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| 1 | A Strigolactone Biosynthesis Gene Contributed to the Green Revolution in Rice显示文摘Plant architecture is a complex agronomic trait and a major factor of crop yield,which is affected by several important hormones.Strigolactones(SLs)are identified as a new class hormoneinhibiting branching in many plant species and have been shown to be involved in various developmental processes.Genetical and chemical modulation of the SL pathway is recognized as a promising approach to modify plant architecture.However,whether and how the genes involved in the SL pathway could be utilized in breeding still remain elusive.Here,we demonstrate that a partial loss-of-function allele of the SL biosynthesis gene,HIGH TILLERING AND DWARF 1/DWARF17(HTD1/D17),which encodes CAROTENOID CLEAVAGE DIOXYGENASE 7(CCD7),increases tiller number and improves grain yield in rice.We found that the HTD1 gene had been widely utilized and co-selected with Semidwarf 1(SD1),both contributing to the improvement of plant architecture in modern rice varieties since the Green Revolution in the 1960s.Understanding how phytohormone pathway genes regulate plant architecture and how they have been utilized and selected in breeding will lay the foundation for developing the rational approaches toward improving crop yield. | Yuexing Wang Lianguang Shang Hong Yu Longjun Zeng Jiang Hu Shen Ni Yuchun Rao Sanfeng Li Jinfang Chu Xiangbing Meng Lei Wang Ping Hu Jijun Yan Shujing Kang Minghao Qu Hai Lin Tao Wang Quan Wang Xingming Hu Hongqi Chen Bing Wang Zhenyu Gao Longbiao Guo Dali Zeng Xudong Zhu Guosheng Xiong Jiayang Li Qian Qian | 2020 | Molecular Plant2020，13，6： | 23 |
| 2 | MdGSTF6,activated by MdMYB1,plays an essential role in anthocyanin accumulation in apple显示文摘Anthocyanins are biosynthesized on the cytosolic surface of the endoplasmic reticulum and then transported into the vacuole for storage.Glutathione S-transferases(GSTs)are considered to be responsible for the transport of anthocyanins into the vacuole.However,the regulatory mechanisms of GSTs in plants are still unclear.Here,we performed a genome-wide analysis and identified 69 GST genes in apple.The expression of MdGSTF6 was positively correlated with the anthocyanin content(r=0.949)during‘Yanfu 8’fruit development.The overexpression of MdGSTF6 in the Arabidopsis thaliana tt19 mutant resulted in seedlings of 35S::MdGSTF6-GFP/tt19 that could accumulate anthocyanin and rescue its phenotype,suggesting that MdGSTF6 was an anthocyanin transporter.The silencing of MdGSTF6 affected anthocyanin accumulation in apple fruit.Moreover,the knockdown of MdGSTF6 by RNA interference in cultured‘Gala’seedlings inhibited anthocyanin accumulation.The interaction experiments showed that MdMYB1 could bind directly to the MdGSTF6 promoter to transcriptionally activate its expression.Collectively,our results demonstrate that MdGSTF6 encodes an important GST transporter of anthocyanins in apple fruit and provide evidence for the associated regulatory mechanisms.Therefore,MdMYB1 can not only regulate anthocyanin synthesis,but also control the transport of anthocyanin in apples.This information may be useful for further clarifying the regulation of anthocyanin transport in apple. | Shenghui Jiang Min Chen Naibo He Xiaoliu Chen Nan Wang Qingguo Sun Tianliang Zhang Haifeng Xu Hongcheng Fang Yicheng Wang Zongying Zhang Shujing Wu Xuesen Chen | 2019 | Horticulture Research2019，6，1： | 16 |
