologous ICE/CED-3–like Protease, Is Recruited to the CD95 (Fas/APO-1) Death-Inducing Signaling Complex显示文摘 | Marta Muzio Arul M Chinnaiyan Frank C Kischkel Karen O’Rourke Andrej Shevchenko Jian Ni Carsten Scaffidi James D Bretz Mei Zhang Reiner Gentz Matthias Mann Peter H Krammer Marcus E Peter Vishva M Dixit | 1996 | Cell1996，，： | 1 |
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