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| 1 | A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming. | Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng | 2019 | Cell Research2019，29，9： | 9 |
| 2 | High-throughput droplet microfluidic synthesis of hierarchical metal-organic framework nanosheet microcapsules显示文摘Using two-dimensional(2D)metal-organic framework(MOF)nanosheets as new building blocks to create more complex architectures at the mesoscopic/macroscopic scale has attracted extensive interest in recent years.Nevertheless,it remains a great challenge to assemble MOF nanosheets into hierarchical hollow structures so far.In this paper,we describe a successful example of hierarchical MOF nanosheet microcapsules,with precisely controlled sizes,produced on large scale within minutes with a continuous droplet microfluidic strategy.Following a reaction/diffusion growth mechanism,the microcapsule shells feature a continuous smooth inner layer and a porous outer layer.Such hierarchical structure enables the encapsulation of magnetite nanoparticles inside and loading of dense gold nanoparticles outside the microcapsules,which exhibit highly efficient heterogeneous catalytic activity and easy recyclability.The present microfluidic assembly method offers a new pathway for preparing hierarchical MOF nanosheet structures,with the potential for extension to the formation of other 2D nanosheets in general. | Songting Wu Zhong Xin Shicheng Zhao Shengtong Sun | 2019 | Nano Research2019，12，11： | 6 |
| 3 | Cancer metabolism and tumor microenvironment:fostering each other?显示文摘The changes associated with malignancy are not only in cancer cells but also in environment in which cancer cells live.Metabolic reprogramming supports tumor cells’high demand of biogenesis for their rapid proliferation,and helps tumor cells to survive under certain genetic or environmental stresses.Emerging evidence suggests that metabolic alteration is ultimately and tightly associated with genetic changes,in particular the dysregulation of key oncogenic and tumor suppressive signaling pathways.Cancer cells activate HIF signaling even in the presence of oxygen and in the absence of growth factor stimulation.This cancer metabolic phenotype,described firstly by German physiologist Otto Warburg,ensures enhanced glycolytic metabolism for the biosynthesis of macromolecules.The conception of metabolite signaling,i.e.,metabolites are regulators of cell signaling,provides novel insights into how reactive oxygen species(ROS)and other metabolites deregulation may regulate redox homeostasis,epigenetics,and proliferation of cancer cells.Moreover,the unveiling of noncanonical functions of metabolic enzymes,such as the moonlighting functions of phosphoglycerate kinase 1(PGK1),reassures the importance of metabolism in cancer development.The metabolic,microRNAs,and ncRNAs alterations in cancer cells can be sorted and delivered either to intercellular matrix or to cancer adjacent cells to shape cancer microenvironment via media such as exosome.Among them,cancer microenvironmental cells are immune cells which exert profound effects on cancer cells.Understanding of all these processes is a prerequisite for the development of a more effective strategy to contain cancers. | Yiyuan Yuan Huimin Li Wang Pu Leilei Chen Dong Guo Hongfei Jiang Bo He Siyuan Qin Kui Wang Na Li Jingwei Feng Jing Wen Shipeng Cheng Yaguang Zhang Weiwei Yang Dan Ye Zhimin Lu Canhua Huang Jun Mei Hua-Feng Zhang Ping Gao Peng Jiang Shicheng Su Bing Sun Shi-Min Zhao | 2022 | Science China(Life Sciences)2022，65，2： | 5 |
| 4 | Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells显示文摘Background:Tooth is vital not only for a good smile,but also good health.Yet,we lose tooth regularly due to accidents or diseases.An ideal solution to this problem is to regenerate tooth with patients’own cells.Here we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells(ifhU-iPSCs).Results:We first differentiated ifhU-iPSCs to epithelial sheets,which were then recombined with E14.5 mouse dental mesenchymes.Tooth-like structures were recovered from these recombinants in 3 weeks with success rate up to 30%for 8 different iPSC lines,comparable to H1 hESC.We further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures,possessing physical properties such as elastic modulus and hardness found in the regular human tooth.Conclusion:Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies. | Jinglei Cai Yanmei Zhang Pengfei Liu Shubin Chen Xuan Wu Yuhua Sun Ang Li Ke Huang Rongping Luo Lihui Wang Ying Liu Ting Zhou Shicheng Wei Guangjin Pan Duanqing Pei | 2013 | Cell Regeneration2013，2，1： | 4 |
