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| 1 | Inhibition of hepatitis C virus replication by single-stranded RNA structural mimics显示文摘AIM: To examine the effect of hepatitis C virus (HCV) structural mimics of regulatory regions of the genome on HCV replication.METHODS: HCV RNA structural mimics were constructed and tested in a HCV genotype 1b aBB7 replicon,and a Japanese fulminant hepatitis-1 (JFH-1) HCV genotype 2a infection model.All sequences were computer-predicted to adopt stem-loop structures identical to the corresponding elements in full-length viral RNA.Huh7.5 cells bearing the BB7 replicon or infected with JFH-1 virus were transfected with expression vectors generating HCV mimics and controls.Cellular HCV RNA and protein levels were quantified by real-time polymerase chain reaction and Western blotting,respectively.To evaluate possible antisense effects,complementary RNAs spanning a mimic were prepared.RESULTS: In the BB7 genotype 1b replicon system,mimics of the polymerase (NS-5B),X and BA regions inhibited replication by more than 90%,50%,and 60%,respectively.In the JFH-1 genotype 2 infection system,mimics that were only 74% and 46% identical in sequence relative to the corresponding region in JFH-1 inhibited HCV replication by 91.5% and 91.2%,respectively,as effectively as a mimic with complete identity to HCV genotype 2a.The inhibitory effects were confirmed by NS3 protein levels.Antisense RNA molecules spanning the 74% identical mimic had no significant effects.CONCLUSION: HCV RNA structural mimics can inhibit HCV RNA replication in replicon and infectious HCV systems and do so independent of close sequence identity with the target. | Robert Smolic Martina Smolic John H Andorfer Catherine H Wu Robert M Smith George Y Wu | 2010 | World Journal of Gastroenterology2010，16，17： | 2 |
| 2 | Automatic generation of redundant models for permutation constraint satisfaction problems显示文摘 | Law Y C Lee J H M Smith B M | 2007 | Constraints2007，12，4： | 1 |
| 3 | Vanadium alloys-overview and recent results 显示文摘 | Muroga T Nagasaka T Abe K Chemov V M Matsui H Smith D L Xu Z Y Zinkle S J | 2002 | Journal of Nuclear Materials2002，，307311： | 1 |
| 4 | Quality of minimally processed apple slices from selected cultivars 显示文摘 | Kim D M Smith N L Lee C Y | 1993 | Journal of Food Science1993，58，5： | 1 |
| 5 | Bounds testing approaches to the analysis of level relationships 显示文摘 | PESARAN M H SHIN Y SMITH R J | 2001 | Journal of Applied Economet- rics2001，，16： | 1 |
| 6 | Primary porcine brain microvas- cular endothelial cells:biochemical and functional characterisation as a model for drug transport and targeting显示文摘 | Smith M Omidi Y Gumbleton M | 2007 | J Drug Target2007，15，4： | 1 |
| 7 | Ultrasonic velocities of the dilatational and shear waves in frozen soils显示文摘 | NAKANO Y MARTIN A J SMITH M | 1972 | W~/ter Resources Research1972，8，4： | 1 |
| 8 | A randomized controlledtrial comparing intravenous ferric carboxymaltose with oral iron fortreatment of iron deficiency anaemia of non-dialysis-dependent chronickidney disease patients 显示文摘 | Qunibi W Y Martinez C Smith M | 2011 | Nephrol Dial Transplant2011，26，5： | 1 |
| 9 | Transformation of bahiagrass(Paspalum notatum Flugge)显示文摘 | SMITH R L GRANDO M F LI Y Y | 2002 | Plant Cell Rep2002，20，： | 1 |
| 10 | Static and periodic infinite solid elements 显示文摘 | Chow Y K Smith I M | 1981 | International Journal for Numerical Methods in Engineering1981，17，4： | 1 |
| 11 | The Mycobacterium tuberculosis protein serine/threonine kinase PknG is linked to cellular glutamate/glutamine levels and is important for growth in vivo显示文摘 | Cowley S Ko M Pick N Chow R Downing KJ Gordhan BG Betts JC Mizrahi V Smith DA Stokes RW Av-Gay Y | 2004 | Mol Microbiol2004，52，6： | 1 |
| 12 | Ides: an internet distanceestimation service for large networks显示文摘 | MAO Y SAUL L K SMITH J M | 2006 | IEEE Journal onSelected Areas in Communications2006，24，12： | 1 |
| 13 | Continuous cell culture and product recovery from wild Vaccinium pahalae 显示文摘 | Smith M A Madhavi D L Fang Y | 1997 | J Plant Physiol1997，150，： | 1 |
| 14 | Novel M(II)-Hg(II) coordination polymers generated from metal-containing building blocks M (2-pyrazineearboxyliate)2 '(H2O)2 (M=Cu,Ni,Co)and HgCl2显示文摘 | Dong Y B Smith M D zur Loye H C | 2000 | Solid State Sci2000，，2： | 1 |
| 15 | Zotarolimus, a novel sirolimus analogue with potent anti-proliferative activity on coronary smooth muscle cells and reduced potential for systemic immunosuppression显示文摘 | Chen Y W Smith M L Sheets M | 2007 | J Cardiovasc Pharmacol2007，49，： | 1 |
| 16 | A comparison of the shock index and conventional vital signs to identify acute, critical illness in the emergency department显示文摘 | RADY M Y SMITH LINE H A BLAKE H | 1994 | Am Emerg Med1994，24，： | 1 |
| 17 | Thereactivity pat- terns of low-coordinate iron-hydride complexes 显示文摘 | Yu Y Sadique A R Smith J M | 2008 | J Am Chern Soc2008，130，： | 1 |
| 18 | Accurate,robust,and automated longitudinal and cross-sectional brain change analysis显示文摘 | Smith S Zhang Y Jenkinson M | 2002 | NeuroImage2002，17，1： | 1 |
| 19 | High incidence of positive peritoneal cytology in low - risk endometrial cancer treated by lapareseopically assisted vaginal hysterectomy显示文摘 | Sonoda Y Zerbe M Smith A | 2001 | Gynecol Oncol2001，80，3： | 1 |
| 20 | Dissipation of sulfamethoxazole and trimethoprim antibiotics from manure显示文摘 | Wu Y Williams M Smith L | 2012 | J Environ Sci Health B2012，47，4： | 1 |
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