| 3 | Genetic Variation of the VP1 Gene of the Virulent Duck Hepatitis A Virus Type 1(DHAV-1) Isolates in Shandong Province of China显示文摘To investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis A virus type 1(DHAV-1) isolates,the virulence,cross neutralization assays and the complete sequence of the virion protein 1(VP1) gene of nine virulent DHAV-1 strains,which were isolated from infected ducklings with clinical symptoms in Shandong province of China in 2007-2008,were tested.The fifth generation duck embryo allantoic liquids of the 9 isolates were tested on 12-day-old duck embryos and on 7-day-old ducklings for the median embryonal lethal doses(ELD 50 s) and the median lethal doses(LD 50 s),respectively.The results showed that the ELD 50 s of embryonic duck eggs of the 9 DHAV-1 isolates were between 1.9 × 10 6 /mL to 1.44 × 10 7 /mL,while the LD 50 s were 2.39 × 10 5 /mL to 6.15 × 10 6 /mL.Cross-neutralization tests revealed that the 9 DHAV-1 isolates were completely neutralized by the standard serum and the hyperimmune sera against the 9 DHAV-1 isolates,respectively.Compared with other virulent,moderate virulent,attenuated vaccine and mild strains,the VP1 genes of the 9 strains shared 89.8%-99.7% similarity at the nucleotide level and 92.4%-99.6% at amino acid level with other DHAV-1 strains.There were three hypervariable regions at the C-terminus(aa 158-160,180-193 and 205-219) and other variable points in VP1 protein,but which didn't cause virulence of DHAV-1 change. | Jiming Gao Junhao Chen Xingkui Si Zhijing Xie Yanli Zhu Xingxiao Zhang Shujing Wang Shijin Jiang | 2012 | Virologica Sinica2012，27，4： | 10 |
| 4 | An approach to quick and easy evaluation of the dam breach flood显示文摘In the context of an impending dam failure, quick evaluation of dam breach flood is necessary. Previous studies provided an approach to calculate the dam breach hydrograph using an Excel spreadsheet based on the improved soil erosion model and numerical algorithm. However, calculation of the breach lateral enlargement requires the modeling of the successive collapse of the breach banks. It is time-consuming with special training, which is difficult to provide during an emergency. This study proposes that the lateral enlargement process can be modeled using a hyperbolic relationship with sufficient accuracy. Consequently, field engineers can perform the dam breach analysis along with the sensitive study for a target case within 1 h in an Excel spreadsheet which is self-tutorial. This paper presents this easy and quick approach based on only fifteen input parameters that can be determined based on the experience. This approach can also be used for the preliminary study when a dam safety planning work is undertaken. | CHEN ZuYu PING ZiYi WANG NaiXin YU Shu CHEN ShuJing | 2019 | Science China(Technological Sciences)2019，62，10： | 9 |
| 5 | Multi-channel micro neural probe fabricated with SOI显示文摘Silicon-on-insulator(SOI) substrate is widely used in micro-electro-mechanical systems(MEMS).With the buried oxide layer of SOI acting as an etching stop,silicon based micro neural probe can be fabri-cated with improved uniformity and manufacturability.A seven-record-site neural probe was formed by inductive-coupled plasma(ICP) dry etching of an SOI substrate.The thickness of the probe is 15 μm.The shaft of the probe has dimensions of 3 mm×100 μm×15 μm with typical area of the record site of 78.5 μm2.The impedance of the record site was measured in-vitro.The typical impedance characteris-tics of the record sites are around 2 MΩ at 1 kHz.The performance of the neural probe in-vivo was tested on anesthetic rat.The recorded neural spike was typically around 140 μV.Spike from individual site could exceed 700 μV.The average signal noise ratio was 7 or more. | PEI WeiHua ZHU Lin WANG ShuJing GUO Kai TANG Jun ZHANG Xu LU Lin GAO ShangKai CHEN HongDa | 2009 | Science China(Technological Sciences)2009，52，5： | 6 |
