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| 1 | A chromosome-scale genome assembly of Artemisia argyi reveals unbiased subgenome evolution and key contributions of gene duplication to volatile terpenoid diversity显示文摘Artemisia argyi Le´vl.et Vant.,a perennial Artemisia herb with an intense fragrance,is widely used in traditional medicine in China and many other Asian countries.Here,we present a chromosome-scale genome assembly of A.argyi comprising 3.89 Gb assembled into 17 pseudochromosomes.Phylogenetic and comparative genomic analyses revealed that A.argyi underwent a recent lineage-specificwhole-genomeduplication(WGD)event after divergence fromArtemisia annua,resulting in two subgenomes.Wedeciphered the diploid ancestral genome of A.argyi,and unbiased subgenome evolution was observed.The recent WGD led to a large number of duplicated genes in the A.argyi genome.Expansion of the terpene synthase(TPS)gene family through various types of gene duplication may have greatly contributed to the diversity of volatile terpenoids in A.argyi.In particular,we identified a typical germacrene D synthase gene cluster within the expanded TPS gene family.The entire biosynthetic pathways of germacrenes,(+)-borneol,and(+)-camphor were elucidated in A.argyi.In addition,partial deletion of the amorpha-4,11-diene synthase(ADS)gene and loss of function of ADS homologs may have resulted in the lack of artemisinin production in A.argyi.Our study provides newinsights into the genome evolution of Artemisia and lays a foundation for further improvement of the quality of this important medicinal plant. | Hongyu Chen Miaoxian Guo Shuting Dong Xinling Wu Guobin Zhang Liu He Yuannian Jiao Shilin Chen Li Li Hongmei Luo | 2023 | Plant Communications2023，4，3： | 2 |
| 2 | Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunity显示文摘Due to the insufficient long-term protection and significant efficacy reduction to new variants of current COVID-19 vaccines,the epidemic prevention and control are still challenging.Here,we employ a capsid and antigen structure engineering(CASE)strategy to manufacture an adenoassociated viral serotype 6-based vaccine(S663V-RBD),which expresses trimeric receptor binding domain(RBD)of spike protein fused with a biological adjuvant RS09.Impressively,the engineered S663V-RBD could rapidly induce a satisfactory RBD-specific IgG titer within 2 weeks and maintain the titer for more than 4 months.Compared to the licensed BBIBP-CorV(Sinopharm,China),a single-dose S663V-RBD induced more endurable and robust immune responses in mice and elicited superior neutralizing antibodies against three typical SARS-CoV-2 pseudoviruses including wild type,C.37(Lambda)and B.1.617.2(Delta).More interestingly,the intramuscular injection of S663V-RBD could overcome pre-existing immunity against the capsid.Given its effectiveness,the CASE-based S663VRBD may provide a new solution for the current and next pandemic. | Fuhua Wu Shuang Luo Yongshun Zhang Yangsen Ou Hairui Wang Zhaofei Guo Chunting He Shuting Bai Penghui He Min Jiang Xiaoyan Chen Guangsheng Du Xun Sun | 2023 | Acta Pharmaceutica Sinica B2023，13，5： | 2 |
| 3 | Size effect of Pt nanoparticles in acid-assisted soot oxidation in the presence of NO显示文摘Pt/Al2O3 catalysts with mean Pt particle size ranged from 2.7 to 7.1 nm were synthesized by chemical reduction method,and the sulfated counterparts were prepared by impregnation of sulfuric acid.The turnover frequency of platinum for soot oxidation under loose contact conditions in a feed flow containing NO and O2 are positively correlated with the size of platinum.The sulfated Pt/Al2O3 exhibits higher catalytic activity for soot oxidation in the presence of NO despite their reduced ability for NO2 production.Such a contradiction is more significant for those catalysts with smaller platinum particles.Herein,the catalysts were characterized by X-ray diffraction(XRD),Brunauer-Emmett-Teller(BET),transmission electron microscopy(TEM),inductive coupled plasma(ICP)emission spectrometry,CO chemisorption,thermogravimetric analysis(TGA),NH3 temperature-programmed desorption(NH 3-TPD),NO temperature-programmed oxidation(TPO)and NO x temperatureprogrammed desorption(TPD).Possible effect of Pt particle size for the catalytic oxidation of soot in the presence of NO was presented based primarily on the promoted NO2 transfer efficiency onto the soot pushed by the acidic catalysts. | Shuting Luo Xiaodong Wu Baofang Jin Shuang Liu Rui Ran Zhichun Si Duan Weng | 2020 | Journal of Environmental Sciences2020，32，8： | 1 |
