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| 1 | Construction of infectious cDNA clones of PRRSV:Separation of coding regions for nonstructural and structural proteins显示文摘Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the causative agent of the ongoing 'porcine high fever syndrome' in China, is capable of genetic and antigenic mutations at high fre- quency. How to design vaccine rationally to keep up with the ever-changing prevalent PRRSV variant is of great interest. We developed an infectious cDNA clone of an attenuated strain of Type II PRRSV, and further manipulated the infectious cDNA clone by inserting polylinker between ORF1 and ORF2, en- coding for nonstructural- or structural-protein, respectively. The cDNA was generated from the cell-attenuated virus strain, APRRS, via RT-PCR, and followed by nucleotide sequencing and molecular cloning. The full-length of the APRRS genomic RNA was determined as 15521 nucleotides in length excluding poly(A) tail, which has a 99.7% nucleotide identity with that of PRRSV Nsp strain, also a vac- cine strain. Based on the nucleotide sequencing results, the full-length cDNA clone was assembled in pBlueScript vector, under the control of T7 promoter at the immediate 5′ terminus of genome. To dis- cern the rescued viruses from that of parental virus, a Mlu I restriction site was engineered into ORF5 coding region. Upon transfection of the in vitro transcripts of both the original and Mlu I-tagged cDNAs into MA-104 cells, typical PRRSV cytopathic effects were observed. The rescued viruses from the full-length cDNA clones displayed the same virological and molecular properties. Subsequently, PCR-based mutagenesis was conducted to separate the coding regions between PRRSV nonstructural genes, ORF1, and structural proteins, ORF2-ORF7. The synthetic RNA of such mutant clone, pCSA, was infectious and the rescued virus shared similar properties with that of the parental virus. This study provided a valuable tool for development of chimeric PRRSV as vaccine candidate offering cross-protection to various genetically diversified PRRSV strains, and a platform for further develop- ment of PRRSV as a gene expression vector for recombinant vaccines against other significant swine diseases. | YUAN ShiShan & WEI ZuZhang Department of Animal Infectious Diseases, Key Laboratory of Animal Parasitology Chinese Ministry of Agriculture, Shanghai Vet- erinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200232, China | 2008 | Science China(Life Sciences)2008，51，3： | 19 |
| 2 | Mutational analysis of the SDD sequence motif of a PRRSV RNA-dependent RNA polymerase显示文摘The subgenomic mRNA transcription and genomic replication of the porcine reproductive and respiratory syndrome virus (PRRSV) are directed by the viral replicase. The replicase is expressed in the form of two polyproteins and is subsequently processed into smaller nonstructural proteins (nsps). nsp9, containing the viral replicase, has characteristic sequence motifs conserved among the RNA-dependent RNA polymerases (RdRp) of positive-strand (PS) RNA viruses. To test whether the conserved SDD motif can tolerate other conserved motifs of RNA viruses and the influence of every residue on RdRp catalytic activity, many amino acids substitutions were introduced into it. Only one nsp9 substitution, of serine by glycine (S3050G), could rescue mutant viruses. The rescued virus was genetically stable. Alteration of either aspartate residue was not tolerated, destroyed the polymerase activity, and abolished virus transcription, but did not eliminate virus replication. We also found that the SDD motif was essentially invariant for the signature sequence of PRRSV RdRp. It could not accommodate other conserved motifs found in other RNA viral polymerases, except the GDD motif, which is conserved in all the other PS RNA viruses. These findings indicated that nidoviruses are evolutionarily related to other PS RNA viruses. Our studies support the idea that the two aspartate residues of the SDD motif are critical and essential for PRRSV transcription and represent a sequence variant of the GDD motif in PS RNA viruses. | ZHOU Yan ZHENG HaiHong GAO Fei TIAN DeBin YUAN ShiShan | 2011 | Science China(Life Sciences)2011，54，9： | 15 |
