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| 1 | p53 upregulated by HIF-la promotes hypoxiainduced G2/M arrest and renal fibrosis in vitro and in vivo显示文摘Hypoxia plays an important role in the genesis and progression of renal fibrosis.The underlying mechanisms, however, have not been sufficiently elucidated. We examined the role of p53 in hypoxia-induced renal fibrosis in cell culture (human and rat renal tubular epithelial cells) and a mouse unilateral ureteral obstruction (UUO) model. Cell cycle of tubular cells was determined by flow cytometry, and the expression of profibrogenic factors was determined by RT-PCR, immunohistochemistry, and western blotting. Chromatin immunoprecipitation and luciferase reporter experiments were performed to explore the effect of HIF-lα on p53 expression. We showed that, in hypoxic tubular cells, p53 upregulation suppressed the expression of CDK1 and cyclins Bl and DI, leading to cell cycle (G2/M) arrest (or delay) and higher expression of TGF-β, CTGF, collagens, and fibronectin. p53 suppression by siRNA or by a specific p53 inhibitor (PIF-α) triggered opposite effects preventing the G2/M arrest and profibrotic changes. In vivo experiments in the UUO model revealed similar antifibrotic results following intraperitoneal administration of PIF-α(2.2 mg/kg). Using gain-of-function, loss-of-function, and luciferase assays, we further identified an HRE3 region on the p53 promoter as the HIF-lα-binding site. The HIF-la-HRE3 binding resulted in a sharp transcriptional activation of p53. Collectively, we show the presence of a hypoxia-activated, p53-responsive profibrogenic pathway in the kidney. During hypoxia, p53 upregulation induced by HIF-la suppresses cell cycle progression, leading to the accumulation of G2/M cells, and activates profibrotic TGF-β and CTGF-mediated signaling pathways, causing extracellular matrix production and renal fibrosis. | Limin Liu Peng Zhang Ming Bai Lijie He Lei Zhang Ting Liu Zhen Yang Menglu Duan Minna Liu Baojian Liu Rui Du Qi Qian Shiren Sun | 2019 | Journal of Molecular Cell Biology2019，11，5： | 18 |
| 2 | Vegetation and Environment History for the Past 14 000 yr BP from Dingnan, Jiangxi Province, South China显示文摘迟了的 Pleistocene-Holocene 花粉,磷,和炭记录在南部的中国在南部的江西省从 peatland 被重建。今天的区域与米饭稻,蔬菜,和水果统治的村庄,小镇,和农业有一处多山、滚动的风景第二等的森林和松重新植林的花园,以及区域。记录在 14 300 年 BP 前开,与统治沼泽地区域的 Alnus 树林并且与包围斜坡上的开的 Quercus 树林。森林区域从约 12 800 年 BP 变得更复杂并且从 9 000 年 BP 进一步。在约 6 000 年 BP,有变清的证据,由 45004000 年 BP,在沼泽地 Alnus 和陆上的森林的完全的倒塌作为低区域被变换成米饭生产。为许多记录,在这个地点附近的火的出现是低的,尽管有地区性的火的证据。火在变清被用作一个工具然后在残株在米饭收获以后烧的年度周期使用了。营养素铺平,在沉积由全部的磷思考了似乎是仔细,与在表面的森林变化和高价值有关,层可能源于与农业联系的陆地管理技术。因此,人的影响极大地改变了森林盖子,火频率,和滋养的动力学;这为约 6 000 年 BP 是明显的然后加强向现代。 | John Richard Dodson Shirene Hickson Rachel Khoo Xiao-Qiang Li Jemina Toia Wei-Jian Zhou | 2006 | Journal of Integrative Plant Biology2006，48，9： | 7 |