| 4 | Cause–Effect Relationship between Meso-γ-Scale Rotation and Extreme Short-Term Precipitation:Observational Analyses at Minute and Sub-Kilometer Scales显示文摘The cause–effect relationship between meso-γ-scale rotation and extreme short-term precipitation events remains elusive in mesoscale meteorological research.We aimed to elucidate this relationship by analyzing a rainstorm over the Pearl River Delta during the nocturnal hours of 15 May 2017 based on 6-min radar observations and 1-min rain gauge data.This rainstorm had a maximum hourly rainfall of 100.1 mm,with 26 stations recording hourly rainfall>60 mm h^(−1) in 5 h.Extreme heavy precipitation was produced in association with a convergence zone along the southern side of a synoptic low-level shear line,where southwesterly warm,humid airflows with precipitable water of>60 mm,little convection inhibition(<10 J kg^(−1)),and a low lifting condensation level(about 300 m)dominated.A meso-γ-scale vortex was quantitatively identified during the hour with the largest number of gauges observing extreme hourly rainfall.The vortex had a mean diameter of 6.1 km and a peak intensity of 3.1×10^(−3) s^(−1) during its lifetime of 54 min.The vortex initialized and remained inside the region of extreme rain rates(radar-retrieved rain rates>100 mm h^(−1)),reached its peak intensity after the peak of the collocated 6-min rainfall accumulation,and then weakened rapidly after the extreme rainfall region moved away.The radar-retrieved liquid water path was about five to seven times the ice water path and the specific differential phase(Kdp)below 0°C increased sharply downward during the lifetime of the vortex,suggesting the presence of active warm rain microphysical processes.These results indicate that the release of the latent heat of condensation induced by extreme rainfall could have contributed to the formation of the vortex in an environment with a weak 0–1-km vertical wind shear(about 4–5 m s^(−1))through enhanced lowlevel convergence,although the strengthening of low-level updrafts by rotational dynamic effects and short-term rainfall cannot be ruled out. | Qiuyang ZHANG Yali LUO Ying TANG Xin XU Shuting YU Chong WU | 2022 | Journal of Meteorological Research2022，36，4： | 1 |
| 5 | DOPA-IGF-1 Coated HA/PLGA Microspheres Promoting Proliferation and Osteoclastic Differentiation of Rabbit Bone Mesenchymal Stem Cells显示文摘To explore the ability of dihydroxyphenylalanine-insulin-like growth factor-1 (DOPA-IGF-1) coated hydroxyapatite/poly(lactic-co-glycolic acid)(HA/PLGA) microspheres to promote the proliferation and osteoclastic differentiation of rabbit bone mesenchymal stem cells(rBMSCs), HA/PLGA microspheres with different HA content (10%, 30%, 50%, mass fraction) were prepared by electrospinning method and HA/PLGA microspheres with 50% HA were coated with IGF-1 and DOPA-IGF-1, respectively. They were co-cultured with rBMSCs, respctively. Cell counting kit-8(CCK-8) detection, confocal laser scanning microscopy(CLSM), alkaline phosphatase(ALP) detection and osteogenesis related genes COL IA1, Runx2 and bone morphogenetic protein-2(BMP-2) detection were conducted to detect the proliferation activity, cell morphology, differentiation ability and the expression level of osteogenesis-related genes of cells cultured on all microspheres groups. The results showed that rBMSCs proliferation increased in an HA content dependent manner, and cells proliferated more in the IGF-1 coated and DOPA-IGF-1 coated groups, in particular in DOPA-IGF-1 coated group, and the differences were more remarkable over time (P<0.05). HA/PLGA microspheres promoted the proliferation and osteogenic differentiation of rBMSCs, and DOPA-IGF-1 coating enhanced the proliferation and osteogenic differentiation of rBMSCs. | LIU Guomin LI Yalong YANG Shuting ZHAO Yian LU Tiancheng JIA Wenyuan JI Xuan LUO Yungang | 2019 | Chemical Research in Chinese Universities2019，35，3： | 1 |
| 6 | Implicit Heaviside filter with high continuity based on suitably graded THB splines显示文摘The variable density topology optimization(TO)method has been applied to various engineering fields because it can effectively and efficiently generate the conceptual design for engineering structures.However,it suffers from the problem of low continuity resulting from the discreteness of both design variables and explicit Heaviside filter.In this paper,an implicit Heaviside filter with high continuity is introduced to generate black and white designs for TO where the design space is parameterized by suitably graded truncated hierarchical B-splines(THB).In this approach,the fixed analysis mesh of isogeometric analysis is decoupled from the design mesh,whose adaptivity is implemented by truncated hierarchical B-spline subjected to an admissible requirement.Through the intrinsic local support and high continuity of THB basis,an implicit adaptively adjusted Heaviside filter is obtained to remove the checkboard patterns and generate black and white designs.Threefold advantages are attained in the proposed filter:a)The connection between analysis mesh and adaptive design mesh is easily established compared with the traditional adaptive TO method using nodal density;b)the efficiency in updating design variables is remarkably improved than the traditional implicit sensitivity filter based on B-splines under successive global refinement;and c)the generated black and white designs are preliminarily compatible with current commercial computer aided design system.Several numerical examples are used to verify the effectiveness of the proposed implicit Heaviside filter in compliance and compliant mechanism as well as heat conduction TO problems. | Aodi YANG Xianda XIE Nianmeng LUO Jie ZHANG Ning JIANG Shuting WANG | 2022 | Frontiers of Mechanical Engineering2022，17，1： | 0 |