| 6 | Dual quaternion based virtual hand interaction modeling显示文摘Virtual hands play key roles in three-dimensional interaction in various virtual reality applications.However,their visual realism has seldom been seriously discussed in the community of human computer interaction,not to mention the challenging balance between their visual/motion realism and real-time performance.In this paper,a novel approach for virtual hand interaction modeling based on dual quaternion is proposed.Specifically,dual quaternion blending(DQB) is introduced as pseudo muscular layer of our virtual hand model instead of traditional linear blending(LB) and quaternion blending(QB) due to its advantages in muscular deformation.A framework for virtual hand interaction is proposed which supports both gesture interactions and 3D manipulations.Experimental results show that our proposed virtual hand interaction model is suitable for most virtual reality applications and achieves good visual/motion realism while maintains real-time performance. | CHEN WenFeng ZHU ShuJing WAN HuaGen FENG JieQing | 2013 | Science China(Information Sciences)2013，56，3： | 6 |
| 7 | Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121 in apple显示文摘In plants,the vesicle fusion process plays a vital role in pathogen defence.However,the importance of the vesicle fusion process in apple ring rot has not been studied.Here,we isolated and characterised the apple syntaxin gene MdSYP121.Silencing the MdSYP121 gene in transgenic apple calli increased tolerance to Botryosphaeria dothidea infection;this increased tolerance was correlated with salicylic acid(SA)synthesis-related and signalling-related gene transcription.In contrast,overexpressing MdSYP121 in apple calli resulted in the opposite phenotypes.In addition,the results of RNA sequencing(RNA-Seq)and quantitative real-time PCR(qRT-PCR)assays suggested that MdSYP121 plays an important role in responses to oxidation–reduction reactions.Silencing MdSYP121 in apple calli enhanced the expression levels of reactive oxygen species(ROS)-related genes and the activity of ROS-related enzymes.The enhanced defence response status in MdSYP121-RNAi lines suggests that syntaxins are involved in the defence response to B.dothidea.More importantly,we showed that MdSYP121 forms a soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE)complex with MdSNAP33,and the complex may participate in regulating resistance to B.dothidea.In conclusion,by regulating the interaction of SA pathway and oxidation–reduction process,MdSYP121 can influence the pathogen infection process in apple. | Xiaowen He Yanhong Huo Xiuxia Liu Qianqian Zhou Shouqian Feng Xiang Shen Baohua Li Shujing Wu Xuesen Chen | 2018 | Horticulture Research2018，5，1： | 3 |
| 8 | Implantable CMOS neuro-stimulus chip for visual prosthesis显示文摘A prototype chip with 2×2 pixels for implanting in blind patients affected by outer retinal degeneration is presented in this paper.This visual prosthesis chip imitates the degenerated photoreceptor cells,senses the incident light and stimulates the remaining healthy layers of retina or optic nerve.Each pixel integrates photodiode and stimulus pulse generator,converting the illumination on the eyes into 3-bit resolution bi-phasic current pulses.On-chip charge cancellation modules are used to discharge each electrode site for tissue safety.The prototype chip is designed and fabricated in 0.18-μm N-well CMOS (complementary metal oxide semiconductor) 1P6M Mix-signal process,with a ±2.5 V dual voltage supply.The functionality of the fabricated chip is demonstrated on anesthetized rabbits.Neural responses in visual cortex are successfully evoked by the neuro-stimulus chip through an on-board trigger interface and flexible electrode. | ZHANG Xu PEI WeiHua HUANG BeiJu WANG ShuJing GUAN Ning GUO Kai WANG Yu GUI Qiang CHEN Jin WANG Kai WU HuiJuan LI XiaoXin LI Kai CHEN HongDa | 2011 | Science China(Information Sciences)2011，54，4： | 3 |