| 5 | Simultaneous estimation of ego-motion and vehicle distance by using a monocular camera显示文摘Auto navigation and vehicle distance estimation are of the critical importance in intelligent vehicles.This paper proposes a novel approach to solve these two problems simultaneously by using a monocular camera.First,we introduce an incremental depth parameterized model to overcome the scale ambiguity problem in monocular vision,which differs from other parameterized models with reduced state-dimension and consistent observability.In addition,the view-field of camera is divided into static and dynamic parts so that the egomotion and vehicle-space can be calculated simultaneously.Outdoor experiments are implemented to validate the effectiveness of the proposed approach. | YANG DongFang SUN FuChun WANG ShiCheng ZHANG JinSheng | 2014 | Science China(Information Sciences)2014，57，5： | 3 |
| 6 | Wood waste biochar promoted anaerobic digestion of food waste:focusing on the characteristics of biochar and microbial community analysis显示文摘Anaerobic digestion(AD)has been considered as a promising technique for food waste(FW)recycling.However,the accumulation of volatile fatty acids(VFAs)restricts the stability of anaerobic reactors.The present study investigated the use of biochar produced at different conditions(750℃-30 min,750℃-60 min,750℃-120 min,550℃-60 min,650℃-60 min,850℃-60 min,950℃-60 min)for enhancing the AD of FW.Batch experiments showed that all the biochar increased the methane production rates and biochar obtained at 750℃-60 min resulted in the highest enhance-ment by 21.5%.It was further showed surface oxygen-containing functional groups and graphitization degree of biochar were the critical factors for improving methane production.Microbial analysis showed that biochar addi-tion formed different microbial communities,and Methanosaeta,Romboutsia,and norank_f_Anaerolineaceae were enriched,which might be correlated with direct interspecies electron transfer(DIET).This research showed biochar could enhance the AD of FW and also revealed the main characteristics of biochar relating with the enhancement of AD. | Chao Zhang Rui Yang Meichen Sun Shicheng Zhang Mingjing He Daniel C.W.Tsang Gang Luo | 2022 | Biochar2022，4，1： | 2 |
| 7 | Effective treatment of SARS-CoV-2-infected rhesus macaques by attenuating inflammation显示文摘Dear Editor,The COVID-19 pandemic caused by the SARS-CoV-2 virus has caused a significant public health crisis worldwide.Recent studies show that excessive inflammatory response is critical for SARS-CoV-2 pathogenesis and COVID-19 severity,'which can lead to acute lung injury(ALI)and acute respiratory distress syndrome(ARDS).2 One major factor for acute inflammation in SARS-CoV-2 infection is the inflammatory macrophages,which has been considered important for the production of large amounts of proinflammatory cytokines.'Autopsy identifhed an intense infltra-tion of macrophages in the lung tissues of fatal COVID-19 patients. | Shuaiyao Lu Jingjing Zhao Jiebin Dong Hongqi Liu Yinhua Zhu Honggang Li Liping Liu Yun Yang Shicheng Sun Yifan Song Yuan Zhao Ruiping She Tuoping Luo Hongkui Deng Xiaozhong Peng | 2021 | Cell Research2021，31，2： | 1 |
| 8 | Robust consensus for networked mechanical systems with coupling time delay显示文摘 | MIN Haibo WANG Shicheng SUN Fuchun | 2012 | International journal of control automation and systems2012，10，2： | 1 |