| 7 | Transcriptomic and genetic approaches reveal that low-light-induced disease susceptibility is related to cellular oxidative stress in tomato显示文摘The impact of low light intensities on plant disease outbreaks represents a major challenge for global crop security,as it frequently results in significant yield losses.However,the underlying mechanisms of the effect of low light on plant defense are still poorly understood.Here,using an RNA-seq approach,we found that the susceptibility of tomato to Pseudomonas syringae pv.tomato DC3000(Pst DC3000)under low light was associated with the oxidation-reduction process.Low light conditions exacerbated Pst DC3000-induced reactive oxygen species(ROS)accumulation and protein oxidation.Analysis of gene expression and enzyme activity of ascorbate peroxidase 2(APX2)and other antioxidant enzymes revealed that these defense responses were significantly induced by Pst DC3000 inoculation under normal light,whereas these genes and their associated enzyme activities were not responsive to pathogen inocula-tion under low light.Additionally,the reduced ascorbate to dehydroascorbate(AsA/DHA)ratio was lower under low light compared with normal light conditions upon Pst DC3000 inoculation.Furthermore,the apx2 mutants generated by a CRISPR-Cas9 gene-editing approach were more susceptible to Pst DC3000 under low light conditions.Notably,this increased susceptibility could be significantly reduced by exogenous AsA treatment.Collectively,our findings suggest that low-light-induced disease susceptibility is associated with increased cellular oxidative stress in tomato plants.This study sheds light on the intricate relationship between light conditions,oxidative stress,and plant defense responses,and may pave the way for improved crop protection strategies in low light environments. | Qian Luo Jiao Wang Ping Wang Xiao Liang Jianxin Li Changqi Wu Hanmo Fang Shuting Ding Shujun Shao Kai Shi | 2023 | Horticulture Research2023，10，10： | 0 |
| 8 | Perilla frutescens:A traditional medicine and food homologous plant显示文摘Perilla frutescens,an annual herb of the Labiatae family,has been cultivated in China for more than 2000 years.P.frutescens is the one of the first medicinal and edible plant published by the Ministry of Health.Its leaves,stems and seeds can be used as medicine and edible food.Because of the abundant nutrients and bioactive components in this plant,P.frutescens has been studied extensively in medicine,food,health care and chemical fields with great prospects for development.This paper reviews the cultivation history,chemical compositions and pharmacological activities of P.frutescens,which provides a reference for the development and utilization of P.frutescens resources. | Xinling Wu Shuting Dong Hongyu Chen Miaoxian Guo Zhiying Sun Hongmei Luo | 2023 | Chinese Herbal Medicines2023，15，3： | 0 |
| 9 | Identification of Monocytes Associated with Severe COVID-19 in the PBMCs of Severely Infected Patients Through Single-Cell Transcriptome Sequencing显示文摘Understanding the immunological characteristics of monocytes-including the characteristics associated with fibrosis-in severe coronavirus disease 2019(COVID-19)is crucial for understanding the pathogenic mechanism of the disease and preventing disease severity.In this study,we performed single-cell transcriptomic sequencing of peripheral blood samples collected from six healthy controls and 14 COVID-19samples including severe,moderate,and convalescent samples from three severely/critically ill and four moderately ill patients.We found that the monocytes were strongly remodeled in the severely/critically ill patients with COVID-19,with an increased proportion of monocytes and seriously reduced diversity.In addition,we discovered two novel severe-disease-specific monocyte subsets:Mono 0 and Mono 5.These subsets expressed amphiregulin(AREG),epiregulin(EREG),and cytokine interleukin-18(IL-18)gene,exhibited an enriched erythroblastic leukemia viral oncogene homolog(ErbB)signaling pathway,and appeared to exhibit pro-fibrogenic and pro-inflammation characteristics.We also found metabolic changes in Mono 0 and Mono 5,including increased glycolysis/gluconeogenesis and an increased hypoxia inducible factor-1(HIF-1)signaling pathway.Notably,one pre-severe sample displayed a monocyte atlas similar to that of the severe/critical samples.In conclusion,our study discovered two novel severedisease-specific monocyte subsets as potential predictors and therapeutic targets for severe COVID-19.Overall,this study provides potential predictors for severe disease and therapeutic targets for COVID-19 and thus provides a resource for further studies on COVID-19. | Yan Zhang Shuting Wang He Xia Jing Guo Kangxin He Chenjie Huang Rui Luo Yanfei Chen Kaijin Xu Hainv Gao Jifang Sheng Lanjuan Li | 2022 | Engineering2022，，10： | 0 |