| 3 | Expression, purification, and functional characterization of recombinant PTD-SARA显示文摘为蛋白质是的受体激活(SARA ) 的 Smad 锚为在支持发信号的小径的 fibrotic 起一个重要作用的 Smad2/3 的一个有约束力的搭挡由转变生长 factor-1 (TGF-1 ) 开始了。C 终端 SARA 的 665750 aa 包括 Smad 有约束力的领域(SBD ) 。由通过 SBD 的直接相互作用, SARA 禁止 Smad2/3 phosphorylation 并且堵住在 Smad2/3 和 Smad4 之间的相互作用,从而制止纤维变性的过程。在这研究,我们基于 SBD 顺序构造了 SARA 肽 aptamer。recombinant SARA aptamer,与一个蛋白质 transduction 领域(PTD-SARA ) 熔化了,被克隆,从 E 净化了。coli,并且第一次描绘了。编码顺序的全身的 PTD-SARA,与 E 创造了。coli 赞成了 codons,被克隆进 pQE-30 向量,并且 recombinant plasmid 被转变成 M15 紧张。在 Isopropyl -D-1-Thiogalactopyranoside (IPTG ) 以后正式就职和 Ni2+ 亲密关系纯化, recombinant PTD-SARA 被定序的 immunoblotting 和蛋白质 N 终端进一步识别。Epifluorescence 显微镜学表明 recombinant PTD-SARA 比 SARA 更高效地被变成细胞质和原子核。而且, recombinant PTD-SARA 被发现 E-cadherin 和下面调整的水平到起来调整 -SMA 和 phospho-Smad3 的层次更高效地比 SARA (P < 0.05 ) 。我们的工作探索了一个方法获得 recombinant PTD-SARA 蛋白质。recombinant PTD-SARA 熔化蛋白质能进入 HK2 房间(使不朽的近似小管上皮的房间线) 更高效地比 SARA 蛋白质和颠倒被 TGF-1 比 SARA 蛋白质更有效地导致的肾的 epithelial-to-mesenchymal transdifferentiation 过程。Recombinant PDT-SARA 是可能的是肾的纤维变性的临床的预防和处理的一个潜在的候选人。 | Chen Huang Rui Du Peng Zhang Hua Meng Huiwei Jia Yang Song Man Li Yingqi Zhang Shiren Sun | 2011 | Acta Biochimica et Biophysica Sinica2011，43，2： | 4 |
| 4 | Frictional Behavior of a Micro-sized Superconducting Fiber in a Low-Temperature Medium: Experimental and Computational Analysis显示文摘 | Shiren La Jun Wang Xingyi Zhang Youhe Zhou | 2018 | Acta Mechanica Solida Sinica2018，31，4： | 3 |
| 5 | Cgr1,a ripe rot resistance QTL in Vitis amurensis‘Shuang Hong’grapevine显示文摘Ripe rot is a serious grapevine disease in Vitis L.and Muscadinia(Planch.)Small.However,resistance to this disease has been reported in some oriental Vitis species.To identify resistance-related Quantitative Trait Loci(QTLs)from the Chinese grape species V.amurensis,an F1 population of V.vinifera‘Cabernet Sauvignon’×V.amurensis‘Shuang Hong’was used to map the ripe rot resistance loci expected in‘Shuang Hong’grape.A total of 7598 single nucleotide polymorphisms(SNPs)between the parents were identified in our previous study,and 934 SNPs were selected for genetic map construction.These SNPs are distributed across the 19 chromosomes covering a total of 1665.31 cM in length,with an average of 1.81 cM between markers.Ripe rot resistance phenotypes among the hybrids were evaluated in vitro using excised leaves for three consecutive years from 2016 to 2018;a continuous variation was found among the F1 hybrids,and the Pearson correlation coefficients of the phenotypes scored in all three years were significant at the 0.01 level.Notably,the first QTL reported for resistance to grape ripe rot disease,named Cgr1,was identified on chromosome 14 of‘Shuang Hong’grapevine.Cgr1 could explain up to 19.90%of the phenotypic variance.In addition,a SNP named‘np19345’was identified as a molecular marker closely linked to the peak of Cgr1 and has the potential to be developed as a marker for the Cgr1 resistance haplotype. | Peining Fu Quanyou Tian Gongti Lai Rongfang Li Shiren Song Jiang Lu | 2019 | Horticulture Research2019，6，1： | 3 |
| 6 | Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma显示文摘 | Shiren Sun Xiaoxuan Ning Jie Liu Lili Liu Yu Chen Shuang Han Yanqi Zhang Jie Liang Kaichun Wu Daiming Fan | 2007 | Biochemical and Biophysical Research Communications2007，，4： | 2 |