| 9 | Proton antagonist membrane towards exclusive CO_(2)reduction显示文摘Membrane electrode assembly reactor offers great promise toward practical CO_(2)electrolysis.Unfortunately,traditional proton exchange membrane possesses strong acidic chemical environment,which facilitates undesired hydrogen evolution reaction.Here we report a proton antagonist strategy,through which the proton diffusion pathways have been severely impeded by Na+cation to produce an alkaline-rich environment.With this new membrane electrode assembly,we can significantly suppress the hydrogen evolution and achieve a Faradaic efficiency of 95.7%for CO with 51.5%energy efficiency.In addition,our proton antagonist membrane outperforms the commercial anion exchange membrane in both conductivity and oxidation resistance lifetime,which are crucial for large scale electrolysis of carbon neutral chemicals. | Taishi Xiao Yao Ma Shicheng Zeng Xiang Yao Tong Ye Hongbin Li Wei Li Zhengzong Sun | 2023 | Nano Research2023，16，4： | 0 |
| 10 | Dynamic Recrystallization Behavior of Q370qE Bridge Steel显示文摘Bridge steel has been widely used in recent years for its excellent performance. Understanding the high-temperature Dynamic Recrystallization (DRX) behavior of high-performance bridge steel plays an important role in guiding the thermomechanical processing process. In the present study, the hot deformation behavior of Q370qE bridge steel was investigated by hot compression tests conducted on a Gleeble 3800-GTC thermal-mechanical physical simulation system at temperatures ranging from 900 ℃ to 1100 ℃ and strain rates ranging from 0.01 s^(−1) to 10 s^(−1). The obtained results were used to plot the true stress-strain and work-hardening rate curves of the experimental steel, with the latter curves used to determine the critical strains for the initiation of DRX. The Zener-Hollomon equation was subsequently applied to establish the correspondence between temperature and strain rate during the high-temperature plastic deformation of bridge steel. In terms of the DRX volume fraction solution, a new method for establishing DRX volume fraction was proposed based on two theoretical models. The good weathering and corrosion resistance of bridge steel lead to difculties in microstructure etching. To solve this, the MTEX technology was used to further develop EBSD data to characterize the original microstructure of Q370qE bridge steel. This paper lays the theoretical foundation for studying the DRX behavior of Q370qE bridge steel. | Caiyi Liu Shicheng Liang Yan Peng Jianliang Sun Carlo Mapelli Silvia Barella Andrea Gruttadauria Marco Belf Ludovica Rovatti | 2023 | Chinese Journal of Mechanical Engineering2023，36，5： | 0 |
| 11 | Novel Gas-tight Multi-sampler for Discrete Deep-sea Water显示文摘The issues of how to quickly collect seawater samples and of how to make sure that those samples truly reflect the in-situ information on gas composition and concentration have therefore become a hot but difficult topic in the field of ocean technology.Most conventional seawater samplers only focus on collecting seawater itself,but take little consideration on gas preservation.A set of new oceanographic tools are presented for ocean resource exploration such as hydrothermal sulfide and gas hydrate,and for investigations on the processes and mechanisms of marine physical,chemical and biological evolutions.A gas-tight deep-sea water sampling system(GTWSS) is designed for the collection of deep-sea geochemical samples.This set of tools mainly consists of a conductivity temperature depth profiler(CTD),release devices and gas-tight deep-sea water samplers(GTWS).The GTWS is able to hold the gases in deep-sea water samples tightly,providing in-situ information on gas contents in the seawater samples and can be deployed on a routine wire-deployed CTD sampler for multi-layer discrete sampling of gas-tight seawater.Sea trials are performed successfully in 2008 and 2009,on a research vessel named HaiYang Si Hao in South China Sea,with the deepest trial depth 3 930 m.GTWSS is capable of quickly sampling 12 discrete gas-tight seawater samples(8.3 L per sample) during its single deployment.The head space method is employed to separate the gases from the seawater samples immediately after recovery of the seawater samples on the vessel.Field geochemical analysis is carried out by gaseous hydrocarbon sensors and an infrared gas analyzer.Results show that the concentrations of CH4 and CO2 in the seawater sampled by GTWSS are higher than those sampled by general non-gas-tight water samplers,thus confirming the gas tightness of GTWSS.Seawater samples can be collected quickly by using GTWSS,and GTWSS can keep the samples' integrity quite well. | HUANG Haocai YANG Canjun CHEN Shicheng CHEN Daohua SUN Chunyan NIU Wenda LI Fengbo LIU Guanghu CHEN Ying | 2012 | Chinese Journal of Mechanical Engineering2012，25，4： | 0 |