| 3 | Overexpression of Annexin A2 Is Associated with Abnormal Ubiquitination in Breast Cancer显示文摘Abnormal expression of annexin A2 contributes to metastasis and infiltration of cancer cells.To elucidate the cause of abnormal expression of annexin A2,Western blotting,immunoproteomics and immunohistochemical staining were performed to analyze differentially ubiquitinated proteins between fresh breast cancer tissue and its adjacent normal breast tissue from five female volunteers.We detected an ubiquitinated protein that was up-regulated in the cancer tissue,which was further identified as annexin A2 by mass spectrometry.These results suggest that abnormal ubiquitination and/or degradation of annexin A2 may lead to presence of annexin A2 at high level,which may further promote metastasis and infiltration of the breast cancer cells. | Shishan Deng Baoqian Jing Tianyong Xing Lingmi Hou Zhengwei Yang | 2012 | Genomics, Proteomics & Bioinformatics2012，10，3： | 2 |
| 4 | Perovskite-type B-site Bi-doped ceramic membranes for oxygen separation显示文摘Novel mixed conducting oxides, B-site Bi-doped perovskites were exploited and synthesized. Cubic perovskite structures were formed for BaBi0.2COyFe0.8-yO3-δ (y≤0.4) and BaBixCo0.2Fe0.8-xO3-δ (x=0.1 -0.5). The materials exhibited considerable high oxygen permeability at high temperature. The oxygen permeation flux of BaBi0.2Co0.35Fe0.45O3-δmembrane reached about0.77×106 mol/cm2 ·s under an air/helium oxygen partial pressure gradient at 900 ℃, which was much higher than that of other bismuth-contained mixed conducting membranes. The permeation fluxes of the materials increased with the increase of cobalt content, but no apparent simple relationship was found with the bismuth content. The materials also demonstrated excellent reversibility of oxygen adsorption and desorption. Stable time-related oxygen permeation fluxes were found for BaBi0.2Co0. | Zongping Shao You Cong Guoxing Xiong Shishan Sheng Weishen Yang | 2000 | Chinese Science Bulletin2000，45，10： | 2 |
| 5 | New Occurrence of the Early Cambrian Chengjiang Fauna in Haikou, Kunming, Yunnan Province,and Study on Trilobitoidea显示文摘This paper gives a brief report on the new occurrence of the Early CambrianQiongzhusi’ an Chengjiang fauna in the Haikou area, Kunming and its assemblage feature andthe stratigraphic section of the horizon yielding these fossils. Eight genera and 8 species ofTrilobitoidea are described here, embracing Leanchoilia asiatica Luo et Hu (sp. nov.), Yohoiasinensis Luo et Hu (sp. nov.), Zhongxinia speciosa Luo et Hu (gen. et sp. nov.), Xandarellaspectuculum Hou et al., Dianchia mirabilis Luo et Hu (gen. et sp. nov.), Kuamaia lata Hou,Retifacies longispinus Luo et Hu (sp. nov.) and Sinoburius lunaris Hou et al. | Luo Huilin Hu Shixue Zhang Shishan and Tao Yonghe Yunnan Institute of Geological Sciences, Kunming Yunnan Kunyang Phosphorite Mine, Jinning, Yunnan | 1997 | Acta Geologica Sinica(English Edition)1997，71，2： | 2 |
| 6 | Effect of Supports on the Gold Catalyst Activity for Catalytic Combustion of CO and HCHO显示文摘 | Meilin Jia Yuenian Shen Changyan Li Zhaorigetu Bao Shishan Sheng | 2005 | Catalysis Letters (-)2005，，3： | 1 |
| 7 | Discrimination of viable Vibrio vulnificus cells from dead cells in real-time PCR显示文摘 | WANG Shishan LEVIN R E | 2006 | Journal of Microbiological Methods2006，64，5： | 1 |
| 8 | Increased calcium deposits and decreased Ca 2+ -ATPase in erythrocytes of ascitic broiler chickens显示文摘 | Kai Li Lihong Zhao Guangrui Geng Liqin Ma Shishan Dong Tong Xu Jianlin Wang Huiyu Wang Yong Tian Jian Qiao | 2010 | Research in Veterinary Science2010，，3： | 1 |