| 7 | A Hierarchical Coordinate System for Geoprocessing and Cartography 显示文摘 | GOODCHILD M F SHIREN Y | 1992 | CVGIP: Graph Models Image Process1992，54，1： | 1 |
| 8 | A Hierarchical Spatial Data Structure for Global Geographic Information Systems 显示文摘 | GOODCHILD M F SHIREN Y | 2000 | CVGIP: Graphical Models and Image Processing2000，54，1： | 1 |
| 9 | SB- DART: A research and teaching software tool for Plane-Parallel radiative transfer in the Earth's at- mosphere 显示文摘 | Ricchiazzi P Yang Shiren Gautier C | 1998 | Bulletin of the American Meteoro- logical Society1998，79，10： | 1 |
| 10 | Development and test of an error model for categorical data显示文摘 | Michael F. Goodchild Sun Guoqing Yang Shiren | 1992 | International Journal of Geographical Information Science1992，，2： | 1 |
| 11 | A hierarchical data structure for globac information sustems 显示文摘 | Goodchild M F Yang Shiren | 1992 | Computer Vision and Geographic Image Processing1992，54，1： | 1 |
| 12 | Epoxide-Terminated Carbon Nanotubes 显示文摘 | Wang Shiren Liang Richard Wang Ben | 2007 | Carbon2007，45，15： | 1 |
| 13 | A Hierarchical Spatial Data Structure for Global Geographic Information Systems显示文摘 | Goodchild M F Shiren Y | 1992 | Graphical Models and Image Processing1992，54，1： | 1 |
| 14 | A Hierarchical Data Structure for Global Geographic Information Systems 显示文摘 | Goodchild M F Yang Shiren | 1992 | Computer Vision and Geographic Image Processing1992，54，1： | 1 |
| 15 | SBDART: A research and teaching software tool for plane-parallel radiative transfer in the earth's at mosphere显示文摘 | Paul Ricchiazzi Shiren Yang | 1998 | Bull Amer Meteor Soc1998，79，： | 1 |
| 16 | Enhancing thermal conductivity of glass fiber/polymer composites through carbon nanotubes incorporation显示文摘 | Shiren Wang Jingjing Qiu | 2010 | Composites Part B2010，，7： | 1 |
| 17 | Hypoxia-inducible factor-lalpha induces Twist expression in tubular epithelial cells subjected to hypoxia, leading to epithelial- to-mesenchymal transition 显示文摘 | Sun Shiren Ning Xiaoxuan Zhang Yanqi | 2009 | Kidney Int2009，75，： | 1 |
| 18 | A Hierarchical Data Structure for Global Geographic Information Systems显示文摘 | Goodchild M F Yang Shiren | 1992 | Computer Vision and Geographic Image Processing1992，54，1： | 1 |
| 19 | Accumulation of tissue advanced glycation end products correlated with glucose exposure dose and associated with cardiovascular morbidity in patients on peritoneal dialysis显示文摘 | Jianping Jiang Pingyan Chen Jianghua Chen Xueqing Yu Di Xie Changlin Mei Fei Xiong Wei Shi Wei Zhou Xusheng Liu Shiren Sun Ping Zhang Xiao Yang Yixiang Zhang Yanmin Zhang Xinling Liang Zhimin Zhang Qizhan Lin Yan Yu Toshio Miyata Jianwei Tian Min Liang We | 2012 | Atherosclerosis2012，，1： | 1 |
| 20 | A Hierarchical Data Structure for Global Geographic Information Systems显示文摘 | GOODCHILD M F SHIREN Y | 1992 | Computer Vision and Geographic Image Processing1992，54，1： | 1 |
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