| 12 | Principal whitened gradient-projection algorithm for distribution control显示文摘In this paper,we use an information geometric algorithm to solve the distribution control problem.The new designed algorithm is called principal whitened gradient-projection algorithm.In the principal natural gradient step,we use principal whitened gradient algorithm to obtain an optimal trajectory of the weight vector on the B-spline manifold from the viewpoint of information geometry.In the projection step,we project the selected points on B onto M.The coordinates of the projections on M give the trajectory of the control input u. | ZHANG ShiCheng Sun HuaFei LI ChunHui | 2013 | Science China(Information Sciences)2013，56，3： | 0 |
| 13 | Shear behaviour of a rock bridge sandwiched between incipient joints under the influence of hydraulic pressures显示文摘The rock bridges sandwiched in incipiently jointed rock mass were considered as barriers that block the fluid seepage,and provide certain shear strength reservation.For better revealing the influence of hydraulic pressure on the failure behaviour of rock bridges,direct shear tests were carried out through a newly proposed method on rock samples that contain two parallel incipient joints.By developing the gypsum-silicone pad coupling samples,a conventional triaxial test system was qualified to implement direct shear tests with satisfied sealing capability.The results showed that the rock bridges could be failed through the tensile failure,shear failure and mixed failure mechanism.The hydraulic pressure would facilitate the tensile failure mechanism and induce rougher fracture surfaces;while the normal stress would facilitate the shear failure mechanism and induce less rough fracture.The hydraulic pressure reduced the global shear strength of the rock block through reducing the efficient normal stress applied on the rock bridge area,which was highly dependent on the joint persistence,k.Moreover,because of the iterating occurrence of the hydraulic pressure lag with the fracture propagation,the rock bridge failure stage in the shear stress-shear displacement curves displayed a fluctuation trend. | Shicheng Wang Xuxu Yang Lichao Li Panpan Sun Lei Yang Fuwei Li | 2023 | International Journal of Mining Science and Technology2023，33，2： | 0 |
| 14 | Detection of Multivariate Geochemical Anomalies Using the Bat-Optimized Isolation Forest and Bat-Optimized Elliptic Envelope Models显示文摘Isolation forest and elliptic envelope are used to detect geochemical anomalies,and the bat algorithm was adopted to optimize the parameters of the two models.The two bat-optimized models and their default-parameter counterparts were used to detect multivariate geochemical anomalies from the stream sediment survey data of 1:50000 scale collected from the Helong district,Jilin Province,China.Based on the data modeling results,the receiver operating characteristic(ROC)curve analysis was performed to evaluate the performance of the two bat-optimized models and their default-parameter counterparts.The results show that the bat algorithm can improve the performance of the two models by optimizing their parameters in geochemical anomaly detection.The optimal threshold determined by the Youden index was used to identify geochemical anomalies from the geochemical data points.Compared with the anomalies detected by the elliptic envelope models,the anomalies detected by the isolation forest models have higher spatial relationship with the mineral occurrences discovered in the study area.According to the results of this study and previous work,it can be inferred that the background population of the study area is complex,which is not suitable for the establishment of elliptic envelope model. | Yongliang Chen Shicheng Wang Qingying Zhao Guosheng Sun | 2021 | Journal of Earth Science2021，32，2： | 0 |
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