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| 1 | Mitochondrial localization of ORF346 causes pollen abortion in alloplasmic male sterility显示文摘The Nsa cytoplasmic male sterility(CMS) system confers stable male sterility and offers great potential for production of hybrid seeds in oilseed rape. However, genes responsible for male sterility in Nsa CMS have not been identified. By mitochondrial genome sequencing of Nsa CMS and its maintainer line,we identified in an Nsa CMS line several chimeric genes encoding hypothetical proteins harboring transmembrane domains. One novel chimeric gene orf346 showed high identity with cox1 at the 50 terminal region and was co-transcribed with nad3 and rps12 genes. Transgenic plants of orf346 fused with or without mitochondrial targeting peptide conferred complete male sterility in Arabidopsis. ORF346 was mitochondrion-localized. Expression of orf346 in Escherichia coli inhibited bacterial growth, with excessive accumulation of reactive oxygen species and decreased ATP content. These results reveal a link between the newly identified mitochondrial gene orf346 and the abortion of Nsa CMS. Inadequate energy supply and excessive accumulation of reactive oxygen species may account for pollen abortion in Nsa CMS plants. | Shifei Sang Hongtao Cheng Mengyu Hao Bingli Ding Desheng Mei Hui Wang Wenxiang Wang Jia Liu Li Fu Kede Liu Qiong Hu | 2021 | The Crop Journal2021，9，6： | 5 |
| 2 | Kinetics of oxidation of dimethyl trisulfide by potassium permanganate in drinking water显示文摘Metabolites of algae such as geosmin, 2- methylisoborneol etc. are reported to induce pungent odors into drinking water and attract additional scientifc attention. Recently, in China, taste and odor outbreaks in drinking water supply have become increasingly common. In source water affected by eutrophication, dimethyl trisulfide, speculated to be produced by decayed algae, was found to be the source of taste and odor issues and can be removed effectively by usual oxidation agents. In this experimental study, batch scale tests were carried out focusing on the removal of dimethyl trisulfide. Reaction kinetics of dimethyl trisulfide oxidized by potassium permanganate in water had been studied; influence factors such as pH, organic substrate, other existed taste, and odor contaminant in equivalent concentration were also dis- cussed. Results showed that dimethyl trisulfide can be removed by potassium permanganate efficiently; the ratio can reach more than 70% with oxidant dosage of 4 mg. L~ and contact time prolonged to 120 min. The dimethyl trisulfide decomposition followed a second-order kinetics pattern with a rate constant k -- 0.00213 L.(min.mg)-1. Typically, the degradation rate of dimethyl trisulfide was increased with the increasing KMnO4 dosage, but dramatically dropped with the increasing levels of humic acid (1.8-4.5 mg. L-1) and other odor-causing compounds (e.g. fl-cyclocitral, 0-1886.0 pg.L-1). Solution pH (5.2- 9.0) and initial dimethyl trisulfide concentration did not significantly affected the degradation. This study demon- strates that KMnO4 oxidation is an effective option to remove dimethyl trisulfide from water. | Xiaoyan MA Shifei HU Hongyu WANG Jun LI Jing HUANG Yun ZHANG Weigang LU Qingsong LI | 2012 | Frontiers of Environmental Science & Engineering2012，6，2： | 3 |
| 3 | Possibility of women treated with fertility-sparing surgery fornon-epithelial ovarian tumors to safely and successfully becomepregnant--a Chinese retrospective cohort study among 148cases显示文摘This study was performed to evaluate the oncological and reproductive outcomes of childbearing-agewomen treated with fertility-sparing surgery (FSS) for non-epithelial ovarian tumors in China. One hundred andforty eight non-epithelial ovarian tumor women treated with FSS between January I, 2000 and August 31, 2015from two medical centers in China were identified. Progression-free survival (PFS) was 88.5%, whereas overallsurvival (OS) was 93.9%. Univariate analysis suggested that delivery after treatment is related to PFS (P = 0.023),whereas histology significantly influenced OS. Cox regression analysis suggested that only histology was associatedwith PFS and OS (P 〈 0.05). Among the 129 women who completed adjuvant chemotherapy (ACT), nonedeveloped amenorrhea. Among the 44 women who desired pregnancy, 35 (79.5%) successfully had 51 gestationsincluding 35 live births without birth defects. Non-epithelial ovarian tumors can achieve fulfilling prognosis afterFSS and chemotherapy. Histology might be the only independent prognostic factor for PFS and OS. FSS followedby ACT appeared to have little or no effect on fertility. Meanwhile, postoperative pregnancy did not increase thePFS or OS. Use of gonadotropin-releasing hormone agonist was not beneficial for fertility. | Bin Yang Yan Yu Jing Chen Yan Zhang Ye Yin Nan Yu Ge Chen Shifei Zhu Haiyan Huang Yongqun Yuan Jihui Ai Xinyu Wang Kezhen Li | 2018 | Frontiers of Medicine2018，12，5： | 3 |