| 9 | Protein phosphatase magnesium-dependent 1δ is a novel tumor marker and target in hepatocellular carcinoma显示文摘Hepatocellular 癌(HCC ) 是世界范围的致命的肝恶意。在这研究,我们报导了那个蛋白质磷酸酶镁依赖者(PPM1D ) 1 高度在 HCC 案例(约 59%) 的多数被表示并且显著地把水平与高浆液 -fetoprotein (法新社) 联系了(P = 0.044 ) 。Kaplan- Meier 和考克斯回归数据显示 PPM1D overexpression 是 HCCspecific 全面幸存的一个独立预言者(HR, 2.799;95% CI, 1.346-5.818, P = 0.006 ) 。, Overexpressing PPM1D 支持了房间生存能力和侵略 RNA 调停干扰 PPM1D 击倒有教养的 HCC 房间的禁止的增长,侵略,和移植。另外,由小介入 RNA 的 PPM1D 抑制减少了在 vivo 的 HCC 房间的 tumorigenicity。总的来说,结果建议 PPM1D 为 HCC 是一个潜在的预示的标记和治疗学的目标。 | Zhi Xu Chunxiang Cao Haiyan Xia Shujing Shi Lingzhi Hong Xiaowei Wei Dongying Gu Jianmin Bian Zijun Liu Wenbin Huang Yixin Zhang Song He Nikki Pui-Yue Lee Jinfei Chen | 2016 | Frontiers of Medicine2016，10，1： | 3 |
| 10 | Bevacizumab improves the antitumor efficacy of adoptive cytokine-induced killer cells therapy in non-small cell lung cancer models显示文摘 | Leilei Tao Guichun Huang Shujing Shi Longbang Chen | 2014 | Medical Oncology2014，，1： | 2 |
| 11 | Osteogenesis stimulation by copper-containing 316L stainless steel via activation of akt cell signaling pathway and Runx2 upregulation显示文摘As a metallic orthopedic implant,316L stainless steel(316L SS)is used extensively for its good resistance to corrosion and mechanical properties.However,it takes a long time to achieve osseointegration between 316L SS and adjacent tissues due to its bio-inert characteristic.Hence,the aim is to improve the bio-adaption of 316L SS.A good approach is to add elements to materials to improve their osteogenic capabilities by the appropriate release of ions.Hence copper-containing 316L stainless steel(316L-Cu SS)was investigated in this work,where Cu is an essential trace element that can stimulates osteogenesis.It was found that 316L-Cu SS was bio-safe and did not affect the proliferation of co-cultured osteoblasts in comparison with 316L SS.It increased cell apoptosis on day 1 but inhibited it on day 3,which cooperates with new bone formation processes.Osteoblasts extend themselves more quickly and in a better manner on the surface of 316L-Cu SS,wheneven more pseudopodia are present.Furthermore,the gene expression of alkaline phosphatase,collagen I and runt-related transcription factor 2(Runx2)in osteoblasts cultured with 316L-Cu SS was significantly enhanced.Runx2 protein expression increased,and osteogenesis was stimulated by 316L-Cu SS via an Akt cell signaling pathway.In conclusion,316L-Cu SS stimulates osteogenesis through activation of the Akt cell signaling pathway and the upregulation of Runx2.Thus,316L-Cu SS is a promising material that may be used in surgical implants to stimulate osteogenesis. | Yonghui Yuan Shujing Jin Xun Qi Xudong Chen Wei Zhang Ke Yang Hongshan Zhong | 2019 | Journal of Materials Science & Technology2019，35，11： | 2 |
| 12 | Mapping a mammalian adult adrenal gland hierarchy across species by microwell-seq显示文摘Recently,single-cell RNA-seq technologies have been rapidly updated,leading to a revolution in biology.We previously developed Microwell-seq,a cost-effective and high-throughput single cell RNA sequencing(scRNA-seq)method with a very simple device.Most cDNA libraries are sequenced using an expensive Illumina platform.Here,we present the first report showing combined Microwell-seq and BGI MGISEQ2000,a less expensive sequencing platform,to profile the whole transcriptome of 11,883 individual mouse adult adrenal gland cells and identify 18 transcriptionally distinct clusters.Moreover,we performed a single-cell comparative analysis of human and mouse adult adrenal glands to reveal the conserved genetic networks in these mammalian systems.These results provide new insights into the sophisticated adrenal gland hierarchy and provide a benchmark,low-cost strategy for highthroughput single-cell RNA study. | Shujing Lai Lifeng Ma Weigao E Fang Ye Haide Chen Xiaoping Han Guoji Guo | 2020 | Cell Regeneration2020，9，1： | 2 |