| 10 | Association Between rs1344706 of ZNF804A and Schizophrenia: A Meta-analysis显示文摘Schizophrenia is one of the most serious mental diseases found in humans. Previous studies indicated that the single nucleotide polymorphism(SNP) rs1344706 in the gene ZNF804 A encoding zinc finger protein 804 A was associated with schizophrenia in Caucasian population but not in Chinese Han population. However, current results are conflicting in Asian population. In the present study, a meta-analysis was performed to revisit the association between rs1344706 and the risk of schizophrenia in Asian, Caucasian and other populations. Electronic search of Pub Med database identified 25 case–control studies with available genotype frequencies of rs1344706 for the meta-analysis,involving a total of 15,788 cases and 22,654 controls. A pooled odds ratio(OR) with 95% confidence interval(CI) was used to assess the association. The current meta-analysis showed an association between rs1344706 and schizophrenia in Caucasian populations(P = 0.028, OR = 1.138, 95% CI:1.014–1.278; P = 0.004 for heterogeneity) and Asian populations(P = 0.008, OR = 1.092, 95%CI: 1.023–1.165; P = 0.001 for heterogeneity), but not in other populations(P = 0.286,OR = 1.209, 95% CI: 0.853–1.714, P = 0.120 for heterogeneity). Egger's test(P > 0.05) and Begg's test(P > 0.05) are both suggestive of the lack of publication bias for the included studies. Thus, the absence of association in other populations suggests a genetic heterogeneity in the susceptibility of schizophrenia and demonstrates the difficulties in replicating genome-wide association study findings regarding schizophrenia across different ethnic populations. To validate the association between rs1344706 and schizophrenia, further studies with larger participant populations worldwide are needed. | Meiyan Zhu Tongyang Liu Jihong Zhang Shuting Jia Wenru Tang Ying Luo | 2014 | Genomics, Proteomics & Bioinformatics2014，12，6： | 0 |
| 11 | Massively efficient filter for topology optimization based on the splitting of tensor product structure显示文摘In this work,we put forward a massively efficient filter for topology optimization(TO)utilizing the splitting of tensor product structure.With the aid of splitting technique,the traditional weight matrices of B-splines and non-uniform rational B-spline implicit filters are decomposed equivalently into two or three submatrices,by which the sensitivity analysis is reformulated for the nodal design variables without altering the optimization process.Afterwards,an explicit sensitivity filter,which is decomposed by the splitting pipeline as that applied to implicit filter,is established in terms of the tensor product of the axial distances between adjacent element centroids,and the corresponding sensitivity analysis is derived for elemental design variables.According to the numerical results,the average updating time for the design variables is accelerated by two-order-of-magnitude for the decomposed filter compared with the traditional filter.In addition,the memory burden and computing time of the weight matrix are decreased by six-and three-order-of-magnitude for the decomposed filter.Therefore,the proposed filter is massively improved by the splitting of tensor product structure and delivers a much more efficient way of solving TO problems in the frameworks of isogeometric analysis and finite element analysis. | Aodi YANG Shuting WANG Nianmeng LUO Tifan XIONG Xianda XIE | 2022 | Frontiers of Mechanical Engineering2022，17，4： | 0 |
| 12 | Strategies on biosynthesis and production of bioactive compounds in medicinal plants显示文摘Medicinal plants are a valuable source of essential medicines and herbal products for healthcare and disease therapy.Compared with chemical synthesis and extraction,the biosynthesis of natural products is a very promising alternative for the successful conservation of medicinal plants,and its rapid development will greatly facilitate the conservation and sustainable utilization of medicinal plants.Here,we summarize the advances in strategies and methods concerning the biosynthesis and production of natural products of medicinal plants.The strategies and methods mainly include genetic engineering,plant cell culture engineering,metabolic engineering,and synthetic biology based on multiple“OMICS”technologies,with paradigms for the biosynthesis of terpenoids and alkaloids.We also highlight the biosynthetic approaches and discuss progress in the production of some valuable natural products,exemplifying compounds such as vindoline(alkaloid),artemisinin and paclitaxel(terpenoids),to illustrate the power of biotechnology in medicinal plants. | Miaoxian Guo Haizhou Lv Hongyu Chen Shuting Dong Jianhong Zhang Wanjing Liu Liu He Yimian Ma Hua Yu Shilin Chen Hongmei Luo | 2024 | Chinese Herbal Medicines2024，16，1： | 0 |
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