| 4 | Reconstruction clipping of ruptured anterior circulation aneurysms via supraorbital lateral keyhole approach显示文摘Background:Intracranial aneurysm(IA)is a serious disease.Analyze and review the cases of anterior circulation ruptured IA by supraorbital lateral keyhole approach,and summarize the experiences of this approach.Methods:Retrospective analysis of 16 cases of ruptured anterior circulation IA in our department from January 2019 to June 2020,CT angiography(CTA)was performed before operation.Analyzing the IA’s parameters by 3D-CT reconstruction.The IA was clipped by supraorbital lateral keyhole approach combined with the 3D-skull reconstruction.Extraventricular drainage was performed before craniotomy.Intraoperative neurophysiological monitoring was performed during the operation.After operation,fluorescein angiography and vascular ultrasound were performed to check the clipping effect.Intracranial pressure monitor was performed postoperatively.CTA was reexamined one week after operation.The modified Rankin Scale(MRS)was performed 6 months after operation.Results:There were 7 males(43.8%)and 9 females(56.2%),and the average age is 52.31±11.12 years old.Among them,11 patients(68.8%)were anterior communicating artery aneurysms and 5(31.2%)were middle cerebral artery aneurysms.All patients were out of hospital within 10 days without any death,without cerebral infarction,cerebrospinal fluid leakage and neurological impairments.About mRS score,after 6 months follow-up,8 cases(50%)had 0 point,4 cases(25%)had 1 point,and 4 cases(25%)had 2 points.Conclusions:For ruptured anterior circulation IA,the supraorbital lateral keyhole approach combined with ventricular drainage,intraoperative electrophysiological monitoring,and intraoperative vascular ultrasound is a safe and minimally invasive treatment.The application of reconstruction clipping can reconstruct the diameter of parent vessel and reduce the recurrence rate of IA. | Yuzhang Wu Yan Zhao Shengping Yu Fan Li Shifei Cai Chao Peng Zhen Wang Yifan Yang Bangyue Wang Xinyu Yang | 2022 | Chinese Neurosurgical Journal2022，8，2： | 2 |
| 5 | Full-Stokes polarization transformations and time sequence metasurface holographic display显示文摘With the development of micro/nano fabrication technology, metasurface holography has emerged as a revolutionary technology for the manipulation of light with excellent performance. However, for applications of full-Stokes polarization encryption and time sequence holographic display, multiplexing strategies of metasurfaces with large bandwidths and simple operations still need to be developed. As one of the most popular schemes of multiplexing, polarization multiplexed metasurfaces have shown flexible recording abilities for both free-space beam and surface waves. Here, by using a dielectric metasurface equipped with double phase holograms, we have achieved flexible polarization multiplexed transformations from one full-Stokes space to another. The vectorial hologram is optimized by a hybrid genetic algorithm and digitalized with subwavelength modulated units. Based on a quantitative map and remarkable information capacity, time sequence holographic display and complex optical encryption are experimentally demonstrated by changing input/output polarization channels in real time. We believe our method will facilitate applications in smart compact devices of dynamic display, dynamic optical manipulation,optical encryption, anticounterfeiting, etc. | Shifei Zhang Lingling Huang Guangzhou Geng Junjie Li Xiaowei Li Yongtian Wang | 2022 | Photonics Research2022，10，4： | 2 |
| 6 | Preparation and electrochemical properties of Ca-doped Li 4 Ti 5 O 12 as anode materials in lithium-ion battery显示文摘 | Qianyu Zhang Chengli Zhang Bo Li Shifei Kang Xi Li Yangang Wang | 2013 | Electrochimica Acta2013，，： | 1 |
| 7 | An optimizing method of RBF neural network based on genetic algorithm 显示文摘 | DING Shifei XU LI SU Chunyang | 2012 | Neural Computing & Applications2012，21，2： | 1 |