| 13 | Design, Synthesis, Antifungal Activities and SARs of (R)-2-Aryl-4,5-dihydrothiazole-4-carboxylic Acid Derivatives显示文摘基于自然产品 2-aryl-4,5-dihydrothiazole-4-carboxylic 酸的结构,一系列新奇(R)-2-aryl-4,5-dihydrothiazole-4-carboxylic 酸衍生物被设计并且综合。他们的结构被 1 H NMR, 13 C NMR 和 HRMS。复合 9b 的单个水晶结构被 X 光检查衍射分析决定。抗真菌的活动第一次被评估。生物鉴定结果显示展出的大多数混合物节制到好抗真菌的活动。复合 13a 的抗真菌的活动(对 Cercospora arachidicola Hori ) , 13d (对 Alternaria solani ) ,并且 16e (对 Cercospora arachidicola Hori ) 分别地,是 61.9% , 67.3% 和 61.9% 它比商业杀真菌剂 chlorothalonil 和 carbendazim 的那些高。而且,复合 13d 对测试的 7 种真菌展出了优秀抗真菌的活动(66.7% , 77.3% , 63.0% , 87.9% , 70.0% , 70.0% 和 80.0% 在 50 µg ? mL ) 。因此, 13d 能为抗真菌的代理人的发展被用作新铅结构。 | Jingbo Liu Yuxin Li Youwei Chen Xuewen Hua Yingying Wan Wei Wei Haibin Song Shujing Yu Xiao Zhang Zhengming Li | 2015 | Chinese Journal of Chemistry2015，33，11： | 2 |
| 14 | Phase-field simulation of early-stage kinetics evolution ofγ’phase in medium supersaturation Co-Al-W alloy显示文摘The early precipitation ofγ’-Co3(Al,W)phase affects the spatial distribution and kinetic evolution of precipitates for the morphology transmission effect,but the nucleation and concomitant growth are not studied still by aging experiments due to the expeditious precipitation ofγ’phase in Co-Al-W alloy.By using the phase-field simulation with sublattice free energy,the early-stage kinetics evolution ofγ’-Co3(Al,W)phase in a medium supersaturation Co-9 Al-8 W(at.%)alloy aged from 1023 K to 1173 K is investigated.The influences of aging temperature on the evolution of morphology and composition ofγ’phase,and the kinetics of nucleation and growth to coarsening are clarified.It is found that the rates of composition evolution of W inγphase are two or three times that ofγ’phase,and the W compositions inγandγ’phases show a linear relationship with time t–1/3,which means that the coarsening takes place earlier at high temperature.In addition,the equilibrium partitioning ratios indicate Al and W partition into theγ’phase and the ratios decrease with elevated temperature.The compositional variations across theγ/γ’phase interfaces suggest that low aging temperature makes the stoichiometric ratio closer to 3:1.Moreover,the precipitation evolutions from early nucleation to growth and coarsening in Co-Al-W alloy are distinguished,and the rate constants of square and cube of average particles radius increase with temperature.In later growth stage,the relationship of the square of average particles radius and time is obeyed,while the steady-state coarsening stage follows the cube law.The time exponents of particles number density at the coarsening stage are close to–1 of Kuehmann-Voorhees(KV)theory.The study demonstrates that the early-stage evolution ofγ’phase which is undiscovered in the experiment can be captured by the phase-field simulation,and the resultant kinetics laws agree well with the experimental and theoretical results. | Shujing Shi Zhengwei Yan Yongsheng Li Suleman Muhammad Dong Wang Shi Chen Shengshunjin | 2020 | Journal of Materials Science & Technology2020，52，18： | 2 |