| 9 | Overex- pression of Annexin A2 Is Associated with Abnormal Ubiquit- ination in Breast Caneer显示文摘 | Shishan Deng Baoqian Jing Tianyong Xing | | Genomies Proteomies Bioinforma0，，： | 1 |
| 10 | Ultrasensitive dopamine sensor based on novel molecularly imprinted polypyrrole coated carbon nanotubes显示文摘 | Tao Qian Chenfei Yu Xi Zhou Peipei Ma Shishan Wu Lina Xu Jian Shen | 2014 | Biosensors and Bioelectronics2014，，： | 1 |
| 11 | Polymer/silica nanocomposites: preparation, characterization, properties, and applications显示文摘 | Zou Hua Wu Shishan Shen Jian | 2008 | Chemical Reviews2008，108，： | 1 |
| 12 | Preparation and characterization of nylon 66/montmorillonite nanocomposites with co-treated montmorillonites显示文摘 | Bing Han Gending Ji Shishan Wu | 2003 | European Polymer Journal2003，39，： | 1 |
| 13 | Preparation of SiO2 polymeric sols for nanoporous ceramic membranes by polymeric sol-gel method显示文摘 | BAO Yun ZHAO Honghin SHENG Shishan | 1997 | Chinese Journal of Catalysis1997，11，2： | 1 |
| 14 | Studies on structure and properties of HDPE functionalized through ultraviolet irradia- tion and its blends with CaCO3 显示文摘 | Wu Shishan Ran Qianping Liu Zhimin | 2005 | Polymer - Plastics Technolo- gy and Engineering2005，44，89： | 1 |
| 15 | Polymer/silica nanocom-posites: Preparation, characterization, properties, and applications显示文摘 | Zou Hua Wu Shishan Shen Jian | 2008 | Chern Rev2008，108，9： | 1 |
| 16 | Polymer/silica nanocompos- ites: Preparation, characterization, properties, and applica- tions显示文摘 | Zou Hua Wu Shishan Shen Jian | 2008 | Chem Rev2008，108，9： | 1 |
| 17 | A Simple and low-cost method for the preparation of monodisperse hollow silica spheres 显示文摘 | Zou Hua Wu Shishan Ran Qianping | 2008 | J Phys Chem C2008，112，11： | 1 |
| 18 | Pt supported on Zn modified silicalite-1 zeolite as a catalyst for n-hexane aromatization显示文摘Platinum(Pt)supported on Zinc(Zn)modified silicalite-1(S-1)zeolite(denoted as Pt-Zn/S-1)was prepared by using a wetness-impregnation method and applied in the n-hexane aromatization reaction for the first time.Both Lewis and Bronsted acid sites were detected in Pt-Zn/S-1 catalyst by means of FT-IR adsorption of NH3 experiment,which were identified as mostly weak and medium ones.Besides,Pt and Zn species showed strong interaction,as revealed by the TPR(Temperature-programmed reduction)and XPS(X-ray photoelectron spectroscopy)experiments.Pt-Zn/S-1 catalyst exhibited excellent aromatization function rather than isomerization and cracking side reactions in the conversion of n-hexane.Pulse experimental study showed that 75.6%of n-hexane conversion and 76.8%of benzene selectivity were obtained over Pt0.1-Zn60/S-l catalyst at 550℃ and under atmospheric pressure.By spectroscopy tests and pulse experimental results,it was concluded that the n-hexane aromatization over Pt-Zn/S-1 catalyst follows a metal-acid bifunctional mechanism.Furthermore,with the assistance of Zn,the electron-deficient Pt species in Pt-Zn/S-1 showed good sulfur tolerance performance. | Guodong Liu Jiaxu Liu Ning He Shishan Sheng Guiru Wang Hongchen Guo | 2019 | Journal of Energy Chemistry2019，28，7： | 1 |
| 19 | The structure and properties of PA 6/MMT nanocomposites prepared by melt compounding 显示文摘 | Shishan W Dingjun J Xiaodong O | 2004 | Polym Eng Sci2004，44，11： | 1 |
| 20 | Study on the Problems and Countermeasure in the Animal Products Quality and Safety显示文摘As the supply chain of animal products is long,there are many factors which would influence the quality safety of animal production.Therefore,by focusing on the present and having a vision for future,Jiaozuo government promulgated Ten Polices on the Food Safety in Jiaozuo City,which pushed forward the technological development,intensified measures to monitor the quality of animal products,set up a series of mechanism,and provided reference for the food quality and safety monitoring. | Shishan WANG | 2013 | Asian Agricultural Research2013，5，9： | 1 |
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