| 8 | Intelligent Passive Detection of Aerial Target in Space-Air-Ground Integrated Networks显示文摘Passive detection of moving target is an important part of intelligent surveillance. Satellite has the potential to play a key role in many applications of space-air-ground integrated networks(SAGIN). In this paper, we propose a novel intelligent passive detection method for aerial target based on reservoir computing networks. Specifically, delayed feedback networks are utilized to refine the direct signals from the satellite in the reference channels. In addition, the satellite direct wave interference in the monitoring channels adopts adaptive interference suppression using the minimum mean square error filter. Furthermore, we employ decoupling echo state networks to predict the clutter interference in the monitoring channels and construct the detection statistics accordingly. Finally, a multilayer perceptron is adopted to detect the echo signal after interference suppression. Extensive simulations is conducted to evaluate the performance of our proposed method. Results show that the detection probability is almost 100% when the signal-to-interference ratio of echo signal is-36 dB, which demonstrates that our proposed method achieves efficient passive detection for aerial targets in typical SAGIN scenarios. | Mingqian Liu Chunheng Liu Ming Li Yunfei Chen Shifei Zheng Nan Zhao | 2022 | China Communications2022，19，1： | 1 |
| 9 | Research and pro- gress of cluster algorithms based on granular computing显示文摘 | Ding Shifei Xu Li Zhu Hong Zhang Liwen | 2010 | Inter- national Journal of Digital Content Technology and its Applications2010，4，5： | 1 |
| 10 | Evolutionary artificial neural networks: a review显示文摘 | Shifei Ding Hui Li Chunyang Su Junzhao Yu Fengxiang Jin | 2013 | Artificial Intelligence Review2013，，： | 1 |
| 11 | A parallel attribute reduction algorithm based on Affinity Propagation clustering显示文摘 | Hong Zhu Shifei Ding Xinzheng Xu Li Xu | 2013 | Journal of Computers2013，，4： | 1 |
| 12 | New loci for refractive errors and ocular biometric parameters in young Chinese Han adults显示文摘Myopia has become a major public health issue with an increasing prevalence.There are still individuals who experience similar environmental risk factors and,yet,remain non-myopic.Thus,there might be genetic factors protecting people from myopia.Considering the opposite ocular characteristics of primary angle closure glaucoma(PACG)to myopia and possible common pathway between them,we propose that certain risk genes for PACG might act as a protective factor for myopia.In this study,2,678 young adults were genotyped for 37 targeted single nucleotide polymorphisms.Compared with emmetropia,rs1401999(allele C:OR=0.795,P=0.03;genotype in dominant model:OR=0.759,P=0.02)and rs1258267(allele A:OR=0.824,P=0.03;genotype in dominant model:OR=0.603,P=0.01)were associated with low to moderate myopia and high myopia,respectively.Genotype under recessive model of rs11024102 was correlated with myopia(OR=1.456,P=0.01),low to moderate myopia(OR=1.443,P=0.02)and high myopia(OR=1.453,P=0.02).However,these associations did not survive Bonferroni correction.Moreover,rs1401999,rs1258267,and rs11024102 showed associations with certain ocular biometric parameters in different groups.Our study suggests that ABCC5,CHAT and PLEKHA7 might be associated with refractive errors by contributing to the regulation of ocular biometry,in terms of uncorrected results and their biological functions. | Yunyun Sun Zi-Bing Jin Shifei Wei Hongyan Jia Kai Cao Jianping Hu Caixia Lin Wenzai An Jiyuan Guo He LiJing Fu Shi-Ming Li Ningli Wang the Anyang University Students Eye Study Group the Anyang University Students Eye Study Group | 2022 | Science China(Life Sciences)2022，65，10： | 1 |
| 13 | An optimizing method of RBF neural network based on genetic algorithm 显示文摘 | DING Shifei XU Li SU Chunyang | 2012 | Neural Comput & Applic2012，21，2： | 1 |
| 14 | Research and Progress of Cluster Algorithms based on Granular Computing 显示文摘 | Ding Shifei Xu Li Zhu Hong | 2010 | International Journal of Digital Content Technology and its Applications2010，4，5： | 1 |
| 15 | An optimizing method of RBF neural network based on genetic algorithm显示文摘 | Shifei Ding Li Xu Chunyang Su Fengxiang Jin | 2012 | Neural Computing and Applications2012，，2： | 1 |
| 16 | Research and progress of cluster algorithms based on granular computing显示文摘 | Ding Shifei Xu Li Zhu Hong | 2010 | International Journal of Digital Content Technology and its Applications2010，4，5： | 1 |
| 17 | Anthraquinones and Lignans from Cassia occidentalis 显示文摘 | Li Shifei Di Yingtong Wang Yuehu | 2010 | Helvetica Chimica Acta2010，93，： | 1 |