| 15 | Development of potent promoters that drive the efficient expression of genes in apple protoplasts显示文摘Protoplast transient expression is a powerful strategy for gene functional characterization,especially in biochemical mechanism studies.We herein developed a highly efficient transient expression system for apple protoplasts.The abilities of the Arabidopsis thaliana and Malus domestica ubiquitin-10(AtUBQ10 and MdUBQ10)promoters to drive the expression of multiple genes were compared with that of the CaMV 35S promoter,and the results revealed that the AtUBQ10 and MdUBQ10 promoters were more efficient in apple protoplasts.With this system,we demonstrated that active AtMKK7ac could activate MAPK6/3/4 signaling cascades,which further regulated MdWRKY33 phosphorylation and stability in apple.Furthermore,the ligand-induced interaction between the immune receptor AtFLS2 and the coreceptor AtBAK1 was reconstituted in apple protoplasts.We also found that the stability of the bacterial effector AvrRpt2 was regulated by feedback involving auxin and the immune regulator RIN4.The system established herein will serve as a useful tool for the molecular and biochemical analyses of apple genes. | Xianpu Wang Lili Xu Xiuxia Liu Li Xin Shujing Wu Xuesen Chen | 2021 | Horticulture Research2021，8，1： | 2 |
| 16 | Pulse vaccination strategy in a delayed SIR epidemic model with vertical transmission显示文摘 | Chen Lansun | 2007 | Discrete and Continuous Dynamical Systems Series B2007，7，： | 1 |
| 17 | Intracoronary Administration of Cardiac Progenitor Cells Alleviates Left Ventricular Dysfunction in Rats With a 30-Day-Old Infarction显示文摘 | Xian-Liang Tang Gregg Rokosh Santosh K. Sanganalmath Fangping Yuan Hiroshi Sato Jianyao Mu Shujing Dai Chengxin Li Ning Chen Yong Peng Buddhadeb Dawn Greg Hunt Annarosa Leri Jan Kajstura Sumit Tiwari Gregg Shirk Piero Anversa Roberto Bolli | 2010 | Circulation2010，，2： | 1 |
| 18 | Solution-processable precursor route for fabricating ultrathin silica film for high performance and low voltage organic transistors显示文摘Silica is one of the most commonly used materials for dielectric layer in organic thin-film transistors due to its excellent stability, excellent electrical properties, mature preparation process, and good compatibility with organic semiconductors. However, most of conventional preparation methods for silica film are generally performed at high temperature and/or high vacuum. In this paper, we introduce a simple solution spin-coating method to fabricate silica thin film from precursor route, which possesses a low leakage current, high capacitance, and low surface roughness. The silica thin film can be produced in the condition of low temperature and atmospheric environment. To meet various demands, the thickness of film can be adjusted by means of preparation conditions such as the speed of spin-coating and the concentration of solution. The p-type and n-type organic field effect transistors fabricated by using this film as gate electrodes exhibit excellent electrical performance including low voltage and high performance. This method shows great potential for industrialization owing to its characteristic of low consumption and energy saving, time-saving and easy to operate. | Shujing Guo Zhongwu Wang Zeyang Xu Shuguang Wang Kunjie Wu Shufeng Chen Zongbo Zhang Caihong Xu Wenfeng Qiu Liqiang Li | 2017 | Chinese Chemical Letters2017，28，11： | 1 |
| 19 | Pulse vaccination of an SEIR epidemic model with time delay 显示文摘 | Gao Shujing Chen Lansun Teng Zhidong | 2008 | Nonlinear Analysis: Real World Applications2008，9，2： | 1 |
| 20 | Achieving laser ignition using zero index metamaterials显示文摘 | Zhai Tianrui Shi Jinwei Chen Shujing | 2011 | Opt Lett2011，36，14： | 1 |
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