| 18 | Complete organelle genomes of Sinapis arvensis and their evolutionary implications显示文摘Sinapis arvensis,belonging to the genus Sinapis of the family Brassicaceae,has good agronomic characters that make it a valuable genetic resource for crop improvement and is a cytoplasmic source of heterologous cytoplasmic male sterility(CMS).In addition,S.arvensis has played an important role in the evolution of the six major cultivated Brassica species involved in the triangle of U.Using next-generation sequencing,we assembled and revealed the gene composition of S.arvensis cytoplasmic genome.The chloroplast genome comprises 153,590 bp,with 112 individual genes,including 4 r RNA,29 t RNA,and 79 proteincoding genes.The mitochondrial genome comprises 240,024 bp,with 54 genes,including 18 t RNA,three r RNA and 33 protein-coding genes.Genome structure and evolutionary analysis indicated that the sequences of the S.arvensis organellar genomes were more similar to those of Brassica nigra and B.carinata than to those of other Brassicaceae species.Four mitochondrial open reading frames displaying chimeric structural features and encoding hypothetical proteins with transmembrane domains may account for the infertility of Nsa CMS previously derived from somatic cell hybridization between B.napus and S.arvensis.These results will not only contribute to utilize the germplasm resource of S.arvensis,and comprehend the evolution of organelle genomes within the Brassicaceae family,but also help to identify genes conditioning the alloplasmic male sterility of Nsa CMS in B.napus. | Shifei Sang Hongtao Cheng Desheng Mei Li Fu Hui Wang Jia Liu Wenxiang Wang Qamar U.Zaman Kede Liu Qiong Hu | 2020 | The Crop Journal2020，8，4： | 1 |
| 19 | Computational Tools and Resources for CRISPR/Cas Genome Editing显示文摘The past decade has witnessed a rapid evolution in identifying more versatile clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein(Cas)nucleases and their functional variants,as well as in developing precise CRISPR/Cas-derived genome editors.The programmable and robust features of the genome editors provide an effective RNAguided platform for fundamental life science research and subsequent applications in diverse scenarios,including biomedical innovation and targeted crop improvement.One of the most essential principles is to guide alterations in genomic sequences or genes in the intended manner without undesired off-target impacts,which strongly depends on the efficiency and specificity of single guide RNA(sgRNA)-directed recognition of targeted DNA sequences.Recent advances in empirical scoring algorithms and machine learning models have facilitated sgRNA design and off-target prediction.In this review,we first briefly introduce the different features of CRISPR/Cas tools that should be taken into consideration to achieve specific purposes.Secondly,we focus on the computer-assisted tools and resources that are widely used in designing sgRNAs and analyzing CRISPR/Cas-induced on-and off-target mutations.Thirdly,we provide insights into the limitations of available computational tools that would help researchers of this field for further optimization.Lastly,we suggest a simple but effective workflow for choosing and applying web-based resources and tools for CRISPR/Cas genome editing. | Chao Li Wen Chu Rafaqat Ali Gill Shifei Sang Yuqin Shi Xuezhi Hu Yuting Yang Qamar U.Zaman Baohong Zhang | 2023 | Genomics, Proteomics & Bioinformatics2023，21，1： | 0 |
| 20 | Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice显示文摘Comparative gene identification-58(CGI-58),also known asα/βhydrolase domain containing 5,is the co-activator of adipose triglyceride lipase that hydrolyzes triglycerides stored in the cytosolic lipid droplets.Mutations in CGI-58 gene cause Chanarin-Dorfman syndrome(CDS),an autosomal recessive neutral lipid storage disease with ichthyosis.The liver pathology of CDS manifests as steatosis and steatohepatitis,which currently has no effective treatments.Perilipin-3(Plin3)is a member of the Perilipin-ADRP-TIP47 protein family that is essential for lipid droplet biogenesis.The objective of this study was to test a hypothesis that deletion of a major lipid droplet protein alleviates fatty liver pathogenesis caused by CGI-58 deficiency in hepatocytes.Adult CGI-58-floxed mice were injected with adeno-associated vectors simultaneously expressing the Cre recombinase and microRNA against Plin3 under the control of a hepatocyte-specific promoter,followed by high-fat diet feeding for 6 weeks.Liver and blood samples were then collected from these animals for histological and biochemical analysis.Plin3 knockdown in hepatocytes prevented steatosis,steatohepatitis,and necroptosis caused by hepatocyte CGI-58 deficiency.Our work is the first to show that inhibiting Plin3 in hepatocytes is sufficient to mitigate hepatocyte CGI-58 deficiency-induced hepatic steatosis and steatohepatitis in mice. | Xinyu Bao Xiaogen Ma Rongfeng Huang Jianghui Chen Haoran Xin Meiyu Zhou Lihua Li Shifei Tong Qian Zhang Guanghou Shui Fang Deng Liqing Yu Min-Dian Li Zhihui Zhang | 2022 | Journal of Molecular Cell Biology2022，14，8： | 0 |
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